633ecc7e7bbf3b73a591157e266bff84 pmc_34358167.pdf 6f92b4a04b1dade4c66b83cee64f7b6d2d4408b2 pmc_34358167.pdf 11386c80ac528bdeb8a351ad3feddf7f932722867e6bac0053d6d49710a95858 pmc_34358167.pdf Title: Immune Responses to Pandemic H1N1 Influenza Virus Infection in Pigs Vaccinated with a Conserved Hemagglutinin HA1 Peptide Adjuvanted with CAF®01 or CDA/GalCerMPEG Subject: This study aimed to evaluate the immune response and protection correlates against influenza virus (IV) infection in pigs vaccinated with the novel NG34 HA1 vaccine candidate adjuvanted with either CAF®01 or CDA/GalCerMPEG (GCM). Two groups of six pigs each were vaccinated intramuscularly twice with either NG34 + CAF®01 or NG34 + CDA/GCM. As controls, groups of animals (n = 6 or 4) either non-vaccinated or vaccinated with human seasonal trivalent influenza vaccine or NG34 + Freund’s adjuvant were included in the study. All animal groups were challenged with the 2009 pandemic (pdm09) strain of H1N1 (total amount of 7 106 TCID50/mL) via intranasal and endotracheal routes 21 days after second vaccination. Reduced consolidated lung lesions were observed both on days three and seven post-challenge in the animals vaccinated with NG34 + CAF®01, whereas higher variability with relatively more severe lesions in pigs of the NG34 + CDA/GCM group on day three post-infection. Among groups, animals vaccinated with NG34 + CDA/GCM showed higher viral loads in the lung at seven days post infection whereas animals from NG34 + CAF®01 completely abolished virus from the lower respiratory tract. Similarly, higher IFN secretion and stronger IgG responses against the NG34 peptide in sera was observed in animals from the NG34 + CAF®01 group as compared to the NG34 + CDA/GCM. NG34-vaccinated pigs with adjuvanted CAF®01 or CDA/GCM combinations resulted in different immune responses as well as outcomes in pathology and viral shedding. Keywords: vaccine; adjuvants; influenza; immunity; pathology Author: Sergi López-Serrano, Lorena Cordoba, Mónica Pérez-Maillo, Patricia Pleguezuelos, Edmond J. Remarque, Thomas Ebensen, Carlos A. Guzmán, Dennis Christensen, Joaquim Segalés and Ayub Darji Creator: LaTeX with hyperref Producer: pdfTeX-1.40.21 CreationDate: Thu Jul 8 08:07:46 2021 CEST ModDate: Thu Jul 8 08:12:53 2021 CEST Tagged: no UserProperties: no Suspects: no Form: none JavaScript: no Pages: 17 Encrypted: no Page size: 595.276 x 841.89 pts (A4) Page rot: 0 File size: 2287596 bytes Optimized: no PDF version: 1.7 name type encoding emb sub uni object ID ------------------------------------ ----------------- ---------------- --- --- --- --------- ZZTQUO+VnURWPalladioL-Bold Type 1 Custom yes yes no 10 0 IHGURL+EURM7 Type 1 Builtin yes yes no 15 0 DAJWUC+VnURWPalladioL Type 1 Custom yes yes no 19 0 KNPFEX+EURM10 Type 1 Builtin yes yes no 23 0 WCLVMK+URWPalladioL-Ital Type 1 Custom yes yes no 27 0 WDGZCD+URWPalladioL-Bold Type 1 Custom yes yes no 32 0 RMFQQF+URWPalladioL-Roma Type 1 Custom yes yes no 36 0 QMDDXF+CMSY10 Type 1 Builtin yes yes no 40 0 CZOINJ+EURB10 Type 1 Builtin yes yes no 44 0 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UseNone Outlines: Item: Title: Introduction Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@13c27452 Item: Title: Materials and Methods Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@262b2c86 Children: Item: Title: Vaccine Antigens and Adjuvants Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@371a67ec Item: Title: Cell Cultures and Virus Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@5ed828d Item: Title: Experimental Design Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@50d0686 Item: Title: Sampling Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@7a3d45bd Item: Title: Clinical Signs and Pathological Assessment Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@1e7c7811 Item: Title: Quantitative PCR RT-qPCR Viral Load Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@77ec78b9 Item: Title: Assessment of IFN Producing Cells Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@1a3869f4 Item: Title: Humoral Immune Response Evaluation Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@a38d7a3 Item: Title: Hemagglutination Inhibition and Neutralization Assays Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@77f99a05 Item: Title: Statistical Analysis Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@63440df3 Item: Title: Results Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@3aeaafa6 Children: Item: Title: Clinical Signs and Pathology Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@76a3e297 Item: Title: RT-qPCR Results Viral Load Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@4d3167f4 Item: Title: Cell Immune Response-ELISPOT Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@ed9d034 Item: Title: Antibody Response Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@6121c9d6 Item: Title: Hemagglutination Inhibition and Neutralizing Antibodies Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@87f383f Item: Title: Discussion Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@4eb7f003 Item: Title: Conclusions Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@eafc191 Item: Title: Patents Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@612fc6eb Item: Title: References Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@1060b431 Info: Title: Immune Responses to Pandemic H1N1 Influenza Virus Infection in Pigs Vaccinated with a Conserved Hemagglutinin HA1 Peptide Adjuvanted with CAF®01 or CDA/GalCerMPEG Author: Sergi López-Serrano, Lorena Cordoba, Mónica Pérez-Maillo, Patricia Pleguezuelos, Edmond J. Remarque, Thomas Ebensen, Carlos A. Guzmán, Dennis Christensen, Joaquim Segalés and Ayub Darji Subject: This study aimed to evaluate the immune response and protection correlates against influenza virus (IV) infection in pigs vaccinated with the novel NG34 HA1 vaccine candidate adjuvanted with either CAF®01 or CDA/GalCerMPEG (GCM). Two groups of six pigs each were vaccinated intramuscularly twice with either NG34 + CAF®01 or NG34 + CDA/GCM. As controls, groups of animals (n = 6 or 4) either non-vaccinated or vaccinated with human seasonal trivalent influenza vaccine or NG34 + Freund’s adjuvant were included in the study. All animal groups were challenged with the 2009 pandemic (pdm09) strain of H1N1 (total amount of 7 106 TCID50/mL) via intranasal and endotracheal routes 21 days after second vaccination. Reduced consolidated lung lesions were observed both on days three and seven post-challenge in the animals vaccinated with NG34 + CAF®01, whereas higher variability with relatively more severe lesions in pigs of the NG34 + CDA/GCM group on day three post-infection. Among groups, animals vaccinated with NG34 + CDA/GCM showed higher viral loads in the lung at seven days post infection whereas animals from NG34 + CAF®01 completely abolished virus from the lower respiratory tract. Similarly, higher IFN secretion and stronger IgG responses against the NG34 peptide in sera was observed in animals from the NG34 + CAF®01 group as compared to the NG34 + CDA/GCM. NG34-vaccinated pigs with adjuvanted CAF®01 or CDA/GCM combinations resulted in different immune responses as well as outcomes in pathology and viral shedding. 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