ffb858d523a1ea6420063ae69c839e09 viruses_a2022v14n6p1235.pdf e7aa6a1425ae0eb6894bf533a047688ca791c643 viruses_a2022v14n6p1235.pdf 98fb307457587942fdf99de517608ec2a1e6f26d1e342c9e8412dabbfbe0b136 viruses_a2022v14n6p1235.pdf Title: Antibody Response Induced by BNT162b2 and mRNA-1273 Vaccines against the SARS-CoV-2 in a Cohort of Healthcare Workers Subject: The aim of this study was to characterize the antibody response induced by SARS-CoV-2 mRNA vaccines in a cohort of healthcare workers. A total of 2247 serum samples were analyzed using the Elecsys® Anti-SARS-CoV-2 S-test (Roche Diagnostics International Ltd., Rotkreuz, Switzerland). Sex, age, body mass index (BMI), arterial hypertension, smoking and time between infection and/or vaccination and serology were considered the confounding factors. Regarding the medians, subjects previously infected with SARS-CoV-2 who preserved their response to the nucleocapsid (N) protein showed higher humoral immunogenicity (BNT162b2: 6456.0 U/mL median; mRNA-1273: 2505.0 U/mL) compared with non-infected (BNT162b2: 867.0 U/mL; mRNA-1273: 2300.5 U/mL) and infected subjects with a lost response to N protein (BNT162b2: 2992.0 U/mL). After controlling for the confounders, a higher response was still observed for mRNA-1273 compared with BNT162b2 in uninfected individuals (FC = 2.35, p < 0.0001) but not in previously infected subjects (1.11 FC, p = 0.1862). The lowest levels of antibodies were detected in previously infected non-vaccinated individuals (39.4 U/mL). Clinical variables previously linked to poor prognoses regarding SARS-CoV-2 infection, such as age, BMI and arterial hypertension, were positively associated with increasing levels of anti-S protein antibody exclusively in infected subjects. The mRNA-1273 vaccine generated a higher antibody response to the S protein than BNT162b2 in non-infected subjects only. Keywords: antibodies; SARS-CoV-2; vaccine; mRNA-1273; BNT162b2 Author: Juan F. Delgado, Antoni Berenguer-Llergo, Germà Julià, Gema Navarro, Mateu Espasa, Sara Rodríguez, Noemí Sánchez, Eva Van Den Eynde, Marta Navarro, Joan Calvet, Jordi Gratacós, Rosa M. Serrano, Pilar Peña and María J. Amengual Creator: LaTeX with hyperref Producer: pdfTeX-1.40.21 CreationDate: Tue Jun 7 09:50:17 2022 CEST ModDate: Tue Jun 7 11:23:17 2022 CEST Tagged: no UserProperties: no Suspects: no Form: none JavaScript: no Pages: 12 Encrypted: no Page size: 595.276 x 841.89 pts (A4) Page rot: 0 File size: 536978 bytes Optimized: no PDF version: 1.7 name type encoding emb sub uni object ID ------------------------------------ ----------------- ---------------- --- --- --- --------- BGPTEF+URWPalladioL-Bold Type 1 Custom yes yes no 10 0 DPXZXH+URWPalladioL-Roma Type 1 Custom yes yes no 15 0 CUPAWI+URWPalladioL-Ital Type 1 Custom yes yes no 19 0 IEFSKB+VnURWPalladioL Type 1 Custom yes yes no 23 0 HSNKGA+VnURWPalladioL-Bold Type 1 Custom yes yes no 28 0 HOWKDX+TimesNewRomanPS-BoldItalicMT Type 1C WinAnsi yes yes no 46 0 FAECIJ+URWPalladioL-BoldItal Type 1 Custom yes yes no 71 0 FXXUVH+CMSY10 Type 1 Builtin yes yes no 75 0 FHFKNH+PalatinoLinotype-Bold CID TrueType Identity-H yes yes yes 95 0 FHFKOI+PalatinoLinotype-Roman CID TrueType Identity-H yes yes yes 101 0 FHFLBI+PalatinoLinotype-Italic CID TrueType Identity-H yes yes yes 107 0 FHFLBJ+PalatinoLinotype-Italic TrueType WinAnsi yes yes no 113 0 FHFLBK+PalatinoLinotype-Bold TrueType WinAnsi yes yes no 116 0 FHFLBL+PalatinoLinotype-Roman TrueType WinAnsi yes yes no 119 0 OVNEXW+EURM10 Type 1 Builtin yes yes no 130 0 Jhove (Rel. 1.22.1, 2019-04-17) Date: 2023-05-30 02:32:14 CEST RepresentationInformation: viruses_a2022v14n6p1235.pdf ReportingModule: PDF-hul, Rel. 1.12.1 (2019-04-17) LastModified: 2022-06-22 11:46:19 CEST Size: 536978 Format: PDF Version: 1.7 Status: Well-Formed and valid SignatureMatches: PDF-hul MIMEtype: application/pdf PDFMetadata: Objects: 279 FreeObjects: 1 IncrementalUpdates: 0 DocumentCatalog: PageLayout: SinglePage PageMode: UseNone Outlines: Item: Title: Introduction Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@44c8afef Item: Title: Materials and Methods Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@7b69c6ba Children: Item: Title: Study Design and Participants Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@46daef40 Item: Title: Analysis of Antibody Response to SARS-CoV-2 S and N Proteins Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@12f41634 Item: Title: Clinical Variables Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@13c27452 Item: Title: Statistical Analysis Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@262b2c86 Item: Title: Results Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@371a67ec Children: Item: Title: Antibody Response to SARS-CoV-2 S Protein Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@5ed828d Item: Title: Association between Clinical Variables and Antibody Response to SARS-CoV-2 S Protein Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@50d0686 Item: Title: Discussion Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@7a3d45bd Item: Title: Conclusions Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@1e7c7811 Item: Title: References Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@77ec78b9 Info: Title: Antibody Response Induced by BNT162b2 and mRNA-1273 Vaccines against the SARS-CoV-2 in a Cohort of Healthcare Workers Author: Juan F. Delgado, Antoni Berenguer-Llergo, Germà Julià, Gema Navarro, Mateu Espasa, Sara Rodríguez, Noemí Sánchez, Eva Van Den Eynde, Marta Navarro, Joan Calvet, Jordi Gratacós, Rosa M. Serrano, Pilar Peña and María J. Amengual Subject: The aim of this study was to characterize the antibody response induced by SARS-CoV-2 mRNA vaccines in a cohort of healthcare workers. A total of 2247 serum samples were analyzed using the Elecsys® Anti-SARS-CoV-2 S-test (Roche Diagnostics International Ltd., Rotkreuz, Switzerland). Sex, age, body mass index (BMI), arterial hypertension, smoking and time between infection and/or vaccination and serology were considered the confounding factors. Regarding the medians, subjects previously infected with SARS-CoV-2 who preserved their response to the nucleocapsid (N) protein showed higher humoral immunogenicity (BNT162b2: 6456.0 U/mL median; mRNA-1273: 2505.0 U/mL) compared with non-infected (BNT162b2: 867.0 U/mL; mRNA-1273: 2300.5 U/mL) and infected subjects with a lost response to N protein (BNT162b2: 2992.0 U/mL). After controlling for the confounders, a higher response was still observed for mRNA-1273 compared with BNT162b2 in uninfected individuals (FC = 2.35, p < 0.0001) but not in previously infected subjects (1.11 FC, p = 0.1862). The lowest levels of antibodies were detected in previously infected non-vaccinated individuals (39.4 U/mL). Clinical variables previously linked to poor prognoses regarding SARS-CoV-2 infection, such as age, BMI and arterial hypertension, were positively associated with increasing levels of anti-S protein antibody exclusively in infected subjects. The mRNA-1273 vaccine generated a higher antibody response to the S protein than BNT162b2 in non-infected subjects only. 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