8cd878ab3835bfa21090a2d63d40b5bb ANIM_a2022v12p3417.pdf f3c4c5c29e3d9621af2dab6e7ee3e0ab411bd922 ANIM_a2022v12p3417.pdf bdf7aebc3b3184fb057c2fced3b474fae9c434e1b436271951aec827f4d7a476 ANIM_a2022v12p3417.pdf Title: ProAKAP4 Concentration Is Related to Sperm Motility and Motile Sperm Subpopulations in Frozen–Thawed Horse Semen Subject: ProAKAP4 is the precursor of AKAP4 (A-kinase Anchor protein 4), the main structural protein of the fibrous sheath of sperm. The amount of proAKAP4 reflects the ability of spermatozoa to maintain the flagellum activity and functionality up to the site of fertilization and is positively correlated with progressive motility in several mammalian species. The aim of this study was to investigate the relationship between proAKAP4 concentration with horse sperm motility descriptors and spermatic motile subpopulations. For this purpose, a total of 48 ejaculates from 13 different stallions were analyzed. Spermatic motility descriptors were obtained by the CASA system, and four motile subpopulations (SP) with specific motility patterns were statistically identified. ProAKAP4 concentrations were evaluated by ELISA. The relationship between motility descriptors of sperm subpopulations and proAKAP4 concentrations was evaluated. Following a hierarchical cluster statistical analysis, ejaculates were divided into two groups according to their proAKAP4 concentrations, either having low proAKAP4 concentrations (5.06–35.61 ng/10M spz; n = 23) or high (39.92–82.23 ng/10M spz; n = 25) proAKAP4 concentrations (p < 0.001). ProAKAP4 concentrations were positively correlated (p < 0.05) with total and progressive motility, as well as with parameters of velocity. ProAKAP4 amount also showed a negative correlation (p < 0.05) with sperm motile subpopulation number 3, which was the subpopulation with the lowest velocity parameters. In conclusion, proAKAP4 concentration in stallion semen positively reflects sperm progressive motility with the functional velocity kinematic descriptors. Concentrations of proAKAP4 higher than 37.77 ng/10M spz were correlated with a very good quality frozen/thawed stallion semen. Keywords: ProAKAP4; AKAP4; stallion; spermatozoa; spermatic subpopulations Author: Marta Dordas-Perpinyà, Ivan Yanez-Ortiz, Nicolas Sergeant, Vincent Mevel, Jean-François Bruyas, Jaime Catalán, Maryse Delehedde, Lamia Briand-Amirat and Jordi Miró Creator: LaTeX with hyperref Producer: pdfTeX-1.40.21 CreationDate: Mon Dec 5 04:21:25 2022 CET ModDate: Mon Dec 5 04:25:22 2022 CET Tagged: no UserProperties: no Suspects: no Form: none JavaScript: no Pages: 11 Encrypted: no Page size: 595.276 x 841.89 pts (A4) Page rot: 0 File size: 1666396 bytes Optimized: no PDF version: 1.7 name type encoding emb sub uni object ID ------------------------------------ ----------------- ---------------- --- --- --- --------- IBTYZX+VnURWPalladioL Type 1 Custom yes yes no 10 0 BZLYOC+URWPalladioL-Bold Type 1 Custom yes yes no 15 0 NMTGDA+URWPalladioL-Roma Type 1 Custom yes yes no 20 0 OLITHJ+URWPalladioL-Ital Type 1 Custom yes yes no 24 0 NEBEKD+VnURWPalladioL-Bold Type 1 Custom yes yes no 28 0 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BCDJEE+PalatinoLinotype-Italic CID TrueType Identity-H yes yes yes 164 0 BCDEEE+PalatinoLinotype-Bold TrueType WinAnsi yes yes no 181 0 BCDFEE+PalatinoLinotype-Roman TrueType WinAnsi yes yes no 185 0 BCDGEE+PalatinoLinotype-Italic TrueType WinAnsi yes yes no 189 0 BCDIEE+PalatinoLinotype-Roman CID TrueType Identity-H yes yes yes 193 0 BCDJEE+PalatinoLinotype-Italic CID TrueType Identity-H yes yes yes 199 0 Jhove (Rel. 1.22.1, 2019-04-17) Date: 2023-01-24 02:35:31 CET RepresentationInformation: ANIM_a2022v12p3417.pdf ReportingModule: PDF-hul, Rel. 1.12.1 (2019-04-17) LastModified: 2023-01-23 11:55:39 CET Size: 1666396 Format: PDF Version: 1.7 Status: Well-Formed and valid SignatureMatches: PDF-hul MIMEtype: application/pdf PDFMetadata: Objects: 401 FreeObjects: 1 IncrementalUpdates: 0 DocumentCatalog: PageLayout: SinglePage PageMode: UseNone Outlines: Item: Title: Introduction Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@4923ab24 Item: Title: Materials and Methods Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@44c8afef Children: Item: Title: Semen Collection and Cryopreservation Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@7b69c6ba Item: Title: Sperm Cryopreservation Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@46daef40 Item: Title: Computerized Assisted Semen Analysis Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@12f41634 Item: Title: proAKAP4 ELISA Assays Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@13c27452 Item: Title: Statistical Analysis Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@262b2c86 Item: Title: Results Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@371a67ec Children: Item: Title: Classification of Frozen Thawed Horse Ejaculates according to proAKAP4 Concentration Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@5ed828d Item: Title: Relationship between proAKAP4 Groups and Its Distribution in Different Breeds Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@50d0686 Item: Title: Relationship between proAKAP4 Concentration and Sperm Motility and Motile Sperm Subpopulations in Frozen Thawed Horse Ejaculates Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@7a3d45bd Item: Title: Correlation between proAKAP4 Concentration with Sperm Motility and Motile Sperm Subpopulations of Frozen Thawed Horse Ejaculates Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@1e7c7811 Item: Title: Discussion Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@77ec78b9 Item: Title: Conclusions Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@1a3869f4 Item: Title: References Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@a38d7a3 Info: Title: ProAKAP4 Concentration Is Related to Sperm Motility and Motile Sperm Subpopulations in Frozen Thawed Horse Semen Author: Marta Dordas-Perpinyà, Ivan Yanez-Ortiz, Nicolas Sergeant, Vincent Mevel, Jean-François Bruyas, Jaime Catalán, Maryse Delehedde, Lamia Briand-Amirat and Jordi Miró Subject: ProAKAP4 is the precursor of AKAP4 (A-kinase Anchor protein 4), the main structural protein of the fibrous sheath of sperm. The amount of proAKAP4 reflects the ability of spermatozoa to maintain the flagellum activity and functionality up to the site of fertilization and is positively correlated with progressive motility in several mammalian species. The aim of this study was to investigate the relationship between proAKAP4 concentration with horse sperm motility descriptors and spermatic motile subpopulations. For this purpose, a total of 48 ejaculates from 13 different stallions were analyzed. Spermatic motility descriptors were obtained by the CASA system, and four motile subpopulations (SP) with specific motility patterns were statistically identified. ProAKAP4 concentrations were evaluated by ELISA. The relationship between motility descriptors of sperm subpopulations and proAKAP4 concentrations was evaluated. Following a hierarchical cluster statistical analysis, ejaculates were divided into two groups according to their proAKAP4 concentrations, either having low proAKAP4 concentrations (5.06 35.61 ng/10M spz; n = 23) or high (39.92 82.23 ng/10M spz; n = 25) proAKAP4 concentrations (p < 0.001). ProAKAP4 concentrations were positively correlated (p < 0.05) with total and progressive motility, as well as with parameters of velocity. ProAKAP4 amount also showed a negative correlation (p < 0.05) with sperm motile subpopulation number 3, which was the subpopulation with the lowest velocity parameters. In conclusion, proAKAP4 concentration in stallion semen positively reflects sperm progressive motility with the functional velocity kinematic descriptors. Concentrations of proAKAP4 higher than 37.77 ng/10M spz were correlated with a very good quality frozen/thawed stallion semen. 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