8e5ffb229d46f5373f9fc999886fe07d CelBio_a2022v11p73837iENG.pdf eed7a118f9734f411cc868e9385e1aef9cb0e452 CelBio_a2022v11p73837iENG.pdf 7228115414e5015362c3969d943ce01ee709a6f436ea99269b6d86049a6cfe75 CelBio_a2022v11p73837iENG.pdf Creator: Adobe InDesign 15.1 (Windows) Producer: Adobe PDF Library 15.0 CreationDate: Fri Nov 11 17:24:20 2022 CET ModDate: Fri Nov 11 17:24:37 2022 CET Tagged: no UserProperties: no Suspects: no Form: none JavaScript: no Pages: 32 Encrypted: no Page size: 612 x 792 pts (letter) Page rot: 0 File size: 14270893 bytes Optimized: yes PDF version: 1.7 name type encoding emb sub uni object ID ------------------------------------ ----------------- ---------------- --- --- --- --------- SBVUZN+AvenirLTStd-Medium Type 1C WinAnsi yes yes yes 1665 0 SBVUZN+AvenirLTStd-MediumOblique Type 1C Custom yes yes yes 1666 0 SBVUZN+AvenirLTStd-Black Type 1C Custom yes yes yes 1673 0 SBVUZN+AvenirLTStd-BlackOblique Type 1C Custom yes yes yes 1674 0 SBVUZN+AvenirLTStd-Roman Type 1C Custom yes yes yes 1682 0 GJRANL+AvenirLTStd-Oblique Type 1C Custom yes yes yes 1683 0 GJRANL+AvenirLTStd-Heavy Type 1C Custom yes yes yes 1691 0 SBVUZN+AvenirLTStd-Medium Type 1C WinAnsi yes yes yes 42 0 SBVUZN+AvenirLTStd-Medium Type 1C WinAnsi yes yes yes 1425 0 GJRANL+AvenirLTStd-Light Type 1C Custom yes yes yes 1447 0 GJRANL+AvenirLTStd-LightOblique Type 1C WinAnsi yes yes yes 1445 0 GJRANL+AvenirLTStd-BookOblique Type 1C WinAnsi yes yes yes 1451 0 GJRANL+Arial-BoldMT Type 1C WinAnsi yes yes no 1429 0 GJRANL+ArialMT Type 1C WinAnsi yes yes no 1452 0 GJRANL+ArialMT Type 1C WinAnsi yes yes no 1436 0 GJRANL+ArialMT Type 1C WinAnsi yes yes no 1437 0 GJRANL+Arial-ItalicMT Type 1C WinAnsi yes yes no 1434 0 GJRANL+Arial-BoldMT Type 1C WinAnsi yes yes no 1449 0 GJRANL+DejaVuSans Type 1C WinAnsi yes yes no 1431 0 SBVUZN+AvenirLTStd-Medium Type 1C WinAnsi yes yes yes 1440 0 GJRANL+ArialMT CID TrueType Identity-H yes yes yes 1464 0 GJRANL+DejaVuSans-Bold TrueType WinAnsi yes yes yes 1460 0 GJRANL+DejaVuSans TrueType WinAnsi yes yes yes 1459 0 GJRANL+SegoeUI CID TrueType Identity-H yes yes yes 1469 0 SJTUZN+MingLiU-ExtB-ETen-B5-H CID Type 0C Identity-H yes yes no 1488 0 GJRANL+DejaVuSans Type 1C WinAnsi yes yes no 1487 0 IUTMPH+DejaVuSans-Bold Type 1C WinAnsi yes yes no 1482 0 GJRANL+DejaVuSans Type 1C WinAnsi yes yes no 1479 0 IUTMPH+DejaVuSans-Oblique Type 1C WinAnsi yes yes no 1485 0 IUTMPH+DejaVuSans-Bold Type 1C WinAnsi yes yes no 1481 0 IUTMPH+DejaVuSans-BoldOblique Type 1C WinAnsi yes yes no 1480 0 GJRANL+DejaVuSans Type 1C WinAnsi yes yes no 1491 0 IUTMPH+DejaVuSans-Bold Type 1C WinAnsi yes yes no 1492 0 GJRANL+DejaVuSans Type 1C WinAnsi yes yes no 1490 0 IUTMPH+Avenir-BlackOblique CID TrueType Identity-H yes yes yes 1493 0 IUTMPH+SegoeUISymbol CID TrueType Identity-H yes yes yes 1501 0 XBEYRD+MS-Mincho CID TrueType Identity-H yes yes yes 1508 0 MIPFTZ+Avenir-Roman CID TrueType Identity-H yes yes yes 1514 0 MIPFTZ+Avenir-Heavy CID TrueType Identity-H yes yes yes 1519 0 Jhove (Rel. 1.22.1, 2019-04-17) Date: 2023-05-06 02:26:14 CEST RepresentationInformation: CelBio_a2022v11p73837iENG.pdf ReportingModule: PDF-hul, Rel. 1.12.1 (2019-04-17) LastModified: 2023-05-05 14:28:45 CEST Size: 14270893 Format: PDF Version: 1.7 Status: Well-Formed and valid SignatureMatches: PDF-hul MIMEtype: application/pdf Profile: Linearized PDF PDFMetadata: Objects: 1728 FreeObjects: 1 IncrementalUpdates: 1 DocumentCatalog: ViewerPreferences: HideToolbar: false HideMenubar: false HideWindowUI: false FitWindow: false CenterWindow: false DisplayDocTitle: false NonFullScreenPageMode: UseNone Direction: L2R ViewArea: CropBox ViewClip: CropBox PrintArea: CropBox PageClip: CropBox PageLayout: SinglePage PageMode: UseNone Language: en-US Outlines: Item: Title: The Arabidopsis SAC9 enzyme is enriched in a cortical population of early endosomes and restricts PI(4,5)P2 at the plasma membrane Children: Item: Title: Editor's evaluation Item: Title: Introduction Item: Title: Results Children: Item: Title: The cysteine 459 in the catalytic domain of SAC9 is required for SAC9 function Item: Title: SAC9 localizes to a population of early endosomes close to the plasma membrane Item: Title: SAC9 is required to maintain the pool of PI(4,5)P2 at the plasma membrane Item: Title: SAC9 is required for efficient endocytic trafficking Item: Title: SAC9 is required for SH3P2 localization at the plasma membrane Item: Title: The endocytic protein TPLATE has an altered dynamic in the absence of SAC9 Item: Title: Discussion Children: Item: Title: SAC9 and phosphoinositide interconversion along the endocytic pathway Item: Title: Accumulation of PI(4,5)P2-containing vesicles in the sac9 mutant Item: Title: How could SAC9 regulate endocytosis? Item: Title: Limitation of the study Item: Title: Materials and methods Children: Item: Title: Cloning Item: Title: Growth condition and plant materials Item: Title: FM4-64 staining and drug treatments Item: Title: Western blot Item: Title: Transient expression in N. benthamiana Item: Title: Immunoprecipitation Item: Title: Mass spectrometry data analysis Item: Title: NanoLC-MS/MS analysis and data processing Item: Title: Live cell imaging Item: Title: Yeast two-hybrid screen Item: Title: TIRF imaging Item: Title: Dissociation indexes Item: Title: Measures, counting, and statistical analysis Item: Title: Quantification of mCIT-SAC9 compartment densities and colocalization with compartments markers Item: Title: Acknowledgements Item: Title: Additional information Children: Item: Title: Funding Item: Title: Author contributions Item: Title: Author ORCIDs Item: Title: Decision letter and Author response Item: Title: Additional files Children: Item: Title: Supplementary files Item: Title: 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