c3bd1e9bdb168b257c89c65186836bb5 270530.pdf be3ddb77c87e494ad6b195176227585d7b7a396e 270530.pdf 345e41c07f9866ba3108b98d9dd75b1a0798f312423fe4a48f983ad2efd3639c 270530.pdf Title: How Lineage Tracing Studies Can Unveil Tumor Heterogeneity in Breast Cancer Subject: Lineage tracing studies have become a well-suited approach to reveal cellular hierarchies and tumor heterogeneity. Cellular heterogeneity, particularly in breast cancer, is still one of the main concerns regarding tumor progression and resistance to anti-cancer therapies. Here, we review the current knowledge about lineage tracing analyses that have contributed to an improved comprehension of the complexity of mammary tumors, highlighting how targeting different mammary epithelial cells and tracing their progeny can be useful to explore the intra- and inter-heterogeneity observed in breast cancer. In addition, we examine the strategies used to identify the cell of origin in different breast cancer subtypes and summarize how cellular plasticity plays an important role during tumorigenesis. Finally, we evaluate the clinical implications of lineage tracing studies and the challenges remaining to address tumor heterogeneity in breast cancer. Keywords: breast cancer; lineage tracing; heterogeneity; cellular plasticity Author: Elena Vinuesa-Pitarch, Daniel Ortega-Álvarez and Verónica Rodilla Creator: LaTeX with hyperref Producer: pdfTeX-1.40.21 CreationDate: Tue Dec 21 10:23:53 2021 CET ModDate: Tue Dec 21 10:26:38 2021 CET Tagged: no UserProperties: no Suspects: no Form: none JavaScript: no Pages: 15 Encrypted: no Page size: 595.276 x 841.89 pts (A4) Page rot: 0 File size: 1598876 bytes Optimized: no PDF version: 1.7 name type encoding emb sub uni object ID ------------------------------------ ----------------- ---------------- --- --- --- --------- HCFYFJ+VnURWPalladioL-Bold Type 1 Custom yes yes no 10 0 YNMABH+VnURWPalladioL Type 1 Custom yes yes no 15 0 DBAXAG+URWPalladioL-Bold Type 1 Custom yes yes no 19 0 CMJOSL+URWPalladioL-Roma Type 1 Custom yes yes no 24 0 KILWVS+URWPalladioL-Ital Type 1 Custom yes yes no 28 0 QDCWHF+EURM10 Type 1 Builtin yes yes no 32 0 MCBCCI+SourceSansRoman-Regular Type 1C Custom yes yes no 40 0 MCBCCM+SourceSansRoman.613wght Type 1C Custom yes yes no 44 0 MCBCCO+SourceSansRoman-Bold Type 1C Custom yes yes no 48 0 EBFBTV+TimesNewRomanPS-BoldItalicMT Type 1C WinAnsi yes yes no 60 0 KNLDBB+PalatinoLinotype,Bold TrueType WinAnsi yes yes no 82 0 KNLDBC+PalatinoLinotype CID TrueType Identity-H yes yes yes 85 0 KNLDBD+PalatinoLinotype TrueType WinAnsi yes yes no 91 0 KNLDCF+PalatinoLinotype,Italic TrueType WinAnsi yes yes no 94 0 KNLDBB+PalatinoLinotype,Bold TrueType WinAnsi yes yes no 108 0 KNLDBC+PalatinoLinotype CID TrueType Identity-H yes yes yes 111 0 KNLDBD+PalatinoLinotype TrueType WinAnsi yes yes no 117 0 KNLDCF+PalatinoLinotype,Italic TrueType WinAnsi yes yes no 120 0 KNLDBB+PalatinoLinotype,Bold TrueType WinAnsi yes yes no 130 0 KNLDBC+PalatinoLinotype CID TrueType Identity-H yes yes yes 133 0 KNLDBD+PalatinoLinotype TrueType WinAnsi yes yes no 139 0 KNLDCF+PalatinoLinotype,Italic TrueType WinAnsi yes yes no 142 0 FXXUVH+CMSY10 Type 1 Builtin yes yes no 155 0 KNLDBB+PalatinoLinotype,Bold TrueType WinAnsi yes yes no 171 0 KNLDBC+PalatinoLinotype CID TrueType Identity-H yes yes yes 174 0 KNLDBD+PalatinoLinotype TrueType WinAnsi yes yes no 180 0 KNLDCF+PalatinoLinotype,Italic TrueType WinAnsi yes yes no 183 0 Jhove (Rel. 1.22.1, 2019-04-17) Date: 2023-02-10 02:34:08 CET RepresentationInformation: 270530.pdf ReportingModule: PDF-hul, Rel. 1.12.1 (2019-04-17) LastModified: 2023-02-09 11:53:21 CET Size: 1598876 Format: PDF Version: 1.7 Status: Well-Formed and valid SignatureMatches: PDF-hul MIMEtype: application/pdf PDFMetadata: Objects: 584 FreeObjects: 1 IncrementalUpdates: 0 DocumentCatalog: PageLayout: SinglePage PageMode: UseNone Outlines: Item: Title: Introduction Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@44c8afef Item: Title: Lineage Tracing Is the Gold-Standard Approach for Exploring Cellular Hierarchies and Tumor Heterogeneity in Breast Cancer Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@7b69c6ba Children: Item: Title: Searching for the Tumor Cell of Origin in Different Breast Cancer Subtypes Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@46daef40 Item: Title: Importance of the Epithelial-to-Mesenchymal Transition during Tumor Progression Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@12f41634 Item: Title: Differential Clonal Expansion during Tumor Progression and the Metastatic Process Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@13c27452 Item: Title: In Vivo Models to Study Mammary Gland Tumorigenesis Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@262b2c86 Children: Item: Title: Classic Preclinical Models to Recapitulate Different Breast Cancer Subtypes Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@371a67ec Item: Title: Cellular Plasticity Plays an Important Role in the Development of Mammary Tumors Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@5ed828d Item: Title: Clinical Implications of Lineage Tracing Studies and Future Perspectives Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@50d0686 Item: Title: References Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@7a3d45bd Info: Title: How Lineage Tracing Studies Can Unveil Tumor Heterogeneity in Breast Cancer Author: Elena Vinuesa-Pitarch, Daniel Ortega-Álvarez and Verónica Rodilla Subject: Lineage tracing studies have become a well-suited approach to reveal cellular hierarchies and tumor heterogeneity. Cellular heterogeneity, particularly in breast cancer, is still one of the main concerns regarding tumor progression and resistance to anti-cancer therapies. Here, we review the current knowledge about lineage tracing analyses that have contributed to an improved comprehension of the complexity of mammary tumors, highlighting how targeting different mammary epithelial cells and tracing their progeny can be useful to explore the intra- and inter-heterogeneity observed in breast cancer. In addition, we examine the strategies used to identify the cell of origin in different breast cancer subtypes and summarize how cellular plasticity plays an important role during tumorigenesis. Finally, we evaluate the clinical implications of lineage tracing studies and the challenges remaining to address tumor heterogeneity in breast cancer. 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