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41b4e94887292045871453eb35e3a1027b2110d7aea8323f44fb992db4a60422  natcom_a2022v4v13p1819.pdf

Title:          Structural basis for the SUMO protease activity of the atypical ubiquitin-specific protease USPL1
Subject:        Nature Communications, doi:10.1038/s41467-022-29485-0
Keywords:       
Author:         Ying Li
Creator:        Springer
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     Item: 
      Title: Structural basis for the SUMO protease activity of the atypical ubiquitin-specific protease USPL1
      Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@4eb7f003
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       Item: 
        Title: Results
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         Item: 
          Title: Covalent crosslinked complex between USPL1 and SUMO2 precursor
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         Item: 
          Title: Overall structure of the USPL1-SUMO2 complex
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         Item: 
          Title: Structural comparison with ubiquitin-specific USP members
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         Item: 
          Title: SUMO2 interface of the C-nobreakterminal tail with USPL1
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         Item: 
          Title: SUMO2 Interface with the thumb and fingers subdomains
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         Item: 
          Title: Mutagenesis analysis of the specific interface contacts in USPL1
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       Item: 
        Title: Discussion
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        Title: Methods
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        Children: 
         Item: 
          Title: Plasmids, cloning and point mutation
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         Item: 
          Title: Protein expression and purification
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         Item: 
          Title: Preparation of the USPL1CD- ∆14-SUMO2 DHA complex
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         Item: 
          Title: Crystallization and data collection
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         Item: 
          Title: SUMO-AMC hydrolysis assays
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         Item: 
          Title: In vitro de-SUMOyation assays
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         Item: 
          Title: Intrinsic fluorescence measurements
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       Item: 
        Title: Reporting summary
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       Item: 
        Title: Data availability
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        Title: References
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        Title: Acknowledgements
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        Title: Author contributions
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        Title: Competing interests
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       Item: 
        Title: Additional information
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    Title: Structural basis for the SUMO protease activity of the atypical ubiquitin-specific protease USPL1
    Author: Ying Li
    Subject: Nature Communications, doi:10.1038/s41467-022-29485-0
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