d5750145f74b0cd78539ab0e533187ef pmc_34436412.pdf 062c6a41b131fec7172718e10effdc7c48f898a6 pmc_34436412.pdf e6ef6756b79b64d6233277c32266935741c9318b30cf40e56b48e802e7d0dbf7 pmc_34436412.pdf Title: Chronic Effect of a Cafeteria Diet and Intensity of Resistance Training on the Circulating Lysophospholipidome in Young Rats Subject: The daily practice of physical exercise and a balanced diet are recommended to prevent metabolic syndrome (MetS). As MetS is a multifactorial disorder associated with the development of serious diseases, the advancement of comprehensive biomarkers could aid in an accurate diagnosis. In this regard, it is known that gut microbiota is altered in MetS, and especially, lipid metabolites species are highly modified, thus emerging as potential biomarkers. In preliminary studies, we observed that alterations in serum lysoglycerophospholipids (Lyso-PLs) were shared between animals with diet-induced MetS and those performing resistance exercises assiduously. Therefore, our objective was the targeted determination of the lysophospholipidome in young rats fed a standard (ST) or a cafeteria diet (CAF) and submitted to different training intensities to evaluate its potential as a biomarker of a detrimental lifestyle. Targeted metabolomics focused on lysophosphatidylcholines (Lyso-PCs) and lysophosphatidylethanolamines (Lyso-PEs) and multivariate statistics were used to achieve an integral understanding. Chronic intake of CAF altered the serological levels of both lipid subclasses. Twenty-two Lyso-PLs were significantly altered by CAF, from which we selected Lyso-PCs (14:0), (17:1) and (20:2) and Lyso-PEs (18:2) and (18:3) as they were enough to achieve an optimal prediction. The main effect of physical training was decreased Lyso-PEs levels with disparities among training intensities for each diet. We concluded that an examination of the lysophospholipidome reveals the general state of the metabolome in young female rats, especially due to intake of an MetS-inducing diet, thus highlighting the importance of this family of compounds in lipid disorders. Keywords: lysoglycerophospholipids; metabolic syndrome; cafeteria diet; resistance training Author: Susana Suárez-García, Antoni Caimari, Josep M. del Bas, Jaume Lalanza, Rosa M. Escorihuela, Manuel Suárez, Cristina Torres-Fuentes and Lluís Arola Creator: LaTeX with hyperref Producer: pdfTeX-1.40.21 CreationDate: Mon Jul 26 08:22:31 2021 CEST ModDate: Mon Jul 26 08:49:00 2021 CEST Custom Metadata: no Metadata Stream: no Tagged: no UserProperties: no Suspects: no Form: none JavaScript: no Pages: 13 Encrypted: no Page size: 595.276 x 841.89 pts (A4) Page rot: 0 File size: 1689128 bytes Optimized: no PDF version: 1.7 name type encoding emb sub uni object ID ------------------------------------ ----------------- ---------------- --- --- --- --------- EUELZI+VnURWPalladioL Type 1 Custom yes yes no 10 0 PSLBYC+URWPalladioL-Ital Type 1 Custom yes yes no 15 0 IPHTHY+URWPalladioL-Bold Type 1 Custom yes yes no 20 0 EPUTCT+URWPalladioL-Roma Type 1 Custom yes yes no 24 0 SSNIUI+VnURWPalladioL-Bold Type 1 Custom yes yes no 28 0 ZZPUEX+ArialMT Type 1C WinAnsi yes yes no 37 0 TWGUDY+TimesNewRomanPS-BoldItalicMT Type 1C WinAnsi yes yes no 40 0 MCBCCI+SourceSansRoman-Regular Type 1C Custom yes yes no 62 0 MCBCCM+SourceSansRoman.613wght Type 1C Custom yes yes no 66 0 MCBCCO+SourceSansRoman-Bold Type 1C Custom yes yes no 70 0 ZXDKIP+EURM10 Type 1 Builtin yes yes no 82 0 XIRVNA+EURB10 Type 1 Builtin yes yes no 89 0 QMDDXF+CMSY10 Type 1 Builtin yes yes no 93 0 BCDEEE+PalatinoLinotype-Bold TrueType WinAnsi yes yes no 105 0 BCDFEE+PalatinoLinotype-Roman TrueType WinAnsi yes yes no 109 0 BCDGEE+PalatinoLinotype-Italic TrueType WinAnsi yes yes no 113 0 ArialMT TrueType WinAnsi no no no 120 0 BCDEEE+PalatinoLinotype-Bold TrueType WinAnsi yes yes no 131 0 BCDFEE+PalatinoLinotype-Roman TrueType WinAnsi yes yes no 135 0 BCDGEE+PalatinoLinotype-Italic TrueType WinAnsi yes yes no 139 0 BCDIEE+PalatinoLinotype-Roman CID TrueType Identity-H yes yes yes 143 0 ArialMT TrueType WinAnsi no no no 152 0 Arial-BoldMT TrueType WinAnsi no no no 155 0 Arial-ItalicMT TrueType WinAnsi no no no 158 0 Arial-BoldItalicMT TrueType WinAnsi no no no 161 0 BCDEEE+PalatinoLinotype-Bold TrueType WinAnsi yes yes no 190 0 BCDFEE+PalatinoLinotype-Roman TrueType WinAnsi yes yes no 194 0 BCDGEE+PalatinoLinotype-Italic TrueType WinAnsi yes yes no 198 0 BCDIEE+PalatinoLinotype-Roman CID TrueType Identity-H yes yes yes 202 0 ArialMT TrueType WinAnsi no no no 211 0 Arial-BoldMT TrueType WinAnsi no no no 214 0 Arial-ItalicMT TrueType WinAnsi no no no 217 0 Arial-BoldItalicMT TrueType WinAnsi no no no 220 0 BCDEEE+PalatinoLinotype-Bold TrueType WinAnsi yes yes no 246 0 BCDFEE+PalatinoLinotype-Roman TrueType WinAnsi yes yes no 250 0 BCDGEE+PalatinoLinotype-Italic TrueType WinAnsi yes yes no 254 0 BCDIEE+PalatinoLinotype-Roman CID TrueType Identity-H yes yes yes 258 0 Arial-BoldMT TrueType WinAnsi no no no 266 0 ArialMT CID TrueType Identity-H yes no yes 270 0 ArialMT TrueType WinAnsi no no no 278 0 Jhove (Rel. 1.28.0, 2023-05-18) Date: 2024-10-02 03:06:28 CEST RepresentationInformation: pmc_34436412.pdf ReportingModule: PDF-hul, Rel. 1.12.4 (2023-03-16) LastModified: 2023-03-31 12:14:53 CEST Size: 1689128 Format: PDF Version: 1.7 Status: Well-Formed and valid SignatureMatches: PDF-hul MIMEtype: application/pdf PDFMetadata: Objects: 464 FreeObjects: 1 IncrementalUpdates: 0 DocumentCatalog: PageLayout: SinglePage PageMode: UseNone Outlines: Item: Title: Introduction Destination: section.1 Item: Title: Results Destination: section.2 Children: Item: Title: Determination of the Circulating Levels of Lyso-PLs Destination: subsection.2.1 Item: Title: Multivariate Statistics Reveals Lyso-PLs Alteration Association with Cafeteria Diet Destination: subsection.2.2 Item: Title: Lyso-PLs as Poor Indicators of the Practice of Daily Exercise Destination: subsection.2.3 Item: Title: Discussion Destination: section.3 Item: Title: Materials and Methods Destination: section.4 Children: Item: Title: Ethics Statement Destination: subsection.4.1 Item: Title: Animals Destination: subsection.4.2 Item: Title: Chemicals Destination: subsection.4.3 Item: Title: Preparation of Standard Curves Destination: subsection.4.4 Item: Title: Sample Processing Destination: subsection.4.5 Item: Title: Targeted Metabolomics Analysis of Lyso-PLs Destination: subsection.4.6 Item: Title: Statistical Analyses Destination: subsection.4.7 Children: Item: Title: Univariate Analysis Destination: subsubsection.4.7.1 Item: Title: Multivariate Analysis Destination: subsubsection.4.7.2 Item: Title: References Destination: subsubsection.4.7.2 Info: Title: Chronic Effect of a Cafeteria Diet and Intensity of Resistance Training on the Circulating Lysophospholipidome in Young Rats Author: Susana Suárez-García, Antoni Caimari, Josep M. del Bas, Jaume Lalanza, Rosa M. Escorihuela, Manuel Suárez, Cristina Torres-Fuentes and Lluís Arola Subject: The daily practice of physical exercise and a balanced diet are recommended to prevent metabolic syndrome (MetS). As MetS is a multifactorial disorder associated with the development of serious diseases, the advancement of comprehensive biomarkers could aid in an accurate diagnosis. In this regard, it is known that gut microbiota is altered in MetS, and especially, lipid metabolites species are highly modified, thus emerging as potential biomarkers. In preliminary studies, we observed that alterations in serum lysoglycerophospholipids (Lyso-PLs) were shared between animals with diet-induced MetS and those performing resistance exercises assiduously. Therefore, our objective was the targeted determination of the lysophospholipidome in young rats fed a standard (ST) or a cafeteria diet (CAF) and submitted to different training intensities to evaluate its potential as a biomarker of a detrimental lifestyle. Targeted metabolomics focused on lysophosphatidylcholines (Lyso-PCs) and lysophosphatidylethanolamines (Lyso-PEs) and multivariate statistics were used to achieve an integral understanding. Chronic intake of CAF altered the serological levels of both lipid subclasses. Twenty-two Lyso-PLs were significantly altered by CAF, from which we selected Lyso-PCs (14:0), (17:1) and (20:2) and Lyso-PEs (18:2) and (18:3) as they were enough to achieve an optimal prediction. The main effect of physical training was decreased Lyso-PEs levels with disparities among training intensities for each diet. We concluded that an examination of the lysophospholipidome reveals the general state of the metabolome in young female rats, especially due to intake of an MetS-inducing diet, thus highlighting the importance of this family of compounds in lipid disorders. Keywords: lysoglycerophospholipids; metabolic syndrome; cafeteria diet; resistance training Creator: LaTeX with hyperref Producer: pdfTeX-1.40.21 CreationDate: Mon Jul 26 08:22:31 CEST 2021 ModDate: Mon Jul 26 08:49:00 CEST 2021 ID: 0x27ff239d830741b6fc06cc79081fa8a9, 0x27ff239d830741b6fc06cc79081fa8a9 Filters: FilterPipeline: FlateDecode Fonts: Type1: Font: BaseFont: ZXDKIP+EURM10 FontSubset: true FirstChar: 11 LastChar: 22 FontDescriptor: FontName: ZXDKIP+EURM10 Flags: Symbolic FontBBox: -32, -243, 1060, 720 FontFile: true Font: BaseFont: IPHTHY+URWPalladioL-Bold FontSubset: true FirstChar: 3 LastChar: 122 FontDescriptor: FontName: IPHTHY+URWPalladioL-Bold Flags: Symbolic FontBBox: -152, -301, 1000, 935 FontFile: true EncodingDictionary: Differences: true Font: BaseFont: EPUTCT+URWPalladioL-Roma FontSubset: true FirstChar: 2 LastChar: 246 FontDescriptor: FontName: EPUTCT+URWPalladioL-Roma Flags: Symbolic FontBBox: -166, -283, 1021, 943 FontFile: true EncodingDictionary: Differences: 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