520503c2c53244b22981b9481a37da1a 280874.pdf 96ecf0f2597bb8cb18c63d5db673cf5cd6de8050 280874.pdf f12259e2c7e77cfa4dc41058b252a3d66b0b5cb194ac0ed6658063fdb38c77fe 280874.pdf Title: Differential effects of dolutegravir, bictegravir and raltegravir in adipokines and inflammation markers on human adipocytes Subject: Life Sciences, 308 (2022) 120948. doi:10.1016/j.lfs.2022.120948 Keywords: Fat,Adipocyte,Anti-retroviral treatment,HIV integrase inhibitors,Adipokine,Cytokine Author: Pere Domingo Creator: Elsevier Producer: Acrobat Distiller 8.1.0 (Windows) CreationDate: Mon Dec 19 19:04:07 2022 CET ModDate: Tue Dec 20 12:18:03 2022 CET Tagged: yes UserProperties: no Suspects: no Form: none JavaScript: no Pages: 8 Encrypted: no Page size: 595.276 x 793.701 pts Page rot: 0 File size: 748452 bytes Optimized: yes PDF version: 1.7 name type encoding emb sub uni object ID ------------------------------------ ----------------- ---------------- --- --- --- --------- AYCPKX+CharisSIL CID TrueType Identity-H yes yes yes 509 0 FMRPVX+ArialMT CID TrueType Identity-H yes yes yes 514 0 DYDQNN+Univers Type 1C Builtin yes yes yes 519 0 VCGPEB+STIX-Regular Type 1C Custom yes yes yes 523 0 OUTUKK+TeX_CM_Roman Type 1C Builtin yes yes yes 528 0 BQSKOF+CharisSIL-Italic CID TrueType Identity-H yes yes yes 532 0 QLVPKH+CharisSIL CID TrueType Identity-H yes yes yes 500 0 JNNVDT+CharisSIL-Bold CID TrueType Identity-H yes yes yes 207 0 DKIAFF+TeX_CM_Maths_Symbols Type 1C Builtin yes yes yes 212 0 VVCCGG+TeX_CM_Maths_Italic Type 1C Builtin yes yes yes 216 0 TXQHMB+CharisSIL-Italic CID TrueType Identity-H yes yes yes 200 0 FBWZGK+TimesNewRomanPSMT CID TrueType Identity-H yes yes yes 220 0 JDJLFA+ArialMT Type 1C WinAnsi yes yes no 37 0 JDJLEP+Arial-BoldItalicMT Type 1C WinAnsi yes yes no 40 0 JDJLEO+Arial-BoldMT Type 1C WinAnsi yes yes no 43 0 JDLKAC+ArialMT Type 1C WinAnsi yes yes no 54 0 JDLKAB+Arial-BoldMT Type 1C WinAnsi yes yes no 57 0 JDNENO+ArialMT Type 1C WinAnsi yes yes no 79 0 JDNENN+Arial-BoldMT Type 1C WinAnsi yes yes no 82 0 JDNENM+Arial-BoldItalicMT Type 1C WinAnsi yes yes no 85 0 Jhove (Rel. 1.22.1, 2019-04-17) Date: 2023-07-27 02:32:01 CEST RepresentationInformation: 280874.pdf ReportingModule: PDF-hul, Rel. 1.12.1 (2019-04-17) LastModified: 2023-07-26 13:08:10 CEST Size: 748452 Format: PDF Version: 1.7 Status: Well-Formed and valid SignatureMatches: PDF-hul MIMEtype: application/pdf Profile: Linearized PDF, Tagged PDF PDFMetadata: Objects: 614 FreeObjects: 1 IncrementalUpdates: 1 DocumentCatalog: PageLayout: SinglePage PageMode: UseOutlines Language: en Outlines: Item: Title: Differential effects of dolutegravir, bictegravir and raltegravir in adipokines and inflammation markers on human adipocytes Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@74ad1f1f Children: Item: Title: 1 Introduction Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@6a1aab78 Item: Title: 2 Materials and methods Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@462d5aee Children: Item: Title: 2.1 Reagents Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@69b0fd6f Item: Title: 2.2 Human adipocyte cell culture Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@757942a1 Item: Title: 2.3 Assessment of lipid accumulation in adipocytes Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@4a87761d Item: Title: 2.4 RNA isolation, conventional reverse transcription, and quantitative reverse transcription polymerase-chain reaction (qR ... Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@66d1af89 Item: Title: 2.5 Cytotoxicity assays Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@8646db9 Item: Title: 2.6 Protein analysis Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@37374a5e Item: Title: 2.7 Statistical analyses Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@4671e53b Item: Title: 3 Results Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@2db7a79b Children: Item: Title: 3.1 Pre-adipocytes treatment with INsTIs does not elicit cytotoxicity Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@6950e31 Item: Title: 3.2 INsTIs did not alter overall adipogenesis but have differential effects on adipocyte gene expression Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@b7dd107 Item: Title: 3.3 Acute effects of INsTIs on human adipocytes Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@42eca56e Item: Title: 4 Discussion Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@52f759d7 Item: Title: 5 Conclusions Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@7cbd213e Item: Title: CRediT authorship contribution statement Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@192d3247 Item: Title: Declaration of competing interest Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@3ecd23d9 Item: Title: Acknowledgements Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@569cfc36 Item: Title: Appendix A Supplementary data Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@43bd930a Item: Title: References Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@33723e30 Info: Title: Differential effects of dolutegravir, bictegravir and raltegravir in adipokines and inflammation markers on human adipocytes Author: Pere Domingo Subject: Life Sciences, 308 (2022) 120948. doi:10.1016/j.lfs.2022.120948 Keywords: Fat,Adipocyte,Anti-retroviral treatment,HIV integrase inhibitors,Adipokine,Cytokine Creator: Elsevier Producer: Acrobat Distiller 8.1.0 (Windows) CreationDate: Mon Dec 19 19:04:07 CET 2022 ModDate: Tue Dec 20 12:18:03 CET 2022 ID: 0x80230a0f2d4ac2f1838cb20e4187a951, 0x0d7b755cf43437a97102b042233e02eb Filters: FilterPipeline: FlateDecode FilterPipeline: DCTDecode Images: Image: NisoImageMetadata: FormatName: image/jpg CompressionScheme: JPEG ImageWidth: 236 ImageHeight: 298 BitsPerSample: 8 BitsPerSampleUnit: integer Image: NisoImageMetadata: FormatName: image/jpg CompressionScheme: JPEG ImageWidth: 248 ImageHeight: 271 BitsPerSample: 8 BitsPerSampleUnit: integer Fonts: Type0: Font: BaseFont: FMRPVX+ArialMT Encoding: Identity-H ToUnicode: true Font: BaseFont: BQSKOF+CharisSIL-Italic Encoding: Identity-H ToUnicode: true Font: BaseFont: FBWZGK+TimesNewRomanPSMT Encoding: Identity-H ToUnicode: true Font: BaseFont: AYCPKX+CharisSIL Encoding: Identity-H ToUnicode: true Font: BaseFont: JNNVDT+CharisSIL-Bold Encoding: Identity-H ToUnicode: true Type1: Font: BaseFont: OUTUKK+TeX_CM_Roman FontSubset: true FirstChar: 1 LastChar: 128 FontDescriptor: FontName: OUTUKK+TeX_CM_Roman Flags: Symbolic FontBBox: -50, -180, 1000, 720 FontFile3: true ToUnicode: true Font: BaseFont: DKIAFF+TeX_CM_Maths_Symbols FontSubset: true FirstChar: 1 LastChar: 138 FontDescriptor: FontName: DKIAFF+TeX_CM_Maths_Symbols Flags: Symbolic FontBBox: -50, -180, 1000, 720 FontFile3: true ToUnicode: true Font: BaseFont: DYDQNN+Univers FontSubset: true FirstChar: 32 LastChar: 251 FontDescriptor: FontName: DYDQNN+Univers Flags: Symbolic FontBBox: -50, -180, 1000, 720 FontFile3: true ToUnicode: true Font: BaseFont: VVCCGG+TeX_CM_Maths_Italic FontSubset: true FirstChar: 1 LastChar: 127 FontDescriptor: FontName: VVCCGG+TeX_CM_Maths_Italic Flags: Symbolic FontBBox: -50, -180, 1000, 720 FontFile3: true ToUnicode: true Font: BaseFont: VCGPEB+STIX-Regular FontSubset: true FirstChar: 1 LastChar: 8 FontDescriptor: FontName: VCGPEB+STIX-Regular Flags: Nonsymbolic FontBBox: -970, -486, 2208, 1023 FontFile3: true EncodingDictionary: BaseEncoding: WinAnsiEncoding Differences: true ToUnicode: true CIDFontType2: Font: BaseFont: JNNVDT+CharisSIL-Bold CIDSystemInfo: Registry: Adobe Registry: Identity Supplement: 0 FontDescriptor: FontName: JNNVDT+CharisSIL-Bold Flags: Nonsymbolic, ForceBold FontBBox: -720, -533, 1759, 1320 FontFile2: true Font: BaseFont: FMRPVX+ArialMT CIDSystemInfo: Registry: Adobe Registry: Identity Supplement: 0 FontDescriptor: FontName: FMRPVX+ArialMT Flags: Nonsymbolic FontBBox: -665, -325, 2000, 1040 FontFile2: true Font: BaseFont: BQSKOF+CharisSIL-Italic CIDSystemInfo: Registry: Adobe Registry: Identity Supplement: 0 FontDescriptor: FontName: BQSKOF+CharisSIL-Italic Flags: Nonsymbolic, Italic FontBBox: -716, -533, 1627, 1318 FontFile2: true Font: BaseFont: FBWZGK+TimesNewRomanPSMT CIDSystemInfo: Registry: Adobe Registry: Identity Supplement: 0 FontDescriptor: FontName: FBWZGK+TimesNewRomanPSMT Flags: Nonsymbolic FontBBox: -568, -307, 2000, 1007 FontFile2: true Font: BaseFont: AYCPKX+CharisSIL CIDSystemInfo: Registry: Adobe Registry: Identity Supplement: 0 FontDescriptor: FontName: AYCPKX+CharisSIL Flags: Nonsymbolic FontBBox: -692, -533, 1623, 1319 FontFile2: true XMP: application/pdf 10.1016/j.lfs.2022.120948 Elsevier Inc. Life Sciences, 308 (2022) 120948. doi:10.1016/j.lfs.2022.120948 Fat Adipocyte Anti-retroviral treatment HIV integrase inhibitors Adipokine Cytokine Differential effects of dolutegravir, bictegravir and raltegravir in adipokines and inflammation markers on human adipocytes Pere Domingo Tania Quesada-López Joan Villarroya Montserrat Cairó Maria Del Mar Gutierrez Maria Gracia Mateo Isabel Mur Noemí Corbacho Joan Carles Domingo Francesc Villarroya Marta Giralt journal Life Sciences © 2022 The Authors. Published by Elsevier Inc. 0024-3205 308 1 November 2022 2022-11-01 120948 120948 10.1016/j.lfs.2022.120948 https://doi.org/10.1016/j.lfs.2022.120948 2010-04-23 true 10.1016/j.lfs.2022.120948 elsevier.com sciencedirect.com http://creativecommons.org/licenses/by/4.0/ VoR 10.1016/j.lfs.2022.120948 noindex 2010-04-23 true 20th December 2022 sciencedirect.com elsevier.com Elsevier 2022-12-20T11:18:03 2022-12-19T18:04:07 2022-12-20T11:18:03 True 20th December 2022 Acrobat Distiller 8.1.0 (Windows) Fat,Adipocyte,Anti-retroviral treatment,HIV integrase inhibitors,Adipokine,Cytokine uuid:1b499bed-4ce8-4c0c-b682-0d58baae1cbe uuid:b6ed5266-03cb-4855-90c0-636283357f42 Pages: Page: Label: 1 Annotations: Annotation: Subtype: Link Rect: 106, 576, 112, 587 Flags: Print Destination: 485 Annotation: Subtype: Link Rect: 113, 576, 119, 587 Flags: Print Destination: 485 Annotation: Subtype: Link Rect: 120, 571, 233, 582 Flags: Print Destination: 485 Annotation: Subtype: Link Rect: 233, 576, 239, 587 Flags: Print Destination: 485 Annotation: Subtype: Link Rect: 240, 576, 247, 587 Flags: Print Destination: 485 Annotation: Subtype: Link Rect: 247, 576, 253, 587 Flags: Print Destination: 485 Annotation: Subtype: Link Rect: 254, 576, 261, 587 Flags: Print Destination: 485 Annotation: Subtype: Link Rect: 261, 576, 268, 587 Flags: Print Destination: 485 Annotation: Subtype: Link Rect: 268, 576, 273, 587 Flags: Print Destination: 485 Annotation: Subtype: Link Rect: 274, 571, 359, 582 Flags: Print Destination: 485 Annotation: Subtype: Link Rect: 359, 576, 365, 587 Flags: Print Destination: 485 Annotation: Subtype: Link Rect: 366, 576, 373, 587 Flags: Print Destination: 485 Annotation: Subtype: Link Rect: 373, 576, 379, 587 Flags: Print Destination: 485 Annotation: Subtype: Link Rect: 380, 571, 386, 582 Flags: Print Destination: 485 Annotation: Subtype: Link Rect: 119, 563, 125, 574 Flags: Print Destination: 485 Annotation: Subtype: Link Rect: 125, 563, 132, 574 Flags: Print Destination: 485 Annotation: Subtype: Link Rect: 133, 563, 139, 574 Flags: Print Destination: 485 Annotation: Subtype: Link Rect: 140, 558, 266, 569 Flags: Print Destination: 485 Annotation: Subtype: Link Rect: 266, 563, 273, 574 Flags: Print Destination: 485 Annotation: Subtype: Link Rect: 273, 558, 380, 569 Flags: Print Destination: 485 Annotation: Subtype: Link Rect: 380, 563, 386, 574 Flags: Print Destination: 485 Annotation: Subtype: Link Rect: 387, 558, 450, 569 Flags: Print Destination: 485 Annotation: Subtype: Link Rect: 450, 563, 456, 574 Flags: Print Destination: 485 Annotation: Subtype: Link Rect: 457, 558, 464, 569 Flags: Print Destination: 485 Annotation: Subtype: Link Rect: 118, 550, 124, 561 Flags: Print Destination: 485 Annotation: Subtype: Link Rect: 125, 545, 238, 556 Flags: Print Destination: 485 Annotation: Subtype: Link Rect: 238, 550, 244, 561 Flags: Print Destination: 485 Annotation: Subtype: Link Rect: 244, 545, 348, 556 Flags: Print Destination: 485 Annotation: Subtype: Link Rect: 348, 550, 354, 561 Flags: Print Destination: 485 Annotation: Subtype: Link Rect: 355, 550, 361, 561 Flags: Print Destination: 485 Annotation: Subtype: Link Rect: 362, 550, 368, 561 Flags: Print Destination: 485 Annotation: Subtype: Link Rect: 369, 545, 438, 556 Flags: Print Destination: 485 Annotation: Subtype: Link Rect: 438, 550, 444, 561 Flags: Print Destination: 485 Annotation: Subtype: Link Rect: 444, 550, 451, 561 Flags: Print Destination: 485 Annotation: Subtype: Link Rect: 452, 550, 458, 561 Flags: Print Destination: 485 Annotation: Subtype: Link Rect: 459, 550, 463, 561 Flags: Print Destination: 485 Annotation: Subtype: Link Rect: 464, 553, 468, 558 Flags: Print Destination: 485 Annotation: Subtype: Link Rect: 464, 545, 468, 556 Flags: Print Destination: 485 Annotation: Subtype: Link Rect: 98, 114, 149, 121 Flags: Print Annotation: Subtype: Link Rect: 321, 722, 369, 730 Flags: Print Annotation: Subtype: Link Rect: 274, 667, 397, 675 Flags: Print Annotation: Subtype: Link Rect: 37, 87, 174, 94 Flags: Print Annotation: Subtype: Link Rect: 297, 749, 300, 757 Flags: Print Annotation: Subtype: Link Rect: 244, 748, 349, 760 Annotation: Subtype: Link Rect: 499, 616, 528, 645 Annotation: Subtype: Link Rect: 405, 57, 552, 69 Page: Label: 2 Annotations: Annotation: Subtype: Link Rect: 266, 636, 286, 644 Flags: Print Destination: 113 Annotation: Subtype: Link Rect: 99, 605, 117, 613 Flags: Print Destination: 113 Annotation: Subtype: Link Rect: 195, 542, 206, 550 Flags: Print Destination: 113 Annotation: Subtype: Link Rect: 63, 437, 74, 445 Flags: Print Destination: 113 Annotation: Subtype: Link Rect: 233, 375, 257, 383 Flags: Print Destination: 113 Annotation: Subtype: Link Rect: 306, 730, 322, 738 Flags: Print Destination: 113 Annotation: Subtype: Link Rect: 451, 720, 479, 728 Flags: Print Destination: 113 Annotation: Subtype: Link Rect: 358, 699, 385, 707 Flags: Print Destination: 113 Annotation: Subtype: Link Rect: 445, 238, 461, 246 Flags: Print Destination: 113 Page: Label: 3 Annotations: Annotation: Subtype: Link Rect: 489, 452, 516, 460 Flags: Print Destination: 113 Page: Label: 4 Page: Label: 5 Annotations: Annotation: Subtype: Link Rect: 253, 277, 269, 285 Flags: Print Destination: 113 Annotation: Subtype: Link Rect: 84, 256, 111, 264 Flags: Print Destination: 113 Annotation: Subtype: Link Rect: 71, 113, 98, 121 Flags: Print Destination: 16 Annotation: Subtype: Link Rect: 40, 81, 60, 89 Flags: Print Destination: 16 Annotation: Subtype: Link Rect: 78, 81, 104, 89 Flags: Print Destination: 16 Annotation: Subtype: Link Rect: 483, 266, 503, 274 Flags: Print Destination: 29 Annotation: Subtype: Link Rect: 459, 245, 475, 253 Flags: Print Destination: 113 Annotation: Subtype: Link Rect: 395, 141, 416, 149 Flags: Print Destination: 29 Annotation: Subtype: Link Rect: 485, 99, 506, 107 Flags: Print Destination: 46 Annotation: Subtype: Link Rect: 475, 68, 495, 76 Flags: Print Destination: 46 Page: Label: 6 Page: Label: 7 Annotations: Annotation: Subtype: Link Rect: 185, 730, 205, 738 Flags: Print Destination: 46 Annotation: Subtype: Link Rect: 104, 657, 119, 665 Flags: Print Destination: 113 Annotation: Subtype: Link Rect: 40, 583, 60, 591 Flags: Print Destination: 71 Annotation: Subtype: Link Rect: 259, 447, 286, 455 Flags: Print Destination: 113 Annotation: Subtype: Link Rect: 218, 416, 234, 424 Flags: Print Destination: 113 Annotation: Subtype: Link Rect: 225, 374, 241, 382 Flags: Print Destination: 113 Annotation: Subtype: Link Rect: 143, 332, 159, 340 Flags: Print Destination: 113 Annotation: Subtype: Link Rect: 263, 322, 278, 330 Flags: Print Destination: 113 Annotation: Subtype: Link Rect: 157, 301, 173, 309 Flags: Print Destination: 113 Annotation: Subtype: Link Rect: 243, 217, 254, 225 Flags: Print Destination: 113 Annotation: Subtype: Link Rect: 150, 154, 177, 162 Flags: Print Destination: 113 Annotation: Subtype: Link Rect: 161, 92, 177, 100 Flags: Print Destination: 113 Annotation: Subtype: Link Rect: 352, 689, 368, 696 Flags: Print Destination: 113 Annotation: Subtype: Link Rect: 424, 647, 440, 655 Flags: Print Destination: 113 Annotation: Subtype: Link Rect: 352, 155, 443, 163 Flags: Print Annotation: Subtype: Link Rect: 516, 103, 557, 111 Flags: Print Annotation: Subtype: Link Rect: 306, 92, 417, 100 Flags: Print Page: Label: 8 Annotations: Annotation: Subtype: Link Rect: 53, 712, 279, 719 Flags: Print Annotation: Subtype: Link Rect: 53, 704, 220, 711 Flags: Print Annotation: Subtype: Link Rect: 127, 665, 247, 671 Flags: Print Annotation: Subtype: Link Rect: 163, 625, 265, 631 Flags: Print Annotation: Subtype: Link Rect: 126, 601, 253, 607 Flags: Print Annotation: Subtype: Link Rect: 210, 585, 282, 591 Flags: Print Annotation: Subtype: Link Rect: 53, 577, 86, 583 Flags: Print Annotation: Subtype: Link Rect: 194, 553, 266, 559 Flags: Print Annotation: Subtype: Link Rect: 53, 545, 93, 551 Flags: Print Annotation: Subtype: Link Rect: 182, 513, 275, 520 Flags: Print Annotation: Subtype: Link Rect: 53, 505, 75, 512 Flags: Print Annotation: Subtype: Link Rect: 53, 457, 180, 464 Flags: Print Annotation: Subtype: Link Rect: 236, 425, 282, 432 Flags: Print Annotation: Subtype: Link Rect: 53, 417, 145, 424 Flags: Print Annotation: Subtype: Link Rect: 133, 378, 260, 384 Flags: Print Annotation: Subtype: Link Rect: 123, 354, 250, 360 Flags: Print Annotation: Subtype: Link Rect: 82, 330, 186, 336 Flags: Print Annotation: Subtype: Link Rect: 93, 298, 205, 304 Flags: Print Annotation: Subtype: Link Rect: 190, 266, 265, 272 Flags: Print Annotation: Subtype: Link Rect: 54, 258, 115, 264 Flags: Print Annotation: Subtype: Link Rect: 240, 242, 285, 249 Flags: Print Annotation: Subtype: Link Rect: 54, 234, 137, 241 Flags: Print Annotation: Subtype: Link Rect: 82, 210, 218, 217 Flags: Print Annotation: Subtype: Link Rect: 435, 724, 548, 730 Flags: Print Annotation: Subtype: Link Rect: 400, 684, 513, 690 Flags: Print Annotation: Subtype: Link Rect: 323, 652, 453, 658 Flags: Print Annotation: Subtype: Link Rect: 496, 628, 542, 635 Flags: Print Annotation: Subtype: Link Rect: 323, 620, 404, 627 Flags: Print Annotation: Subtype: Link Rect: 358, 572, 472, 579 Flags: Print Annotation: Subtype: Link Rect: 426, 541, 553, 547 Flags: Print Annotation: Subtype: Link Rect: 323, 533, 555, 539 Flags: Print Annotation: Subtype: Link Rect: 323, 525, 548, 531 Flags: Print Annotation: Subtype: Link Rect: 323, 517, 548, 523 Flags: Print Annotation: Subtype: Link Rect: 323, 509, 432, 515 Flags: Print Annotation: Subtype: Link Rect: 435, 461, 541, 467 Flags: Print Annotation: Subtype: Link Rect: 429, 413, 536, 419 Flags: Print Annotation: Subtype: Link Rect: 496, 389, 541, 396 Flags: Print Annotation: Subtype: Link Rect: 323, 381, 385, 388 Flags: Print Annotation: Subtype: Link Rect: 493, 349, 538, 356 Flags: Print Annotation: Subtype: Link Rect: 323, 341, 395, 348 Flags: Print Annotation: Subtype: Link Rect: 407, 325, 552, 332 Flags: Print Annotation: Subtype: Link Rect: 510, 294, 555, 300 Flags: Print Annotation: Subtype: Link Rect: 323, 286, 381, 292 Flags: Print Annotation: Subtype: Link Rect: 468, 262, 540, 268 Flags: Print Annotation: Subtype: Link Rect: 323, 254, 348, 260 Flags: Print Annotation: Subtype: Link Rect: 496, 222, 541, 228 Flags: Print Annotation: Subtype: Link Rect: 323, 214, 414, 220 Flags: Print Checksum: 10b9465b Type: CRC32 Checksum: 520503c2c53244b22981b9481a37da1a Type: MD5 Checksum: 96ecf0f2597bb8cb18c63d5db673cf5cd6de8050 Type: SHA-1