cffccd889bd3e402cab8784519f82f83 animals_a2022v12n5p667.pdf a156e97818be6cd432dfc67b9e26b8ac9cb245af animals_a2022v12n5p667.pdf ac1cde015f46a83aaf73e5df3eddab905ac704e6844649b2e7e9d45c1fc32bee animals_a2022v12n5p667.pdf Title: Changes in Oxidative Status Biomarkers in Saliva and Serum in the Equine Gastric Ulcer Syndrome and Colic of Intestinal Aetiology: A Pilot Study Subject: Changes in the oxidative status of the blood of horses suffering from gastric ulcers and colic of intestinal aetiology (CIE) have been reported. However, saliva can also be a source of biomarkers of oxidative status. Therefore, this study aims to validate automated assays for the measurement of oxidative status biomarkers (ferric reducing ability of saliva/serum—FRAS/FRAP, cupric reducing antioxidant capacity—CUPRAC, the Trolox equivalent antioxidant capacity—TEAC, uric acid, and advanced oxidation protein products—AOPP) in the saliva and serum of horses, to assess their changes in the different ulcer gastric diseases (squamous—ESGD and glandular—EGGD) and CIE, and to evaluate their relationship with serum amyloid A (SAA), adenosine deaminase (ADA), and the systemic inflammatory response syndrome (SIRS) status. The assays showed a low imprecision and good linearity with enough sensitivity in both fluids. In EGGD, higher levels of FRAS, uric acid, and AOPP in saliva were observed compared to the healthy group, correlating with the salivary ADA levels. Horses with CIE showed increases in uric acid concentrations in serum associated with their SIRS status and outcome of the disease. In conclusion, analytes related to the oxidative status can be measured in the saliva and serum from horses by automated assays, and some of them can potentially be assessed as biomarkers in horses with gastric ulcers and CIE. Keywords: horse; EGUS; colic; redox profile; saliva Author: María Dolores Contreras-Aguilar, Camila Peres Rubio, Luis Guillermo González-Arostegui, María Martín-Cuervo, Jose J. Cerón, Ignacio Ayala, Ida-Marie Holm Henriksen, Stine Jacobsen and Sanni Hansen Creator: LaTeX with hyperref Producer: pdfTeX-1.40.21 CreationDate: Mon Mar 7 10:32:37 2022 CET ModDate: Mon Mar 7 13:33:33 2022 CET Tagged: no UserProperties: no Suspects: no Form: none JavaScript: no Pages: 14 Encrypted: no Page size: 595.276 x 841.89 pts (A4) Page rot: 0 File size: 1378274 bytes Optimized: no PDF version: 1.7 name type encoding emb sub uni object ID ------------------------------------ ----------------- ---------------- --- --- --- --------- BFTPJC+VnURWPalladioL Type 1 Custom yes yes no 10 0 URKJRQ+URWPalladioL-Bold Type 1 Custom yes yes no 15 0 GOHQTY+URWPalladioL-Roma Type 1 Custom yes yes no 20 0 HXXXDT+URWPalladioL-Ital Type 1 Custom yes yes no 24 0 PRJKGQ+VnURWPalladioL-Bold Type 1 Custom yes yes no 28 0 MCBCCI+SourceSansRoman-Regular Type 1C Custom yes yes no 36 0 MCBCCM+SourceSansRoman.613wght Type 1C Custom yes yes no 40 0 MCBCCO+SourceSansRoman-Bold Type 1C Custom yes yes no 44 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FNTSBS+TimesNewRomanPS-BoldMT CID TrueType Identity-H yes yes yes 181 0 FNTSBS+TimesNewRomanPS-ItalicMT CID TrueType Identity-H yes yes yes 187 0 FNTSBS+TimesNewRomanPSMT CID TrueType Identity-H yes yes yes 193 0 FNTSBS+MalgunGothic CID TrueType Identity-H yes yes yes 199 0 FNTSBS+SymbolMT CID TrueType Identity-H yes yes yes 205 0 Jhove (Rel. 1.22.1, 2019-04-17) Date: 2022-04-07 02:23:17 CEST RepresentationInformation: animals_a2022v12n5p667.pdf ReportingModule: PDF-hul, Rel. 1.12.1 (2019-04-17) LastModified: 2022-03-21 11:25:15 CET Size: 1378274 Format: PDF Version: 1.7 Status: Well-Formed and valid SignatureMatches: PDF-hul MIMEtype: application/pdf PDFMetadata: Objects: 387 FreeObjects: 1 IncrementalUpdates: 0 DocumentCatalog: PageLayout: SinglePage PageMode: UseNone Outlines: Item: Title: Introduction Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@7b69c6ba Item: Title: Materials and Methods Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@46daef40 Children: Item: Title: Diseased and Healthy Populations Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@12f41634 Item: Title: Sampling Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@13c27452 Item: Title: Salivary and Plasma Biomarkers Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@262b2c86 Item: Title: Analytical Validation of the Oxidative Stress Profile Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@371a67ec Item: Title: Data Analysis Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@5ed828d Item: Title: Results Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@50d0686 Children: Item: Title: Diseased and Healthy Populations Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@7a3d45bd Item: Title: Analytical Validation of the Oxidative Stress Biomarkers Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@1e7c7811 Item: Title: Adenosine Deaminase Results Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@77ec78b9 Item: Title: Changes in the Oxidative Stress Biomarkers in Saliva and Serum between the Healthy and Diseased Populations Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@1a3869f4 Item: Title: Differences in the Salivary and Serum Oxidative Stress Biomarkers depending on the Colic Outcome Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@a38d7a3 Item: Title: Associations between the Salivary and Serum Redox Profiles with the Inflammatory and Immunity Biomarkers Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@77f99a05 Item: Title: Discussion Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@63440df3 Item: Title: Conclusions Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@3aeaafa6 Item: Title: References Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@76a3e297 Info: Title: Changes in Oxidative Status Biomarkers in Saliva and Serum in the Equine Gastric Ulcer Syndrome and Colic of Intestinal Aetiology: A Pilot Study Author: María Dolores Contreras-Aguilar, Camila Peres Rubio, Luis Guillermo González-Arostegui, María Martín-Cuervo, Jose J. Cerón, Ignacio Ayala, Ida-Marie Holm Henriksen, Stine Jacobsen and Sanni Hansen Subject: Changes in the oxidative status of the blood of horses suffering from gastric ulcers and colic of intestinal aetiology (CIE) have been reported. However, saliva can also be a source of biomarkers of oxidative status. Therefore, this study aims to validate automated assays for the measurement of oxidative status biomarkers (ferric reducing ability of saliva/serum FRAS/FRAP, cupric reducing antioxidant capacity CUPRAC, the Trolox equivalent antioxidant capacity TEAC, uric acid, and advanced oxidation protein products AOPP) in the saliva and serum of horses, to assess their changes in the different ulcer gastric diseases (squamous ESGD and glandular EGGD) and CIE, and to evaluate their relationship with serum amyloid A (SAA), adenosine deaminase (ADA), and the systemic inflammatory response syndrome (SIRS) status. The assays showed a low imprecision and good linearity with enough sensitivity in both fluids. In EGGD, higher levels of FRAS, uric acid, and AOPP in saliva were observed compared to the healthy group, correlating with the salivary ADA levels. Horses with CIE showed increases in uric acid concentrations in serum associated with their SIRS status and outcome of the disease. In conclusion, analytes related to the oxidative status can be measured in the saliva and serum from horses by automated assays, and some of them can potentially be assessed as biomarkers in horses with gastric ulcers and CIE. 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