c891d5aa8549bb77131f26ef9a5bf6b1 pmc_34359190.pdf 289a8c6a7856aa759c26908a3185d8973fdb7b91 pmc_34359190.pdf 5d053221dab24bec8a55d6f84cf37fd1aefe60334f228a81e79d915572f221b7 pmc_34359190.pdf Title: Prenatal Exposure to Innately Preferred D-Limonene and Trans-Anethole Does Not Overcome Innate Aversion to Eucalyptol, Affecting Growth Performance of Weanling Piglets Subject: In the present research, two studies were performed to determine the effects of specific botanical compounds (BCs) on the innate feed preference and feed intake of piglets, as follows: Exp. 1 studied the innate feed preferences of post-weaning piglets using a double-choice feeding test. A total of 828 weaned piglets were distributed into 36 pens (23 pigs/pen) and assigned to three dietary pair choice feeding options (n = 12): unsupplemented prestarter diets (reference) versus reference plus D-limonene, trans-anethole, or eucalyptol. Piglets showed a preference for diets with D-limonene (53.8%) and trans-anethole (54.5%), and an aversion to eucalyptol (41.6%) (p < 0.05). Exp. 2 studied whether the prenatal and perinatal exposure to D-limonene, trans-anethole, and eucalyptol influences the feed intake and growth of newly-weaned piglets. Twenty-eight gestating and lactating sows were distributed into two dietary treatments (n = 14): unsupplemented Control diets or Control plus a blend of BCs (BBC; containing D-limonene, trans-anethole, and eucalyptol). D-limonene, trans-anethole, and eucalyptol were transferred into the placental fluid, and D-limonene and trans-anethole into the milk (p < 0.05). Furthermore, weanling piglets (n = 200; Control) and (n = 203; BBC) received the same treatment as their mothers in prestarter diets. The early response after weaning showed that piglets’ post-weaning BW gain was higher in the Control (p < 0.05) group than in those exposed to BBC. In conclusion, prenatal exposure to preferred D-limonene and trans-anethole, or familiarity to eucalyptol did not help to overcome the innate aversion to eucalyptol and its negative effect on weanling piglets’ BW. Keywords: botanical compounds; weanling piglets; innate feed preference; sensory maternallearning; growth performance; maternal transfer; hyperprolific sows Author: David Reyes-Camacho, José F. Pérez, Ester Vinyeta, Tobias Aumiller, Jan D. Van der Klis and David Solà-Oriol Creator: LaTeX with hyperref Producer: pdfTeX-1.40.21 CreationDate: Sat Jul 10 11:13:28 2021 CEST ModDate: Sat Jul 10 11:26:45 2021 CEST Custom Metadata: no Metadata Stream: no Tagged: no UserProperties: no Suspects: no Form: none JavaScript: no Pages: 12 Encrypted: no Page size: 595.276 x 841.89 pts (A4) Page rot: 0 File size: 1007259 bytes Optimized: no PDF version: 1.7 name type encoding emb sub uni object ID ------------------------------------ ----------------- ---------------- --- --- --- --------- MQYZDD+URWPalladioL-Ital Type 1 Custom yes yes no 10 0 ROEUNT+URWPalladioL-Bold Type 1 Custom yes yes no 15 0 PESSHF+URWPalladioL-BoldItal Type 1 Custom yes yes no 19 0 ANENSI+URWPalladioL-Roma Type 1 Custom yes yes no 23 0 MWJZGO+VnURWPalladioL-Bold Type 1 Custom yes yes no 27 0 VJAUGW+VnURWPalladioL Type 1 Custom yes yes no 32 0 SZMRXJ+TimesNewRomanPS-BoldItalicMT Type 1C WinAnsi yes yes no 40 0 MCBCCI+SourceSansRoman-Regular Type 1C Custom yes yes no 62 0 MCBCCM+SourceSansRoman.613wght Type 1C Custom yes yes no 66 0 MCBCCO+SourceSansRoman-Bold Type 1C Custom yes yes no 70 0 XEYJZU+CMSY10 Type 1 Builtin yes yes no 82 0 VVSYYB+EURM10 Type 1 Builtin yes yes no 92 0 MDBHJF+PalatinoLinotype,Bold TrueType WinAnsi yes yes no 115 0 MDBHJH+PalatinoLinotype TrueType WinAnsi yes yes no 118 0 MDBHJJ+PalatinoLinotype,Italic TrueType WinAnsi yes yes no 121 0 MDBHJF+PalatinoLinotype,Bold TrueType WinAnsi yes yes no 133 0 MDBHJH+PalatinoLinotype TrueType WinAnsi yes yes no 136 0 MDBHJJ+PalatinoLinotype,Italic TrueType WinAnsi yes yes no 139 0 NUUXUW+VnURWPalladioL-Italic Type 1 Custom yes yes no 150 0 Jhove (Rel. 1.28.0, 2023-05-18) Date: 2024-10-02 03:08:20 CEST RepresentationInformation: pmc_34359190.pdf ReportingModule: PDF-hul, Rel. 1.12.4 (2023-03-16) LastModified: 2023-03-31 12:14:45 CEST Size: 1007259 Format: PDF Version: 1.7 Status: Well-Formed and valid SignatureMatches: PDF-hul MIMEtype: application/pdf PDFMetadata: Objects: 297 FreeObjects: 1 IncrementalUpdates: 0 DocumentCatalog: PageLayout: SinglePage PageMode: UseNone Outlines: Item: Title: Introduction Destination: section.1 Item: Title: Materials and Methods Destination: section.2 Children: Item: Title: Exp. 1 (Double-Choice Feeding Test) Destination: subsection.2.1 Children: Item: Title: Experimental Design, Animals, and Housing Destination: subsubsection.2.1.1 Item: Title: Feeding Programme and Dietary Treatments Destination: subsubsection.2.1.2 Item: Title: Experimental Procedures, Data, and Sample Collection Destination: subsubsection.2.1.3 Item: Title: Statistical Analysis Destination: subsubsection.2.1.4 Item: Title: Exp. 2 (Sensory Maternal Learning) Destination: subsection.2.2 Children: Item: Title: Experimental Design, Animals, and Housing Destination: subsubsection.2.2.1 Item: Title: Feeding Programme and Dietary Treatments Destination: subsubsection.2.2.2 Item: Title: Experimental Procedures, Data and Sample Collection Destination: subsubsection.2.2.3 Item: Title: Statistical Analysis Destination: subsubsection.2.2.4 Item: Title: Results Destination: section.3 Children: Item: Title: Exp. 1 (Double-Choice Feeding Test) Destination: subsection.3.1 Children: Item: Title: Innate Feed Preference Destination: subsubsection.3.1.1 Item: Title: Piglet’s Growth Performance Destination: subsubsection.3.1.2 Item: Title: Exp. 2 (Sensory Maternal Learning) Destination: subsection.3.2 Children: Item: Title: Placental and Milk Maternal Transfer Destination: subsubsection.3.2.1 Item: Title: Piglet’s Growth Performance Destination: subsubsection.3.2.2 Item: Title: Discussion Destination: section.4 Children: Item: Title: Innate Preference or Aversive Responses of Weaning Piglets to BCs Destination: subsection.4.1 Item: Title: Effects of Prenatal Exposure to BBC on Weaning Piglet’s Growth Performance Destination: subsection.4.2 Item: Title: References Destination: subsection.4.2 Info: Title: Prenatal Exposure to Innately Preferred D-Limonene and Trans-Anethole Does Not Overcome Innate Aversion to Eucalyptol, Affecting Growth Performance of Weanling Piglets Author: David Reyes-Camacho, José F. Pérez, Ester Vinyeta, Tobias Aumiller, Jan D. Van der Klis and David Solà-Oriol Subject: In the present research, two studies were performed to determine the effects of specific botanical compounds (BCs) on the innate feed preference and feed intake of piglets, as follows: Exp. 1 studied the innate feed preferences of post-weaning piglets using a double-choice feeding test. A total of 828 weaned piglets were distributed into 36 pens (23 pigs/pen) and assigned to three dietary pair choice feeding options (n = 12): unsupplemented prestarter diets (reference) versus reference plus D-limonene, trans-anethole, or eucalyptol. Piglets showed a preference for diets with D-limonene (53.8%) and trans-anethole (54.5%), and an aversion to eucalyptol (41.6%) (p < 0.05). Exp. 2 studied whether the prenatal and perinatal exposure to D-limonene, trans-anethole, and eucalyptol influences the feed intake and growth of newly-weaned piglets. Twenty-eight gestating and lactating sows were distributed into two dietary treatments (n = 14): unsupplemented Control diets or Control plus a blend of BCs (BBC; containing D-limonene, trans-anethole, and eucalyptol). D-limonene, trans-anethole, and eucalyptol were transferred into the placental fluid, and D-limonene and trans-anethole into the milk (p < 0.05). Furthermore, weanling piglets (n = 200; Control) and (n = 203; BBC) received the same treatment as their mothers in prestarter diets. The early response after weaning showed that piglets’ post-weaning BW gain was higher in the Control (p < 0.05) group than in those exposed to BBC. In conclusion, prenatal exposure to preferred D-limonene and trans-anethole, or familiarity to eucalyptol did not help to overcome the innate aversion to eucalyptol and its negative effect on weanling piglets’ BW. 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