5aa305cf76602c7b24aebe00a16a7536 ANT_a2022v11p1380.pdf e61375b80a4f986cb3e407d439f7b96435256df1 ANT_a2022v11p1380.pdf 2687152344d06b17e53c42fadc9359778634215de9b644330311b7313d176406 ANT_a2022v11p1380.pdf Title: Changes in Biomarkers of Redox Status in Saliva of Pigs after an Experimental Sepsis Induction Subject: Saliva from pigs is gaining attention as an easy sample to obtain, being a source of biomarkers that can provide information on animal health and welfare. This study aimed to evaluate the changes that can occur in salivary biomarkers of the redox status of pigs with an experimentally induced sepsis. For that, the cupric reducing antioxidant capacity (CUPRAC), ferric reducing ability of saliva (FRAS), Trolox equivalent antioxidant capacity (TEAC), advanced oxidation protein products (AOPP), ferrous oxidation-xylenol orange (FOX), peroxide activity (POX-Act), and reactive oxygen-derived compounds (d-ROMs) were measured in the saliva of pigs with experimentally induced sepsis by endotoxin lipopolysaccharide (LPS), non-septic inflammation induced by turpentine, and in healthy individuals before and after 3 h, 6 h, 24 h, and 48 h. AOPP, POX-Act, and d-ROMs in the sepsis group were higher than in the control from 3 h to 24 h after the inoculation. CUPRAC, FRAS, and TEAC were higher in sepsis than the control group at 24 h. These changes were of higher magnitude than those that occurred in the turpentine group. In conclusion, our findings reveal that sepsis produces changes in salivary biomarkers of redox status, which opens the possibility of using them as potential biomarkers in this species. Keywords: antioxidants; cupric; ferric; inflammation; oxidative stress; peroxides Author: María José López-Martínez, Damián Escribano, Alba Ortín-Bustillo, Lorena Franco-Martínez, Luis Guillermo González-Arostegui, José Joaquín Cerón and Camila Peres Rubio Creator: LaTeX with hyperref Producer: pdfTeX-1.40.21 CreationDate: Sat Jul 16 12:08:30 2022 CEST ModDate: Mon Jul 25 08:16:05 2022 CEST Tagged: no UserProperties: no Suspects: no Form: none JavaScript: no Pages: 8 Encrypted: no Page size: 595.276 x 841.89 pts (A4) Page rot: 0 File size: 1062540 bytes Optimized: no PDF version: 1.7 name type encoding emb sub uni object ID ------------------------------------ ----------------- ---------------- --- --- --- --------- MWHIBA+VnURWPalladioL Type 1 Custom yes yes no 10 0 AGAXBH+URWPalladioL-Bold Type 1 Custom yes yes no 15 0 UAYKKC+URWPalladioL-Roma Type 1 Custom yes yes no 20 0 YCXQRV+URWPalladioL-Ital Type 1 Custom yes yes no 24 0 JYPLJJ+VnURWPalladioL-Bold Type 1 Custom yes yes no 28 0 KUDXZB+TimesNewRomanPS-BoldItalicMT Type 1C WinAnsi yes yes no 46 0 QJAFSH+CMSY10 Type 1 Builtin yes yes no 68 0 ZXDKIP+EURM10 Type 1 Builtin yes yes no 75 0 ILXAXG+MSAM10 Type 1 Builtin yes yes no 83 0 BCDEEE+PalatinoLinotype-Bold TrueType WinAnsi yes yes no 91 0 BCDFEE+PalatinoLinotype-Roman TrueType WinAnsi yes yes no 95 0 BCDGEE+PalatinoLinotype-Italic TrueType WinAnsi yes yes no 99 0 BCDHEE+PalatinoLinotype-Roman CID TrueType Identity-H yes yes yes 103 0 TimesNewRomanPSMT CID TrueType Identity-H yes no yes 110 0 BCDEEE+PalatinoLinotype-Bold TrueType WinAnsi yes yes no 134 0 BCDFEE+PalatinoLinotype-Roman TrueType WinAnsi yes yes no 138 0 BCDGEE+PalatinoLinotype-Italic TrueType WinAnsi yes yes no 142 0 BCDHEE+PalatinoLinotype-Roman CID TrueType Identity-H yes yes yes 146 0 TimesNewRomanPSMT CID TrueType Identity-H yes no yes 153 0 Jhove (Rel. 1.22.1, 2019-04-17) Date: 2022-11-10 02:27:39 CET RepresentationInformation: ANT_a2022v11p1380.pdf ReportingModule: PDF-hul, Rel. 1.12.1 (2019-04-17) LastModified: 2022-11-09 11:27:08 CET Size: 1062540 Format: PDF Version: 1.7 Status: Well-Formed and valid SignatureMatches: PDF-hul MIMEtype: application/pdf PDFMetadata: Objects: 309 FreeObjects: 1 IncrementalUpdates: 0 DocumentCatalog: PageLayout: SinglePage PageMode: UseNone Outlines: Item: Title: Introduction Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@4923ab24 Item: Title: Materials and Methods Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@44c8afef Children: Item: Title: Animals, Housing, and Experimental Design Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@7b69c6ba Item: Title: Sampling Procedure Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@46daef40 Item: Title: Assessment of Salivary Biomarkers of Oxidative Status Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@12f41634 Item: Title: Statistical Analysis Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@13c27452 Item: Title: Results Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@262b2c86 Children: Item: Title: Antioxidant Biomarkers Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@371a67ec Item: Title: Oxidant Biomarkers Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@5ed828d Item: Title: Discussion Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@50d0686 Item: Title: Conclusions Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@7a3d45bd Item: Title: References Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@1e7c7811 Info: Title: Changes in Biomarkers of Redox Status in Saliva of Pigs after an Experimental Sepsis Induction Author: María José López-Martínez, Damián Escribano, Alba Ortín-Bustillo, Lorena Franco-Martínez, Luis Guillermo González-Arostegui, José Joaquín Cerón and Camila Peres Rubio Subject: Saliva from pigs is gaining attention as an easy sample to obtain, being a source of biomarkers that can provide information on animal health and welfare. This study aimed to evaluate the changes that can occur in salivary biomarkers of the redox status of pigs with an experimentally induced sepsis. For that, the cupric reducing antioxidant capacity (CUPRAC), ferric reducing ability of saliva (FRAS), Trolox equivalent antioxidant capacity (TEAC), advanced oxidation protein products (AOPP), ferrous oxidation-xylenol orange (FOX), peroxide activity (POX-Act), and reactive oxygen-derived compounds (d-ROMs) were measured in the saliva of pigs with experimentally induced sepsis by endotoxin lipopolysaccharide (LPS), non-septic inflammation induced by turpentine, and in healthy individuals before and after 3 h, 6 h, 24 h, and 48 h. AOPP, POX-Act, and d-ROMs in the sepsis group were higher than in the control from 3 h to 24 h after the inoculation. CUPRAC, FRAS, and TEAC were higher in sepsis than the control group at 24 h. These changes were of higher magnitude than those that occurred in the turpentine group. In conclusion, our findings reveal that sepsis produces changes in salivary biomarkers of redox status, which opens the possibility of using them as potential biomarkers in this species. Keywords: antioxidants; cupric; ferric; inflammation; oxidative stress; peroxides Creator: LaTeX with hyperref Producer: pdfTeX-1.40.21 CreationDate: Sat Jul 16 12:08:30 CEST 2022 ModDate: Mon Jul 25 08:16:05 CEST 2022 ID: 0xbd2620adda4deeb2dfd135e9d927d1c6, 0xbd2620adda4deeb2dfd135e9d927d1c6 Filters: FilterPipeline: FlateDecode Fonts: Type1: Font: BaseFont: ILXAXG+MSAM10 FontSubset: true FirstChar: 4 LastChar: 78 FontDescriptor: FontName: ILXAXG+MSAM10 Flags: Symbolic FontBBox: 8, -463, 1331, 1003 FontFile: true Font: BaseFont: UAYKKC+URWPalladioL-Roma FontSubset: true FirstChar: 2 LastChar: 252 FontDescriptor: FontName: UAYKKC+URWPalladioL-Roma Flags: Symbolic FontBBox: -166, -283, 1021, 943 FontFile: true EncodingDictionary: Differences: true Font: BaseFont: QJAFSH+CMSY10 FontSubset: true FirstChar: 0 LastChar: 48 FontDescriptor: FontName: QJAFSH+CMSY10 Flags: Symbolic FontBBox: -29, -960, 1116, 775 FontFile: true Font: BaseFont: YCXQRV+URWPalladioL-Ital FontSubset: true FirstChar: 44 LastChar: 121 FontDescriptor: FontName: YCXQRV+URWPalladioL-Ital Flags: Symbolic FontBBox: -170, -305, 1010, 941 FontFile: true EncodingDictionary: Differences: true Font: BaseFont: MWHIBA+VnURWPalladioL FontSubset: true FirstChar: 161 LastChar: 226 FontDescriptor: FontName: MWHIBA+VnURWPalladioL Flags: Symbolic FontBBox: -166, -283, 1021, 943 FontFile: true EncodingDictionary: Differences: true Font: BaseFont: ZXDKIP+EURM10 FontSubset: true FirstChar: 11 LastChar: 22 FontDescriptor: FontName: ZXDKIP+EURM10 Flags: Symbolic FontBBox: -32, -243, 1060, 720 FontFile: true Font: BaseFont: JYPLJJ+VnURWPalladioL-Bold FontSubset: true FirstChar: 161 LastChar: 226 FontDescriptor: FontName: JYPLJJ+VnURWPalladioL-Bold Flags: Symbolic FontBBox: -152, -301, 1000, 935 FontFile: true EncodingDictionary: Differences: true Font: BaseFont: AGAXBH+URWPalladioL-Bold FontSubset: true FirstChar: 3 LastChar: 122 FontDescriptor: FontName: AGAXBH+URWPalladioL-Bold Flags: Symbolic FontBBox: -152, -301, 1000, 935 FontFile: true EncodingDictionary: Differences: true Pages: Page: Sequence: 1 Annotations: Annotation: Subtype: Link Rect: 121, 323, 152, 332 Annotation: Subtype: Link Rect: 34, 311, 137, 320 Annotation: Subtype: Link Rect: 34, 164, 96, 186 Annotation: Subtype: Link Rect: 126, 90, 154, 99 Annotation: Subtype: Link Rect: 34, 78, 147, 87 Annotation: Subtype: Link Rect: 34, 756, 175, 792 Annotation: Subtype: Link Rect: 515, 756, 560, 792 Annotation: Subtype: Link Rect: 267, 660, 278, 674 Annotation: Subtype: Link Rect: 497, 660, 508, 674 Annotation: Subtype: Link Rect: 430, 648, 441, 661 Annotation: Subtype: Link Rect: 460, 209, 467, 218 ActionDest: 172 Annotation: Subtype: Link Rect: 467, 209, 474, 218 ActionDest: 172 Annotation: Subtype: Link Rect: 221, 184, 228, 193 ActionDest: 172 Annotation: Subtype: Link Rect: 228, 184, 235, 193 ActionDest: 172 Annotation: Subtype: Link Rect: 189, 108, 196, 117 ActionDest: 172 Annotation: Subtype: Link Rect: 197, 108, 204, 117 ActionDest: 172 Annotation: Subtype: Link Rect: 202, 83, 209, 92 ActionDest: 172 Annotation: Subtype: Link Rect: 270, 71, 277, 80 ActionDest: 172 Annotation: Subtype: Link Rect: 278, 71, 285, 80 ActionDest: 172 Annotation: Subtype: Link Rect: 131, 17, 277, 28 Annotation: Subtype: Link Rect: 392, 17, 560, 28 Annotation: Subtype: Link Rect: 34, 420, 87, 439 Page: Sequence: 2 Annotations: Annotation: Subtype: Link Rect: 531, 723, 543, 732 ActionDest: 172 Annotation: Subtype: Link Rect: 544, 723, 556, 732 ActionDest: 172 Annotation: Subtype: Link Rect: 409, 685, 421, 694 ActionDest: 175 Annotation: Subtype: Link Rect: 231, 647, 243, 657 ActionDest: 175 Annotation: Subtype: Link Rect: 403, 648, 415, 657 ActionDest: 175 Annotation: Subtype: Link Rect: 458, 647, 470, 657 ActionDest: 175 Annotation: Subtype: Link Rect: 508, 647, 520, 657 ActionDest: 175 Annotation: Subtype: Link Rect: 221, 635, 233, 644 ActionDest: 175 Annotation: Subtype: Link Rect: 284, 610, 296, 619 ActionDest: 175 Annotation: Subtype: Link Rect: 208, 207, 220, 216 ActionDest: 175 Annotation: Subtype: Link Rect: 221, 207, 233, 216 ActionDest: 175 Annotation: Subtype: Link Rect: 455, 169, 467, 178 ActionDest: 175 Page: Sequence: 3 Annotations: Annotation: Subtype: Link Rect: 201, 695, 213, 704 ActionDest: 175 Annotation: Subtype: Link Rect: 411, 670, 423, 679 ActionDest: 175 Annotation: Subtype: Link Rect: 533, 644, 545, 654 ActionDest: 175 Annotation: Subtype: Link Rect: 489, 582, 501, 591 ActionDest: 175 Annotation: Subtype: Link Rect: 362, 314, 369, 326 ActionDest: 79 Annotation: Subtype: Link Rect: 242, 301, 249, 313 ActionDest: 79 Annotation: Subtype: Link Rect: 244, 276, 251, 288 ActionDest: 79 Annotation: Subtype: Link Rect: 372, 276, 379, 288 ActionDest: 79 Annotation: Subtype: Link Rect: 523, 276, 530, 288 ActionDest: 79 Annotation: Subtype: Link Rect: 315, 239, 322, 251 ActionDest: 79 Annotation: Subtype: Link Rect: 319, 214, 326, 226 ActionDest: 79 Page: Sequence: 4 Annotations: Annotation: Subtype: Link Rect: 483, 247, 490, 259 ActionDest: 126 Annotation: Subtype: Link Rect: 200, 235, 207, 247 ActionDest: 126 Annotation: Subtype: Link Rect: 351, 197, 358, 209 ActionDest: 126 Annotation: Subtype: Link Rect: 323, 172, 330, 184 ActionDest: 126 Page: Sequence: 5 Annotations: Annotation: Subtype: Link Rect: 339, 168, 351, 177 ActionDest: 175 Annotation: Subtype: Link Rect: 351, 168, 363, 177 ActionDest: 175 Annotation: Subtype: Link Rect: 202, 80, 214, 89 ActionDest: 175 Annotation: Subtype: Link Rect: 214, 80, 226, 89 ActionDest: 175 Page: Sequence: 6 Annotations: Annotation: Subtype: Link Rect: 262, 735, 274, 744 ActionDest: 175 Annotation: Subtype: Link Rect: 243, 723, 255, 732 ActionDest: 175 Annotation: Subtype: Link Rect: 255, 723, 267, 732 ActionDest: 175 Annotation: Subtype: Link Rect: 352, 647, 364, 657 ActionDest: 175 Annotation: Subtype: Link Rect: 219, 547, 231, 556 ActionDest: 175 Annotation: Subtype: Link Rect: 286, 535, 298, 544 ActionDest: 175 Annotation: Subtype: Link Rect: 369, 509, 381, 519 ActionDest: 175 Annotation: Subtype: Link Rect: 382, 509, 394, 519 ActionDest: 175 Annotation: Subtype: Link Rect: 252, 447, 264, 456 ActionDest: 175 Annotation: Subtype: Link Rect: 196, 258, 208, 267 ActionDest: 175 Annotation: Subtype: Link Rect: 399, 233, 411, 242 ActionDest: 175 Page: Sequence: 7 Annotations: Annotation: Subtype: Link Rect: 525, 733, 560, 744 Annotation: Subtype: Link Rect: 165, 721, 373, 732 Annotation: Subtype: Link Rect: 305, 675, 316, 684 ActionDest: 175 Annotation: Subtype: Link Rect: 359, 352, 396, 363 Annotation: Subtype: Link Rect: 402, 352, 439, 363 Annotation: Subtype: Link Rect: 519, 329, 557, 340 Annotation: Subtype: Link Rect: 59, 319, 95, 329 Annotation: Subtype: Link Rect: 363, 306, 400, 317 Annotation: Subtype: Link Rect: 59, 284, 96, 294 Annotation: Subtype: Link Rect: 102, 284, 138, 294 Annotation: Subtype: Link Rect: 290, 250, 327, 260 Annotation: Subtype: Link Rect: 458, 214, 496, 225 Annotation: Subtype: Link Rect: 386, 191, 423, 202 Annotation: Subtype: Link Rect: 59, 169, 96, 179 Annotation: Subtype: Link Rect: 59, 135, 96, 145 Annotation: Subtype: Link Rect: 451, 111, 488, 122 Annotation: Subtype: Link Rect: 152, 77, 190, 87 Annotation: Subtype: Link Rect: 196, 77, 232, 87 Page: Sequence: 8 Annotations: Annotation: Subtype: Link Rect: 95, 722, 133, 733 Annotation: Subtype: Link Rect: 139, 722, 175, 733 Annotation: Subtype: Link Rect: 59, 688, 96, 698 Annotation: Subtype: Link Rect: 102, 688, 138, 698 Annotation: Subtype: Link Rect: 341, 664, 378, 675 Annotation: Subtype: Link Rect: 385, 664, 421, 675 Annotation: Subtype: Link Rect: 403, 641, 441, 652 Annotation: Subtype: Link Rect: 447, 641, 483, 652 Annotation: Subtype: Link Rect: 205, 607, 242, 617 Annotation: Subtype: Link Rect: 59, 573, 96, 583 Annotation: Subtype: Link Rect: 411, 549, 449, 560 Annotation: Subtype: Link Rect: 255, 526, 292, 537 Annotation: Subtype: Link Rect: 361, 503, 399, 514 Annotation: Subtype: Link Rect: 415, 480, 453, 491 Annotation: Subtype: Link Rect: 459, 480, 495, 491 Annotation: Subtype: Link Rect: 113, 458, 151, 468 Annotation: Subtype: Link Rect: 157, 458, 193, 468 Annotation: Subtype: Link Rect: 488, 434, 526, 445 Annotation: Subtype: Link Rect: 283, 376, 320, 387 Annotation: Subtype: Link Rect: 471, 365, 508, 376 Annotation: Subtype: Link Rect: 122, 343, 160, 353 Annotation: Subtype: Link Rect: 440, 319, 477, 330 Annotation: Subtype: Link Rect: 383, 296, 420, 307 Annotation: Subtype: Link Rect: 388, 273, 426, 284 Annotation: Subtype: Link Rect: 381, 250, 419, 261 Annotation: Subtype: Link Rect: 425, 250, 461, 261 Annotation: Subtype: Link Rect: 375, 227, 412, 238 Annotation: Subtype: Link Rect: 419, 227, 455, 238 Annotation: Subtype: Link Rect: 235, 205, 273, 215 Annotation: Subtype: Link Rect: 463, 181, 501, 192 Annotation: Subtype: Link Rect: 507, 181, 543, 192 Annotation: Subtype: Link Rect: 118, 147, 155, 157 Annotation: Subtype: Link Rect: 309, 113, 346, 123 Checksum: f6407eb2 Type: CRC32 Checksum: 5aa305cf76602c7b24aebe00a16a7536 Type: MD5 Checksum: e61375b80a4f986cb3e407d439f7b96435256df1 Type: SHA-1