e0f5a798fb371d5a97d973f561348236 pmc_34359143.pdf 4ebb0abfaf580887b7bd381f123b9a0020ee24ad pmc_34359143.pdf 7de0237c125e7df45211a894c1f3a677f4982803ed008b27ce9c8f2d5764067d pmc_34359143.pdf Title: Evaluation of Plasmatic Procalcitonin in Healthy, and in Systemic Inflammatory Response Syndrome (SIRS) Negative or Positive Colic Horses Subject: Colic horses show systemic inflammatory response syndrome (SIRS) clinical signs. Procalcitonin (PCT) showed increased circulating levels in sick horses. This study compares plasma PCT concentrations in healthy vs. SIRS negative/positive colic horses over time, and evaluates PCT and SIRS score potential correlation, to verify the usefulness of PCT for the evaluation of SIRS severity. Ninety-one horses were included; 43/91 were healthy, on basis of physical examination, blood work and SIRS score (score = 0), while 48/91 were sick colic horses, classified as SIRS-negative (score < 2) and positive (score 2). Moreover, a 0–6 point-scale SIRS score was calculated (assessing mucous membrane color and blood lactate concentration). PCT was evaluated at admission, and at 24, 48, 72 and 96 h, using a commercial kit for equine species. We verified by the ANOVA test PCT differences between healthy vs. colic horses, healthy vs. SIRS-negative or SIRS-positive colic horses, at all sampling times, and the correlation between the SIRS score at admission with the SIRS score. Statistically significant differences were detected between healthy vs. all colic horses and between healthy vs. SIRS-positive or negative horses at all sampling times. No correlation was observed between the SIRS score at admission and PCT values. PCT was statistically higher in colic horses compared to the healthy ones, suggesting a role as a biomarker for colic. Keywords: horse; colic; procalcitonin; systemic inflammatory response syndrome Author: Irene Nocera, Francesca Bonelli, Valentina Vitale, Valentina Meucci, Giuseppe Conte, Eduard Jose-Cunilleras, Luis Alfonso Gracia-Calvo and Micaela Sgorbini Creator: LaTeX with hyperref Producer: pdfTeX-1.40.21 CreationDate: Tue Jul 6 07:42:31 2021 CEST ModDate: Tue Jul 6 07:59:57 2021 CEST Tagged: no UserProperties: no Suspects: no Form: none JavaScript: no Pages: 9 Encrypted: no Page size: 595.276 x 841.89 pts (A4) Page rot: 0 File size: 564880 bytes Optimized: no PDF version: 1.7 name type encoding emb sub uni object ID ------------------------------------ ----------------- ---------------- --- --- --- --------- KRPFHL+URWPalladioL-Ital Type 1 Custom yes yes no 10 0 KLQAFM+URWPalladioL-Bold Type 1 Custom yes yes no 15 0 JABTBZ+URWPalladioL-Roma Type 1 Custom yes yes no 19 0 ZCWVNZ+CMSY10 Type 1 Builtin yes yes no 23 0 SZMRXJ+TimesNewRomanPS-BoldItalicMT Type 1C WinAnsi yes yes no 31 0 MCBCCI+SourceSansRoman-Regular Type 1C Custom yes yes no 53 0 MCBCCM+SourceSansRoman.613wght Type 1C Custom yes yes no 57 0 MCBCCO+SourceSansRoman-Bold Type 1C Custom yes yes no 61 0 OVNEXW+EURM10 Type 1 Builtin yes yes no 76 0 JHYTSG+CMR10 Type 1 Builtin yes yes no 89 0 NGONCI+PalatinoLinotype,Bold TrueType WinAnsi yes yes no 100 0 NGONCJ+PalatinoLinotype CID TrueType Identity-H yes yes yes 103 0 NGONCK+PalatinoLinotype TrueType WinAnsi yes yes no 109 0 NGONDM+PalatinoLinotype,Italic TrueType WinAnsi yes yes no 112 0 Jhove (Rel. 1.22.1, 2019-04-17) Date: 2023-05-27 02:42:16 CEST RepresentationInformation: pmc_34359143.pdf ReportingModule: PDF-hul, Rel. 1.12.1 (2019-04-17) LastModified: 2023-03-31 12:14:45 CEST Size: 564880 Format: PDF Version: 1.7 Status: Well-Formed and valid SignatureMatches: PDF-hul MIMEtype: application/pdf PDFMetadata: Objects: 258 FreeObjects: 1 IncrementalUpdates: 0 DocumentCatalog: PageLayout: SinglePage PageMode: UseNone Outlines: Item: Title: Introduction Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@44c8afef Item: Title: Materials and Methods Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@7b69c6ba Children: Item: Title: Study Population Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@46daef40 Item: Title: PCT Evaluation Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@12f41634 Item: Title: Statistical Analysis Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@13c27452 Item: Title: Results Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@262b2c86 Item: Title: Discussion Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@371a67ec Item: Title: Conclusions Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@5ed828d Item: Title: References Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@50d0686 Info: Title: Evaluation of Plasmatic Procalcitonin in Healthy, and in Systemic Inflammatory Response Syndrome (SIRS) Negative or Positive Colic Horses Author: Irene Nocera, Francesca Bonelli, Valentina Vitale, Valentina Meucci, Giuseppe Conte, Eduard Jose-Cunilleras, Luis Alfonso Gracia-Calvo and Micaela Sgorbini Subject: Colic horses show systemic inflammatory response syndrome (SIRS) clinical signs. Procalcitonin (PCT) showed increased circulating levels in sick horses. This study compares plasma PCT concentrations in healthy vs. SIRS negative/positive colic horses over time, and evaluates PCT and SIRS score potential correlation, to verify the usefulness of PCT for the evaluation of SIRS severity. Ninety-one horses were included; 43/91 were healthy, on basis of physical examination, blood work and SIRS score (score = 0), while 48/91 were sick colic horses, classified as SIRS-negative (score < 2) and positive (score 2). Moreover, a 0 6 point-scale SIRS score was calculated (assessing mucous membrane color and blood lactate concentration). PCT was evaluated at admission, and at 24, 48, 72 and 96 h, using a commercial kit for equine species. We verified by the ANOVA test PCT differences between healthy vs. colic horses, healthy vs. SIRS-negative or SIRS-positive colic horses, at all sampling times, and the correlation between the SIRS score at admission with the SIRS score. Statistically significant differences were detected between healthy vs. all colic horses and between healthy vs. SIRS-positive or negative horses at all sampling times. No correlation was observed between the SIRS score at admission and PCT values. PCT was statistically higher in colic horses compared to the healthy ones, suggesting a role as a biomarker for colic. 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