8640bf57b28fe09176f4fd763930ed40 CEL_a2022v11p4074.pdf 8aca548ca9d70ce0dd7db57dda45ee55b2332a19 CEL_a2022v11p4074.pdf 8f63288442dd48b85f56633f75e1b53f8fe68c447c46bff76f2239efce11ae4d CEL_a2022v11p4074.pdf Title: Repair of Long Nerve Defects with a New Decellularized Nerve Graft in Rats and in Sheep Subject: Decellularized nerve allografts (DC) are an alternative to autografts (AG) for repairing severe peripheral nerve injuries. We have assessed a new DC provided by VERIGRAFT. The decellularization procedure completely removed cellularity while preserving the extracellular matrix. We first assessed the DC in a 15 mm gap in the sciatic nerve of rats, showing slightly delayed but effective regeneration. Then, we assayed the DC in a 70 mm gap in the peroneal nerve of sheep compared with AG. Evaluation of nerve regeneration and functional recovery was performed by clinical, electrophysiology and ultrasound tests. No significant differences were found in functional recovery between groups of sheep. Histology showed a preserved fascicular structure in the AG while in the DC grafts regenerated axons were grouped in small units. In conclusion, the DC was permissive for axonal regeneration and allowed to repair a 70 mm long gap in the sheep nerve. Keywords: nerve regeneration; autograft; allograft; decellularization; sheep Author: Estefanía Contreras, Sara Traserra, Sara Bolívar, Joaquim Forés, Eduard Jose-Cunilleras, Felix García, Ignacio Delgado-Martínez, Sandra Holmgren, Raimund Strehl, Esther Udina and Xavier Navarro Creator: LaTeX with hyperref Producer: pdfTeX-1.40.21 CreationDate: Sun Dec 18 03:16:38 2022 CET ModDate: Sun Dec 18 03:24:47 2022 CET Tagged: no UserProperties: no Suspects: no Form: none JavaScript: no Pages: 19 Encrypted: no Page size: 595.276 x 841.89 pts (A4) Page rot: 0 File size: 5249496 bytes Optimized: no PDF version: 1.7 name type encoding emb sub uni object ID ------------------------------------ ----------------- ---------------- --- --- --- --------- UOHDZX+VnURWPalladioL Type 1 Custom yes yes no 10 0 EVRVJM+URWPalladioL-Bold Type 1 Custom yes yes no 15 0 GEDKHI+URWPalladioL-Roma Type 1 Custom yes yes no 20 0 CTHCWQ+URWPalladioL-Ital Type 1 Custom yes yes no 24 0 YSPQJI+VnURWPalladioL-Bold Type 1 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Well-Formed and valid SignatureMatches: PDF-hul MIMEtype: application/pdf PDFMetadata: Objects: 501 FreeObjects: 1 IncrementalUpdates: 0 DocumentCatalog: PageLayout: SinglePage PageMode: UseNone Outlines: Item: Title: Introduction Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@44c8afef Item: Title: Materials and Methods Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@7b69c6ba Children: Item: Title: Nerve Decellularization Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@46daef40 Item: Title: Rat Study Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@12f41634 Item: Title: Sheep Study Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@13c27452 Item: Title: Surgical Procedure Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@262b2c86 Item: Title: Functional Tests Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@371a67ec Item: Title: Electrophysiological Tests Destination: 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Test Results Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@3aeaafa6 Item: Title: Echographic Evaluation of TA Muscles Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@76a3e297 Item: Title: Histological Assessment Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@4d3167f4 Item: Title: Histological Evaluation of Reinnervated Targets Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@ed9d034 Item: Title: Discussion Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@6121c9d6 Children: Item: Title: Nerve Regeneration in the Sheep Peroneal Nerve Model Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@87f383f Item: Title: Regenerative Potential of the Acellular Nerve Graft Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@4eb7f003 Item: Title: References Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@eafc191 Info: Title: Repair of Long Nerve Defects with a New Decellularized Nerve Graft in Rats and in Sheep Author: Estefanía Contreras, Sara Traserra, Sara Bolívar, Joaquim Forés, Eduard Jose-Cunilleras, Felix García, Ignacio Delgado-Martínez, Sandra Holmgren, Raimund Strehl, Esther Udina and Xavier Navarro Subject: Decellularized nerve allografts (DC) are an alternative to autografts (AG) for repairing severe peripheral nerve injuries. We have assessed a new DC provided by VERIGRAFT. The decellularization procedure completely removed cellularity while preserving the extracellular matrix. We first assessed the DC in a 15 mm gap in the sciatic nerve of rats, showing slightly delayed but effective regeneration. Then, we assayed the DC in a 70 mm gap in the peroneal nerve of sheep compared with AG. Evaluation of nerve regeneration and functional recovery was performed by clinical, electrophysiology and ultrasound tests. No significant differences were found in functional recovery between groups of sheep. Histology showed a preserved fascicular structure in the AG while in the DC grafts regenerated axons were grouped in small units. In conclusion, the DC was permissive for axonal regeneration and allowed to repair a 70 mm long gap in the sheep nerve. 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