7baa43d50b874b21f4d797b52228e5b6 ijmolsci_a2022v23n8p4141.pdf 1a46f218f13c49063d7ec1a6398f3676a8d534e9 ijmolsci_a2022v23n8p4141.pdf b066d15bd01b351965bc5df8d455fa7143d8ebec897d8317d7d8acd106ed45b5 ijmolsci_a2022v23n8p4141.pdf Title: Fatty Acids and Metabolomic Composition of Follicular Fluid Collected from Environments Associated with Good and Poor Oocyte Competence in Goats Subject: In goats, embryo oocyte competence is affected by follicle size regardless the age of the females. In previous studies we have found differences in blastocyst development between oocytes coming of small (<3 mm) and large follicles (>3 mm) in prepubertal (1–2 months-old) goats. Oocyte competence and Follicular Fluid (FF) composition changes throughout follicle growth. The aim of this study was to analyze Fatty Acids (FAs) composition and metabolomic profiles of FF recovered from small and large follicles of prepubertal goats and follicles of adult goats. FAs were analyzed by chromatography and metabolites by 1H-Nuclear Magnetic Resonance (1H-NMR) Spectrometry. The results showed important differences between adult and prepubertal follicles: (a) the presence of ,-glucose in adult and no detection in prepubertal; (b) lactate, -N-(CH3)3 groups and inositol were higher in prepubertal (c) the percentage of Linolenic Acid, Total Saturated Fatty Acids and n-3 PUFAs were higher in adults; and (d) the percentage of Linoleic Acid, total MUFAs, PUFAs, n-6 PUFAs and n-6 PUFAs: n-3 PUFAs ratio were higher in prepubertal goats. Not significant differences were found in follicle size of prepubertal goats, despite the differences in oocyte competence for in vitro embryo production. Keywords: reproductive fluids; fertilization; follicles; omega-3; omega-6; glucose Author: Dolors Izquierdo, Montserrat Roura, Míriam Pérez-Trujillo, Sandra Soto-Heras and María-Teresa Paramio Creator: LaTeX with hyperref Producer: pdfTeX-1.40.21 CreationDate: Tue Apr 19 04:24:44 2022 CEST ModDate: Tue Apr 19 04:36:36 2022 CEST Tagged: no UserProperties: no Suspects: no Form: none JavaScript: no Pages: 15 Encrypted: no Page size: 595.276 x 841.89 pts (A4) Page rot: 0 File size: 1867326 bytes Optimized: no PDF version: 1.7 name type encoding emb sub uni object ID ------------------------------------ ----------------- ---------------- --- --- --- --------- SXQPWE+VnURWPalladioL Type 1 Custom yes yes no 10 0 ACZNUE+EURM10 Type 1 Builtin yes yes no 15 0 LTROYO+URWPalladioL-Bold Type 1 Custom yes yes no 19 0 EUOXXN+URWPalladioL-Roma Type 1 Custom yes yes no 24 0 LEMXEG+URWPalladioL-Ital Type 1 Custom yes yes no 28 0 YSPQJI+VnURWPalladioL-Bold Type 1 Custom yes yes no 32 0 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PJKEOD+PalatinoLinotype TrueType WinAnsi yes yes no 153 0 PJKEOF+PalatinoLinotype,Italic TrueType WinAnsi yes yes no 156 0 PJKEOB+PalatinoLinotype,Bold TrueType WinAnsi yes yes no 167 0 PJKEOC+PalatinoLinotype CID TrueType Identity-H yes yes yes 170 0 PJKEOD+PalatinoLinotype TrueType WinAnsi yes yes no 176 0 PJKEOF+PalatinoLinotype,Italic TrueType WinAnsi yes yes no 179 0 Jhove (Rel. 1.22.1, 2019-04-17) Date: 2022-04-27 02:23:47 CEST RepresentationInformation: ijmolsci_a2022v23n8p4141.pdf ReportingModule: PDF-hul, Rel. 1.12.1 (2019-04-17) LastModified: 2022-04-26 12:51:01 CEST Size: 1867326 Format: PDF Version: 1.7 Status: Well-Formed and valid SignatureMatches: PDF-hul MIMEtype: application/pdf PDFMetadata: Objects: 393 FreeObjects: 1 IncrementalUpdates: 0 DocumentCatalog: PageLayout: SinglePage PageMode: UseNone Outlines: Item: Title: Introduction Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@7b69c6ba Item: Title: Results Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@46daef40 Children: Item: Title: Fatty Acids (FA) Composition of Follicular Fluid (FF) Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@12f41634 Item: Title: Metabolome Composition of FF by 1H-NMR Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@13c27452 Item: Title: Discussion Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@262b2c86 Item: Title: Materials and Methods Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@371a67ec Children: Item: Title: Chemicals and Reagents Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@5ed828d Item: Title: Ovary Collection of Prepubertal Goat and Follicular Fluid Extraction Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@50d0686 Item: Title: Follicular Fluid Extraction of Adult Goats by Laparoscopy Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@7a3d45bd Children: Item: Title: Animal Ethics Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@1e7c7811 Item: Title: Laparoscopy Procedure Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@77ec78b9 Item: Title: Fatty Acids (FA) Analysis by Chromatography Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@1a3869f4 Item: Title: Data Processing and Statistical Analysis for FA Composition Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@a38d7a3 Item: Title: Metabolomic Analysis by 1H-Nuclear Magnetic Resonance (1H-NMR) Spectrometry Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@77f99a05 Children: Item: Title: Samples Preparation for 1H-NMR Spectrometry Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@63440df3 Item: Title: 1H NMR Spectroscopy Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@3aeaafa6 Item: Title: Data Preprocessing and Statistical Analyses of NMR Data Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@76a3e297 Item: Title: Conclusions Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@4d3167f4 Item: Title: References Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@ed9d034 Info: Title: Fatty Acids and Metabolomic Composition of Follicular Fluid Collected from Environments Associated with Good and Poor Oocyte Competence in Goats Author: Dolors Izquierdo, Montserrat Roura, Míriam Pérez-Trujillo, Sandra Soto-Heras and María-Teresa Paramio Subject: In goats, embryo oocyte competence is affected by follicle size regardless the age of the females. In previous studies we have found differences in blastocyst development between oocytes coming of small (<3 mm) and large follicles (>3 mm) in prepubertal (1 2 months-old) goats. Oocyte competence and Follicular Fluid (FF) composition changes throughout follicle growth. The aim of this study was to analyze Fatty Acids (FAs) composition and metabolomic profiles of FF recovered from small and large follicles of prepubertal goats and follicles of adult goats. FAs were analyzed by chromatography and metabolites by 1H-Nuclear Magnetic Resonance (1H-NMR) Spectrometry. The results showed important differences between adult and prepubertal follicles: (a) the presence of ,-glucose in adult and no detection in prepubertal; (b) lactate, -N-(CH3)3 groups and inositol were higher in prepubertal (c) the percentage of Linolenic Acid, Total Saturated Fatty Acids and n-3 PUFAs were higher in adults; and (d) the percentage of Linoleic Acid, total MUFAs, PUFAs, n-6 PUFAs and n-6 PUFAs: n-3 PUFAs ratio were higher in prepubertal goats. Not significant differences were found in follicle size of prepubertal goats, despite the differences in oocyte competence for in vitro embryo production. 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