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Title:          Mild hypothermia and vitrification increase the mRNA expression of cold-inducible proteins in bovine oocytes and cumulus cells
Subject:        Theriogenology, 185 (2022) 16-23. doi:10.1016/j.theriogenology.2022.03.010
Author:         Jaume Gardela
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     Item: 
      Title: Mild hypothermia and vitrification increase the mRNA expression of cold-inducible proteins in bovine oocytes and cumulus cells
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      Children: 
       Item: 
        Title: 1. Introduction
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        Title: 2. Materials and methods
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         Item: 
          Title: 2.1. Bovine COCs recovery and IVM
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         Item: 
          Title: 2.2. Experiment 1: hypothermia mimetic zr17-2 supplementation
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         Item: 
          Title: 2.3. Experiment 2: exogenous CIRBP supplementation
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         Item: 
          Title: 2.4. Oocyte vitrification and warming
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          Title: 2.5. RNA extraction and cDNA synthesis
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          Title: 2.6. Quantitative real-time PCR (qPCR)
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          Title: 2.7. Data analyses
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        Title: 3. Results
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          Title: 3.1. Experiment 1: hypothermia mimetic zr17-2 supplementation
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          Title: 3.2. Experiment 2: exogenous CIRBP supplementation
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          Title: 3.3. Apoptosis gene expression: BAX:BCL2 ratio
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        Title: 4. Discussion
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        Title: Declaration of interest
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        Title: Financial support
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        Title: Acknowledgements
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       Item: 
        Title: References
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    Creator: Elsevier
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