ec2237c1ac47a4e93c364659eb126817 scidat_a2022v9p90.pdf d1d11efe57d604d874cbca72ba05bb5aefe28000 scidat_a2022v9p90.pdf ff9d027c5a08f7e58e3c09e0af8b8ff6cf38e1edd1b4b305feeb4f1ca9ebbffe scidat_a2022v9p90.pdf Title: Analysis of metabolic dynamics during drought stress in Arabidopsis plants Subject: Scientific Data, doi:10.1038/s41597-022-01161-4 Keywords: Author: Fidel Lozano-Elena Creator: Springer Producer: iText® 5.3.5 ©2000-2012 1T3XT BVBA (SPRINGER SBM; licensed version) CreationDate: Tue Mar 15 10:56:45 2022 CET ModDate: Tue Mar 15 14:41:17 2022 CET Tagged: no UserProperties: no Suspects: no Form: AcroForm JavaScript: no Pages: 12 Encrypted: no Page size: 595.276 x 782.362 pts Page rot: 0 File size: 3615338 bytes Optimized: no PDF version: 1.4 name type encoding emb sub uni object ID ------------------------------------ ----------------- ---------------- --- --- --- --------- MyriadPro-It Type 1C WinAnsi yes no yes 44 0 MinionPro-It Type 1C Custom yes no yes 45 0 MinionPro-Regular Type 1C Custom yes no yes 46 0 KMYOHE+ArialUnicodeMS CID TrueType Identity-H yes yes yes 445 0 BHYKMX+Corbel-Bold2 Type 1C WinAnsi yes yes yes 47 0 HDDARK+Corbel-Bold CID TrueType Identity-H yes yes yes 170 0 MPMRGQ+Corbel-BoldItalic2 Type 1C WinAnsi yes yes yes 446 0 CQTNZU+Corbel-BoldItalic CID TrueType Identity-H yes yes yes 447 0 SNNFVA+Corbel2 Type 1C WinAnsi yes yes yes 48 0 AFUUXY+Corbel CID TrueType Identity-H yes yes yes 49 0 SNNFVA+Corbel2 Type 1C WinAnsi yes yes yes 286 0 MinionPro-Bold Type 1C WinAnsi yes no yes 50 0 EuclidSymbol Type 1C Custom yes no yes 51 0 QPZVIS+STIXGeneral-Regular Type 1C Custom yes yes yes 135 0 BaskervilleBT-Bold Type 1C WinAnsi yes no yes 171 0 SABHKO+TimesNewRomanPSMT Type 1C WinAnsi yes yes yes 241 0 Jhove (Rel. 1.22.1, 2019-04-17) Date: 2023-05-05 02:30:50 CEST RepresentationInformation: scidat_a2022v9p90.pdf ReportingModule: PDF-hul, Rel. 1.12.1 (2019-04-17) LastModified: 2023-05-04 12:37:57 CEST Size: 3615338 Format: PDF Version: 1.4 Status: Well-Formed, but not valid SignatureMatches: PDF-hul ID: PDF-HUL-122 ErrorMessage: edu.harvard.hul.ois.jhove.module.pdf.PdfInvalidException: Invalid indirect destination - referenced object 'SpringerNature_Scidata_1161.indd:Fig. 1 Experimental design and sampling procedure. Scintillation vials represent metabolite sampling points whereas microtubes represent RNA sampling points (Days 1 and 5).:64' cannot be found Offset: 3026673 ID: PDF-HUL-122 ErrorMessage: edu.harvard.hul.ois.jhove.module.pdf.PdfInvalidException: Invalid indirect destination - referenced object 'SpringerNature_Scidata_1161.indd: 21. Fàbregas, N. et al. Overexpression of the vascular brassinosteroid receptor BRL3 confers drought resistance without penalizing plant growth. Nat. Commun. 9 (2018). :72' cannot be found Offset: 3026673 ID: PDF-HUL-122 ErrorMessage: edu.harvard.hul.ois.jhove.module.pdf.PdfInvalidException: Invalid indirect destination - referenced object 'SpringerNature_Scidata_1161.indd:Fig. 1 Experimental design and sampling procedure. Scintillation vials represent metabolite sampling points whereas microtubes represent RNA sampling points (Days 1 and 5).:85' cannot be found Offset: 3026673 ID: PDF-HUL-122 ErrorMessage: edu.harvard.hul.ois.jhove.module.pdf.PdfInvalidException: Invalid indirect destination - referenced object 'SpringerNature_Scidata_1161.indd:Fig. 2 Technical validation. (a) Boxplot with the distribution of metabolomic measures (log-scale) per sample. Note the few samples showing an evident outlier distribution. These are likely artifacts and were deleted for subsequent analyses. R:86' cannot be found Offset: 3026673 ID: PDF-HUL-122 ErrorMessage: edu.harvard.hul.ois.jhove.module.pdf.PdfInvalidException: Invalid indirect destination - referenced object 'SpringerNature_Scidata_1161.indd:Fig. 2 Technical validation. (a) Boxplot with the distribution of metabolomic measures (log-scale) per sample. Note the few samples showing an evident outlier distribution. These are likely artifacts and were deleted for subsequent analyses. :87' cannot be found Offset: 3026673 ID: PDF-HUL-122 ErrorMessage: edu.harvard.hul.ois.jhove.module.pdf.PdfInvalidException: Invalid indirect destination - referenced object 'SpringerNature_Scidata_1161.indd: Table 1. Deleted samples, identified as outliers. The overall median for all measurements in the data set (log-scale) is −6.30 and first quartile (Q1, percentil 0.25) and the third quartile (Q3, percentil 0.75) are −8.01 and −:88' cannot be found Offset: 3026673 ID: PDF-HUL-122 ErrorMessage: edu.harvard.hul.ois.jhove.module.pdf.PdfInvalidException: Invalid indirect destination - referenced object 'SpringerNature_Scidata_1161.indd:Fig. 2 Technical validation. (a) Boxplot with the distribution of metabolomic measures (log-scale) per sample. Note the few samples showing an evident outlier distribution. These are likely artifacts and were deleted for subsequent analyses.:89' cannot be found Offset: 3026673 ID: PDF-HUL-122 ErrorMessage: edu.harvard.hul.ois.jhove.module.pdf.PdfInvalidException: Invalid indirect destination - referenced object 'SpringerNature_Scidata_1161.indd:Fig. 2 Technical validation. (a) Boxplot with the distribution of metabolomic measures (log-scale) per sample. Note the few samples showing an evident outlier distribution. These are likely artifacts and were deleted for subsequent analyses:91' cannot be found Offset: 3026673 ID: PDF-HUL-122 ErrorMessage: edu.harvard.hul.ois.jhove.module.pdf.PdfInvalidException: Invalid indirect destination - referenced object 'SpringerNature_Scidata_1161.indd:Fig. 2 Technical validation. (a) Boxplot with the distribution of metabolomic measures (log-scale) per sample. Note the few samples showing an evident outlier distribution. These are likely artifacts and were deleted for subsequent analyse:92' cannot be found Offset: 3026673 ID: PDF-HUL-122 ErrorMessage: edu.harvard.hul.ois.jhove.module.pdf.PdfInvalidException: Invalid indirect destination - referenced object 'SpringerNature_Scidata_1161.indd: 5. Dong, S. & Beckles, D. M. Dynamic changes in the starch-sugar interconversion within plant source and sink tissues promote a better abiotic stress response. J. Plant Physiol. 234–235, 80–93 (2019). :94' cannot be found Offset: 3026673 ID: PDF-HUL-122 ErrorMessage: edu.harvard.hul.ois.jhove.module.pdf.PdfInvalidException: Invalid indirect destination - referenced object 'SpringerNature_Scidata_1161.indd:Fig. 2 Technical validation. (a) Boxplot with the distribution of metabolomic measures (log-scale) per sample. Note the few samples showing an evident outlier distribution. These are likely artifacts and were deleted for subsequent analys:97' cannot be found Offset: 3026673 ID: PDF-HUL-122 ErrorMessage: edu.harvard.hul.ois.jhove.module.pdf.PdfInvalidException: Invalid indirect destination - referenced object 'SpringerNature_Scidata_1161.indd:Fig. 3 Validation of RNAseq. (a) PCA plot of samples based on gene counts (NOISeq R package42). PC1 roughly coincide with drought, which clearly separates samples. PC2 roughly corresponds with the genotype, which also separates well the sa:100' cannot be found Offset: 3026673 ID: PDF-HUL-122 ErrorMessage: edu.harvard.hul.ois.jhove.module.pdf.PdfInvalidException: Invalid indirect destination - referenced object 'SpringerNature_Scidata_1161.indd:Fig. 3 Validation of RNAseq. (a) PCA plot of samples based on gene counts (NOISeq R package42). PC1 roughly coincide with drought, which clearly separates samples. PC2 roughly corresponds with the genotype, which also separates well the s:101' cannot be found Offset: 3026673 ID: PDF-HUL-122 ErrorMessage: edu.harvard.hul.ois.jhove.module.pdf.PdfInvalidException: Invalid indirect destination - referenced object 'SpringerNature_Scidata_1161.indd:Fig. 4 Usage examples: (a) Time 0 comparison between WT and BRL3ox. Boxplots represents the (log) fold-change relative to the WT median. Points are the particular relativized values of each replicate. Important osmoprotectant metabolites app:104' cannot be found Offset: 3026673 ID: PDF-HUL-122 ErrorMessage: edu.harvard.hul.ois.jhove.module.pdf.PdfInvalidException: Invalid indirect destination - referenced object 'SpringerNature_Scidata_1161.indd: Table 1. Deleted samples, identified as outliers. The overall median for all measurements in the data set (log-scale) is −6.30 and first quartile (Q1, percentil 0.25) and the third quartile (Q3, percentil 0.75) are −8.01 and :106' cannot be found Offset: 3026673 ID: PDF-HUL-122 ErrorMessage: edu.harvard.hul.ois.jhove.module.pdf.PdfInvalidException: Invalid indirect destination - referenced object 'SpringerNature_Scidata_1161.indd:Fig. 2 Technical validation. (a) Boxplot with the distribution of metabolomic measures (log-scale) per sample. Note the few samples showing an evident outlier distribution. These are likely artifacts and were deleted for subsequent analy:107' cannot be found Offset: 3026673 ID: PDF-HUL-122 ErrorMessage: edu.harvard.hul.ois.jhove.module.pdf.PdfInvalidException: Invalid indirect destination - referenced object 'SpringerNature_Scidata_1161.indd:Fig. 4 Usage examples: (a) Time 0 comparison between WT and BRL3ox. Boxplots represents the (log) fold-change relative to the WT median. Points are the particular relativized values of each replicate. Important osmoprotectant metabolites a:109' cannot be found Offset: 3026673 ID: PDF-HUL-122 ErrorMessage: edu.harvard.hul.ois.jhove.module.pdf.PdfInvalidException: Invalid indirect destination - referenced object 'SpringerNature_Scidata_1161.indd:Fig. 4 Usage examples: (a) Time 0 comparison between WT and BRL3ox. Boxplots represents the (log) fold-change relative to the WT median. Points are the particular relativized values of each replicate. Important osmoprotectant metabolites :112' cannot be found Offset: 3026673 ID: PDF-HUL-122 ErrorMessage: edu.harvard.hul.ois.jhove.module.pdf.PdfInvalidException: Invalid indirect destination - referenced object 'SpringerNature_Scidata_1161.indd:Fig. 4 Usage examples: (a) Time 0 comparison between WT and BRL3ox. Boxplots represents the (log) fold-change relative to the WT median. Points are the particular relativized values of each replicate. Important osmoprotectant metabolites:113' cannot be found Offset: 3026673 ID: PDF-HUL-122 ErrorMessage: edu.harvard.hul.ois.jhove.module.pdf.PdfInvalidException: Invalid indirect destination - referenced object 'SpringerNature_Scidata_1161.indd:Fig. 5 The dynamic evolution of the calculated Composite Index (CI) for each cluster in Col-0 and BRL3ox genotypes. Each line represents a metabolite cluster. Dashed lines are CI of clusters that are not yielding DNBs. Solid lines are the clus:115' cannot be found Offset: 3026673 ID: PDF-HUL-122 ErrorMessage: edu.harvard.hul.ois.jhove.module.pdf.PdfInvalidException: Invalid indirect destination - referenced object 'SpringerNature_Scidata_1161.indd: Table 2. Identification of DNBs based on metabolites correlation and variance.:116' cannot be found Offset: 3026673 ID: PDF-HUL-122 ErrorMessage: edu.harvard.hul.ois.jhove.module.pdf.PdfInvalidException: Invalid indirect destination - referenced object 'SpringerNature_Scidata_1161.indd: Table 3. Integration of drought metabolomics and transcriptomics. KEGG pathways affected by the differential drought response between BRL3ox and WT roots. Identification of pathways based on the overrepresentation of DEG:120' cannot be found Offset: 3026673 MIMEtype: application/pdf PDFMetadata: Objects: 503 FreeObjects: 1 IncrementalUpdates: 0 DocumentCatalog: ViewerPreferences: HideToolbar: false HideMenubar: false HideWindowUI: false FitWindow: false CenterWindow: false DisplayDocTitle: false NonFullScreenPageMode: UseNone Direction: L2R ViewArea: CropBox ViewClip: CropBox PrintArea: CropBox PageClip: CropBox PageLayout: SinglePage PageMode: UseNone Outlines: Item: Title: Analysis of metabolic dynamics during drought stress in Arabidopsis plants Children: Item: Title: Background & Summary Item: Title: Methods Children: Item: Title: Plant growth and sample collection. Item: Title: Metabolomic data. Children: Item: Title: Metabolite extraction. Item: Title: Metabolite chromatography and detection. Item: Title: Metabolite identification and annotation. Item: Title: Metabolomic data analysis. Item: Title: RNAseq data. Children: Item: Title: RNA extraction, library preparation and sequencing. Item: Title: RNAseq analysis and functional annotation. Item: Title: Data Records Item: Title: Technical Validation Children: Item: Title: Experimental design. Item: Title: Metabolomic samples distribution. Item: Title: Sugars accumulation in later drought stages. Item: Title: Transcriptomic fingerprints. Item: Title: Usage Notes Children: Item: Title: Comparison between genotypes at a given time point. Item: Title: Shoot-to-root metabolite mobilization upon drought. Item: Title: Differential metabolite dynamics upon drought. Item: Title: Identification of Dynamical Network Biomarkers (DNBs) along drought time course. Item: Title: Integration of metabolomics and transcriptomics. Item: Title: Acknowledgements Item: Title: Fig. 1 Experimental design and sampling procedure. Item: Title: Fig. 2 Technical validation. Item: Title: Fig. 3 Validation of RNAseq. Item: Title: Fig. 4 Usage examples: (a) Time 0 comparison between WT and BRL3ox. Item: Title: Fig. 5 The dynamic evolution of the calculated Composite Index (CI) for each cluster in Col-0 and BRL3ox genotypes. Item: Title: Table 1 Deleted samples, identified as outliers. Item: Title: Table 2 Identification of DNBs based on metabolites correlation and variance. Item: Title: Table 3 Integration of drought metabolomics and transcriptomics. 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