234bcf81278b247083610df1ac34bdf4 283193.pdf d986776cda3049330715602bcd39746af8f8a86c 283193.pdf 830b23bf0aed7846c0a4f4e678ba632ffec485a155328910f0aa45170909d06d 283193.pdf Title: Lipoprotein Profile in Immunological Non-Responders PLHIV after Antiretroviral Therapy Initiation Subject: Nuclear magnetic resonance (NMR)-based advanced lipoprotein tests have demonstrated that LDL and HDL particle numbers (LDL-P and HDL-P) are more powerful cardiovascular (CV) risk biomarkers than conventional cholesterol markers. Of interest, in people living with HIV (PLHIV), predictors of preclinical atherosclerosis and vascular dysfunction may be associated with impaired immune function. We previously stated that immunological non-responders (INR) were at higher CV risk than immunological responders (IR) before starting antiretroviral therapy (ART). Using Liposcale® tests, we characterized the lipoprotein profile from the same cohort of PLHIV at month 12 and month 36 after starting ART, intending to explore what happened with these indicators of CV risk during viral suppression. ART initiation dissipates the differences in lipoprotein-based CV risk markers between INR and IR, and only an increase in the number of HDL-P was found in INR + IR when compared to controls (p = 0.047). Interestingly, CD4+ T-cell counts negatively correlated with medium HDL-P concentrations at month 12 in all individuals ( = -0.335, p = 0.003). Longitudinal analyses showed an important increase in LDL-P and HDL-P at month 36 when compared to baseline values in both IR and INR. A proper balance between a proatherogenic and atherogenic environment may be related to the reconstitution of CD4+ T-cell count in PLHIV. Keywords: antiretroviral therapy; cardiovascular risk; immunological non-responder; lipoproteins; longitudinal analysis; nuclear magnetic resonance; people living with HIV Author: Jenifer Masip, Rosa Jorba, Miguel López-Dupla, Pere Domingo, Yolanda María Pacheco, Graciano García-Pardo, Esteban Martínez, Consuelo Viladés, Sergi Veloso, Verónica Alba, Montserrat Olona, Francesc Vidal, Frederic Gómez-Bertomeu, Joaquim Peraire and Anna Rull Creator: LaTeX with hyperref Producer: pdfTeX-1.40.21 CreationDate: Fri Jul 22 11:22:55 2022 CEST ModDate: Fri Jul 22 11:26:43 2022 CEST Tagged: no UserProperties: no Suspects: no Form: none JavaScript: no Pages: 12 Encrypted: no Page size: 595.276 x 841.89 pts (A4) Page rot: 0 File size: 3196926 bytes Optimized: no PDF version: 1.7 name type encoding emb sub uni object ID ------------------------------------ ----------------- ---------------- --- --- --- --------- XWOCXD+VnURWPalladioL-Bold Type 1 Custom yes yes no 10 0 GOLQHX+EURM10 Type 1 Builtin yes 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MIMEtype: application/pdf PDFMetadata: Objects: 503 FreeObjects: 1 IncrementalUpdates: 0 DocumentCatalog: PageLayout: SinglePage PageMode: UseNone Outlines: Item: Title: Introduction Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@262b2c86 Item: Title: Results Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@371a67ec Children: Item: Title: Stable ART Diminishes Differences in Lipoprotein-Based Cardiovascular Risk Markers Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@5ed828d Item: Title: HDL Particle Size Correlates with CD4+ T-Cell Count at Month 12 (ART) Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@50d0686 Item: Title: Lipoprotein Profile Evolution in PLHIV Based on the 2D 1H-NMR Liposcale Test Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@7a3d45bd Children: Item: Title: Lipoprotein Profile Evolution in PLHIV Starting ART with More than 350 CD4+ T-Cells/L Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@1e7c7811 Item: Title: Lipoprotein Profile Evolution in PLHIV Starting ART with Less than 200 CD4+ T-Cells/L Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@77ec78b9 Item: Title: Discussion Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@1a3869f4 Item: Title: Materials and Methods Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@a38d7a3 Children: Item: Title: Study Design and Participants Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@77f99a05 Item: Title: Laboratory Measurements Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@63440df3 Item: Title: D 1H-NMR Liposcale Test Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@3aeaafa6 Item: Title: Statistical Analysis Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@76a3e297 Item: Title: Conclusions Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@4d3167f4 Item: Title: References Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@ed9d034 Info: Title: Lipoprotein Profile in Immunological Non-Responders PLHIV after Antiretroviral Therapy Initiation Author: Jenifer Masip, Rosa Jorba, Miguel López-Dupla, Pere Domingo, Yolanda María Pacheco, Graciano García-Pardo, Esteban Martínez, Consuelo Viladés, Sergi Veloso, Verónica Alba, Montserrat Olona, Francesc Vidal, Frederic Gómez-Bertomeu, Joaquim Peraire and Anna Rull Subject: Nuclear magnetic resonance (NMR)-based advanced lipoprotein tests have demonstrated that LDL and HDL particle numbers (LDL-P and HDL-P) are more powerful cardiovascular (CV) risk biomarkers than conventional cholesterol markers. Of interest, in people living with HIV (PLHIV), predictors of preclinical atherosclerosis and vascular dysfunction may be associated with impaired immune function. We previously stated that immunological non-responders (INR) were at higher CV risk than immunological responders (IR) before starting antiretroviral therapy (ART). Using Liposcale® tests, we characterized the lipoprotein profile from the same cohort of PLHIV at month 12 and month 36 after starting ART, intending to explore what happened with these indicators of CV risk during viral suppression. ART initiation dissipates the differences in lipoprotein-based CV risk markers between INR and IR, and only an increase in the number of HDL-P was found in INR + IR when compared to controls (p = 0.047). Interestingly, CD4+ T-cell counts negatively correlated with medium HDL-P concentrations at month 12 in all individuals ( = -0.335, p = 0.003). Longitudinal analyses showed an important increase in LDL-P and HDL-P at month 36 when compared to baseline values in both IR and INR. A proper balance between a proatherogenic and atherogenic environment may be related to the reconstitution of CD4+ T-cell count in PLHIV. 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