e5a5c3b4e222066d43ca6442b9344f44 PAT_a2022v11p1451.pdf 83231688da26eff418c8b8fc3251f5f6c19fd01b PAT_a2022v11p1451.pdf e6bbec6e0925e32716c68ffe688c5a85edb8da094e15d9bd7b4012eec8e3a434 PAT_a2022v11p1451.pdf Title: Lack of Detection of Toxoplasma gondii in Pipistrellus spp. Bats from Densely Cat-Populated Areas of NE Spain Subject: Toxoplasma gondii infection in healthy animals is often asymptomatic. However, some species with little history of contact with the parasite, such as marsupials and New World primates, present high mortality rates after infection. Despite its potential conservation concern, T. gondii infection in insectivorous bats has received little attention, and its impact on bat populations’ health is unknown. To assess the putative role of insectivorous bats in the cycle of T. gondii, samples of three species of bats (Pipistrellus pipistrellus, P. pygmaeus and P. kuhlii) collected between 2019 and 2021 in NE Spain were tested for the presence of the parasite using a qPCR. All tissues resulted negative (0.0% prevalence with 95% CI: [0.0–2.6]) for the presence of T. gondii. Unlike previous studies on insectivorous bats from Europe, Asia and America, the present study suggests that Pipistrellus spp. bats do not play a significant role in the epidemiology of T. gondii in NE Spain. Further studies are encouraged to elucidate both the epidemiology of T. gondii and its potential impact on the health of microchiropteran species in Europe. Keywords: Chiroptera; Vespertilionidae; wildlife; diseases; host–pathogen interactions; health; Sarcocystidae; Toxoplasma gondii Author: Lourdes Lobato-Bailón, Ane López-Morales, Rita Quintela, Maria Puig Ribas, Rafael Molina-López, Elena Obon, Sebastián Napp, Lola Pailler-García, Johan Espunyes and Óscar Cabezón Creator: LaTeX with hyperref Producer: pdfTeX-1.40.21 CreationDate: Mon Dec 5 03:54:07 2022 CET ModDate: Mon Dec 5 03:57:29 2022 CET Tagged: no UserProperties: no Suspects: no Form: none JavaScript: no Pages: 7 Encrypted: no Page size: 595.276 x 841.89 pts (A4) Page rot: 0 File size: 634152 bytes Optimized: no PDF version: 1.7 name type encoding emb sub uni object ID ------------------------------------ ----------------- ---------------- --- --- --- --------- QTXWOK+VnURWPalladioL Type 1 Custom yes yes no 10 0 UOGZQF+URWPalladioL-Bold Type 1 Custom yes yes no 15 0 EAAFWN+URWPalladioL-Roma Type 1 Custom yes yes no 20 0 GYFOFX+URWPalladioL-Ital Type 1 Custom yes yes no 24 0 UOXYDI+URWPalladioL-BoldItal Type 1 Custom yes yes no 28 0 OKMZCJ+VnURWPalladioL-Bold Type 1 Custom yes yes no 32 0 UXHOAG+TimesNewRomanPS-BoldItalicMT Type 1C WinAnsi yes yes no 51 0 AAPCWM+CMSY10 Type 1 Builtin yes yes no 77 0 ZXDKIP+EURM10 Type 1 Builtin yes yes no 81 0 BCDEEE+PalatinoLinotype-Bold TrueType WinAnsi yes yes no 89 0 BCDFEE+PalatinoLinotype-Roman TrueType WinAnsi yes yes no 93 0 BCDGEE+PalatinoLinotype-Italic TrueType WinAnsi yes yes no 97 0 BCDIEE+PalatinoLinotype-Roman CID TrueType Identity-H yes yes yes 101 0 IOLAWT+PazoMath-Italic Type 1 Builtin yes yes no 112 0 JHYTSG+CMR10 Type 1 Builtin yes yes no 116 0 CRUZTT+VnURWPalladioL-Italic Type 1 Custom yes yes no 126 0 Jhove (Rel. 1.22.1, 2019-04-17) Date: 2023-02-02 02:37:16 CET RepresentationInformation: PAT_a2022v11p1451.pdf ReportingModule: PDF-hul, Rel. 1.12.1 (2019-04-17) LastModified: 2023-02-01 12:48:47 CET Size: 634152 Format: PDF Version: 1.7 Status: Well-Formed and valid SignatureMatches: PDF-hul MIMEtype: application/pdf PDFMetadata: Objects: 277 FreeObjects: 1 IncrementalUpdates: 0 DocumentCatalog: PageLayout: SinglePage PageMode: UseNone Outlines: Item: Title: Introduction Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@4923ab24 Item: Title: Materials and Methods Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@44c8afef Children: Item: Title: Animals and Samples Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@7b69c6ba Item: Title: Molecular Analysis Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@46daef40 Item: Title: Statistical Analyses Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@12f41634 Item: Title: Results Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@13c27452 Item: Title: Discussion Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@262b2c86 Item: Title: Conclusions Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@371a67ec Item: Title: References Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@5ed828d Info: Title: Lack of Detection of Toxoplasma gondii in Pipistrellus spp. Bats from Densely Cat-Populated Areas of NE Spain Author: Lourdes Lobato-Bailón, Ane López-Morales, Rita Quintela, Maria Puig Ribas, Rafael Molina-López, Elena Obon, Sebastián Napp, Lola Pailler-García, Johan Espunyes and Óscar Cabezón Subject: Toxoplasma gondii infection in healthy animals is often asymptomatic. However, some species with little history of contact with the parasite, such as marsupials and New World primates, present high mortality rates after infection. Despite its potential conservation concern, T. gondii infection in insectivorous bats has received little attention, and its impact on bat populations’ health is unknown. To assess the putative role of insectivorous bats in the cycle of T. gondii, samples of three species of bats (Pipistrellus pipistrellus, P. pygmaeus and P. kuhlii) collected between 2019 and 2021 in NE Spain were tested for the presence of the parasite using a qPCR. All tissues resulted negative (0.0% prevalence with 95% CI: [0.0 2.6]) for the presence of T. gondii. Unlike previous studies on insectivorous bats from Europe, Asia and America, the present study suggests that Pipistrellus spp. bats do not play a significant role in the epidemiology of T. gondii in NE Spain. Further studies are encouraged to elucidate both the epidemiology of T. gondii and its potential impact on the health of microchiropteran species in Europe. 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