4256ae784a7a7c5bb8ff31f17aff88ab pmc_34438709.pdf 34cde91cc6e95a59431135f813140127a0c17899 pmc_34438709.pdf 9dada2a889401779f566ceba342b40beca17191acbdaf709acc89d6278e4e5ec pmc_34438709.pdf Title: Short Communication: Quantification of the Effect of Mycotoxin Binders on the Bioavailability of Fat-Soluble Vitamins In Vitro Subject: The aim of this study was to determine the capacity of six mycotoxin binders (MTBs) to adsorb vitamins A, D and E in an in vitro system that simulates gastric and intestinal digestion. Experiment 1 evaluated the recovery rate of vitamins A, D and E in the incubation conditions. In Experiment 2, the main factors were the MTB (bentonite, clinoptilolite, sepiolite, montmorillonite, active carbon and yeast cell walls), vitamins (A, D and E) and incubation type (vitamins incubated separately or together). The recovery was high for vitamin D (83%) and E (93%), but low for vitamin A (23%), for which no further analyses were conducted. When incubated separately, vitamin D was only adsorbed by yeast cell wall (20.2%). Vitamin E adsorption was highest with bentonite (54.5%) and montmorillonite (46.3%) and lowest with sepiolite (16.6%) and active carbon (18.5%). When incubated together, vitamin D was not adsorbed by any MTB. Vitamin E adsorption was highest in bentonite (61.8%) and montmorillonite (50.7%) and lowest in sepiolite (15.4%). Results indicate that the bioavailability of vitamin E, but not that of vitamin D, may be reduced in the presence of MTBs. Keywords: mycotoxin binder; adsorption; fat-soluble vitamins Author: Abdelhacib Kihal, María Ercilda Rodríguez-Prado, Carles Cristofol and Sergio Calsamiglia Creator: LaTeX with hyperref Producer: pdfTeX-1.40.21 CreationDate: Fri Jul 30 11:21:56 2021 CEST ModDate: Fri Jul 30 11:26:34 2021 CEST Custom Metadata: no Metadata Stream: no Tagged: no UserProperties: no Suspects: no Form: none JavaScript: no Pages: 6 Encrypted: no Page size: 595.276 x 841.89 pts (A4) Page rot: 0 File size: 583710 bytes Optimized: no PDF version: 1.7 name type encoding emb sub uni object ID ------------------------------------ ----------------- ---------------- --- --- --- --------- IODWWR+VnURWPalladioL Type 1 Custom yes yes no 10 0 QIWEZF+URWPalladioL-Ital Type 1 Custom yes yes no 15 0 KYJMDM+URWPalladioL-Bold Type 1 Custom yes yes no 20 0 PWMDCT+URWPalladioL-Roma Type 1 Custom yes yes no 24 0 WLENKM+VnURWPalladioL-Bold Type 1 Custom yes yes no 28 0 SZMRXJ+TimesNewRomanPS-BoldItalicMT Type 1C WinAnsi yes yes no 36 0 MCBCCI+SourceSansRoman-Regular Type 1C Custom yes yes no 58 0 MCBCCM+SourceSansRoman.613wght Type 1C Custom yes yes no 62 0 MCBCCO+SourceSansRoman-Bold Type 1C Custom yes yes no 66 0 VORBJF+CMSY10 Type 1 Builtin yes yes no 78 0 VVSYYB+EURM10 Type 1 Builtin yes yes no 82 0 NKUZIX+MSAM10 Type 1 Builtin yes yes no 93 0 BCDEEE+PalatinoLinotype-Bold TrueType WinAnsi yes yes no 101 0 BCDFEE+PalatinoLinotype-Roman TrueType WinAnsi yes yes no 105 0 BCDGEE+PalatinoLinotype-Italic TrueType WinAnsi yes yes no 109 0 BCDHEE+PalatinoLinotype-Roman CID TrueType Identity-H yes yes yes 113 0 BCDJEE+SegoeUIEmoji CID TrueType Identity-H yes yes yes 120 0 TimesNewRomanPSMT CID TrueType Identity-H yes no yes 128 0 Jhove (Rel. 1.28.0, 2023-05-18) Date: 2024-10-02 03:06:24 CEST RepresentationInformation: pmc_34438709.pdf ReportingModule: PDF-hul, Rel. 1.12.4 (2023-03-16) LastModified: 2023-03-31 12:14:53 CEST Size: 583710 Format: PDF Version: 1.7 Status: Well-Formed and valid SignatureMatches: PDF-hul MIMEtype: application/pdf PDFMetadata: Objects: 203 FreeObjects: 1 IncrementalUpdates: 0 DocumentCatalog: PageLayout: SinglePage PageMode: UseNone Outlines: Item: Title: Introduction Destination: section.1 Item: Title: Materials and Methods Destination: section.2 Children: Item: Title: Experimental Design and Incubations Destination: subsection.2.1 Item: Title: Calculations and Statistical Analysis Destination: subsection.2.2 Item: Title: Results Destination: section.3 Item: Title: Discussion Destination: section.4 Item: Title: Conclusions Destination: section.5 Item: Title: References Destination: section.5 Info: Title: Short Communication: Quantification of the Effect of Mycotoxin Binders on the Bioavailability of Fat-Soluble Vitamins In Vitro Author: Abdelhacib Kihal, María Ercilda Rodríguez-Prado, Carles Cristofol and Sergio Calsamiglia Subject: The aim of this study was to determine the capacity of six mycotoxin binders (MTBs) to adsorb vitamins A, D and E in an in vitro system that simulates gastric and intestinal digestion. Experiment 1 evaluated the recovery rate of vitamins A, D and E in the incubation conditions. In Experiment 2, the main factors were the MTB (bentonite, clinoptilolite, sepiolite, montmorillonite, active carbon and yeast cell walls), vitamins (A, D and E) and incubation type (vitamins incubated separately or together). The recovery was high for vitamin D (83%) and E (93%), but low for vitamin A (23%), for which no further analyses were conducted. When incubated separately, vitamin D was only adsorbed by yeast cell wall (20.2%). Vitamin E adsorption was highest with bentonite (54.5%) and montmorillonite (46.3%) and lowest with sepiolite (16.6%) and active carbon (18.5%). When incubated together, vitamin D was not adsorbed by any MTB. Vitamin E adsorption was highest in bentonite (61.8%) and montmorillonite (50.7%) and lowest in sepiolite (15.4%). Results indicate that the bioavailability of vitamin E, but not that of vitamin D, may be reduced in the presence of MTBs. 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