c6033085a345b504c43ba4d7874e51a0 pmc_34300220.pdf c2c7ae7428dc8b3c47133ba0b290e8d5e8b72480 pmc_34300220.pdf 3bad6153c9d691ec5b75656b161a8c2b3a54c5f021a9a36271b0200d503d895e pmc_34300220.pdf Title: Growth Hormone (GH) Treatment Decreases Plasma Kisspeptin Levels in GH-Deficient Adults with Prader–Willi Syndrome Subject: Obesity and growth hormone (GH)-deficiency are consistent features of Prader–Willi syndrome (PWS). Centrally, kisspeptin is involved in regulating reproductive function and can stimulate hypothalamic hormones such as GH. Peripherally, kisspeptin signaling influences energy and metabolic status. We evaluated the effect of 12-month GH treatment on plasma kisspeptin levels in 27 GH-deficient adult PWS patients and analyzed its relationship with metabolic and anthropometric changes. Twenty-seven matched obese subjects and 22 healthy subjects were also studied. Before treatment, plasma kisspeptin concentrations in PWS and obese subjects were similar (140.20 (23.5–156.8) pg/mL vs. 141.96 (113.9–165.6) pg/mL, respectively, p = 0.979)) and higher (p = 0.019) than in healthy subjects (124.58 (107.3–139.0) pg/mL); plasma leptin concentrations were similar in PWS and obese subjects (48.15 (28.80–67.10) ng/mL vs. 33.10 (20.50–67.30) ng/mL, respectively, p = 0.152) and higher (p < 0.001) than in healthy subjects (14.80 (11.37–67.30) ng/mL). After GH therapy, lean body mass increased 2.1% (p = 0.03), total fat mass decreased 1.6% (p = 0.005), and plasma kisspeptin decreased to levels observed in normal-weight subjects (125.1(106.2–153.4) pg/mL, p = 0.027). BMI and leptin levels remained unchanged. In conclusion, 12-month GH therapy improved body composition and decreased plasma kisspeptin in GH deficient adults with PWS. All data are expressed in median (interquartile range). Keywords: Prader–Willi Syndrome; kisspeptin; leptin; growth hormone deficiency Author: Olga Giménez-Palop, Laia Casamitjana, Raquel Corripio, Susanna Esteba-Castillo, Rocío Pareja, Néstor Albiñana, Mercedes Rigla and Assumpta Caixàs Creator: LaTeX with hyperref Producer: pdfTeX-1.40.21 CreationDate: Tue Jul 13 09:54:24 2021 CEST ModDate: Tue Jul 13 10:00:29 2021 CEST Custom Metadata: no Metadata Stream: no Tagged: no UserProperties: no Suspects: no Form: none JavaScript: no Pages: 11 Encrypted: no Page size: 595.276 x 841.89 pts (A4) Page rot: 0 File size: 862079 bytes Optimized: no PDF version: 1.7 name type encoding emb sub uni object ID ------------------------------------ ----------------- ---------------- --- --- --- --------- FJLSMC+VnURWPalladioL Type 1 Custom yes yes no 10 0 ONOYFO+URWPalladioL-Ital Type 1 Custom yes yes no 15 0 SDMGQX+URWPalladioL-Bold Type 1 Custom yes yes no 20 0 YNGUSP+URWPalladioL-Roma Type 1 Custom yes yes no 24 0 GGHKEL+VnURWPalladioL-Bold Type 1 Custom yes yes no 28 0 XPUSBJ+TimesNewRomanPS-BoldItalicMT Type 1C WinAnsi yes yes no 36 0 BSDWLC+TimesNewRomanPSMT Type 1C WinAnsi yes yes no 39 0 MCBCCI+SourceSansRoman-Regular Type 1C Custom yes yes no 61 0 MCBCCM+SourceSansRoman.613wght Type 1C Custom yes yes no 65 0 MCBCCO+SourceSansRoman-Bold Type 1C Custom yes yes no 69 0 OHXQCJ+CMSY10 Type 1 Builtin yes yes no 81 0 QLBIEB+URWPalladioL-BoldItal Type 1 Custom yes yes no 89 0 NXQZWG+EURM10 Type 1 Builtin yes yes no 93 0 BCDEEE+PalatinoLinotype-Bold TrueType WinAnsi yes yes no 101 0 BCDFEE+PalatinoLinotype-Roman TrueType WinAnsi yes yes no 105 0 BCDGEE+PalatinoLinotype-Italic TrueType WinAnsi yes yes no 109 0 BCDIEE+PalatinoLinotype-Roman CID TrueType Identity-H yes yes yes 113 0 BCDJEE+PalatinoLinotype-BoldItalic TrueType WinAnsi yes yes no 120 0 BCDKEE+Sylfaen CID TrueType Identity-H yes yes yes 124 0 CEBOHO+PalatinoLinotype,Bold TrueType WinAnsi yes yes no 143 0 CEBOHP+PalatinoLinotype CID TrueType Identity-H yes yes yes 146 0 CEBOIA+PalatinoLinotype TrueType WinAnsi yes yes no 152 0 CEBOIC+PalatinoLinotype,Italic TrueType WinAnsi yes yes no 155 0 CECAMH+Sylfaen CID TrueType Identity-H yes yes yes 158 0 CEBOHO+PalatinoLinotype,Bold TrueType WinAnsi yes yes no 173 0 CEBOIA+PalatinoLinotype TrueType WinAnsi yes yes no 176 0 CEBOIC+PalatinoLinotype,Italic TrueType WinAnsi yes yes no 179 0 CECAKH+PalatinoLinotype,BoldItalic TrueType WinAnsi yes yes no 182 0 Jhove (Rel. 1.28.0, 2023-05-18) Date: 2024-10-02 03:10:12 CEST RepresentationInformation: pmc_34300220.pdf ReportingModule: PDF-hul, Rel. 1.12.4 (2023-03-16) LastModified: 2023-03-31 12:14:35 CEST Size: 862079 Format: PDF Version: 1.7 Status: Well-Formed and valid SignatureMatches: PDF-hul MIMEtype: application/pdf PDFMetadata: Objects: 346 FreeObjects: 1 IncrementalUpdates: 0 DocumentCatalog: PageLayout: SinglePage PageMode: UseNone Outlines: Item: Title: Introduction Destination: section.1 Item: Title: Materials and Methods Destination: section.2 Children: Item: Title: Subjects Destination: subsection.2.1 Item: Title: Methods Destination: subsection.2.2 Item: Title: Statistical Analyses Destination: subsection.2.3 Item: Title: Results Destination: section.3 Children: Item: Title: Baseline Findings Destination: subsection.3.1 Children: Item: Title: Patient Characteristics Destination: subsubsection.3.1.1 Item: Title: Kisspeptin Levels Destination: subsubsection.3.1.2 Item: Title: Leptin Levels Destination: subsubsection.3.1.3 Item: Title: Comparison between Baseline and Post-GH-Treatment Findings in the PWS Group Destination: subsection.3.2 Item: Title: Correlations Destination: section.4 Item: Title: Discussion Destination: section.5 Item: Title: References Destination: section.5 Info: Title: Growth Hormone (GH) Treatment Decreases Plasma Kisspeptin Levels in GH-Deficient Adults with Prader Willi Syndrome Author: Olga Giménez-Palop, Laia Casamitjana, Raquel Corripio, Susanna Esteba-Castillo, Rocío Pareja, Néstor Albiñana, Mercedes Rigla and Assumpta Caixàs Subject: Obesity and growth hormone (GH)-deficiency are consistent features of Prader Willi syndrome (PWS). Centrally, kisspeptin is involved in regulating reproductive function and can stimulate hypothalamic hormones such as GH. Peripherally, kisspeptin signaling influences energy and metabolic status. We evaluated the effect of 12-month GH treatment on plasma kisspeptin levels in 27 GH-deficient adult PWS patients and analyzed its relationship with metabolic and anthropometric changes. Twenty-seven matched obese subjects and 22 healthy subjects were also studied. Before treatment, plasma kisspeptin concentrations in PWS and obese subjects were similar (140.20 (23.5 156.8) pg/mL vs. 141.96 (113.9 165.6) pg/mL, respectively, p = 0.979)) and higher (p = 0.019) than in healthy subjects (124.58 (107.3 139.0) pg/mL); plasma leptin concentrations were similar in PWS and obese subjects (48.15 (28.80 67.10) ng/mL vs. 33.10 (20.50 67.30) ng/mL, respectively, p = 0.152) and higher (p < 0.001) than in healthy subjects (14.80 (11.37 67.30) ng/mL). After GH therapy, lean body mass increased 2.1% (p = 0.03), total fat mass decreased 1.6% (p = 0.005), and plasma kisspeptin decreased to levels observed in normal-weight subjects (125.1(106.2 153.4) pg/mL, p = 0.027). BMI and leptin levels remained unchanged. In conclusion, 12-month GH therapy improved body composition and decreased plasma kisspeptin in GH deficient adults with PWS. All data are expressed in median (interquartile range). 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