c81ef9678969a7318be9f2b8e6e6ff58 vetsci_a2022v9n3p116.pdf cc16de746f57f3b3da7ce62e3a257d466b640296 vetsci_a2022v9n3p116.pdf 94c972bb4ff45512b8264ae5167a5ee983afa09341cd03e6b7cba76348cf5a5a vetsci_a2022v9n3p116.pdf Title: Humoral Responses and Ex Vivo IFN- Production after Canine Whole Blood Stimulation with Leishmania infantum Antigen or KMP11 Recombinant Protein Subject: The effect of Leishmania infantum soluble antigen (LSA) and recombinant Kinetoplastid Membrane Protein 11 (rKMP11) on the induction of ex vivo specific IFN- (n = 69) and antibody responses (n = 108) was determined in dogs. All dogs were tested for serological response to both antigens and divided into Group 1: healthy (Asturias, Spain, n = 26), Group 2: sick (n = 46), Group 3: healthy Ibizan hounds (Mallorca, Spain, n = 22) and Group 4: healthy (Bari, Italy, n = 14). Antibody levels were higher for LSA when compared to rKMP11 (p = 0.001). Ibizan hounds were all seronegative to rKMP11 and 18% were low seropositive to LSA. Sick dogs presented higher antibody response to both antigens compared to the rest of the groups (p < 0.0001). All groups showed higher IFN- levels after LSA compared to rKMP11 responses (p < 0.05). The highest response to LSA was found in Ibizan hounds (p < 0.05). IFN- to LSA and rKMP11 stimulation was observed in 34% and in 2.8% of the sick dogs, respectively. Here, we demonstrated that anti-rKMP11 antibodies are mainly present in dogs with moderate to severe disease. Furthermore, cellular immune response measured by specific ex vivo IFN- production was more intense to LSA than stimulated to rKMP11. Keywords: leishmaniosis; serology; dog; cell-mediated immunity; diagnosis Author: Pamela Martínez-Orellana, Noemí González, Antonella Baldassarre, Alejandra Álvarez-Fernández, Laura Ordeix, Paola Paradies, Manuel Soto and Laia Solano-Gallego Creator: LaTeX with hyperref Producer: pdfTeX-1.40.21 CreationDate: Fri Mar 4 12:01:09 2022 CET ModDate: Fri Mar 4 12:04:41 2022 CET Tagged: no UserProperties: no Suspects: no Form: none JavaScript: no Pages: 10 Encrypted: no Page size: 595.276 x 841.89 pts (A4) Page rot: 0 File size: 9370218 bytes Optimized: no PDF version: 1.7 name type encoding emb sub uni object ID ------------------------------------ ----------------- ---------------- --- --- --- --------- AKXRSZ+EURB10 Type 1 Builtin yes yes no 10 0 YXDTOY+VnURWPalladioL-Bold Type 1 Custom yes yes no 14 0 GVTJFH+VnURWPalladioL Type 1 Custom yes yes no 19 0 MDJLZU+EURM10 Type 1 Builtin yes yes no 23 0 PLJUNZ+URWPalladioL-Bold Type 1 Custom yes yes no 27 0 YQOYKC+URWPalladioL-Roma Type 1 Custom yes yes no 32 0 XUEXOF+URWPalladioL-Ital Type 1 Custom yes yes no 36 0 HSWRMF+URWPalladioL-BoldItal Type 1 Custom yes yes no 40 0 RUWDWW+EURM7 Type 1 Builtin yes yes no 44 0 MCBCCI+SourceSansRoman-Regular Type 1C Custom yes yes no 52 0 MCBCCM+SourceSansRoman.613wght Type 1C Custom yes yes no 56 0 MCBCCO+SourceSansRoman-Bold Type 1C Custom yes yes no 60 0 CTCXDQ+TimesNewRomanPS-BoldItalicMT Type 1C WinAnsi yes yes no 72 0 PDYDVH+CMSY10 Type 1 Builtin yes yes no 97 0 GEWEQC+PazoMath-Italic Type 1 Builtin yes yes no 104 0 NJWNDJ+VnURWPalladioL-Italic Type 1 Custom yes yes no 143 0 Jhove (Rel. 1.22.1, 2019-04-17) Date: 2022-04-07 02:23:12 CEST RepresentationInformation: vetsci_a2022v9n3p116.pdf ReportingModule: PDF-hul, Rel. 1.12.1 (2019-04-17) LastModified: 2022-03-21 12:45:29 CET Size: 9370218 Format: PDF Version: 1.7 Status: Well-Formed and valid SignatureMatches: PDF-hul MIMEtype: application/pdf PDFMetadata: Objects: 320 FreeObjects: 7 IncrementalUpdates: 0 DocumentCatalog: PageLayout: SinglePage PageMode: UseNone Outlines: Item: Title: Introduction Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@7b69c6ba Item: Title: Materials and Methods Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@46daef40 Children: Item: Title: Dogs Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@12f41634 Item: Title: Antigen Preparation Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@13c27452 Item: Title: Serological Diagnosis by ELISA Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@262b2c86 Children: Item: Title: Leishmania infantum ELISA Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@371a67ec Item: Title: KMP11 ELISA Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@5ed828d Item: Title: Whole Blood Assay Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@50d0686 Item: Title: Sandwich ELISA for the Determination of IFN- Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@7a3d45bd Item: Title: Statistical Analysis Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@1e7c7811 Item: Title: Results Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@77ec78b9 Children: Item: Title: Antibody Response Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@1a3869f4 Item: Title: Ex Vivo IFN Release Whole Blood Assay Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@a38d7a3 Item: Title: Discussion Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@77f99a05 Item: Title: Conclusions Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@63440df3 Item: Title: References Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@3aeaafa6 Info: Title: Humoral Responses and Ex Vivo IFN- Production after Canine Whole Blood Stimulation with Leishmania infantum Antigen or KMP11 Recombinant Protein Author: Pamela Martínez-Orellana, Noemí González, Antonella Baldassarre, Alejandra Álvarez-Fernández, Laura Ordeix, Paola Paradies, Manuel Soto and Laia Solano-Gallego Subject: The effect of Leishmania infantum soluble antigen (LSA) and recombinant Kinetoplastid Membrane Protein 11 (rKMP11) on the induction of ex vivo specific IFN- (n = 69) and antibody responses (n = 108) was determined in dogs. All dogs were tested for serological response to both antigens and divided into Group 1: healthy (Asturias, Spain, n = 26), Group 2: sick (n = 46), Group 3: healthy Ibizan hounds (Mallorca, Spain, n = 22) and Group 4: healthy (Bari, Italy, n = 14). Antibody levels were higher for LSA when compared to rKMP11 (p = 0.001). Ibizan hounds were all seronegative to rKMP11 and 18% were low seropositive to LSA. Sick dogs presented higher antibody response to both antigens compared to the rest of the groups (p < 0.0001). All groups showed higher IFN- levels after LSA compared to rKMP11 responses (p < 0.05). The highest response to LSA was found in Ibizan hounds (p < 0.05). IFN- to LSA and rKMP11 stimulation was observed in 34% and in 2.8% of the sick dogs, respectively. Here, we demonstrated that anti-rKMP11 antibodies are mainly present in dogs with moderate to severe disease. Furthermore, cellular immune response measured by specific ex vivo IFN- production was more intense to LSA than stimulated to rKMP11. 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