This is the accepted version of the journal article: Okada, M.; Yang, Z.; Mas, P. «Circadian autonomy and rhythmic precision of the Arabidopsis female reproductive organ». Developmental Cell, Vol. 57 Núm. 18 (26 2022), p. 2168-2180.e4. DOI 10.1016/j.devcel.2022.08.013 This version is available at https://ddd.uab.cat/record/273537 under the terms of the license A YOUNG BUDS MATURE BUDS MATURE FLOWERS OPEN FLOWERS B C Relative Amplitude Error Relative luminescence D E 0.6 Relative luminescence Mature flowers Open flowers 0.4 0.2 0.0 16 20 24 28 32 Estimated Period (h) F G Relative gene expression Relative luminescence Relative luminescence Relative luminescence Relative Amplitude Error E A C G Relative luminescence Relative luminescence Relative Amplitude Error Relative Amplitude Error F H B D Relative Amplitude Error A B E H SEPAL Relative luminescence Relative Amplitude Error I F C PETAL STAMEN Relative Amplitude Error J D G PISTIL A B C I J K Stage11 Stage12 Stage13 250 3.5 800 25 Pistil WT Silique length (mm) CCA1-ox * 20 Pistil length (mm) 200 Silique number 3.0 600 *** *** 15 150 * 2.5 10 100 * 400 2.0 5 50 1.5 200 0 WT CCA1-ox WT CCA1-ox 0 0 2 4 6 8 10 12 14 16 18 20 22 24 L M Estimated Phase (h) WT CCA1-ox D N O P Seed sectional area (mm2) 0.022 1000-grain-weight (g) 0.020 *** E F 0.018 0.016 0.014 WT CCA1-ox Q Stage13 R Stage11 Stage12 Stage13 4.0 WT * Pistil length (mm) toc1-2 Pistil length (mm) 3.5 3.00 TOC1-ox ** 3.0 ** * 2.50 * *** *** *** 2.5 2.00 2.0 G Shoot apex H 1.5 1.50 WT prr79 prr59 prr57 prr5 100 CCA1 S T 80 TOC1 0.6 *** Silique length (mm) 20 *** 0.5 *** *** Seeds weight *** *** 60 0.4 40 16 *** *** 0.3 12 20 0.2 0 8 0.1 WT prr79 prr59 prr57 toc1-2 prr5 WT prr79 prr57 prr59 toc1-2 prr5 3 7 11 15 19 23 0 8 16 24 32 40 48 TIME (h) Time (h) Relative gene expression J A D G Relative gene expression 0.05 0.10 0.15 0.20 0.25 0 K E B H TA 3 WT PR 6 R 7 PR R 7 #2 12 cca1-11lhy-RNAi ELF4 Time (h) PR R 9 18 PR R 9 #2 24 0 2 4 6 I L F C TA 3 LUX PR ELF4 R 7 PR R 7 #2 PR R9 PR R 9 #2 A PRR5 seedlings B TOC1 seedlings C PRR9 seedlings Relative gene expression Relative gene expression Relative gene expression 0.4 0.6 WT WT 0.4 WT rve4,6,8 rve4,6,8 rve4,6,8 0.3 0.4 0.3 0.2 0.2 0.2 0.1 0.1 0.0 0.0 0.0 0 6 12 18 24 0 6 12 18 24 0 6 12 18 24 Time (h) Time (h) Time (h) D PRR5 pistils E TOC1 pistils F PRR9 pistils Relative gene expression Relative gene expression Relative gene expression 0.6 0.8 0.15 WT WT WT rve4,6,8 rve4,6,8 rve4,6,8 0.6 0.4 0.10 0.4 0.2 0.05 0.2 0.0 0.00 0 6 12 18 24 0 6 12 18 24 0 6 12 18 24 Time (h) Time (h) Time (h) G rve4,6,8 TOC1 Seedling vs Pistil H rve4,6,8 PRR9 Seedling vs Pistil I WT RVE4 Seedling vs Pistil Relative gene expression 0.6 Relative gene expression 1.0 Seedling Seedling Seedling 0.4 1.5 Pistil Pistil Pistil 0.8 0.4 0.3 1.0 0.6 0.2 0.4 0.2 0.5 0.1 0.2 0.0 0.0 0.0 0.0 0 6 12 18 24 0 6 12 18 24 0 6 12 18 24 Time (h) Time (h) Time (h) Relative gene expression Relative gene expression Relative gene expression 0.0 0.2 0.4 0.6 0 E C A G CCA1 6 3 12 TOC1 Time (h) WT PRR9 CCA1-ox 18 2 CCA1-ox prr79 24 Relative gene expression Relative gene expression Relative gene expression 7 0.1 0.2 0.3 PRR7 1 0 F D B 5 WT 4 6 CCA1-ox CCA1-ox prr79 12 TOC1 ELF4 Time (h) 18 EC 24 6