fc400ec265745adfbf727711fe5dc372 antibiotics_11_00466_v2.pdf 4d76c903beffb8354331c0c81fb38faeaafc48e6 antibiotics_11_00466_v2.pdf 459f13b3356aa2b1bec8305535f657891a5f4ad31f7c3450b799d1581c4f4f4d antibiotics_11_00466_v2.pdf Title: A New Variant of the aadE-sat4-aphA-3 Gene Cluster Found in a Conjugative Plasmid from a MDR Campylobacter jejuni Isolate Subject: Campylobacter jejuni is a foodborne pathogen causing bacterial gastroenteritis, with the highest incidence reported in Europe. The prevalence of antibiotic resistance in C. jejuni, as well as in many other bacterial pathogens, has increased over the last few years. In this report, we describe the presence of a plasmid in a multi-drug-resistant C. jejuni strain isolated from a gastroenteritis patient. Mating experiments demonstrated the transference of this genetic element (pCjH01) among C. jejuni by plasmid conjugation. The pCjH01 plasmid was sequenced and assembled, revealing high similarity (97% identity) with pTet, a described tetracycline resistance encoding plasmid. pCjH01 (47.7 kb) is a mosaic plasmid composed of a pTet backbone that has acquired two discrete DNA regions. Remarkably, one of the acquired sequences carried an undescribed variant of the aadE-sat4-aphA-3 gene cluster, providing resistance to at least kanamycin and gentamycin. Aside from the antibiotic resistance genes, the cluster also carries genes coding for putative regulators, such as a sigma factor of the RNA polymerase and an antisigma factor. Homology searches suggest that Campylobacter exchanges genetic material with distant G-positive bacterial genera. Keywords: Campylobacter; conjugative plasmid; antibiotic resistance; aminoglycoside Author: Pedro Guirado, Elisenda Miró, Yaidelis Iglesias-Torrens, Ferran Navarro, Susana Campoy, Tyler Scott Alioto, Jessica Gómez-Garrido, Cristina Madrid and Carlos Balsalobre Creator: LaTeX with hyperref Producer: pdfTeX-1.40.21 CreationDate: Thu Mar 31 07:52:45 2022 CEST ModDate: Thu Mar 31 08:38:43 2022 CEST Tagged: no UserProperties: no Suspects: no Form: none JavaScript: no Pages: 14 Encrypted: no Page size: 595.276 x 841.89 pts (A4) Page rot: 0 File size: 1809188 bytes Optimized: no PDF version: 1.7 name type encoding emb sub uni object ID ------------------------------------ ----------------- ---------------- --- --- --- --------- SOZKOC+VnURWPalladioL-Bold Type 1 Custom yes yes no 10 0 FJMGBM+VnURWPalladioL Type 1 Custom yes yes no 15 0 WTPTCL+URWPalladioL-Bold Type 1 Custom yes yes no 19 0 HCQEGQ+URWPalladioL-Roma Type 1 Custom yes yes no 24 0 REIKRN+URWPalladioL-Ital Type 1 Custom yes yes no 28 0 RSOQSD+URWPalladioL-BoldItal Type 1 Custom yes yes no 32 0 VIWIOF+CMSY10 Type 1 Builtin yes yes no 36 0 MCBCCI+SourceSansRoman-Regular Type 1C Custom yes yes no 44 0 MCBCCM+SourceSansRoman.613wght Type 1C Custom yes yes no 48 0 MCBCCO+SourceSansRoman-Bold Type 1C Custom yes yes no 52 0 YYFPCJ+TimesNewRomanPS-BoldItalicMT Type 1C WinAnsi yes yes no 65 0 LFODOF+PalatinoLinotype,Bold TrueType WinAnsi yes yes no 93 0 LFODOG+PalatinoLinotype CID TrueType Identity-H yes yes yes 96 0 LFODOH+PalatinoLinotype TrueType WinAnsi yes yes no 102 0 LFODOI+PalatinoLinotype,Italic CID TrueType Identity-H yes yes yes 105 0 LFODOJ+PalatinoLinotype,Italic TrueType WinAnsi yes yes no 111 0 LFODOF+PalatinoLinotype,Bold TrueType WinAnsi yes yes no 121 0 LFODOH+PalatinoLinotype TrueType WinAnsi yes yes no 124 0 LFODOJ+PalatinoLinotype,Italic TrueType WinAnsi yes yes no 127 0 LFODOF+PalatinoLinotype,Bold TrueType WinAnsi yes yes no 137 0 LFODOH+PalatinoLinotype TrueType WinAnsi yes yes no 140 0 LFODOJ+PalatinoLinotype,Italic TrueType WinAnsi yes yes no 143 0 LFODOF+PalatinoLinotype,Bold TrueType WinAnsi yes yes no 159 0 LFODOG+PalatinoLinotype CID TrueType Identity-H yes yes yes 162 0 LFODOH+PalatinoLinotype TrueType WinAnsi yes yes no 168 0 LFODOJ+PalatinoLinotype,Italic TrueType WinAnsi yes yes no 171 0 OVNEXW+EURM10 Type 1 Builtin yes yes no 181 0 Jhove (Rel. 1.22.1, 2019-04-17) Date: 2023-02-25 02:36:27 CET RepresentationInformation: antibiotics_11_00466_v2.pdf ReportingModule: PDF-hul, Rel. 1.12.1 (2019-04-17) LastModified: 2023-02-18 01:34:25 CET Size: 1809188 Format: PDF Version: 1.7 Status: Well-Formed and valid SignatureMatches: PDF-hul MIMEtype: application/pdf PDFMetadata: Objects: 373 FreeObjects: 1 IncrementalUpdates: 0 DocumentCatalog: PageLayout: SinglePage PageMode: UseNone Outlines: Item: Title: Introduction Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@44c8afef Item: Title: Results and Discussion Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@7b69c6ba Children: Item: Title: C. jejuni Strain H01 Carries a Conjugative Plasmid Providing Resistance to Tetracycline, Kanamycin, and Gentamicin Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@46daef40 Item: Title: pCjH01 Is Derived from the pTet Plasmid and Carries a New Variant of the aadE-sat4-aphA-3 Gene Cluster Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@12f41634 Item: Title: Conclusions Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@13c27452 Item: Title: Materials and Methods Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@262b2c86 Children: Item: Title: Bacterial Strains, Plasmids, and Growth Conditions Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@371a67ec Item: Title: Mating Experiments Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@5ed828d Item: Title: DNA Techniques Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@50d0686 Item: Title: S1-PFGE and DNA Hybridization Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@7a3d45bd Item: Title: Genome Sequencing, Plasmid Assembly, and Alignment Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@1e7c7811 Item: Title: References Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@77ec78b9 Info: Title: A New Variant of the aadE-sat4-aphA-3 Gene Cluster Found in a Conjugative Plasmid from a MDR Campylobacter jejuni Isolate Author: Pedro Guirado, Elisenda Miró, Yaidelis Iglesias-Torrens, Ferran Navarro, Susana Campoy, Tyler Scott Alioto, Jessica Gómez-Garrido, Cristina Madrid and Carlos Balsalobre Subject: Campylobacter jejuni is a foodborne pathogen causing bacterial gastroenteritis, with the highest incidence reported in Europe. The prevalence of antibiotic resistance in C. jejuni, as well as in many other bacterial pathogens, has increased over the last few years. In this report, we describe the presence of a plasmid in a multi-drug-resistant C. jejuni strain isolated from a gastroenteritis patient. Mating experiments demonstrated the transference of this genetic element (pCjH01) among C. jejuni by plasmid conjugation. The pCjH01 plasmid was sequenced and assembled, revealing high similarity (97% identity) with pTet, a described tetracycline resistance encoding plasmid. pCjH01 (47.7 kb) is a mosaic plasmid composed of a pTet backbone that has acquired two discrete DNA regions. Remarkably, one of the acquired sequences carried an undescribed variant of the aadE-sat4-aphA-3 gene cluster, providing resistance to at least kanamycin and gentamycin. Aside from the antibiotic resistance genes, the cluster also carries genes coding for putative regulators, such as a sigma factor of the RNA polymerase and an antisigma factor. Homology searches suggest that Campylobacter exchanges genetic material with distant G-positive bacterial genera. 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