952f1b0da4c9ce3fe2569df3ffb9d33f  pmc_34440283.pdf
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4225a3f4a0efe671429bbd5046fc5f40f766b0e8f2b12851c1aa64156df2d55f  pmc_34440283.pdf

Title:          Maternal Phylogenetic Relationships and Genetic Variation among Rare, Phenotypically Similar Donkey Breeds
Subject:        The mitochondrial DNA (mtDNA) D-loop of endangered and critically endangered breeds has been studied to identify maternal lineages, characterize genetic inheritance, reconstruct phylogenetic relations among breeds, and develop biodiversity conservation and breeding programs. The aim of the study was to determine the variability remaining and the phylogenetic relationship of Martina Franca (MF, with total population of 160 females and 36 males), Ragusano (RG, 344 females and 30 males), Pantesco (PT, 47 females and 15 males), and Catalonian (CT) donkeys by collecting genetic data from maternal lineages. Genetic material was collected from saliva, and a 350 bp fragment of D-loop mtDNA was amplified and sequenced. Sequences were aligned and evaluated using standard bioinformatics software. A total of 56 haplotypes including 33 polymorphic sites were found in 77 samples (27 MF, 22 RG, 8 PT, 19 CT, 1 crossbred). The breed nucleotide diversity value () for all the breeds was 0.128 (MF: 0.162, RG: 0.132, PT: 0.025, CT: 0.038). Principal components analysis grouped most of the haplogroups into two different clusters, I (including all haplotypes from PT and CT, together with haplotypes from MF and RG) and II (including haplotypes from MF and RG only). In conclusion, we found that the primeval haplotypes, haplogroup variability, and a large number of maternal lineages were preserved in MF and RG; thus, these breeds play putative pivotal roles in the phyletic relationships of donkey breeds. Maternal inheritance is indispensable genetic information required to evaluate inheritance, variability, and breeding programs.
Keywords:       genetics; mitochondrial DNA; donkey; Martina Franca; Ragusano; Pantesco; Catalonian
Author:         Andrea Mazzatenta, Massimo Vignoli, Maurizio Caputo, Giorgio Vignola, Roberto Tamburro, Francesco De Sanctis, Jordi Mirò Roig, Roberta Bucci, Domenico Robbe and Augusto Carluccio
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      Title: Materials and Methods 
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     Item: 
      Title: Results 
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       Item: 
        Title: Breed Haplotype Analysis 
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   Info: 
    Title: Maternal Phylogenetic Relationships and Genetic Variation among Rare, Phenotypically Similar Donkey Breeds
    Author: Andrea Mazzatenta, Massimo Vignoli, Maurizio Caputo, Giorgio Vignola, Roberto Tamburro, Francesco De Sanctis, Jordi Mirò Roig, Roberta Bucci, Domenico Robbe and Augusto Carluccio
    Subject: The mitochondrial DNA (mtDNA) D-loop of endangered and critically endangered breeds has been studied to identify maternal lineages, characterize genetic inheritance, reconstruct phylogenetic relations among breeds, and develop biodiversity conservation and breeding programs. The aim of the study was to determine the variability remaining and the phylogenetic relationship of Martina Franca (MF, with total population of 160 females and 36 males), Ragusano (RG, 344 females and 30 males), Pantesco (PT, 47 females and 15 males), and Catalonian (CT) donkeys by collecting genetic data from maternal lineages. Genetic material was collected from saliva, and a 350 bp fragment of D-loop mtDNA was amplified and sequenced. Sequences were aligned and evaluated using standard bioinformatics software. A total of 56 haplotypes including 33 polymorphic sites were found in 77 samples (27 MF, 22 RG, 8 PT, 19 CT, 1 crossbred). The breed nucleotide diversity value () for all the breeds was 0.128 (MF: 0.162, RG: 0.132, PT: 0.025, CT: 0.038). Principal components analysis grouped most of the haplogroups into two different clusters, I (including all haplotypes from PT and CT, together with haplotypes from MF and RG) and II (including haplotypes from MF and RG only). In conclusion, we found that the primeval haplotypes, haplogroup variability, and a large number of maternal lineages were preserved in MF and RG; thus, these breeds play putative pivotal roles in the phyletic relationships of donkey breeds. Maternal inheritance is indispensable genetic information required to evaluate inheritance, variability, and breeding programs.
    Keywords: genetics; mitochondrial DNA; donkey; Martina Franca; Ragusano; Pantesco; Catalonian
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   Type: CRC32
  Checksum: 952f1b0da4c9ce3fe2569df3ffb9d33f
   Type: MD5
  Checksum: 324bff23e3b69fe488cac7def9e32c7c82834a89
   Type: SHA-1