27b13ee02c2814b1bb933275f63cd406 pmc_34204120.pdf fb7e1a62c503889624740d0adce5bd72ad8cd937 pmc_34204120.pdf b0da513de52e784f4225b654cbaf687aeec2f9a6dc9ddd53581ff2866af8f9e9 pmc_34204120.pdf Title: Fujifilm SILVAMP TB-LAM for the Diagnosis of Tuberculosis in Nigerian Adults Subject: There is a need for diagnostics for tuberculosis (TB) that are easy to use, able to screen non-sputum samples, and able to provide rapid results for the management of both immunocompromised and immunocompetent individuals. The Fujifilm SILVAMP TB LAM (FujiLAM) assay, a new non-sputum based point of need test for the diagnosis of TB, could potentially address most of these needs. We evaluated the performance of FujiLAM in HIV positive and HIV negative patients with presumptive TB attending three district hospitals in Nigeria. Consecutive patients were asked to provide urine samples on the spot, which were tested with FujiLAM. The results were compared against a positive culture and/or Xpert MTB/RIF as the reference standard. Forty-five patients had bacteriologically confirmed TB, and 159 had negative culture and Xpert MTB/RIF (no TB). The FujiLAM test was positive in 23 (sensitivity 65.7%, 95% CI = 48–80) HIV negative and seven (70%, 95% CI = 35–92) HIV positive patients with bacteriological confirmation of TB. FujiLAM was negative in 97 (specificity 99.0%, 95% CI = 94–100) HIV negative and 56 (93.3%, 95% CI = 83–98) HIV positive patients without TB. The FujiLAM test has good diagnostic accuracy for considering its application in both HIV positive and HIV negative patients with TB. Keywords: tuberculosis; diagnosis; lipoarabinomannan; LAM; HIV; urine; point-of-care Author: Patricia Comella-del-Barrio, John S. Bimba, Ramota Adelakun, Konstantina Kontogianni, Bárbara Molina-Moya, Okoedoh Osazuwa, Jacob Creswell, Luis E. Cuevas and José Domínguez Creator: LaTeX with hyperref Producer: pdfTeX-1.40.21 CreationDate: Tue Jun 8 03:11:13 2021 CEST ModDate: Tue Jun 8 03:17:42 2021 CEST Tagged: no UserProperties: no Suspects: no Form: none JavaScript: no Pages: 9 Encrypted: no Page size: 595.276 x 841.89 pts (A4) Page rot: 0 File size: 385982 bytes Optimized: no PDF version: 1.7 name type encoding emb sub uni object ID ------------------------------------ ----------------- ---------------- --- --- --- --------- DDMOHK+VnURWPalladioL Type 1 Custom yes yes no 10 0 UAWVTV+URWPalladioL-Ital Type 1 Custom yes yes no 15 0 KVUFOL+URWPalladioL-Bold Type 1 Custom yes yes no 20 0 LKIJPP+URWPalladioL-Roma Type 1 Custom yes yes no 24 0 IOJCNK+VnURWPalladioL-Bold Type 1 Custom yes yes no 28 0 XPUSBJ+TimesNewRomanPS-BoldItalicMT Type 1C WinAnsi yes yes no 36 0 BSDWLC+TimesNewRomanPSMT Type 1C WinAnsi yes yes no 39 0 MCBCCI+SourceSansRoman-Regular Type 1C Custom yes yes no 61 0 MCBCCM+SourceSansRoman.613wght Type 1C Custom yes yes no 65 0 MCBCCO+SourceSansRoman-Bold Type 1C Custom yes yes no 69 0 QLBIEB+URWPalladioL-BoldItal Type 1 Custom yes yes no 91 0 Jhove (Rel. 1.22.1, 2019-04-17) Date: 2021-09-11 03:06:04 CEST RepresentationInformation: pmc_34204120.pdf ReportingModule: PDF-hul, Rel. 1.12.1 (2019-04-17) LastModified: 2021-07-05 03:36:40 CEST Size: 385982 Format: PDF Version: 1.7 Status: Well-Formed and valid SignatureMatches: PDF-hul MIMEtype: application/pdf PDFMetadata: Objects: 204 FreeObjects: 1 IncrementalUpdates: 0 DocumentCatalog: PageLayout: SinglePage PageMode: UseNone Outlines: Item: Title: Introduction Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@13c27452 Item: Title: Materials and Methods Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@262b2c86 Item: Title: Results Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@371a67ec Item: Title: Discussion Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@5ed828d Item: Title: References Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@50d0686 Info: Title: Fujifilm SILVAMP TB-LAM for the Diagnosis of Tuberculosis in Nigerian Adults Author: Patricia Comella-del-Barrio, John S. Bimba, Ramota Adelakun, Konstantina Kontogianni, Bárbara Molina-Moya, Okoedoh Osazuwa, Jacob Creswell, Luis E. Cuevas and José Domínguez Subject: There is a need for diagnostics for tuberculosis (TB) that are easy to use, able to screen non-sputum samples, and able to provide rapid results for the management of both immunocompromised and immunocompetent individuals. The Fujifilm SILVAMP TB LAM (FujiLAM) assay, a new non-sputum based point of need test for the diagnosis of TB, could potentially address most of these needs. We evaluated the performance of FujiLAM in HIV positive and HIV negative patients with presumptive TB attending three district hospitals in Nigeria. Consecutive patients were asked to provide urine samples on the spot, which were tested with FujiLAM. The results were compared against a positive culture and/or Xpert MTB/RIF as the reference standard. Forty-five patients had bacteriologically confirmed TB, and 159 had negative culture and Xpert MTB/RIF (no TB). The FujiLAM test was positive in 23 (sensitivity 65.7%, 95% CI = 48 80) HIV negative and seven (70%, 95% CI = 35 92) HIV positive patients with bacteriological confirmation of TB. FujiLAM was negative in 97 (specificity 99.0%, 95% CI = 94 100) HIV negative and 56 (93.3%, 95% CI = 83 98) HIV positive patients without TB. The FujiLAM test has good diagnostic accuracy for considering its application in both HIV positive and HIV negative patients with TB. 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