1a7a7c4b4310c63c7efc1140f8eb7f1d micres_a2022v13n1p102.pdf 78c7462b4bf7381fc56e8619b047986350822a2a micres_a2022v13n1p102.pdf 961e7cd6730c6d62b7554d5ee896b0c2ef6caa986adea159558a624d3b1a733f micres_a2022v13n1p102.pdf Title: First Report of Streptococcus ruminantium in Wildlife: Phenotypic Differences with a Spanish Domestic Ruminant Isolate Subject: Streptococcus ruminantium is a recent reclassification of the former Streptococcus suis serovar 33. Although knowledge about S. suis is extensive, information on S. ruminantium host range and pathogenic potential is still scarce. This bacterium has been isolated from lesions in domestic ruminants, but there are no reports in wild animals. Here, we provide information on lesions associated with S. ruminantium in Pyrenean chamois (Rupicapra pyrenaica) and domestic sheep from NE Spain, as well as phenotypic biopatterns and antimicrobial resistance (AMR) of the isolates. Overall, lesions caused by S. ruminantium were similar to those caused by S. suis, excluding polyserositis. Heterogeneity of the phenotypic profiles was observed within the S. ruminantium strains by VITEK-2, resulting in only two tests common to all S. ruminantium isolates and different from S. suis: Alpha-Galactosidase and Methyl-B-D-Glucopyranoside, both positive for S. suis and negative for S. ruminantium strains. Isolates from Pyrenean chamois were susceptible to all antimicrobials tested, except danofloxacin, whereas the domestic sheep isolate was resistant to tetracycline. In conclusion, S. ruminantium can cause infection and be associated with pathology in both wild and domestic ruminants. Due to its phenotypic diversity, a specific PCR is optimal for identification in routine diagnosis. Keywords: Streptococcus ruminantium; antimicrobial resistance; Pyrenean chamois Author: Carlos Neila-Ibáñez, Ester Pintado, Roser Velarde, Xavier Fernández Aguilar, Enric Vidal, Virginia Aragon and M. Lourdes Abarca Creator: LaTeX with hyperref Producer: pdfTeX-1.40.21 CreationDate: Tue Feb 8 12:01:13 2022 CET ModDate: Tue Feb 8 12:04:26 2022 CET Tagged: no UserProperties: no Suspects: no Form: none JavaScript: no Pages: 12 Encrypted: no Page size: 595.276 x 841.89 pts (A4) Page rot: 0 File size: 1461622 bytes Optimized: no PDF version: 1.7 name type encoding emb sub uni object ID ------------------------------------ ----------------- ---------------- --- --- --- --------- UKXHGC+VnURWPalladioL Type 1 Custom yes yes no 10 0 MJUACC+URWPalladioL-Bold Type 1 Custom yes yes no 15 0 UMLWUQ+URWPalladioL-Roma Type 1 Custom yes yes no 20 0 XCHTIJ+URWPalladioL-Ital Type 1 Custom yes yes no 24 0 DEFFQX+URWPalladioL-BoldItal Type 1 Custom yes yes no 28 0 AIQCCL+VnURWPalladioL-Bold Type 1 Custom yes yes no 32 0 MCBCCI+SourceSansRoman-Regular Type 1C Custom yes yes no 40 0 MCBCCM+SourceSansRoman.613wght Type 1C Custom yes yes no 44 0 MCBCCO+SourceSansRoman-Bold Type 1C Custom yes yes no 48 0 DWWZCG+CMSY10 Type 1 Builtin yes yes no 92 0 OVNEXW+EURM10 Type 1 Builtin yes yes no 96 0 BCDEEE+PalatinoLinotype-Bold TrueType WinAnsi yes yes no 114 0 BCDFEE+PalatinoLinotype-Roman TrueType WinAnsi yes yes no 118 0 BCDGEE+PalatinoLinotype-Italic TrueType WinAnsi yes yes no 122 0 BCDEEE+PalatinoLinotype-Bold TrueType WinAnsi yes yes no 135 0 BCDFEE+PalatinoLinotype-Roman TrueType WinAnsi yes yes no 139 0 BCDGEE+PalatinoLinotype-Italic TrueType WinAnsi yes yes no 143 0 QCCTFG+VnURWPalladioL-Italic Type 1 Custom yes yes no 154 0 Jhove (Rel. 1.22.1, 2019-04-17) Date: 2022-04-06 02:39:39 CEST RepresentationInformation: micres_a2022v13n1p102.pdf ReportingModule: PDF-hul, Rel. 1.12.1 (2019-04-17) LastModified: 2022-03-01 12:02:10 CET Size: 1461622 Format: PDF Version: 1.7 Status: Well-Formed and valid SignatureMatches: PDF-hul MIMEtype: application/pdf PDFMetadata: Objects: 308 FreeObjects: 1 IncrementalUpdates: 0 DocumentCatalog: PageLayout: SinglePage PageMode: UseNone Outlines: Item: Title: Introduction Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@7b69c6ba Item: Title: Materials and Methods Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@46daef40 Children: Item: Title: Bacterial Identification and Genotyping Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@12f41634 Item: Title: Isolates Characterization Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@13c27452 Item: Title: Histopathology Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@262b2c86 Item: Title: Results Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@371a67ec Children: Item: Title: Specific PCR Is a Suitable Method for Identification of S. ruminantium Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@5ed828d Item: Title: S. ruminantium Isolates Presented Phenotypic Differences Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@50d0686 Item: Title: Lesions Associated with S. ruminantium Isolation Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@7a3d45bd Item: Title: Discussion Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@1e7c7811 Item: Title: References Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@77ec78b9 Info: Title: First Report of Streptococcus ruminantium in Wildlife: Phenotypic Differences with a Spanish Domestic Ruminant Isolate Author: Carlos Neila-Ibáñez, Ester Pintado, Roser Velarde, Xavier Fernández Aguilar, Enric Vidal, Virginia Aragon and M. Lourdes Abarca Subject: Streptococcus ruminantium is a recent reclassification of the former Streptococcus suis serovar 33. Although knowledge about S. suis is extensive, information on S. ruminantium host range and pathogenic potential is still scarce. This bacterium has been isolated from lesions in domestic ruminants, but there are no reports in wild animals. Here, we provide information on lesions associated with S. ruminantium in Pyrenean chamois (Rupicapra pyrenaica) and domestic sheep from NE Spain, as well as phenotypic biopatterns and antimicrobial resistance (AMR) of the isolates. Overall, lesions caused by S. ruminantium were similar to those caused by S. suis, excluding polyserositis. Heterogeneity of the phenotypic profiles was observed within the S. ruminantium strains by VITEK-2, resulting in only two tests common to all S. ruminantium isolates and different from S. suis: Alpha-Galactosidase and Methyl-B-D-Glucopyranoside, both positive for S. suis and negative for S. ruminantium strains. Isolates from Pyrenean chamois were susceptible to all antimicrobials tested, except danofloxacin, whereas the domestic sheep isolate was resistant to tetracycline. In conclusion, S. ruminantium can cause infection and be associated with pathology in both wild and domestic ruminants. Due to its phenotypic diversity, a specific PCR is optimal for identification in routine diagnosis. 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