836b51f517e9a5626fc32c59c1ec5be6 nutrients-14-04966.pdf f9c0f3d7b5856a65a5d477598aee7fb76cc8f689 nutrients-14-04966.pdf 5ea55fad3727e4adf71dba4b45487bbecaf6a73f87334cd429af2a60dcfc3c15 nutrients-14-04966.pdf Title: Association of the Gut Microbiota with the Host’s Health through an Analysis of Biochemical Markers, Dietary Estimation, and Microbial Composition Subject: This study aims to analyze the relationship between gut microbiota composition and health parameters through specific biochemical markers and food consumption patterns in the Spanish population. This research includes 60 Spanish adults aged 47.3 11.2 years old. Biochemical and anthropometric measurements, and a self-referred dietary survey (food frequency questionnaire), were analyzed and compared with the participant´s gut microbiota composition analyzed by 16s rDNA sequencing. Several bacterial strains differed significantly with the biochemical markers analyzed, suggesting an involvement in the participant´s metabolic health. Lower levels of Lactobacillaceae and Oscillospiraceae and an increase in Pasteurellaceae, Phascolarctobacterium, and Haemophilus were observed in individuals with higher AST levels. Higher levels of the Christensenellaceae and a decrease in Peptococcaceae were associated with higher levels of HDL-c. High levels of Phascolarctobacterium and Peptococcus and low levels of Butyricicoccus were found in individuals with higher insulin levels. This study also identified associations between bacteria and specific food groups, such as an increase in lactic acid bacteria with the consumption of fermented dairy products or an increase in Verrucomicrobiaceae with the consumption of olive oil. In conclusion, this study reinforces the idea that specific food groups can favorably modulate gut microbiota composition and have an impact on host´s health. Keywords: gut microbiota; dysbiosis; metabolic health; insulin resistance; diet Author: Maite Villaseñor-Aranguren, Carles Rosés, José Ignacio Riezu-Boj, Miguel López-Yoldi, Omar Ramos-Lopez, Anna M. Barceló and Fermín I. Milagro Creator: LaTeX with hyperref Producer: pdfTeX-1.40.21 CreationDate: Wed Nov 23 10:04:17 2022 CET ModDate: Wed Nov 23 10:09:17 2022 CET Tagged: no UserProperties: no Suspects: no Form: none JavaScript: no Pages: 13 Encrypted: no Page size: 595.276 x 841.89 pts (A4) Page rot: 0 File size: 2442269 bytes Optimized: no PDF version: 1.7 name type encoding emb sub uni object ID ------------------------------------ ----------------- ---------------- --- --- --- --------- XWOCXD+VnURWPalladioL-Bold Type 1 Custom yes yes no 10 0 DOMHYP+VnURWPalladioL Type 1 Custom yes yes no 15 0 BOLMEN+SFRM0900 Type 1 Custom yes yes no 19 0 RKEFPE+URWPalladioL-Bold Type 1 Custom yes yes no 24 0 EZEWOI+URWPalladioL-Roma Type 1 Custom yes yes no 29 0 VMQBCS+URWPalladioL-Ital Type 1 Custom yes yes no 33 0 IVXOCJ+CMSY10 Type 1 Builtin yes yes no 37 0 HGMNRX+TimesNewRomanPS-BoldItalicMT Type 1C WinAnsi yes yes no 55 0 OVNEXW+EURM10 Type 1 Builtin yes yes no 80 0 URYLRZ+URWPalladioL-BoldItal Type 1 Custom yes yes no 87 0 POGPNO+PalatinoLinotype,Bold TrueType WinAnsi yes yes no 104 0 POGPOA+PalatinoLinotype TrueType WinAnsi yes yes no 107 0 POGPPB+PalatinoLinotype,Italic TrueType WinAnsi yes yes no 110 0 POGPNO+PalatinoLinotype,Bold TrueType WinAnsi yes yes no 136 0 POGPOA+PalatinoLinotype TrueType WinAnsi yes yes no 139 0 POGPPB+PalatinoLinotype,Italic TrueType WinAnsi yes yes no 142 0 POGPNO+PalatinoLinotype,Bold TrueType WinAnsi yes yes no 157 0 POGPOA+PalatinoLinotype TrueType WinAnsi yes yes no 160 0 POGPPB+PalatinoLinotype,Italic TrueType WinAnsi yes yes no 163 0 POGPNO+PalatinoLinotype,Bold TrueType WinAnsi yes yes no 177 0 POGPOA+PalatinoLinotype TrueType WinAnsi yes yes no 180 0 POGPPB+PalatinoLinotype,Italic TrueType WinAnsi yes yes no 183 0 Jhove (Rel. 1.22.1, 2019-04-17) Date: 2023-06-24 02:38:28 CEST RepresentationInformation: nutrients-14-04966.pdf ReportingModule: PDF-hul, Rel. 1.12.1 (2019-04-17) LastModified: 2023-06-23 14:36:00 CEST Size: 2442269 Format: PDF Version: 1.7 Status: Well-Formed and valid SignatureMatches: PDF-hul MIMEtype: application/pdf PDFMetadata: Objects: 339 FreeObjects: 1 IncrementalUpdates: 0 DocumentCatalog: PageLayout: SinglePage PageMode: UseNone Outlines: Item: Title: Introduction Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@44c8afef Item: Title: Materials and Methods Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@7b69c6ba Children: Item: Title: Participants Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@46daef40 Item: Title: Anthropometric and Biochemical Measurements Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@12f41634 Item: Title: Dietary Estimation Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@13c27452 Item: Title: Fecal Sample Collection, DNA Extraction, and Metagenomic Data Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@262b2c86 Item: Title: Statistical Analysis Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@371a67ec Item: Title: Results Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@5ed828d Children: Item: Title: Characteristics of the Study Population Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@50d0686 Item: Title: Dietary Intake Characteristics Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@7a3d45bd Item: Title: Microbiota Composition: Biochemical Markers Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@1e7c7811 Item: Title: Microbiota Composition: Nutritional Markers Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@77ec78b9 Item: Title: Discussion Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@1a3869f4 Children: Item: Title: Analysis of Microbiota with Metabolic and Hepatic Health Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@a38d7a3 Item: Title: Analysis of Microbiota and Dietary Intake Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@77f99a05 Item: Title: Conclusions Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@63440df3 Item: Title: References Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@3aeaafa6 Info: Title: Association of the Gut Microbiota with the Host’s Health through an Analysis of Biochemical Markers, Dietary Estimation, and Microbial Composition Author: Maite Villaseñor-Aranguren, Carles Rosés, José Ignacio Riezu-Boj, Miguel López-Yoldi, Omar Ramos-Lopez, Anna M. Barceló and Fermín I. Milagro Subject: This study aims to analyze the relationship between gut microbiota composition and health parameters through specific biochemical markers and food consumption patterns in the Spanish population. This research includes 60 Spanish adults aged 47.3 11.2 years old. Biochemical and anthropometric measurements, and a self-referred dietary survey (food frequency questionnaire), were analyzed and compared with the participant´s gut microbiota composition analyzed by 16s rDNA sequencing. Several bacterial strains differed significantly with the biochemical markers analyzed, suggesting an involvement in the participant´s metabolic health. Lower levels of Lactobacillaceae and Oscillospiraceae and an increase in Pasteurellaceae, Phascolarctobacterium, and Haemophilus were observed in individuals with higher AST levels. Higher levels of the Christensenellaceae and a decrease in Peptococcaceae were associated with higher levels of HDL-c. High levels of Phascolarctobacterium and Peptococcus and low levels of Butyricicoccus were found in individuals with higher insulin levels. This study also identified associations between bacteria and specific food groups, such as an increase in lactic acid bacteria with the consumption of fermented dairy products or an increase in Verrucomicrobiaceae with the consumption of olive oil. In conclusion, this study reinforces the idea that specific food groups can favorably modulate gut microbiota composition and have an impact on host´s health. Keywords: gut microbiota; dysbiosis; metabolic health; insulin resistance; diet Creator: LaTeX with hyperref Producer: pdfTeX-1.40.21 CreationDate: Wed Nov 23 10:04:17 CET 2022 ModDate: Wed Nov 23 10:09:17 CET 2022 ID: 0x055ba5b85f6b216b3acce2ef9ed480e4, 0x055ba5b85f6b216b3acce2ef9ed480e4 Filters: FilterPipeline: FlateDecode Fonts: Type1: Font: BaseFont: OVNEXW+EURM10 FontSubset: true FirstChar: 22 LastChar: 22 FontDescriptor: FontName: OVNEXW+EURM10 Flags: Symbolic FontBBox: -32, -243, 1060, 720 FontFile: true Font: BaseFont: VMQBCS+URWPalladioL-Ital FontSubset: true FirstChar: 40 LastChar: 121 FontDescriptor: FontName: VMQBCS+URWPalladioL-Ital Flags: Symbolic FontBBox: -170, -305, 1010, 941 FontFile: true EncodingDictionary: Differences: true Font: BaseFont: BOLMEN+SFRM0900 FontSubset: true FirstChar: 180 LastChar: 180 FontDescriptor: FontName: BOLMEN+SFRM0900 Flags: Symbolic FontBBox: -196, -322, 1502, 937 FontFile: true EncodingDictionary: Differences: true Font: BaseFont: IVXOCJ+CMSY10 FontSubset: true FirstChar: 0 LastChar: 48 FontDescriptor: FontName: IVXOCJ+CMSY10 Flags: Symbolic FontBBox: -29, -960, 1116, 775 FontFile: true Font: BaseFont: URYLRZ+URWPalladioL-BoldItal FontSubset: true FirstChar: 110 LastChar: 110 FontDescriptor: FontName: URYLRZ+URWPalladioL-BoldItal Flags: Symbolic FontBBox: -170, -300, 1073, 935 FontFile: true EncodingDictionary: Differences: true Font: BaseFont: RKEFPE+URWPalladioL-Bold FontSubset: true FirstChar: 3 LastChar: 241 FontDescriptor: FontName: RKEFPE+URWPalladioL-Bold Flags: Symbolic FontBBox: -152, -301, 1000, 935 FontFile: true EncodingDictionary: Differences: true Font: BaseFont: XWOCXD+VnURWPalladioL-Bold FontSubset: true FirstChar: 178 LastChar: 226 FontDescriptor: FontName: XWOCXD+VnURWPalladioL-Bold Flags: Symbolic FontBBox: -152, -301, 1000, 935 FontFile: true EncodingDictionary: Differences: true Font: BaseFont: EZEWOI+URWPalladioL-Roma FontSubset: true FirstChar: 2 LastChar: 243 FontDescriptor: FontName: EZEWOI+URWPalladioL-Roma Flags: Symbolic FontBBox: -166, -283, 1021, 943 FontFile: true EncodingDictionary: Differences: true Font: BaseFont: DOMHYP+VnURWPalladioL FontSubset: true FirstChar: 161 LastChar: 226 FontDescriptor: FontName: DOMHYP+VnURWPalladioL Flags: Symbolic FontBBox: -166, -283, 1021, 943 FontFile: true EncodingDictionary: Differences: true Pages: Page: Sequence: 1 Annotations: Annotation: Subtype: Link Rect: 82, 323, 152, 332 Annotation: Subtype: Link Rect: 34, 312, 99, 320 Annotation: Subtype: Link Rect: 34, 164, 96, 186 Annotation: Subtype: Link Rect: 126, 90, 154, 99 Annotation: Subtype: Link Rect: 34, 78, 147, 87 Annotation: Subtype: Link Rect: 34, 756, 169, 792 Annotation: Subtype: Link Rect: 515, 756, 560, 792 Annotation: Subtype: Link Rect: 253, 642, 264, 655 Annotation: Subtype: Link Rect: 383, 642, 394, 655 Annotation: Subtype: Link Rect: 131, 629, 143, 643 Annotation: Subtype: Link Rect: 365, 629, 376, 643 Annotation: Subtype: Link Rect: 269, 181, 276, 189 ActionDest: 190 Annotation: Subtype: Link Rect: 198, 155, 205, 164 ActionDest: 190 Annotation: Subtype: Link Rect: 403, 143, 410, 152 ActionDest: 190 Annotation: Subtype: Link Rect: 225, 105, 232, 114 ActionDest: 190 Annotation: Subtype: Link Rect: 542, 93, 549, 102 ActionDest: 194 Annotation: Subtype: Link Rect: 120, 17, 254, 28 Annotation: Subtype: Link Rect: 404, 17, 560, 28 Annotation: Subtype: Link Rect: 34, 432, 87, 451 Page: Sequence: 2 Annotations: Annotation: Subtype: Link Rect: 322, 735, 329, 744 ActionDest: 194 Annotation: Subtype: Link Rect: 247, 660, 254, 669 ActionDest: 194 Annotation: Subtype: Link Rect: 415, 560, 422, 569 ActionDest: 194 Annotation: Subtype: Link Rect: 519, 522, 526, 531 ActionDest: 194 Annotation: Subtype: Link Rect: 187, 447, 194, 456 ActionDest: 194 Annotation: Subtype: Link Rect: 306, 194, 318, 203 ActionDest: 194 Annotation: Subtype: Link Rect: 212, 81, 223, 90 ActionDest: 194 Page: Sequence: 3 Annotations: Annotation: Subtype: Link Rect: 520, 572, 532, 581 ActionDest: 194 Page: Sequence: 4 Annotations: Annotation: Subtype: Link Rect: 380, 660, 392, 669 ActionDest: 194 Annotation: Subtype: Link Rect: 281, 622, 293, 631 ActionDest: 194 Annotation: Subtype: Link Rect: 293, 622, 305, 631 ActionDest: 194 Annotation: Subtype: Link Rect: 463, 480, 470, 492 ActionDest: 84 Annotation: Subtype: Link Rect: 237, 231, 244, 243 ActionDest: 84 Page: Sequence: 5 Annotations: Annotation: Subtype: Link Rect: 501, 545, 508, 557 ActionDest: 84 Annotation: Subtype: Link Rect: 332, 520, 339, 532 ActionDest: 94 Annotation: Subtype: Link Rect: 410, 483, 417, 495 ActionDest: 94 Page: Sequence: 6 Page: Sequence: 7 Annotations: Annotation: Subtype: Link Rect: 267, 717, 274, 729 ActionDest: 84 Annotation: Subtype: Link Rect: 416, 680, 423, 692 ActionDest: 126 Annotation: Subtype: Link Rect: 426, 680, 433, 692 ActionDest: 167 Annotation: Subtype: Link Rect: 406, 667, 413, 679 ActionDest: 84 Page: Sequence: 8 Annotations: Annotation: Subtype: Link Rect: 279, 181, 286, 193 ActionDest: 147 Page: Sequence: 9 Annotations: Annotation: Subtype: Link Rect: 515, 64, 527, 73 ActionDest: 194 Page: Sequence: 10 Annotations: Annotation: Subtype: Link Rect: 267, 723, 279, 732 ActionDest: 194 Annotation: Subtype: Link Rect: 360, 685, 372, 694 ActionDest: 194 Annotation: Subtype: Link Rect: 416, 660, 428, 669 ActionDest: 194 Annotation: Subtype: Link Rect: 376, 622, 388, 631 ActionDest: 194 Annotation: Subtype: Link Rect: 391, 623, 403, 631 ActionDest: 194 Annotation: Subtype: Link Rect: 479, 610, 491, 619 ActionDest: 194 Annotation: Subtype: Link Rect: 422, 522, 429, 531 ActionDest: 194 Annotation: Subtype: Link Rect: 430, 522, 442, 531 ActionDest: 194 Annotation: Subtype: Link Rect: 502, 434, 514, 443 ActionDest: 194 Annotation: Subtype: Link Rect: 227, 384, 234, 393 ActionDest: 194 Annotation: Subtype: Link Rect: 234, 384, 246, 393 ActionDest: 194 Annotation: Subtype: Link Rect: 373, 359, 385, 368 ActionDest: 194 Annotation: Subtype: Link Rect: 205, 309, 217, 318 ActionDest: 194 Annotation: Subtype: Link Rect: 362, 296, 374, 305 ActionDest: 194 Annotation: Subtype: Link Rect: 469, 283, 481, 293 ActionDest: 194 Annotation: Subtype: Link Rect: 512, 208, 524, 217 ActionDest: 194 Annotation: Subtype: Link Rect: 230, 120, 242, 129 ActionDest: 194 Page: Sequence: 11 Annotations: Annotation: Subtype: Link Rect: 189, 735, 201, 744 ActionDest: 194 Annotation: Subtype: Link Rect: 202, 735, 214, 744 ActionDest: 194 Annotation: Subtype: Link Rect: 274, 698, 286, 707 ActionDest: 194 Annotation: Subtype: Link Rect: 192, 647, 204, 657 ActionDest: 197 Annotation: Subtype: Link Rect: 355, 610, 367, 619 ActionDest: 197 Annotation: Subtype: Link Rect: 432, 572, 444, 581 ActionDest: 197 Annotation: Subtype: Link Rect: 318, 535, 330, 544 ActionDest: 197 Annotation: Subtype: Link Rect: 424, 445, 560, 456 Annotation: Subtype: Link Rect: 165, 433, 263, 444 Annotation: Subtype: Link Rect: 476, 106, 513, 117 Annotation: Subtype: Link Rect: 520, 106, 556, 117 Annotation: Subtype: Link Rect: 453, 83, 490, 94 Annotation: Subtype: Link Rect: 496, 83, 532, 94 Annotation: Subtype: Link Rect: 59, 61, 96, 71 Page: Sequence: 12 Annotations: Annotation: Subtype: Link Rect: 59, 722, 96, 733 Annotation: Subtype: Link Rect: 340, 699, 377, 710 Annotation: Subtype: Link Rect: 383, 699, 419, 710 Annotation: Subtype: Link Rect: 95, 676, 133, 687 Annotation: Subtype: Link Rect: 182, 653, 220, 664 Annotation: Subtype: Link Rect: 59, 630, 96, 640 Annotation: Subtype: Link Rect: 102, 630, 138, 640 Annotation: Subtype: Link Rect: 520, 606, 557, 617 Annotation: Subtype: Link Rect: 288, 583, 325, 594 Annotation: Subtype: Link Rect: 339, 572, 560, 583 Annotation: Subtype: Link Rect: 56, 560, 258, 571 Annotation: Subtype: Link Rect: 164, 538, 202, 548 Annotation: Subtype: Link Rect: 208, 538, 244, 548 Annotation: Subtype: Link Rect: 433, 526, 470, 537 Annotation: Subtype: Link Rect: 252, 504, 290, 514 Annotation: Subtype: Link Rect: 296, 504, 332, 514 Annotation: Subtype: Link Rect: 451, 480, 488, 491 Annotation: Subtype: Link Rect: 495, 480, 531, 491 Annotation: Subtype: Link Rect: 59, 458, 96, 468 Annotation: Subtype: Link Rect: 102, 458, 138, 468 Annotation: Subtype: Link Rect: 496, 445, 533, 456 Annotation: Subtype: Link Rect: 212, 412, 250, 422 Annotation: Subtype: Link Rect: 59, 377, 96, 387 Annotation: Subtype: Link Rect: 140, 354, 177, 364 Annotation: Subtype: Link Rect: 131, 331, 168, 341 Annotation: Subtype: Link Rect: 59, 297, 96, 307 Annotation: Subtype: Link Rect: 102, 297, 138, 307 Annotation: Subtype: Link Rect: 519, 273, 557, 284 Annotation: Subtype: Link Rect: 150, 251, 187, 261 Annotation: Subtype: Link Rect: 194, 251, 230, 261 Annotation: Subtype: Link Rect: 467, 227, 505, 238 Annotation: Subtype: Link Rect: 511, 227, 547, 238 Annotation: Subtype: Link Rect: 358, 204, 395, 215 Annotation: Subtype: Link Rect: 311, 182, 349, 192 Annotation: Subtype: Link Rect: 408, 158, 445, 169 Annotation: Subtype: Link Rect: 289, 135, 326, 146 Annotation: Subtype: Link Rect: 183, 101, 221, 111 Annotation: Subtype: Link Rect: 375, 77, 413, 88 Page: Sequence: 13 Annotations: Annotation: Subtype: Link Rect: 187, 722, 224, 733 Annotation: Subtype: Link Rect: 231, 722, 267, 733 Annotation: Subtype: Link Rect: 258, 687, 295, 698 Annotation: Subtype: Link Rect: 301, 687, 337, 698 Annotation: Subtype: Link Rect: 118, 665, 155, 675 Annotation: Subtype: Link Rect: 416, 641, 454, 652 Annotation: Subtype: Link Rect: 460, 641, 496, 652 Checksum: d75a14a4 Type: CRC32 Checksum: 836b51f517e9a5626fc32c59c1ec5be6 Type: MD5 Checksum: f9c0f3d7b5856a65a5d477598aee7fb76cc8f689 Type: SHA-1