97bf06c3b4d58dc44db94c0c96307e95 ANL_a2022v12p3525.pdf 4a06e3c0c10c9013c807df024a73e0c7878d0a58 ANL_a2022v12p3525.pdf 1ee9a1f29fbf9ac5a92f61b061e4cb057026d7fe83eaddf926035bba67c954c8 ANL_a2022v12p3525.pdf Title: Short Communication: Evaluation of Intestinal Release of Butyric Acid from Sodium Butyrate Protected by Salts of Medium-Chain Fatty Acids in Broiler Chickens Subject: Butyric acid has received great attention as a feed additive to maintain or increase the gut integrity and health of broiler chickens. Particularly, the protection of butyrate is under research to allow slow intestinal release of butyric acid and to promote its beneficial effects throughout the intestine. This study evaluated in vivo the intestinal release of butyric acid from sodium butyrate protected by salts of medium-chain fatty acid in broilers. Brilliant blue was used as an inert marker, so it was included in the feed additive that broilers ingested for two days. The gastrointestinal tract was then colored in blue from jejunum and backward. Considering the digesta color of the broilers non-supplemented as blank, it allowed quantification of the amount of brilliant blue, and consequently, butyric acid delivered in the intestine from the protected feed additive. Few traces of butyric acid were released in the duodenum and proximal jejunum, whereas the major amount (45.9%) was delivered in the distal ileum (p < 0.001). These results suggest that this in vivo approach allows for evaluation of the intestinal delivery of butyric acid supplemented as protected sodium butyrate by medium-chain fatty acids, showing a gradual intestinal release of butyric acid in broiler chickens. Keywords: butyric acid; protected butyrate; medium-chain fatty acid; feed additive; gut health; intestinal release; broiler Author: Meritxell Sadurní, Ana Cristina Barroeta, Cinta Sol, Mónica Puyalto and Lorena Castillejos Creator: LaTeX with hyperref Producer: pdfTeX-1.40.21 CreationDate: Tue Dec 20 04:51:32 2022 CET ModDate: Tue Dec 20 04:54:17 2022 CET Tagged: no UserProperties: no Suspects: no Form: none JavaScript: no Pages: 7 Encrypted: no Page size: 595.276 x 841.89 pts (A4) Page rot: 0 File size: 475257 bytes Optimized: no PDF version: 1.7 name type encoding emb sub uni object ID ------------------------------------ ----------------- ---------------- --- --- --- --------- QBLEMJ+VnURWPalladioL Type 1 Custom yes yes no 10 0 GIOGVN+URWPalladioL-Bold Type 1 Custom yes yes no 15 0 NPAQEQ+URWPalladioL-Roma Type 1 Custom yes yes no 20 0 DJYSAQ+URWPalladioL-Ital Type 1 Custom yes yes no 24 0 YWWVQQ+VnURWPalladioL-Bold Type 1 Custom yes yes no 28 0 SZMRXJ+TimesNewRomanPS-BoldItalicMT Type 1C WinAnsi yes yes no 46 0 MDJHIH+CMSY10 Type 1 Builtin yes yes no 71 0 IIICMB+PalatinoLinotype,Bold TrueType WinAnsi yes yes no 85 0 IIICMD+PalatinoLinotype TrueType WinAnsi yes yes no 88 0 IIICMF+PalatinoLinotype,Italic TrueType WinAnsi yes yes no 91 0 WBVKDJ+URWPalladioL-BoldItal Type 1 Custom yes yes no 99 0 LWLIVD+EURM10 Type 1 Builtin yes yes no 106 0 NJWNDJ+VnURWPalladioL-Italic Type 1 Custom yes yes no 110 0 Jhove (Rel. 1.22.1, 2019-04-17) Date: 2023-01-25 02:30:47 CET RepresentationInformation: ANL_a2022v12p3525.pdf ReportingModule: PDF-hul, Rel. 1.12.1 (2019-04-17) LastModified: 2023-01-24 13:09:53 CET Size: 475257 Format: PDF Version: 1.7 Status: Well-Formed and valid SignatureMatches: PDF-hul MIMEtype: application/pdf PDFMetadata: Objects: 205 FreeObjects: 1 IncrementalUpdates: 0 DocumentCatalog: PageLayout: SinglePage PageMode: UseNone Outlines: Item: Title: Introduction Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@4923ab24 Item: Title: Materials and Methods Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@44c8afef Children: Item: Title: Housing, Animals, and Experimental Design Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@7b69c6ba Item: Title: Sampling and Analytical Determinations Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@46daef40 Item: Title: Statistical Analysis Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@12f41634 Item: Title: Results and Discussion Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@13c27452 Item: Title: Conclusions Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@262b2c86 Item: Title: References Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@371a67ec Info: Title: Short Communication: Evaluation of Intestinal Release of Butyric Acid from Sodium Butyrate Protected by Salts of Medium-Chain Fatty Acids in Broiler Chickens Author: Meritxell Sadurní, Ana Cristina Barroeta, Cinta Sol, Mónica Puyalto and Lorena Castillejos Subject: Butyric acid has received great attention as a feed additive to maintain or increase the gut integrity and health of broiler chickens. Particularly, the protection of butyrate is under research to allow slow intestinal release of butyric acid and to promote its beneficial effects throughout the intestine. This study evaluated in vivo the intestinal release of butyric acid from sodium butyrate protected by salts of medium-chain fatty acid in broilers. Brilliant blue was used as an inert marker, so it was included in the feed additive that broilers ingested for two days. The gastrointestinal tract was then colored in blue from jejunum and backward. Considering the digesta color of the broilers non-supplemented as blank, it allowed quantification of the amount of brilliant blue, and consequently, butyric acid delivered in the intestine from the protected feed additive. Few traces of butyric acid were released in the duodenum and proximal jejunum, whereas the major amount (45.9%) was delivered in the distal ileum (p < 0.001). These results suggest that this in vivo approach allows for evaluation of the intestinal delivery of butyric acid supplemented as protected sodium butyrate by medium-chain fatty acids, showing a gradual intestinal release of butyric acid in broiler chickens. 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