fe12f0158a98214dce9c74e9b52df10f JouFun_a2022v8n10p1066.pdf d2c67d0e26980ee2fe2f341b2cc06ff87f77f905 JouFun_a2022v8n10p1066.pdf aed4f313e4d8c7b913772d4b1d3d631bf838089657c8501ba081c9ea079d1d23 JouFun_a2022v8n10p1066.pdf Title: Fungal Hal3 (and Its Close Relative Cab3) as Moonlighting Proteins Subject: Hal3 (Sis2) is a yeast protein that was initially identified as a regulatory subunit of the Saccharomyces cerevisiae Ser/Thr protein phosphatase Ppz1. A few years later, it was shown to participate in the formation of an atypical heterotrimeric phosphopantothenoylcysteine decarboxylase (PPCDC) enzyme, thus catalyzing a key reaction in the pathway leading to Coenzyme A biosynthesis. Therefore, Hal3 was defined as a moonlighting protein. The structure of Hal3 in some fungi is made of a conserved core, similar to bacterial or mammalian PPCDCs; meanwhile, in others, the gene encodes a larger protein with N- and C-terminal extensions. In this work, we describe how Hal3 (and its close relative Cab3) participates in these disparate functions and we review recent findings that could make it possible to predict which of these two proteins will show moonlighting properties in fungi. Keywords: protein phosphatase; Ppz1; Vhs3; CoA biosynthesis; heterotrimer Author: Antonio Casamayor and Joaquín Ariño Creator: LaTeX with hyperref Producer: pdfTeX-1.40.21 CreationDate: Tue Oct 11 10:45:16 2022 CEST ModDate: Tue Oct 11 10:49:52 2022 CEST Tagged: no UserProperties: no Suspects: no Form: none JavaScript: no Pages: 13 Encrypted: no Page size: 595.276 x 841.89 pts (A4) Page rot: 0 File size: 2068879 bytes Optimized: no PDF version: 1.7 name type encoding emb sub uni object ID ------------------------------------ ----------------- ---------------- --- --- --- --------- WLENKM+VnURWPalladioL-Bold Type 1 Custom yes yes no 10 0 AHRQMW+VnURWPalladioL Type 1 Custom yes yes no 15 0 HJXKTC+URWPalladioL-Bold Type 1 Custom yes yes no 19 0 ASUNYQ+URWPalladioL-Roma Type 1 Custom yes yes no 24 0 YHOIYZ+URWPalladioL-Ital Type 1 Custom yes yes no 28 0 BSDWLC+TimesNewRomanPSMT Type 1C WinAnsi yes yes no 47 0 LUDWNL+TimesNewRomanPS-BoldItalicMT Type 1C WinAnsi yes yes no 50 0 MPUDFL+PazoMath Type 1 Builtin yes yes no 72 0 QDCWHF+EURM10 Type 1 Builtin yes yes no 76 0 AOPHAZ+CMSY10 Type 1 Builtin yes yes no 86 0 FDLOAD+PalatinoLinotype,Bold TrueType WinAnsi yes yes no 93 0 FDLOAE+PalatinoLinotype CID TrueType Identity-H yes yes yes 96 0 FDLOAF+PalatinoLinotype TrueType WinAnsi yes yes no 102 0 FDLOAH+PalatinoLinotype,Italic TrueType WinAnsi yes yes no 105 0 FDLOAD+PalatinoLinotype,Bold TrueType WinAnsi yes yes no 113 0 FDLOAE+PalatinoLinotype CID TrueType Identity-H yes yes yes 116 0 FDLOAF+PalatinoLinotype TrueType WinAnsi yes yes no 122 0 FDLOAH+PalatinoLinotype,Italic TrueType WinAnsi yes yes no 125 0 FDLOAD+PalatinoLinotype,Bold TrueType WinAnsi yes yes no 136 0 FDLOAF+PalatinoLinotype TrueType WinAnsi yes yes no 139 0 FDLOAH+PalatinoLinotype,Italic TrueType WinAnsi yes yes no 142 0 FDLOAD+PalatinoLinotype,Bold TrueType WinAnsi yes yes no 161 0 FDLOAF+PalatinoLinotype TrueType WinAnsi yes yes no 164 0 FDLOAH+PalatinoLinotype,Italic TrueType WinAnsi yes yes no 167 0 Jhove (Rel. 1.22.1, 2019-04-17) Date: 2023-07-12 02:32:22 CEST RepresentationInformation: JouFun_a2022v8n10p1066.pdf ReportingModule: PDF-hul, Rel. 1.12.1 (2019-04-17) LastModified: 2023-07-11 17:36:56 CEST Size: 2068879 Format: PDF Version: 1.7 Status: Well-Formed and valid SignatureMatches: PDF-hul MIMEtype: application/pdf PDFMetadata: Objects: 397 FreeObjects: 1 IncrementalUpdates: 0 DocumentCatalog: PageLayout: SinglePage PageMode: UseNone Outlines: Item: Title: Introduction Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@44c8afef Item: Title: Identification of Hal3 as an Inhibitor of the Ser/Thr Protein Phosphatase Ppz1 Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@7b69c6ba Item: Title: Hal3 (and Vhs3) as Moonlighting Proteins: Their Involvement in CoA Biosynthesis Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@46daef40 Item: Title: Structural Insights into the Moonlighting Functions of Hal3 Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@12f41634 Item: Title: The Moonlighting Capacity of Hal3 (and Cab3) across Fungi Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@13c27452 Item: Title: Concluding Remarks Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@262b2c86 Item: Title: References Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@371a67ec Info: Title: Fungal Hal3 (and Its Close Relative Cab3) as Moonlighting Proteins Author: Antonio Casamayor and Joaquín Ariño Subject: Hal3 (Sis2) is a yeast protein that was initially identified as a regulatory subunit of the Saccharomyces cerevisiae Ser/Thr protein phosphatase Ppz1. A few years later, it was shown to participate in the formation of an atypical heterotrimeric phosphopantothenoylcysteine decarboxylase (PPCDC) enzyme, thus catalyzing a key reaction in the pathway leading to Coenzyme A biosynthesis. Therefore, Hal3 was defined as a moonlighting protein. The structure of Hal3 in some fungi is made of a conserved core, similar to bacterial or mammalian PPCDCs; meanwhile, in others, the gene encodes a larger protein with N- and C-terminal extensions. In this work, we describe how Hal3 (and its close relative Cab3) participates in these disparate functions and we review recent findings that could make it possible to predict which of these two proteins will show moonlighting properties in fungi. 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