b827940a31fc70a386cc1cddb38852b8 265981.pdf fba2771a21869445dedd455cca195eb97a516751 265981.pdf 2785019f22a5569aa51741e5f9185127e76f4345aff2fa1f2b63e87c266dac12 265981.pdf Title: Measuring T-Cell Responses against SARS-CoV-2 Is of Utility for Disease and Vaccination Management Subject: The measurement of specific T-cell responses can be a useful tool for COVID-19 diagnostics and clinical management. In this study, we evaluated the IFN- T-cell response against the main SARS-CoV-2 antigens (spike, nucleocapsid and membrane) in acute and convalescent individuals classified according to severity, and in vaccinated and unvaccinated controls. IgG against spike and nucleocapsid were also measured. Spike antigen triggered the highest number of T-cell responses. Acute patients showed a low percentage of positive responses when compared to convalescent (71.6% vs. 91.7%, respectively), but increased during hospitalization and with severity. Some convalescent patients showed an IFN- T-cell response more than 200 days after diagnosis. Only half of the vaccinated individuals displayed an IFN- T-cell response after the second dose. IgG response was found in a higher percentage of individuals compared to IFN- T-cell responses, and moderate correlations between both responses were seen. However, in some acute COVID-19 patients specific T-cell response was detected, but not IgG production. We found that the chances of an IFN- T-cell response against SARS-CoV-2 is low during acute phase, but may increase over time, and that only half of the vaccinated individuals had an IFN- T-cell response after the second dose. Keywords: SARS-CoV-2; T-cell response; humoral response; IFN-; vaccination; ELISPOT Author: Guillem Safont, Irene Latorre, Raquel Villar-Hernández, Zoran Stojanovic, Alicia Marín, Cristina Pérez-Cano, Alicia Lacoma, Bárbara Molina-Moya, Alan Jhunior Solis, Fernando Arméstar, Joan Matllo, Sergio Díaz-Fernández, Arnau Cendón, Liliya Sokalchuk, Guillermo Tolosa, Irma Casas, Antoni Rosell and José Domínguez Creator: LaTeX with hyperref Producer: pdfTeX-1.40.21 CreationDate: Thu Sep 1 11:39:19 2022 CEST ModDate: Thu Sep 1 11:44:07 2022 CEST Tagged: no UserProperties: no Suspects: no Form: none JavaScript: no Pages: 14 Encrypted: no Page size: 595.276 x 841.89 pts (A4) Page rot: 0 File size: 921532 bytes Optimized: no PDF version: 1.7 name type encoding emb sub uni object ID ------------------------------------ ----------------- ---------------- --- --- --- --------- JYPLJJ+VnURWPalladioL-Bold Type 1 Custom yes yes no 10 0 HEOTNM+VnURWPalladioL Type 1 Custom yes yes no 15 0 LEVXPH+EURM10 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and valid SignatureMatches: PDF-hul MIMEtype: application/pdf PDFMetadata: Objects: 502 FreeObjects: 1 IncrementalUpdates: 0 DocumentCatalog: PageLayout: SinglePage PageMode: UseNone Outlines: Item: Title: Introduction Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@44c8afef Item: Title: Materials and Methods Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@7b69c6ba Children: Item: Title: Study Groups and Clinical Definitions Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@46daef40 Item: Title: Peripheral Blood Mononuclear Cells (PBMCs) Isolation and Cryopreservation Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@12f41634 Item: Title: ELISPOT Assay for IFN- T-Cell Response Detection Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@13c27452 Item: Title: ELISA for Humoral Response Detection Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@262b2c86 Item: Title: Statistical Analysis Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@371a67ec Item: Title: Results Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@5ed828d Children: Item: Title: Positivity Rate between Patient Groups Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@50d0686 Item: Title: Quantitative IFN- Response against SARS-CoV-2 Antigens Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@7a3d45bd Item: Title: IFN- Response According to Days after Vaccination and after COVID-19 Diagnosis Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@1e7c7811 Item: Title: T-Cell IFN- Production and Humoral Responses Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@77ec78b9 Item: Title: Discussion Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@1a3869f4 Item: Title: References Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@a38d7a3 Info: Title: Measuring T-Cell Responses against SARS-CoV-2 Is of Utility for Disease and Vaccination Management Author: Guillem Safont, Irene Latorre, Raquel Villar-Hernández, Zoran Stojanovic, Alicia Marín, Cristina Pérez-Cano, Alicia Lacoma, Bárbara Molina-Moya, Alan Jhunior Solis, Fernando Arméstar, Joan Matllo, Sergio Díaz-Fernández, Arnau Cendón, Liliya Sokalchuk, Guillermo Tolosa, Irma Casas, Antoni Rosell and José Domínguez Subject: The measurement of specific T-cell responses can be a useful tool for COVID-19 diagnostics and clinical management. In this study, we evaluated the IFN- T-cell response against the main SARS-CoV-2 antigens (spike, nucleocapsid and membrane) in acute and convalescent individuals classified according to severity, and in vaccinated and unvaccinated controls. IgG against spike and nucleocapsid were also measured. Spike antigen triggered the highest number of T-cell responses. Acute patients showed a low percentage of positive responses when compared to convalescent (71.6% vs. 91.7%, respectively), but increased during hospitalization and with severity. Some convalescent patients showed an IFN- T-cell response more than 200 days after diagnosis. Only half of the vaccinated individuals displayed an IFN- T-cell response after the second dose. IgG response was found in a higher percentage of individuals compared to IFN- T-cell responses, and moderate correlations between both responses were seen. However, in some acute COVID-19 patients specific T-cell response was detected, but not IgG production. We found that the chances of an IFN- T-cell response against SARS-CoV-2 is low during acute phase, but may increase over time, and that only half of the vaccinated individuals had an IFN- T-cell response after the second dose. 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