b9a95942cf2be02e9466cfe6cfd977da FroPlaSci_a2022v13p833113.iENG.pdf a44304d35f2a6de56230a226657b9d8e86f94aa1 FroPlaSci_a2022v13p833113.iENG.pdf dc4d443c392d9ed48de7e602095b7f046428a8e4d5cfbb2a0c45c0ea818eb8a3 FroPlaSci_a2022v13p833113.iENG.pdf Title: The Lack of Alternative Oxidase 1a Restricts in vivo Respiratory Activity and Stress-Related Metabolism for Leaf Osmoprotection and Redox Balancing Under Sudden Acute Water and Salt Stress in Arabidopsis thaliana Subject: In plants salt and water stress result in an induction of respiration and accumulation of stress-related metabolites (SRMs) with osmoregulation and osmoprotection functions that benefit photosynthesis. Keywords: water stress, salinity, alternative oxidase, oxygen-isotope fractionation, primary metabolism, photosynthesis, Arabidopsis thaliana Author: Néstor F. Del-Saz and Igor Florez-Sarasa Creator: LaTeX with hyperref package Producer: pdfTeX-1.40.15 CreationDate: Wed May 11 10:54:23 2022 CEST ModDate: Wed May 11 15:15:36 2022 CEST Tagged: no UserProperties: no Suspects: no Form: none JavaScript: no Pages: 15 Encrypted: no Page size: 595.276 x 779.528 pts Page rot: 0 File size: 8400857 bytes Optimized: no PDF version: 1.5 name type encoding emb sub uni object ID ------------------------------------ ----------------- ---------------- --- --- --- --------- RPYKOS+MinionPro-Regular Type 1 Custom yes yes no 26 0 WOARFT+HelveticaNeueLTStd-Bd Type 1 Custom yes yes no 27 0 SYPLRJ+HelveticaNeueLTStd-Lt Type 1 Custom yes yes no 28 0 KMRRIF+HelveticaNeueLTStd-BdIt Type 1 Custom yes yes no 33 0 TQRPYG+HelveticaNeueLTStd-LtIt Type 1 Custom yes yes no 34 0 BUQHYG+HelveticaNeueLTStd-MdIt Type 1 Custom yes yes no 35 0 BHDJHE+MinionPro-It Type 1 Custom yes yes no 172 0 FGSJEF+MTGU Type 1 Builtin yes yes no 253 0 IGLKJW+MTSYN Type 1 Builtin yes yes no 254 0 BEQENP+RBLMI Type 1 Builtin yes yes no 258 0 LNNFXO+wasy10 Type 1 Builtin yes yes no 259 0 TCDUEB+MinionPro-Bold Type 1 Custom yes yes no 269 0 Jhove (Rel. 1.22.1, 2019-04-17) Date: 2023-05-06 02:26:29 CEST RepresentationInformation: FroPlaSci_a2022v13p833113.iENG.pdf ReportingModule: PDF-hul, Rel. 1.12.1 (2019-04-17) LastModified: 2023-05-05 10:19:36 CEST Size: 8400857 Format: PDF Version: 1.5 Status: Well-Formed and valid SignatureMatches: PDF-hul MIMEtype: application/pdf PDFMetadata: Objects: 1108 FreeObjects: 12 IncrementalUpdates: 2 DocumentCatalog: PageLayout: SinglePage PageMode: UseOutlines Outlines: Item: Title: The Lack of Alternative Oxidase 1a Restricts in vivo Respiratory Activity and Stress-Related Metabolism for Leaf Osmoprotection and Redox Balancing Under Sudden Acute Water and Salt Stress in Arabidopsis thaliana Children: Item: Title: Introduction Item: Title: Materials and Methods Children: Item: Title: Plant Material and Growth Conditions Item: Title: Respiration and Oxygen-Isotope Fractionation Measurements Item: Title: Leaf Water Status Item: Title: Photosynthetic Measurements Item: Title: Metabolite Profiling Item: Title: Western Blot Analysis Item: Title: Gene Expression Analysis Item: Title: Statistical Analyses Item: Title: Results Children: Item: Title: The Alternative Oxidase Capacity and Expression in aox1a and Wild-Type Plants Under Control and Stress Conditions Item: Title: Leaf Phenotype and Water Status in aox1a and Wild-Type Plants Under Control and Stress Conditions Item: Title: Respiration and Electron Partitioning to the Alternative Oxidase Pathway in aox1a and Wild-Type Plants Under Control and Stress Conditions Item: Title: Photosynthetic Parameters in aox1a and Wild-Type Plants Under Control and Stress Conditions Item: Title: Metabolite Profiling in aox1a and Wild-Type Plants Under Control and Stress Conditions Item: Title: Gene Expression of Other Alternative Respiratory Components in aox1a and Wild-Type Plants Under Control and Stress Conditions Item: Title: Discussion Children: Item: Title: The Lack of AOX1a Does Not Impair Alternative Oxidase Pathway in vivo Activity Under Control Conditions but Restricted Its Response to Stress Item: Title: The Lack in vivo Alternative Oxidase Pathway Response Under Stress Altered the Accumulation of Stress-Related Metabolities Involved in Leaf Osmoregulation and Redox Balance Item: Title: Conclusion Item: Title: Data Availability Statement Item: Title: Author Contributions Item: Title: Funding Item: Title: Acknowledgments Item: Title: Supplementary Material Item: Title: References Info: Title: The Lack of Alternative Oxidase 1a Restricts in vivo Respiratory Activity and Stress-Related Metabolism for Leaf Osmoprotection and Redox Balancing Under Sudden Acute Water and Salt Stress in Arabidopsis thaliana Author: Néstor F. Del-Saz and Igor Florez-Sarasa Subject: In plants salt and water stress result in an induction of respiration and accumulation of stress-related metabolites (SRMs) with osmoregulation and osmoprotection functions that benefit photosynthesis. 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Néstor F. 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