e2c89648f91fcf437773275eec664cd6 bio_a2022m1v280p121258.pdf ee14404d4f58099bc1b4115ab6106edbcc8fd207 bio_a2022m1v280p121258.pdf 13ffaa6d976d75bd1861aaad946c9400b4299ae19ecc1d5887f1174765ed3a68 bio_a2022m1v280p121258.pdf Title: A multivalent Ara-C-prodrug nanoconjugate achieves selective ablation of leukemic cells in an acute myeloid leukemia mouse model Subject: Biomaterials, 280 (2022) 121258. doi:10.1016/j.biomaterials.2021.121258 Keywords: Targeted protein nanoparticle,Multivalency,Targeted drug delivery,Ara-C prodrug,CXCR4,Acute myeloid leukemia Author: Victor Pallarès Creator: Elsevier Producer: Acrobat Distiller 8.1.0 (Windows) CreationDate: Tue Dec 28 17:19:24 2021 CET ModDate: Tue Dec 28 18:12:43 2021 CET Tagged: yes UserProperties: no Suspects: no Form: none JavaScript: no Pages: 14 Encrypted: no Page size: 595.276 x 793.701 pts Page rot: 0 File size: 15515529 bytes Optimized: yes PDF version: 1.7 name type encoding emb sub uni object ID ------------------------------------ ----------------- ---------------- --- --- --- --------- NQUOLT+CharisSIL CID TrueType Identity-H yes yes yes 837 0 UUKQLN+CharisSIL-Bold CID TrueType Identity-H yes yes yes 842 0 VXCJFZ+Univers Type 1C Builtin yes yes yes 847 0 VMMFCE+TeX_CM_Maths_Symbols Type 1C Builtin yes yes yes 851 0 NNLAUQ+TeX_CM_Roman Type 1C Builtin yes yes yes 855 0 RDTDZP+CharisSIL-Italic CID TrueType Identity-H yes yes yes 859 0 PEFRVX+CharisSIL CID TrueType Identity-H yes yes yes 828 0 DFSCIW+STIX-Regular Type 1C Custom yes yes yes 335 0 DVXHAL+CharisSIL-Italic CID TrueType Identity-H yes yes yes 327 0 NTMBZO+TeX_CM_Maths_Italic Type 1C Builtin yes yes yes 341 0 ROILQV+TeX_CM_Bold_Maths_Symbols Type 1C Builtin yes yes yes 80 0 HOHFHA+TimesNewRomanPSMT CID TrueType Identity-H yes yes yes 345 0 Jhove (Rel. 1.22.1, 2019-04-17) Date: 2022-07-19 02:26:19 CEST RepresentationInformation: bio_a2022m1v280p121258.pdf ReportingModule: PDF-hul, Rel. 1.12.1 (2019-04-17) LastModified: 2022-07-18 20:20:09 CEST Size: 15515529 Format: PDF Version: 1.7 Status: Well-Formed, but not valid SignatureMatches: PDF-hul ID: PDF-HUL-122 ErrorMessage: edu.harvard.hul.ois.jhove.module.pdf.PdfInvalidException: Invalid indirect destination - referenced object 'cor2' cannot be found Offset: 15467955 ID: PDF-HUL-122 ErrorMessage: edu.harvard.hul.ois.jhove.module.pdf.PdfInvalidException: Invalid indirect destination - referenced object 'cor2' cannot be found Offset: 15467955 ID: PDF-HUL-122 ErrorMessage: edu.harvard.hul.ois.jhove.module.pdf.PdfInvalidException: Invalid indirect destination - referenced object 'cor2' cannot be found Offset: 15467955 ID: PDF-HUL-122 ErrorMessage: edu.harvard.hul.ois.jhove.module.pdf.PdfInvalidException: Invalid indirect destination - referenced object 'cor1' cannot be found Offset: 15467955 ID: PDF-HUL-122 ErrorMessage: edu.harvard.hul.ois.jhove.module.pdf.PdfInvalidException: Invalid indirect destination - referenced object 'cor1' cannot be found Offset: 15467955 ID: PDF-HUL-122 ErrorMessage: edu.harvard.hul.ois.jhove.module.pdf.PdfInvalidException: Invalid indirect destination - referenced object 'appsec1' cannot be found Offset: 15467955 ID: PDF-HUL-122 ErrorMessage: edu.harvard.hul.ois.jhove.module.pdf.PdfInvalidException: Invalid indirect destination - referenced object 'appsec1' cannot be found Offset: 15467955 ID: PDF-HUL-122 ErrorMessage: edu.harvard.hul.ois.jhove.module.pdf.PdfInvalidException: Invalid indirect destination - referenced object 'appsec1' cannot be found Offset: 15467955 ID: PDF-HUL-122 ErrorMessage: edu.harvard.hul.ois.jhove.module.pdf.PdfInvalidException: Invalid indirect destination - referenced object 'appsec1' cannot be found Offset: 15467955 MIMEtype: application/pdf Profile: Linearized PDF, Tagged PDF PDFMetadata: Objects: 980 FreeObjects: 1 IncrementalUpdates: 1 DocumentCatalog: PageLayout: SinglePage PageMode: UseOutlines Language: en Outlines: Item: Title: A multivalent Ara-C-prodrug nanoconjugate achieves selective ablation of leukemic cells in an acute myeloid leukemia mouse ... Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@6a1aab78 Children: Item: Title: 1 Introduction Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@462d5aee Item: Title: 2 Material and methods Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@69b0fd6f Children: Item: Title: 2.1 Cell lines Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@757942a1 Item: Title: 2.2 Protein nanoparticles production and purification Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@4a87761d Item: Title: 2.3 T22-GFP-H6-Ara-C conjugation Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@66d1af89 Item: Title: 2.4 Light scattering analysis Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@8646db9 Item: Title: 2.5 Electron microscopy Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@37374a5e Item: Title: 2.6 Fluorescence determination Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@4671e53b Item: Title: 2.7 Confocal laser microscopy Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@2db7a79b Item: Title: 2.8 Internalization and antineoplastic capacities of T22-GFP-H6-Ara-C in CXCR4+ AML cell lines Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@6950e31 Item: Title: 2.9 T22-GFP-H6-Ara-C pharmacokinetics and biodistribution in a subcutaneous (SC) AML mouse model Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@b7dd107 Item: Title: 2.10 Detection of T22-GFP-H6-Ara-C in plasma and urine by western blot Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@42eca56e Item: Title: 2.11 Evaluation of the in vivo antineoplastic effect of T22-GFP-H6-Ara-C in a CXCR4+ AML mouse model Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@52f759d7 Item: Title: 2.12 Hepatic and renal function for toxicity assessment Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@7cbd213e Item: Title: 2.13 Histological and immunohistochemical staining Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@192d3247 Item: Title: 2.14 Statistical analysis Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@3ecd23d9 Item: Title: 3 Results Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@569cfc36 Children: Item: Title: 3.1 Characterization and synthesis of T22-GFP-H6-Ara-C NCs Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@43bd930a Item: Title: 3.2 Internalization capacity of T22-GFP-H6-Ara-C and competition with AMD3100 in CXCR4+ AML cell lines Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@33723e30 Item: Title: 3.3 Antineoplastic effect of T22-GFP-H6-Ara-C in CXCR4+ AML cell lines Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@64f6106c Item: Title: 3.4 Pharmacokinetics and biodistribution of T22-GFP-H6-Ara-C in a CXCR4+ AML mouse model Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@553a3d88 Item: Title: 3.5 T22-GFP-H6-Ara-C dose response study in a CXCR4+ AML mouse model Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@7a30d1e6 Item: Title: 3.6 Comparison of the antineoplastic effect of T22-GFP-H6-Ara-C and free Oligo-Ara-C in a CXCR4+ AML mouse model Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@5891e32e Item: Title: 3.7 Evaluation of toxicity in NC on-target and off-target mouse tissues Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@cb0ed20 Item: Title: 4 Discussion Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@8e24743 Item: Title: 5 Conclusion Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@74a10858 Item: Title: Funding Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@23fe1d71 Item: Title: Credit author statement Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@28ac3dc3 Item: Title: Data availability Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@32eebfca Item: Title: Declaration of competing interest Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@4e718207 Item: Title: Appendix A Supplementary data Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@1d371b2d Item: Title: References Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@543c6f6d Info: Title: A multivalent Ara-C-prodrug nanoconjugate achieves selective ablation of leukemic cells in an acute myeloid leukemia mouse model Author: Victor Pallarès Subject: Biomaterials, 280 (2022) 121258. doi:10.1016/j.biomaterials.2021.121258 Keywords: Targeted protein nanoparticle,Multivalency,Targeted drug delivery,Ara-C prodrug,CXCR4,Acute myeloid leukemia Creator: Elsevier Producer: Acrobat Distiller 8.1.0 (Windows) CreationDate: Tue Dec 28 17:19:24 CET 2021 ModDate: Tue Dec 28 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Italic FontBBox: -716, -533, 1627, 1318 FontFile2: true XMP: application/pdf 10.1016/j.biomaterials.2021.121258 Elsevier Ltd Biomaterials, 280 (2022) 121258. doi:10.1016/j.biomaterials.2021.121258 Targeted protein nanoparticle Multivalency Targeted drug delivery Ara-C prodrug CXCR4 Acute myeloid leukemia A multivalent Ara-C-prodrug nanoconjugate achieves selective ablation of leukemic cells in an acute myeloid leukemia mouse model Victor Pallarès Ugutz Unzueta Aïda Falgàs Anna Aviñó Yáiza Núñez Annabel García-León Laura Sánchez-García Naroa Serna Alberto Gallardo Lorena Alba-Castellón Patricia Álamo Jorge Sierra Lídia Cedó Ramon Eritja Antonio Villaverde Esther Vázquez Isolda Casanova Ramon Mangues journal Biomaterials © 2021 The Authors. 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