1539b830df0ac31c0249fe66c968214e pmc_34439450.pdf 7903403938e89d3247357db33d5831eaf59f1397 pmc_34439450.pdf 9165b5a5d01a0839b625fdaf8d4eddf3314ce849313638f87a262c406418db4c pmc_34439450.pdf Title: Measurement of Oxidative Stress Index in Seminal Plasma Can Predict In Vivo Fertility of Liquid-Stored Porcine Artificial Insemination Semen Doses Subject: The study evaluated the relation between the oxidative stress index (OSI) in porcine seminal plasma (n = 76) with sperm resilience and in vivo fertility (farrowing rate and litter size of 3137 inseminated sows) of liquid-stored artificial insemination (AI) semen doses. The OSI was assessed as the ratio of advanced oxidation protein products to Trolox-equivalent antioxidant capacity, both measured using an automated analyzer. Sperm motility (computer-assisted sperm analyzer) and viability (flow cytometry) were evaluated in semen AI-doses at 0 and 72 h of storage at 17 C. Sperm resilience was defined as the difference between storage intervals. Semen AI-doses were hierarchically clustered as having high, medium and low seminal OSI (p < 0.001) with those of low displaying higher resilience (p < 0.01). Boars were hierarchically clustered into two groups(p < 0.001) as having either positive or negative farrowing rate and litter size deviation; the negative one showing higher seminal OSI (p < 0.05). In sum, seminal OSI was negatively related to sperm motility and the in vivo fertility of liquid-stored boar semen AI-doses, with the receiver operating characteristic curve presenting seminal OSI as a good predictive biomarker of in vivo fertility of AI-boars (area under the curve: 0.815, p < 0.05). Keywords: in vivo fertility; oxidative stress index; pig; seminal plasma; sperm quality Author: Isabel Barranco, Camila P. Rubio, Asta Tvarijonaviciute, Heriberto Rodriguez-Martinez and Jordi Roca Creator: LaTeX with hyperref Producer: pdfTeX-1.40.21 CreationDate: Thu Aug 19 16:03:33 2021 CEST ModDate: Thu Aug 19 16:07:02 2021 CEST Tagged: no UserProperties: no Suspects: no Form: none JavaScript: no Pages: 12 Encrypted: no Page size: 595.276 x 841.89 pts (A4) Page rot: 0 File size: 1436347 bytes Optimized: no PDF version: 1.7 name type encoding emb sub uni object ID ------------------------------------ ----------------- ---------------- --- --- --- --------- IEFSKB+VnURWPalladioL Type 1 Custom yes yes no 10 0 FTEZOE+URWPalladioL-Ital Type 1 Custom yes yes no 15 0 FCAKNA+URWPalladioL-Bold Type 1 Custom yes yes no 20 0 HHSFMQ+URWPalladioL-Roma Type 1 Custom yes yes no 24 0 PDYDVH+CMSY10 Type 1 Builtin yes yes no 28 0 KUDXZB+TimesNewRomanPS-BoldItalicMT Type 1C WinAnsi yes yes no 36 0 MCBCCI+SourceSansRoman-Regular Type 1C Custom yes yes no 58 0 MCBCCM+SourceSansRoman.613wght Type 1C Custom yes yes no 62 0 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LMHDHN+PalatinoLinotype TrueType WinAnsi yes yes no 183 0 LMHDHP+PalatinoLinotype,Italic TrueType WinAnsi yes yes no 186 0 LMHDGM+PalatinoLinotype,Bold TrueType WinAnsi yes yes no 201 0 LMHDGN+PalatinoLinotype CID TrueType Identity-H yes yes yes 204 0 LMHDHN+PalatinoLinotype TrueType WinAnsi yes yes no 210 0 LMHDHP+PalatinoLinotype,Italic TrueType WinAnsi yes yes no 213 0 Jhove (Rel. 1.22.1, 2019-04-17) Date: 2023-10-21 02:36:30 CEST RepresentationInformation: pmc_34439450.pdf ReportingModule: PDF-hul, Rel. 1.12.1 (2019-04-17) LastModified: 2023-03-31 12:14:54 CEST Size: 1436347 Format: PDF Version: 1.7 Status: Well-Formed and valid SignatureMatches: PDF-hul MIMEtype: application/pdf PDFMetadata: Objects: 407 FreeObjects: 1 IncrementalUpdates: 0 DocumentCatalog: PageLayout: SinglePage PageMode: UseNone Outlines: Item: Title: Introduction Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@262b2c86 Item: Title: Materials and Methods Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@371a67ec Children: Item: Title: Reagents and Media Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@5ed828d Item: Title: Boars, Ejaculates and Seminal Plasma Samples Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@50d0686 Item: Title: Oxidative Stress Index Measurement in SP Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@7a3d45bd Item: Title: Assessment of Sperm Quality Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@1e7c7811 Item: Title: Experimental Design Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@77ec78b9 Children: Item: Title: Experiment 1: Relationship between Seminal OSI and the Sperm Quality of Semen AI-Doses Stored at 17 C for 72 h Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@1a3869f4 Item: Title: Experiment 2: Relationship between Seminal OSI and In Vivo Fertility of Semen AI-Doses Stored at 17 C Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@a38d7a3 Item: Title: Statistical Analysis Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@77f99a05 Item: Title: Results Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@63440df3 Children: Item: Title: Experiment 1: Relationship between Seminal OSI and Sperm Quality of Semen AI-Doses Stored at 17 C for 72 h Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@3aeaafa6 Item: Title: Experiment 2: Relationship between Seminal OSI and In Vivo Fertility of Semen AI-Doses Stored at 17 C Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@76a3e297 Item: Title: Discussion Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@4d3167f4 Item: Title: Conclusions Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@ed9d034 Item: Title: References Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@6121c9d6 Info: Title: Measurement of Oxidative Stress Index in Seminal Plasma Can Predict In Vivo Fertility of Liquid-Stored Porcine Artificial Insemination Semen Doses Author: Isabel Barranco, Camila P. Rubio, Asta Tvarijonaviciute, Heriberto Rodriguez-Martinez and Jordi Roca Subject: The study evaluated the relation between the oxidative stress index (OSI) in porcine seminal plasma (n = 76) with sperm resilience and in vivo fertility (farrowing rate and litter size of 3137 inseminated sows) of liquid-stored artificial insemination (AI) semen doses. The OSI was assessed as the ratio of advanced oxidation protein products to Trolox-equivalent antioxidant capacity, both measured using an automated analyzer. Sperm motility (computer-assisted sperm analyzer) and viability (flow cytometry) were evaluated in semen AI-doses at 0 and 72 h of storage at 17 C. Sperm resilience was defined as the difference between storage intervals. Semen AI-doses were hierarchically clustered as having high, medium and low seminal OSI (p < 0.001) with those of low displaying higher resilience (p < 0.01). Boars were hierarchically clustered into two groups(p < 0.001) as having either positive or negative farrowing rate and litter size deviation; the negative one showing higher seminal OSI (p < 0.05). In sum, seminal OSI was negatively related to sperm motility and the in vivo fertility of liquid-stored boar semen AI-doses, with the receiver operating characteristic curve presenting seminal OSI as a good predictive biomarker of in vivo fertility of AI-boars (area under the curve: 0.815, p < 0.05). 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