a063cebcf569db91089d4ac8dc560af6 277694.pdf 35915523a968619a09423646c4c4fb07a127919c 277694.pdf 4955066aa3d3a46aa648088fa084df2708d44c360277e8ce800a20c705e167f6 277694.pdf Title: Identification of Two Eosinophil Subsets in Induced Sputum from Patients with Allergic Asthma According to CD15 and CD66b Expression Subject: Two subsets of eosinophils have been described: resident eosinophils with homeostatic functions (rEOS) in healthy subjects and in patients with nonallergic eosinophilic asthma, and inflammatory eosinophils (iEOS) in blood and lung samples from patients with allergic asthma. We explored if it would be possible to identify different subsets of eosinophils using flow cytometry and the gating strategy applied to induced sputum. We conducted an observational cross-sectional single-center study of 62 patients with persistent allergic asthma. Inflammatory cells from induced sputum samples were counted by light microscopy and flow cytometry, and cytokine levels in the supernatant were determined. Two subsets of eosinophils were defined that we call E1 (CD66b-high and CD15-high) and E2 (CD66b-low and CD15-low). Of the 62 patients, 24 were eosinophilic, 18 mixed, 10 paucigranulocytic, and 10 neutrophilic. E1 predominated over E2 in the eosinophilic and mixed patients (20.86% vs. 6.27% and 14.42% vs. 4.31%, respectively), while E1 and E2 were similar for neutrophilic and paucigranulocytic patients. E1 correlated with IL-5, fractional exhaled nitric oxide, and blood eosinophils. While eosinophil subsets have been identified for asthma in blood, we have shown that they can also be identified in induced sputum. Keywords: allergic asthma; eosinophil subsets; flow cytometry; induced sputum Author: Elena Curto, Éder F. Mateus-Medina, Astrid Crespo-Lessmann, Rubén Osuna-Gómez, Cristina Ujaldón-Miró, Alba García-Moral, Paula Galván-Blasco, Lorena Soto-Retes, David Ramos-Barbón and Vicente Plaza Creator: LaTeX with hyperref Producer: pdfTeX-1.40.21 CreationDate: Tue Oct 25 02:55:10 2022 CEST ModDate: Tue Oct 25 03:25:40 2022 CEST Tagged: no UserProperties: no Suspects: no Form: none JavaScript: no Pages: 10 Encrypted: no Page size: 595.276 x 841.89 pts (A4) Page rot: 0 File size: 975824 bytes Optimized: no PDF version: 1.7 name type encoding emb sub uni object ID ------------------------------------ ----------------- ---------------- --- --- --- --------- HTNSOF+VnURWPalladioL-Bold Type 1 Custom yes yes no 10 0 KVOXWW+VnURWPalladioL Type 1 Custom yes yes no 15 0 UXVNUU+URWPalladioL-Bold Type 1 Custom yes yes no 19 0 ZPOQOV+URWPalladioL-Roma Type 1 Custom yes yes no 24 0 AIPEIL+URWPalladioL-Ital Type 1 Custom yes yes no 28 0 ZXDKIP+EURM10 Type 1 Builtin yes yes no 32 0 LWQXII+TimesNewRomanPS-BoldItalicMT Type 1C WinAnsi yes yes no 50 0 IRWHZX+TimesNewRomanPSMT Type 1C WinAnsi yes yes no 53 0 AHSHLD+CMSY10 Type 1 Builtin yes yes no 78 0 CJMGLJ+PalatinoLinotype,Bold TrueType WinAnsi yes yes no 89 0 CJMGLK+PalatinoLinotype CID TrueType Identity-H yes yes yes 92 0 CJMGLL+PalatinoLinotype TrueType WinAnsi yes yes no 98 0 CJMGLN+PalatinoLinotype,Italic TrueType WinAnsi yes yes no 101 0 CJMHPG+TimesNewRoman CID TrueType Identity-H yes yes yes 104 0 STKLCB+TeX-tipa10 Type 1 Builtin yes yes no 114 0 QLBIEB+URWPalladioL-BoldItal Type 1 Custom yes yes no 118 0 CJMGLJ+PalatinoLinotype,Bold TrueType WinAnsi yes yes no 135 0 CJMGLL+PalatinoLinotype TrueType WinAnsi yes yes no 138 0 CJMGLN+PalatinoLinotype,Italic TrueType WinAnsi yes yes no 141 0 CJMIKE+PalatinoLinotype,BoldItalic TrueType WinAnsi yes yes no 144 0 Jhove (Rel. 1.22.1, 2019-04-17) Date: 2023-07-19 02:37:47 CEST RepresentationInformation: 277694.pdf ReportingModule: PDF-hul, Rel. 1.12.1 (2019-04-17) LastModified: 2023-07-18 12:16:28 CEST Size: 975824 Format: PDF Version: 1.7 Status: Well-Formed and valid SignatureMatches: PDF-hul MIMEtype: application/pdf PDFMetadata: Objects: 289 FreeObjects: 1 IncrementalUpdates: 0 DocumentCatalog: PageLayout: SinglePage PageMode: UseNone Outlines: Item: Title: Introduction Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@44c8afef Item: Title: Materials and Methods Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@7b69c6ba Children: Item: Title: Sputum Induction and Processing Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@46daef40 Item: Title: Flow Cytometry Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@12f41634 Item: Title: Supernatant Cytokine Analysis Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@13c27452 Item: Title: Statistical Analysis Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@262b2c86 Item: Title: Results Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@371a67ec Item: Title: Discussion Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@5ed828d Item: Title: Conclusions Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@50d0686 Item: Title: References Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@7a3d45bd Info: Title: Identification of Two Eosinophil Subsets in Induced Sputum from Patients with Allergic Asthma According to CD15 and CD66b Expression Author: Elena Curto, Éder F. Mateus-Medina, Astrid Crespo-Lessmann, Rubén Osuna-Gómez, Cristina Ujaldón-Miró, Alba García-Moral, Paula Galván-Blasco, Lorena Soto-Retes, David Ramos-Barbón and Vicente Plaza Subject: Two subsets of eosinophils have been described: resident eosinophils with homeostatic functions (rEOS) in healthy subjects and in patients with nonallergic eosinophilic asthma, and inflammatory eosinophils (iEOS) in blood and lung samples from patients with allergic asthma. We explored if it would be possible to identify different subsets of eosinophils using flow cytometry and the gating strategy applied to induced sputum. We conducted an observational cross-sectional single-center study of 62 patients with persistent allergic asthma. Inflammatory cells from induced sputum samples were counted by light microscopy and flow cytometry, and cytokine levels in the supernatant were determined. Two subsets of eosinophils were defined that we call E1 (CD66b-high and CD15-high) and E2 (CD66b-low and CD15-low). Of the 62 patients, 24 were eosinophilic, 18 mixed, 10 paucigranulocytic, and 10 neutrophilic. E1 predominated over E2 in the eosinophilic and mixed patients (20.86% vs. 6.27% and 14.42% vs. 4.31%, respectively), while E1 and E2 were similar for neutrophilic and paucigranulocytic patients. E1 correlated with IL-5, fractional exhaled nitric oxide, and blood eosinophils. While eosinophil subsets have been identified for asthma in blood, we have shown that they can also be identified in induced sputum. 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