e7e4f4a84544d4c897d27bba65bbac70 pmc_34444713.pdf 7471533a5c7ba9dff594d507cec8a27b5e39c668 pmc_34444713.pdf a6450278d975bb612d850eae60b43c5a8a4155018e83b2f36d330f7388ceb57d pmc_34444713.pdf Title: Role of Dietary Nutritional Treatment on Hepatic and Intestinal Damage in Transplantation with Steatotic and Non-Steatotic Liver Grafts from Brain Dead Donors Subject: Herein, we investigate whether: (1) the administration of glucose or a lipid emulsion is useful in liver transplantation (LT) using steatotic (induced genetically or nutritionally) or non-steatotic livers from donors after brain death (DBDs); and (2) any such benefits are due to reductions in intestinal damage and consequently to gut microbiota preservation. In recipients from DBDs, we show increased hepatic damage and failure in the maintenance of ATP, glycogen, phospholipid and growth factor (HGF, IGF1 and VEGFA) levels, compared to recipients from non-DBDs. In recipients of non-steatotic grafts from DBDs, the administration of glucose or lipids did not protect against hepatic damage. This was associated with unchanged ATP, glycogen, phospholipid and growth factor levels. However, the administration of lipids in steatotic grafts from DBDs protected against damage and ATP and glycogen drop and increased phospholipid levels. This was associated with increases in growth factors. In all recipients from DBDs, intestinal inflammation and damage (evaluated by LPS, vascular permeability, mucosal damage, TLR4, TNF, IL1, IL-10, MPO, MDA and edema formation) was not shown. In such cases, potential changes in gut microbiota would not be relevant since neither inflammation nor damage was evidenced in the intestine following LT in any of the groups evaluated. In conclusion, lipid treatment is the preferable nutritional support to protect against hepatic damage in steatotic LT from DBDs; the benefits were independent of alterations in the recipient intestine. Keywords: brain death; liver transplantation; steatotic liver grafts; ischemia-reperfusion; polysaccharides; glucose; lipid emulsion; intestinal inflammation; gut microbiota Author: Marc Micó-Carnero, Araní Casillas-Ramírez, Albert Caballeria-Casals, Carlos Rojano-Alfonso, Alfredo Sánchez-González and Carmen Peralta Creator: LaTeX with hyperref Producer: pdfTeX-1.40.21 CreationDate: Mon Jul 26 16:43:08 2021 CEST ModDate: Mon Jul 26 16:48:59 2021 CEST Tagged: no UserProperties: no Suspects: no Form: none JavaScript: no Pages: 21 Encrypted: no Page size: 595.276 x 841.89 pts (A4) Page rot: 0 File size: 9249041 bytes Optimized: no PDF version: 1.7 name type encoding emb sub uni object ID ------------------------------------ ----------------- ---------------- --- --- --- --------- PJPSZX+VnURWPalladioL Type 1 Custom yes yes no 10 0 MBJRRL+URWPalladioL-Ital Type 1 Custom yes yes no 15 0 DURBYY+URWPalladioL-Bold Type 1 Custom yes yes no 20 0 QGMKND+URWPalladioL-Roma Type 1 Custom yes yes no 24 0 PRJKGQ+VnURWPalladioL-Bold Type 1 Custom yes yes no 28 0 HGMNRX+TimesNewRomanPS-BoldItalicMT Type 1C WinAnsi yes yes no 36 0 MCBCCI+SourceSansRoman-Regular Type 1C Custom yes yes no 58 0 MCBCCM+SourceSansRoman.613wght Type 1C Custom yes yes no 62 0 MCBCCO+SourceSansRoman-Bold Type 1C Custom yes yes no 66 0 VVSYYB+EURM10 Type 1 Builtin yes yes no 78 0 SFWFME+CMSY10 Type 1 Builtin yes yes no 88 0 ILKLDB+PalatinoLinotype,Bold TrueType WinAnsi yes yes no 105 0 ILKLDD+PalatinoLinotype TrueType WinAnsi yes yes no 108 0 ILKLDF+PalatinoLinotype,Italic TrueType WinAnsi yes yes no 111 0 Jhove (Rel. 1.22.1, 2019-04-17) Date: 2021-09-11 02:50:58 CEST RepresentationInformation: pmc_34444713.pdf ReportingModule: PDF-hul, Rel. 1.12.1 (2019-04-17) LastModified: 2021-08-30 03:34:43 CEST Size: 9249041 Format: PDF Version: 1.7 Status: Well-Formed and valid SignatureMatches: PDF-hul MIMEtype: application/pdf PDFMetadata: Objects: 418 FreeObjects: 1 IncrementalUpdates: 0 DocumentCatalog: PageLayout: SinglePage PageMode: UseNone Outlines: Item: Title: Introduction Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@13c27452 Item: Title: Materials and Methods Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@262b2c86 Children: Item: Title: Experimental Animals Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@371a67ec Item: Title: Experimental Design Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@5ed828d Item: Title: Biochemical Determinations Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@50d0686 Item: Title: Histology Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@7a3d45bd Item: Title: Statistics Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@1e7c7811 Item: Title: Results Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@77ec78b9 Children: Item: Title: Effects of Lipid and Glucose Administration on Hepatic Damage in Steatotic and Non-Steatotic LT in a Genetic Obesity Experimental Model Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@1a3869f4 Item: Title: Mechanisms Underlying Glucose and Lipid Action on Steatotic and Non-Steatotic Livers in a Genetic Obesity Experimental Model Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@a38d7a3 Item: Title: Role of Intestine in the Effects of Lipid and Glucose Administration on Hepatic Damage in Steatotic and Non-Steatotic LT from DBDs in a Genetic Obesity Experimental Model Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@77f99a05 Item: Title: Hepatic Damage and Intestinal Inflammation in LT from DBDs in a Nutritionally Induced Obesity Model Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@63440df3 Item: Title: Discussion Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@3aeaafa6 Item: Title: Conclusions Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@76a3e297 Item: Title: References Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@4d3167f4 Info: Title: Role of Dietary Nutritional Treatment on Hepatic and Intestinal Damage in Transplantation with Steatotic and Non-Steatotic Liver Grafts from Brain Dead Donors Author: Marc Micó-Carnero, Araní Casillas-Ramírez, Albert Caballeria-Casals, Carlos Rojano-Alfonso, Alfredo Sánchez-González and Carmen Peralta Subject: Herein, we investigate whether: (1) the administration of glucose or a lipid emulsion is useful in liver transplantation (LT) using steatotic (induced genetically or nutritionally) or non-steatotic livers from donors after brain death (DBDs); and (2) any such benefits are due to reductions in intestinal damage and consequently to gut microbiota preservation. In recipients from DBDs, we show increased hepatic damage and failure in the maintenance of ATP, glycogen, phospholipid and growth factor (HGF, IGF1 and VEGFA) levels, compared to recipients from non-DBDs. In recipients of non-steatotic grafts from DBDs, the administration of glucose or lipids did not protect against hepatic damage. This was associated with unchanged ATP, glycogen, phospholipid and growth factor levels. However, the administration of lipids in steatotic grafts from DBDs protected against damage and ATP and glycogen drop and increased phospholipid levels. This was associated with increases in growth factors. In all recipients from DBDs, intestinal inflammation and damage (evaluated by LPS, vascular permeability, mucosal damage, TLR4, TNF, IL1, IL-10, MPO, MDA and edema formation) was not shown. In such cases, potential changes in gut microbiota would not be relevant since neither inflammation nor damage was evidenced in the intestine following LT in any of the groups evaluated. In conclusion, lipid treatment is the preferable nutritional support to protect against hepatic damage in steatotic LT from DBDs; the benefits were independent of alterations in the recipient intestine. 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