20c4c28e94afbdcc321b5f3623da2d18 plants_a2022m11v11n22p3120.pdf 5808691dde72260b3ff11771cf22a23ce5a0e450 plants_a2022m11v11n22p3120.pdf d426036fe8b8dff929a8ad4322d03142e41de883e2f435028033e142959fbc56 plants_a2022m11v11n22p3120.pdf Title: Fruit Morphology and Ripening-Related QTLs in a Newly Developed Introgression Line Collection of the Elite Varieties ‘Védrantais’ and ‘Piel de Sapo’ Subject: Melon is an economically important crop with widely diverse fruit morphology and ripening characteristics. Its diploid sequenced genome and multiple genomic tools make this species suitable to study the genetic architecture of fruit traits. With the development of this introgression line population of the elite varieties ‘Piel de Sapo’ and ‘Védrantais’, we present a powerful tool to study fruit morphology and ripening traits that can also facilitate characterization or pyramidation of QTLs in inodorous melon types. The population consists of 36 lines covering almost 98% of the melon genome, with an average of three introgressions per chromosome and segregating for multiple fruit traits: morphology, ripening and quality. High variability in fruit morphology was found within the population, with 24 QTLs affecting six different traits, confirming previously reported QTLs and two newly detected QTLs, FLQW5.1 and FWQW7.1. We detected 20 QTLs affecting fruit ripening traits, six of them reported for the first time, two affecting the timing of yellowing of the rind (EYELLQW1.1 and EYELLQW8.1) and four at the end of chromosome 8 affecting aroma, abscission and harvest date (EAROQW8.3, EALFQW8.3, ABSQW8.3 and HARQW8.3). We also confirmed the location of several QTLs, such as fruit-quality-related QTLs affecting rind and flesh appearance and flesh firmness. Keywords: introgression lines; Cucumis melo; fruit quality; climacteric ripening Author: Miguel Santo Domingo, Carlos Mayobre, Lara Pereira, Jason Argyris, Laura Valverde, Ana Montserrat Martín-Hernández, Jordi Garcia-Mas and Marta Pujol Creator: LaTeX with hyperref Producer: pdfTeX-1.40.21 CreationDate: Mon Nov 21 08:47:43 2022 CET ModDate: Mon Nov 21 08:55:12 2022 CET Tagged: no UserProperties: no Suspects: no Form: none JavaScript: no Pages: 18 Encrypted: no Page size: 595.276 x 841.89 pts (A4) Page rot: 0 File size: 5468606 bytes Optimized: no PDF version: 1.7 name type encoding emb sub uni object ID ------------------------------------ ----------------- ---------------- --- --- --- --------- PGRUXE+URWPalladioL-Bold Type 1 Custom yes yes no 10 0 NFNOVH+URWPalladioL-Roma Type 1 Custom yes yes no 15 0 QPBQZP+URWPalladioL-Ital Type 1 Custom yes yes no 19 0 ZWPWZO+VnURWPalladioL Type 1 Custom yes yes no 23 0 IOJCNK+VnURWPalladioL-Bold Type 1 Custom yes yes no 28 0 VTLKDP+TimesNewRomanPS-BoldItalicMT Type 1C WinAnsi yes yes no 47 0 VIWIOF+CMSY10 Type 1 Builtin yes yes no 77 0 IGJDOL+PalatinoLinotype-Bold CID TrueType Identity-H yes yes yes 87 0 IGJDPL+PalatinoLinotype-Roman CID TrueType Identity-H yes yes yes 93 0 IGJEBM+PalatinoLinotype-Italic CID TrueType Identity-H yes yes yes 99 0 IGJEBN+PalatinoLinotype-Italic TrueType WinAnsi yes yes no 105 0 IGJEBO+PalatinoLinotype-Bold TrueType WinAnsi yes yes no 108 0 IGJEBP+PalatinoLinotype-Roman TrueType WinAnsi yes yes no 111 0 JLZORU+EURM10 Type 1 Builtin yes yes no 118 0 IGJDOL+PalatinoLinotype-Bold CID TrueType Identity-H yes yes yes 127 0 IGJDPL+PalatinoLinotype-Roman CID TrueType Identity-H yes yes yes 133 0 IGJEBM+PalatinoLinotype-Italic CID TrueType Identity-H yes yes yes 139 0 IGJEBN+PalatinoLinotype-Italic TrueType WinAnsi yes yes no 145 0 IGJEBO+PalatinoLinotype-Bold TrueType WinAnsi yes yes no 148 0 IGJEBP+PalatinoLinotype-Roman TrueType WinAnsi yes yes no 151 0 IGJDOL+PalatinoLinotype-Bold CID TrueType Identity-H yes yes yes 161 0 IGJDPL+PalatinoLinotype-Roman CID TrueType Identity-H yes yes yes 167 0 IGJEBM+PalatinoLinotype-Italic CID TrueType Identity-H yes yes yes 173 0 IGJEBN+PalatinoLinotype-Italic TrueType WinAnsi yes yes no 179 0 IGJEBO+PalatinoLinotype-Bold TrueType WinAnsi yes yes no 182 0 IGJEBP+PalatinoLinotype-Roman TrueType WinAnsi yes yes no 185 0 IGJDOL+PalatinoLinotype-Bold CID TrueType Identity-H yes yes yes 198 0 IGJDPL+PalatinoLinotype-Roman CID TrueType Identity-H yes yes yes 204 0 IGJEBM+PalatinoLinotype-Italic CID TrueType Identity-H yes yes yes 210 0 IGJEBN+PalatinoLinotype-Italic TrueType WinAnsi yes yes no 216 0 IGJEBO+PalatinoLinotype-Bold TrueType WinAnsi yes yes no 219 0 IGJEBP+PalatinoLinotype-Roman TrueType WinAnsi yes yes no 222 0 WBVKDJ+URWPalladioL-BoldItal Type 1 Custom yes yes no 229 0 IGJDOL+PalatinoLinotype-Bold CID TrueType Identity-H yes yes yes 242 0 IGJDPL+PalatinoLinotype-Roman CID TrueType Identity-H yes yes yes 248 0 IGJEBM+PalatinoLinotype-Italic CID TrueType Identity-H yes yes yes 254 0 IGJEBN+PalatinoLinotype-Italic TrueType WinAnsi yes yes no 260 0 IGJEBO+PalatinoLinotype-Bold TrueType WinAnsi yes yes no 263 0 IGJEBP+PalatinoLinotype-Roman TrueType WinAnsi yes yes no 266 0 IGJDOL+PalatinoLinotype-Bold CID TrueType Identity-H yes yes yes 276 0 IGJDPL+PalatinoLinotype-Roman CID TrueType Identity-H yes yes yes 282 0 IGJEBM+PalatinoLinotype-Italic CID TrueType Identity-H yes yes yes 288 0 IGJEBN+PalatinoLinotype-Italic TrueType WinAnsi yes yes no 294 0 IGJEBO+PalatinoLinotype-Bold TrueType WinAnsi yes yes no 297 0 IGJEBP+PalatinoLinotype-Roman TrueType WinAnsi yes yes no 300 0 Jhove (Rel. 1.22.1, 2019-04-17) Date: 2023-05-05 02:30:54 CEST RepresentationInformation: plants_a2022m11v11n22p3120.pdf ReportingModule: PDF-hul, Rel. 1.12.1 (2019-04-17) LastModified: 2023-05-04 10:37:48 CEST Size: 5468606 Format: PDF Version: 1.7 Status: Well-Formed and valid SignatureMatches: PDF-hul MIMEtype: application/pdf PDFMetadata: Objects: 606 FreeObjects: 1 IncrementalUpdates: 0 DocumentCatalog: PageLayout: SinglePage PageMode: UseNone Outlines: Item: Title: Introduction Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@44c8afef Item: Title: Results and Discussion Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@7b69c6ba Children: Item: Title: Phenotyping of the Parental Lines Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@46daef40 Item: Title: Development of the IL Collection Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@12f41634 Item: Title: Phenotyping of the IL Population and QTL Mapping Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@13c27452 Children: Item: Title: Fruit Quality Traits Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@262b2c86 Item: Title: Fruit Morphology Traits Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@371a67ec Item: Title: Ripening Traits Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@5ed828d Item: Title: Materials and Methods Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@50d0686 Children: Item: Title: Plant Material and Breeding Scheme Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@7a3d45bd Item: Title: In Vitro Plant Culture Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@1e7c7811 Item: Title: DNA Extraction and Genotyping Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@77ec78b9 Item: Title: Experimental Design and Phenotyping Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@1a3869f4 Item: Title: QTL Mapping and Data Analysis Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@a38d7a3 Item: Title: Conclusions Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@77f99a05 Item: Title: References Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@63440df3 Info: Title: Fruit Morphology and Ripening-Related QTLs in a Newly Developed Introgression Line Collection of the Elite Varieties ‘Védrantais’ and ‘Piel de Sapo’ Author: Miguel Santo Domingo, Carlos Mayobre, Lara Pereira, Jason Argyris, Laura Valverde, Ana Montserrat Martín-Hernández, Jordi Garcia-Mas and Marta Pujol Subject: Melon is an economically important crop with widely diverse fruit morphology and ripening characteristics. Its diploid sequenced genome and multiple genomic tools make this species suitable to study the genetic architecture of fruit traits. With the development of this introgression line population of the elite varieties ‘Piel de Sapo’ and ‘Védrantais’, we present a powerful tool to study fruit morphology and ripening traits that can also facilitate characterization or pyramidation of QTLs in inodorous melon types. The population consists of 36 lines covering almost 98% of the melon genome, with an average of three introgressions per chromosome and segregating for multiple fruit traits: morphology, ripening and quality. High variability in fruit morphology was found within the population, with 24 QTLs affecting six different traits, confirming previously reported QTLs and two newly detected QTLs, FLQW5.1 and FWQW7.1. We detected 20 QTLs affecting fruit ripening traits, six of them reported for the first time, two affecting the timing of yellowing of the rind (EYELLQW1.1 and EYELLQW8.1) and four at the end of chromosome 8 affecting aroma, abscission and harvest date (EAROQW8.3, EALFQW8.3, ABSQW8.3 and HARQW8.3). We also confirmed the location of several QTLs, such as fruit-quality-related QTLs affecting rind and flesh appearance and flesh firmness. Keywords: introgression lines; Cucumis melo; fruit quality; climacteric ripening Creator: LaTeX with hyperref Producer: pdfTeX-1.40.21 CreationDate: Mon Nov 21 08:47:43 CET 2022 ModDate: Mon Nov 21 08:55:12 CET 2022 ID: 0xd0e90bc1d73cc6eb92c267f41ddb452a, 0xd0e90bc1d73cc6eb92c267f41ddb452a Filters: FilterPipeline: FlateDecode Fonts: Type1: Font: BaseFont: QPBQZP+URWPalladioL-Ital FontSubset: true FirstChar: 40 LastChar: 122 FontDescriptor: FontName: QPBQZP+URWPalladioL-Ital Flags: Symbolic FontBBox: -170, -305, 1010, 941 FontFile: true EncodingDictionary: Differences: true Font: BaseFont: WBVKDJ+URWPalladioL-BoldItal FontSubset: true FirstChar: 112 LastChar: 112 FontDescriptor: FontName: WBVKDJ+URWPalladioL-BoldItal Flags: Symbolic FontBBox: -170, -300, 1073, 935 FontFile: true EncodingDictionary: Differences: true Font: BaseFont: JLZORU+EURM10 FontSubset: true FirstChar: 31 LastChar: 31 FontDescriptor: FontName: JLZORU+EURM10 Flags: Symbolic FontBBox: -32, -243, 1060, 720 FontFile: true Font: BaseFont: ZWPWZO+VnURWPalladioL FontSubset: true FirstChar: 128 LastChar: 243 FontDescriptor: FontName: ZWPWZO+VnURWPalladioL Flags: Symbolic FontBBox: -166, -283, 1021, 943 FontFile: true EncodingDictionary: Differences: true Font: BaseFont: PGRUXE+URWPalladioL-Bold FontSubset: true FirstChar: 3 LastChar: 134 FontDescriptor: FontName: PGRUXE+URWPalladioL-Bold Flags: Symbolic FontBBox: -152, -301, 1000, 935 FontFile: true EncodingDictionary: Differences: true Font: BaseFont: IOJCNK+VnURWPalladioL-Bold FontSubset: true FirstChar: 161 LastChar: 189 FontDescriptor: FontName: IOJCNK+VnURWPalladioL-Bold Flags: Symbolic FontBBox: -152, -301, 1000, 935 FontFile: true EncodingDictionary: Differences: true Font: BaseFont: VIWIOF+CMSY10 FontSubset: true FirstChar: 0 LastChar: 48 FontDescriptor: FontName: VIWIOF+CMSY10 Flags: Symbolic FontBBox: -29, -960, 1116, 775 FontFile: true Font: BaseFont: NFNOVH+URWPalladioL-Roma FontSubset: true FirstChar: 2 LastChar: 241 FontDescriptor: FontName: NFNOVH+URWPalladioL-Roma Flags: Symbolic FontBBox: -166, -283, 1021, 943 FontFile: true EncodingDictionary: Differences: true Pages: Page: Sequence: 1 Annotations: Annotation: Subtype: Link Rect: 34, 323, 152, 332 Annotation: Subtype: Link Rect: 34, 311, 83, 320 Annotation: Subtype: Link Rect: 34, 164, 96, 186 Annotation: Subtype: Link Rect: 126, 90, 154, 99 Annotation: Subtype: Link Rect: 34, 78, 147, 87 Annotation: Subtype: Link Rect: 34, 756, 142, 792 Annotation: Subtype: Link Rect: 515, 756, 560, 792 Annotation: Subtype: Link Rect: 155, 642, 166, 655 Annotation: Subtype: Link Rect: 256, 642, 267, 655 Annotation: Subtype: Link Rect: 331, 642, 342, 655 Annotation: Subtype: Link Rect: 310, 629, 321, 643 Annotation: Subtype: Link Rect: 415, 629, 426, 643 Annotation: Subtype: Link Rect: 416, 197, 423, 206 ActionDest: 317 Annotation: Subtype: Link Rect: 426, 197, 433, 206 ActionDest: 317 Annotation: Subtype: Link Rect: 315, 172, 322, 181 ActionDest: 317 Annotation: Subtype: Link Rect: 325, 172, 332, 181 ActionDest: 317 Annotation: Subtype: Link Rect: 431, 172, 438, 181 ActionDest: 317 Annotation: Subtype: Link Rect: 439, 172, 445, 181 ActionDest: 317 Annotation: Subtype: Link Rect: 477, 159, 484, 169 ActionDest: 317 Annotation: Subtype: Link Rect: 208, 147, 220, 156 ActionDest: 317 Annotation: Subtype: Link Rect: 544, 147, 556, 156 ActionDest: 317 Annotation: Subtype: Link Rect: 345, 135, 357, 143 ActionDest: 317 Annotation: Subtype: Link Rect: 532, 109, 544, 118 ActionDest: 317 Annotation: Subtype: Link Rect: 545, 109, 557, 118 ActionDest: 317 Annotation: Subtype: Link Rect: 402, 97, 414, 106 ActionDest: 317 Annotation: Subtype: Link Rect: 414, 97, 426, 106 ActionDest: 317 Annotation: Subtype: Link Rect: 109, 17, 255, 28 Annotation: Subtype: Link Rect: 415, 17, 560, 28 Annotation: Subtype: Link Rect: 34, 444, 87, 463 Page: Sequence: 2 Annotations: Annotation: Subtype: Link Rect: 389, 735, 401, 744 ActionDest: 317 Annotation: Subtype: Link Rect: 517, 736, 529, 744 ActionDest: 317 Annotation: Subtype: Link Rect: 232, 723, 243, 732 ActionDest: 317 Annotation: Subtype: Link Rect: 378, 723, 390, 732 ActionDest: 317 Annotation: Subtype: Link Rect: 390, 723, 402, 732 ActionDest: 317 Annotation: Subtype: Link Rect: 326, 698, 338, 707 ActionDest: 317 Annotation: Subtype: Link Rect: 544, 698, 556, 707 ActionDest: 317 Annotation: Subtype: Link Rect: 182, 610, 194, 619 ActionDest: 317 Annotation: Subtype: Link Rect: 197, 610, 209, 619 ActionDest: 320 Annotation: Subtype: Link Rect: 316, 610, 328, 619 ActionDest: 320 Annotation: Subtype: Link Rect: 331, 610, 343, 619 ActionDest: 320 Annotation: Subtype: Link Rect: 221, 585, 233, 594 ActionDest: 320 Annotation: Subtype: Link Rect: 234, 585, 246, 594 ActionDest: 320 Annotation: Subtype: Link Rect: 246, 585, 258, 594 ActionDest: 320 Annotation: Subtype: Link Rect: 489, 572, 501, 581 ActionDest: 320 Annotation: Subtype: Link Rect: 502, 572, 514, 581 ActionDest: 320 Annotation: Subtype: Link Rect: 318, 547, 330, 556 ActionDest: 320 Annotation: Subtype: Link Rect: 331, 547, 343, 556 ActionDest: 320 Annotation: Subtype: Link Rect: 351, 522, 363, 531 ActionDest: 320 Annotation: Subtype: Link Rect: 544, 355, 551, 367 ActionDest: 81 Annotation: Subtype: Link Rect: 410, 317, 417, 329 ActionDest: 81 Annotation: Subtype: Link Rect: 507, 295, 518, 304 ActionDest: 320 Annotation: Subtype: Link Rect: 519, 295, 531, 304 ActionDest: 320 Annotation: Subtype: Link Rect: 531, 295, 543, 304 ActionDest: 320 Annotation: Subtype: Link Rect: 544, 295, 556, 304 ActionDest: 320 Annotation: Subtype: Link Rect: 322, 270, 334, 279 ActionDest: 320 Annotation: Subtype: Link Rect: 334, 270, 346, 279 ActionDest: 320 Annotation: Subtype: Link Rect: 347, 270, 359, 279 ActionDest: 320 Annotation: Subtype: Link Rect: 293, 179, 301, 191 ActionDest: 81 Annotation: Subtype: Link Rect: 318, 157, 330, 166 ActionDest: 320 Annotation: Subtype: Link Rect: 331, 157, 343, 166 ActionDest: 320 Annotation: Subtype: Link Rect: 281, 116, 288, 128 ActionDest: 81 Annotation: Subtype: Link Rect: 353, 69, 365, 78 ActionDest: 320 Page: Sequence: 3 Annotations: Annotation: Subtype: Link Rect: 310, 720, 317, 732 ActionDest: 81 Annotation: Subtype: Link Rect: 408, 710, 420, 719 ActionDest: 320 Annotation: Subtype: Link Rect: 420, 710, 432, 719 ActionDest: 320 Annotation: Subtype: Link Rect: 433, 710, 445, 719 ActionDest: 320 Annotation: Subtype: Link Rect: 215, 198, 227, 207 ActionDest: 320 Annotation: Subtype: Link Rect: 415, 183, 422, 195 ActionDest: 115 Page: Sequence: 4 Annotations: Annotation: Subtype: Link Rect: 482, 160, 494, 169 ActionDest: 320 Annotation: Subtype: Link Rect: 410, 107, 417, 119 ActionDest: 155 Annotation: Subtype: Link Rect: 469, 107, 476, 119 ActionDest: 155 Annotation: Subtype: Link Rect: 503, 72, 515, 81 ActionDest: 317 Annotation: Subtype: Link Rect: 516, 72, 528, 81 ActionDest: 320 Annotation: Subtype: Link Rect: 528, 72, 540, 81 ActionDest: 320 Page: Sequence: 5 Annotations: Annotation: Subtype: Link Rect: 396, 518, 406, 526 ActionDest: 317 Page: Sequence: 6 Annotations: Annotation: Subtype: Link Rect: 268, 682, 279, 691 ActionDest: 320 Annotation: Subtype: Link Rect: 199, 566, 206, 578 ActionDest: 192 Annotation: Subtype: Link Rect: 519, 554, 526, 566 ActionDest: 226 Annotation: Subtype: Link Rect: 364, 529, 371, 541 ActionDest: 192 Annotation: Subtype: Link Rect: 296, 506, 308, 515 ActionDest: 317 Annotation: Subtype: Link Rect: 309, 506, 321, 515 ActionDest: 320 Annotation: Subtype: Link Rect: 321, 506, 333, 515 ActionDest: 320 Annotation: Subtype: Link Rect: 244, 481, 256, 490 ActionDest: 320 Annotation: Subtype: Link Rect: 200, 441, 207, 453 ActionDest: 192 Annotation: Subtype: Link Rect: 207, 431, 219, 440 ActionDest: 320 Annotation: Subtype: Link Rect: 283, 418, 295, 427 ActionDest: 317 Annotation: Subtype: Link Rect: 295, 418, 307, 427 ActionDest: 320 Annotation: Subtype: Link Rect: 520, 381, 532, 390 ActionDest: 320 Annotation: Subtype: Link Rect: 483, 365, 490, 377 ActionDest: 192 Annotation: Subtype: Link Rect: 390, 356, 402, 365 ActionDest: 320 Annotation: Subtype: Link Rect: 380, 315, 387, 327 ActionDest: 192 Annotation: Subtype: Link Rect: 297, 305, 308, 314 ActionDest: 317 Annotation: Subtype: Link Rect: 309, 305, 321, 314 ActionDest: 320 Annotation: Subtype: Link Rect: 355, 293, 367, 302 ActionDest: 320 Annotation: Subtype: Link Rect: 199, 265, 206, 277 ActionDest: 155 Annotation: Subtype: Link Rect: 211, 230, 223, 239 ActionDest: 320 Annotation: Subtype: Link Rect: 223, 230, 235, 239 ActionDest: 320 Annotation: Subtype: Link Rect: 286, 217, 298, 227 ActionDest: 320 Annotation: Subtype: Link Rect: 410, 218, 422, 227 ActionDest: 320 Annotation: Subtype: Link Rect: 350, 190, 357, 202 ActionDest: 192 Annotation: Subtype: Link Rect: 388, 167, 400, 176 ActionDest: 320 Annotation: Subtype: Link Rect: 410, 142, 422, 151 ActionDest: 320 Annotation: Subtype: Link Rect: 425, 142, 437, 151 ActionDest: 320 Page: Sequence: 7 Page: Sequence: 8 Page: Sequence: 9 Annotations: Annotation: Subtype: Link Rect: 200, 708, 207, 720 ActionDest: 192 Annotation: Subtype: Link Rect: 195, 696, 202, 707 ActionDest: 226 Annotation: Subtype: Link Rect: 316, 597, 328, 606 ActionDest: 320 Annotation: Subtype: Link Rect: 467, 570, 474, 582 ActionDest: 192 Annotation: Subtype: Link Rect: 426, 560, 438, 569 ActionDest: 320 Annotation: Subtype: Link Rect: 510, 494, 517, 506 ActionDest: 81 Annotation: Subtype: Link Rect: 541, 456, 548, 468 ActionDest: 236 Annotation: Subtype: Link Rect: 224, 431, 231, 443 ActionDest: 236 Annotation: Subtype: Link Rect: 259, 431, 266, 443 ActionDest: 226 Annotation: Subtype: Link Rect: 230, 396, 242, 405 ActionDest: 320 Annotation: Subtype: Link Rect: 531, 358, 543, 368 ActionDest: 317 Annotation: Subtype: Link Rect: 543, 358, 555, 368 ActionDest: 323 Annotation: Subtype: Link Rect: 308, 281, 315, 293 ActionDest: 236 Annotation: Subtype: Link Rect: 346, 281, 353, 293 ActionDest: 226 Annotation: Subtype: Link Rect: 354, 271, 366, 280 ActionDest: 323 Annotation: Subtype: Link Rect: 366, 271, 378, 280 ActionDest: 323 Annotation: Subtype: Link Rect: 237, 255, 244, 267 ActionDest: 236 Annotation: Subtype: Link Rect: 300, 183, 312, 192 ActionDest: 320 Annotation: Subtype: Link Rect: 197, 130, 204, 142 ActionDest: 236 Annotation: Subtype: Link Rect: 473, 107, 485, 117 ActionDest: 323 Annotation: Subtype: Link Rect: 320, 82, 331, 91 ActionDest: 317 Annotation: Subtype: Link Rect: 311, 67, 318, 79 ActionDest: 236 Page: Sequence: 10 Annotations: Annotation: Subtype: Link Rect: 340, 723, 352, 732 ActionDest: 323 Annotation: Subtype: Link Rect: 347, 710, 359, 719 ActionDest: 320 Annotation: Subtype: Link Rect: 460, 286, 467, 298 ActionDest: 236 Annotation: Subtype: Link Rect: 334, 261, 341, 273 ActionDest: 226 Annotation: Subtype: Link Rect: 320, 223, 327, 235 ActionDest: 236 Annotation: Subtype: Link Rect: 513, 213, 525, 222 ActionDest: 317 Annotation: Subtype: Link Rect: 525, 213, 537, 222 ActionDest: 317 Annotation: Subtype: Link Rect: 246, 200, 258, 209 ActionDest: 323 Annotation: Subtype: Link Rect: 203, 150, 215, 159 ActionDest: 317 Annotation: Subtype: Link Rect: 328, 75, 340, 84 ActionDest: 317 Annotation: Subtype: Link Rect: 384, 75, 396, 84 ActionDest: 317 Annotation: Subtype: Link Rect: 493, 75, 505, 84 ActionDest: 323 Page: Sequence: 11 Annotations: Annotation: Subtype: Link Rect: 437, 735, 449, 744 ActionDest: 323 Annotation: Subtype: Link Rect: 418, 557, 425, 569 ActionDest: 81 Annotation: Subtype: Link Rect: 239, 494, 246, 506 ActionDest: 270 Annotation: Subtype: Link Rect: 371, 472, 383, 481 ActionDest: 320 Annotation: Subtype: Link Rect: 270, 446, 282, 455 ActionDest: 323 Annotation: Subtype: Link Rect: 283, 446, 295, 455 ActionDest: 323 Annotation: Subtype: Link Rect: 376, 434, 388, 443 ActionDest: 320 Annotation: Subtype: Link Rect: 389, 434, 400, 443 ActionDest: 323 Page: Sequence: 12 Annotations: Annotation: Subtype: Link Rect: 194, 683, 202, 695 ActionDest: 226 Annotation: Subtype: Link Rect: 296, 683, 303, 695 ActionDest: 270 Annotation: Subtype: Link Rect: 502, 660, 514, 669 ActionDest: 320 Annotation: Subtype: Link Rect: 514, 660, 526, 669 ActionDest: 320 Annotation: Subtype: Link Rect: 544, 635, 556, 644 ActionDest: 323 Annotation: Subtype: Link Rect: 413, 597, 425, 606 ActionDest: 320 Annotation: Subtype: Link Rect: 428, 560, 440, 569 ActionDest: 320 Annotation: Subtype: Link Rect: 485, 535, 497, 544 ActionDest: 320 Annotation: Subtype: Link Rect: 322, 494, 329, 506 ActionDest: 155 Annotation: Subtype: Link Rect: 393, 434, 405, 443 ActionDest: 320 Annotation: Subtype: Link Rect: 406, 434, 418, 443 ActionDest: 320 Annotation: Subtype: Link Rect: 418, 434, 430, 443 ActionDest: 323 Annotation: Subtype: Link Rect: 447, 359, 459, 368 ActionDest: 320 Annotation: Subtype: Link Rect: 552, 356, 559, 368 ActionDest: 192 Annotation: Subtype: Link Rect: 355, 331, 362, 343 ActionDest: 270 Annotation: Subtype: Link Rect: 199, 306, 206, 318 ActionDest: 270 Annotation: Subtype: Link Rect: 307, 293, 314, 305 ActionDest: 226 Annotation: Subtype: Link Rect: 349, 293, 356, 305 ActionDest: 270 Annotation: Subtype: Link Rect: 193, 268, 200, 280 ActionDest: 226 Page: Sequence: 13 Annotations: Annotation: Subtype: Link Rect: 509, 670, 516, 682 ActionDest: 115 Annotation: Subtype: Link Rect: 464, 471, 476, 480 ActionDest: 323 Annotation: Subtype: Link Rect: 209, 459, 221, 468 ActionDest: 317 Annotation: Subtype: Link Rect: 203, 446, 215, 455 ActionDest: 323 Annotation: Subtype: Link Rect: 544, 434, 556, 443 ActionDest: 320 Annotation: Subtype: Link Rect: 309, 409, 321, 417 ActionDest: 320 Annotation: Subtype: Link Rect: 202, 396, 214, 405 ActionDest: 323 Annotation: Subtype: Link Rect: 217, 371, 229, 380 ActionDest: 320 Annotation: Subtype: Link Rect: 490, 220, 502, 229 ActionDest: 317 Annotation: Subtype: Link Rect: 314, 117, 321, 129 ActionDest: 311 Page: Sequence: 14 Annotations: Annotation: Subtype: Link Rect: 478, 364, 485, 376 ActionDest: 311 Annotation: Subtype: Link Rect: 299, 339, 306, 351 ActionDest: 311 Annotation: Subtype: Link Rect: 285, 278, 297, 288 ActionDest: 323 Page: Sequence: 15 Annotations: Annotation: Subtype: Link Rect: 402, 657, 414, 666 ActionDest: 323 Annotation: Subtype: Link Rect: 392, 519, 404, 528 ActionDest: 320 Annotation: Subtype: Link Rect: 536, 379, 560, 390 Annotation: Subtype: Link Rect: 165, 367, 384, 378 Page: Sequence: 16 Annotations: Annotation: Subtype: Link Rect: 475, 707, 513, 718 Annotation: Subtype: Link Rect: 519, 707, 555, 718 Annotation: Subtype: Link Rect: 489, 684, 526, 695 Annotation: Subtype: Link Rect: 340, 650, 377, 661 Annotation: Subtype: Link Rect: 342, 627, 379, 638 Annotation: Subtype: Link Rect: 95, 593, 132, 603 Annotation: Subtype: Link Rect: 462, 569, 499, 580 Annotation: Subtype: Link Rect: 506, 569, 542, 580 Annotation: Subtype: Link Rect: 95, 547, 133, 557 Annotation: Subtype: Link Rect: 257, 523, 295, 534 Annotation: Subtype: Link Rect: 489, 500, 527, 511 Annotation: Subtype: Link Rect: 360, 477, 397, 488 Annotation: Subtype: Link Rect: 59, 443, 96, 453 Annotation: Subtype: Link Rect: 59, 409, 96, 419 Annotation: Subtype: Link Rect: 355, 385, 392, 396 Annotation: Subtype: Link Rect: 399, 385, 435, 396 Annotation: Subtype: Link Rect: 438, 362, 475, 373 Annotation: Subtype: Link Rect: 482, 362, 518, 373 Annotation: Subtype: Link Rect: 404, 339, 441, 350 Annotation: Subtype: Link Rect: 447, 339, 483, 350 Annotation: Subtype: Link Rect: 144, 305, 181, 315 Annotation: Subtype: Link Rect: 187, 305, 223, 315 Annotation: Subtype: Link Rect: 194, 281, 231, 292 Annotation: Subtype: Link Rect: 90, 248, 128, 258 Annotation: Subtype: Link Rect: 499, 224, 536, 235 Annotation: Subtype: Link Rect: 95, 190, 132, 200 Annotation: Subtype: Link Rect: 90, 156, 128, 166 Annotation: Subtype: Link Rect: 177, 121, 214, 131 Annotation: Subtype: Link Rect: 220, 121, 256, 131 Annotation: Subtype: Link Rect: 445, 97, 483, 108 Annotation: Subtype: Link Rect: 489, 97, 525, 108 Annotation: Subtype: Link Rect: 122, 64, 159, 74 Annotation: Subtype: Link Rect: 165, 64, 201, 74 Page: Sequence: 17 Annotations: Annotation: Subtype: Link Rect: 232, 722, 269, 733 Annotation: Subtype: Link Rect: 436, 687, 474, 698 Annotation: Subtype: Link Rect: 86, 653, 123, 664 Annotation: Subtype: Link Rect: 386, 629, 423, 640 Annotation: Subtype: Link Rect: 291, 595, 328, 606 Annotation: Subtype: Link Rect: 59, 561, 96, 571 Annotation: Subtype: Link Rect: 473, 537, 511, 548 Annotation: Subtype: Link Rect: 277, 503, 314, 514 Annotation: Subtype: Link Rect: 328, 480, 366, 491 Annotation: Subtype: Link Rect: 372, 480, 408, 491 Annotation: Subtype: Link Rect: 390, 457, 428, 468 Annotation: Subtype: Link Rect: 434, 457, 470, 468 Annotation: Subtype: Link Rect: 207, 423, 245, 433 Annotation: Subtype: Link Rect: 230, 388, 267, 399 Annotation: Subtype: Link Rect: 388, 353, 426, 364 Annotation: Subtype: Link Rect: 431, 330, 560, 341 Annotation: Subtype: Link Rect: 56, 319, 318, 330 Annotation: Subtype: Link Rect: 245, 297, 282, 307 Annotation: Subtype: Link Rect: 176, 250, 214, 261 Annotation: Subtype: Link Rect: 140, 216, 177, 226 Annotation: Subtype: Link Rect: 184, 216, 220, 226 Annotation: Subtype: Link Rect: 447, 192, 484, 203 Annotation: Subtype: Link Rect: 491, 192, 527, 203 Annotation: Subtype: Link Rect: 520, 169, 557, 180 Annotation: Subtype: Link Rect: 59, 159, 95, 169 Annotation: Subtype: Link Rect: 131, 124, 168, 134 Annotation: Subtype: Link Rect: 473, 101, 511, 111 Annotation: Subtype: Link Rect: 240, 66, 277, 77 Page: Sequence: 18 Annotations: Annotation: Subtype: Link Rect: 423, 722, 460, 733 Annotation: Subtype: Link Rect: 500, 699, 538, 710 Annotation: Subtype: Link Rect: 59, 665, 96, 675 Annotation: Subtype: Link Rect: 151, 630, 189, 640 Annotation: Subtype: Link Rect: 214, 607, 252, 617 Annotation: Subtype: Link Rect: 258, 607, 294, 617 Annotation: Subtype: Link Rect: 368, 583, 406, 594 Annotation: Subtype: Link Rect: 412, 583, 448, 594 Annotation: Subtype: Link Rect: 282, 560, 320, 571 Annotation: Subtype: Link Rect: 109, 527, 146, 537 Annotation: Subtype: Link Rect: 152, 527, 188, 537 Annotation: Subtype: Link Rect: 421, 491, 458, 502 Annotation: Subtype: Link Rect: 406, 457, 444, 468 Annotation: Subtype: Link Rect: 140, 423, 177, 433 Annotation: Subtype: Link Rect: 59, 389, 96, 399 Annotation: Subtype: Link Rect: 148, 365, 265, 376 Checksum: dc326ad5 Type: CRC32 Checksum: 20c4c28e94afbdcc321b5f3623da2d18 Type: MD5 Checksum: 5808691dde72260b3ff11771cf22a23ce5a0e450 Type: SHA-1