a0c4db9180ba37b5f5e98470eb36289b cells_a2022v11n11p1786.pdf f0a0f0763ff85948400d2b903d323a38a2b20299 cells_a2022v11n11p1786.pdf 12af43cb0ca277ce3854ad880a6c5120b0964459caec8b3582e34e1e5391ed00 cells_a2022v11n11p1786.pdf Title: CD200R1 Contributes to Successful Functional Reinnervation after a Sciatic Nerve Injury Subject: Activating and inhibitory immune receptors play a critical role in regulating systemic and central nervous system (CNS) immune and inflammatory processes. The CD200R1 immunoreceptor induces a restraining signal modulating inflammation and phagocytosis in the CNS under different inflammatory conditions. However, it remains unknown whether CD200R1 has a role in modulating the inflammatory response after a peripheral nerve injury, an essential component of the successful regeneration. Expression of CD200R1 and its ligand CD200 was analyzed during homeostasis and after a sciatic nerve crush injury in C57Bl/6 mice. The role of CD200R1 in Wallerian Degeneration (WD) and nerve regeneration was studied using a specific antibody against CD200R1 injected into the nerve at the time of injury. We found an upregulation of CD200R1 mRNA after injury whereas CD200 was downregulated acutely after nerve injury. Blockade of CD200R1 significantly reduced the acute entrance of both neutrophils and monocytes from blood after nerve injury. When long term regeneration and functional recovery were evaluated, we found that blockade of CD200R1 had a significant effect impairing the spontaneous functional recovery. Taken together, these results show that CD200R1 has a role in mounting a successful acute inflammatory reaction after injury, and contributes to an effective functional recovery. Keywords: CD200R1 blocking antibody; immune receptor; macrophages; regeneration; functional reinnervation; Wallerian Degeneration Author: Bruno Pannunzio, Jesús Amo-Aparicio, Camila Julián, Rubèn López-Vales, Hugo Peluffo and Natalia Lago Creator: LaTeX with hyperref Producer: pdfTeX-1.40.21 CreationDate: Wed Jun 1 06:32:25 2022 CEST ModDate: Wed Jun 1 07:20:46 2022 CEST Tagged: no UserProperties: no Suspects: no Form: none JavaScript: no Pages: 16 Encrypted: no Page size: 595.276 x 841.89 pts (A4) Page rot: 0 File size: 2438558 bytes Optimized: no PDF version: 1.7 name type encoding emb sub uni object ID ------------------------------------ ----------------- ---------------- --- --- --- --------- SXQPWE+VnURWPalladioL Type 1 Custom yes yes no 10 0 TOSBAB+URWPalladioL-Bold Type 1 Custom yes yes no 15 0 DQBRWZ+URWPalladioL-Roma Type 1 Custom yes yes no 20 0 OEWCAH+URWPalladioL-Ital Type 1 Custom yes yes no 24 0 KVEXVN+CMSY10 Type 1 Builtin yes yes no 28 0 DNYTMW+VnURWPalladioL-Bold Type 1 Custom yes yes no 32 0 PFKOHU+TimesNewRomanPS-BoldItalicMT Type 1C WinAnsi yes yes no 50 0 LEVXPH+EURM10 Type 1 Builtin yes yes no 75 0 CIDFont+F3 CID TrueType Identity-H yes no yes 97 0 CIDFont+F1 CID TrueType Identity-H yes no yes 105 0 CIDFont+F2 CID TrueType Identity-H yes no yes 113 0 CIDFont+F4 CID TrueType Identity-H yes no yes 121 0 CIDFont+F3 CID TrueType Identity-H yes no yes 138 0 CIDFont+F1 CID TrueType Identity-H yes no yes 146 0 CIDFont+F2 CID TrueType Identity-H yes no yes 154 0 CIDFont+F3 CID TrueType Identity-H yes no yes 176 0 CIDFont+F1 CID TrueType Identity-H yes no yes 184 0 CIDFont+F2 CID TrueType Identity-H yes no yes 192 0 Jhove (Rel. 1.22.1, 2019-04-17) Date: 2023-06-16 02:30:16 CEST RepresentationInformation: cells_a2022v11n11p1786.pdf ReportingModule: PDF-hul, Rel. 1.12.1 (2019-04-17) LastModified: 2022-06-20 15:07:05 CEST Size: 2438558 Format: PDF Version: 1.7 Status: Well-Formed and valid SignatureMatches: PDF-hul MIMEtype: application/pdf PDFMetadata: Objects: 405 FreeObjects: 1 IncrementalUpdates: 0 DocumentCatalog: PageLayout: SinglePage PageMode: UseNone Outlines: Item: Title: Introduction Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@44c8afef Item: Title: Materials and Methods Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@7b69c6ba Children: Item: Title: Animal Surgery and Treatment Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@46daef40 Item: Title: Histological and Immunohistochemical Procedures Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@12f41634 Item: Title: Flow Cytometry Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@13c27452 Item: Title: Isolation of RNA and QPCR Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@262b2c86 Item: Title: Functional Evaluation Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@371a67ec Item: Title: Bone Marrow-Derived Macrophage (BMDM) Generation and Treatment Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@5ed828d Item: Title: Data Processing and Statistical Analysis Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@50d0686 Item: Title: Results Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@7a3d45bd Children: Item: Title: CD200-CD200R1 Expression in Sciatic Nerve Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@1e7c7811 Item: Title: Blockade of CD200R1 Impairs Functional Recovery Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@77ec78b9 Item: Title: Blockade of CD200R1 Delays Monocyte Recruitment after Sciatic Nerve Injury Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@1a3869f4 Item: Title: Discussion Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@a38d7a3 Item: Title: References Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@77f99a05 Info: Title: CD200R1 Contributes to Successful Functional Reinnervation after a Sciatic Nerve Injury Author: Bruno Pannunzio, Jesús Amo-Aparicio, Camila Julián, Rubèn López-Vales, Hugo Peluffo and Natalia Lago Subject: Activating and inhibitory immune receptors play a critical role in regulating systemic and central nervous system (CNS) immune and inflammatory processes. The CD200R1 immunoreceptor induces a restraining signal modulating inflammation and phagocytosis in the CNS under different inflammatory conditions. However, it remains unknown whether CD200R1 has a role in modulating the inflammatory response after a peripheral nerve injury, an essential component of the successful regeneration. Expression of CD200R1 and its ligand CD200 was analyzed during homeostasis and after a sciatic nerve crush injury in C57Bl/6 mice. The role of CD200R1 in Wallerian Degeneration (WD) and nerve regeneration was studied using a specific antibody against CD200R1 injected into the nerve at the time of injury. We found an upregulation of CD200R1 mRNA after injury whereas CD200 was downregulated acutely after nerve injury. Blockade of CD200R1 significantly reduced the acute entrance of both neutrophils and monocytes from blood after nerve injury. When long term regeneration and functional recovery were evaluated, we found that blockade of CD200R1 had a significant effect impairing the spontaneous functional recovery. Taken together, these results show that CD200R1 has a role in mounting a successful acute inflammatory reaction after injury, and contributes to an effective functional recovery. 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