af7bab57236e5543d89761872ca9da15 284173.pdf 1cff644bd3a557f81a7e747c9407e67da1c3fb3a 284173.pdf afd73dd03aaa2dd1677f64d26e22cb12718d27dde653ae22728002a52426a7c6 284173.pdf Title: The Weak Relationship between Vitamin D Compounds and Glucose Homeostasis Measures in Pregnant Women with Obesity: An Exploratory Sub-Analysis of the DALI Study Subject: Studies on the relationship between vitamin D (VitD) and glucose homeostasis usually consider either total VitD or 25OHD3 but not 25OHD2 and epimers. We aimed to evaluate the cross-sectional association of VitD compounds with glucose homeostasis measurements in pregnant women with overweight/obesity participating in the Vitamin D And Lifestyle Intervention for Gestational Diabetes Mellitus Prevention study. Methods: The analysis included 912 women. Inclusion criteria: <20 weeks gestation, body mass index 29 kg/m2 and information on exposure and outcome variables at baseline. Measurements: A 75 g OGTT at <20, 24–28 and 35–37 weeks gestation (except if previous diabetes diagnosis). Exposure variables: 25OHD2, 25OHD3 and C3-epimer. Outcome variables: fasting and post-challenge insulin sensitivity and secretion indices, corresponding disposition indices (DI), plasma glucose at fasting and 1 and 2 h, hyperglycemia in pregnancy (HiP). Statistics: Multivariate regression analyses with adjustment. Results: Baseline VitD sufficiency was 66.3%. Overall, VitD compounds did not show strong associations with any glucose homeostasis measures. 25OHD3 showed direct significant associations with: FPG at <20 and 24–28 weeks (standardized coefficient () 0.124, p = 0.030 and 0.111, p = 0.026 respectively), 2 h plasma glucose at 24–28 weeks ( 0.120, p = 0.018), and insulin sensitivity (1/HOMA-IR, 0.127, p = 0.027) at 35–37 weeks; it showed an inverse association with fasting DI (QUCKI*HOMA-) at <20 and 24–28 weeks ( -0.124, p = 0.045 and -0.148, p = 0.004 respectively). 25OHD2 showed direct associations with post-challenge insulin sensitivity (Matsuda, 0.149, p = 0.048) at 24–28 weeks) and post-challenge DI (Matsuda*Stumvoll phase 1) at 24–28 and 35–37 weeks ( 0.168, p = 0.030, 0.239, p = 0.006). No significant association with C3-epimer was observed at any time period. Conclusions: In these women with average baseline VitD in sufficiency range, VitD compounds did not show clear beneficial associations with glucose homeostasis measures. Keywords: vitamin D compounds; 25OHD2; 25OHD3; C3-epimer; glucose homeostasis; pregnancy; obesity Author: Lilian Cristina Mendoza, Jürgen Harreiter, Gernot Desoye, David Simmons, Juan M. Adelantado, Alexandra Kautzky-Willer, Agnieszka Zawiejska, Ewa Wender-Ozegowska, Annunziata Lapolla, Maria G. Dalfra, Alessandra Bertolotto, Roland Devlieger, Fidelma Dunne, Elisabeth R. Mathiesen, Peter Damm, Lisse Lotte Andersen, Dorte Moller Jensen, David Hill, Mireille Nicoline Maria van Poppel and Rosa Corcoy Creator: LaTeX with hyperref Producer: pdfTeX-1.40.21 CreationDate: Tue Aug 9 14:27:29 2022 CEST ModDate: Tue Aug 9 14:34:51 2022 CEST Tagged: no UserProperties: no Suspects: no Form: none JavaScript: no Pages: 13 Encrypted: no Page size: 595.276 x 841.89 pts (A4) Page rot: 0 File size: 584651 bytes Optimized: no PDF version: 1.7 name type encoding emb sub uni object ID ------------------------------------ ----------------- ---------------- --- --- --- --------- PSUYZB+VnURWPalladioL Type 1 Custom yes yes no 10 0 VVSYYB+EURM10 Type 1 Builtin yes yes no 15 0 TAPTQP+URWPalladioL-Bold Type 1 Custom yes yes no 19 0 WKASHO+URWPalladioL-Roma Type 1 Custom yes yes no 24 0 UQPYOH+URWPalladioL-Ital Type 1 Custom yes yes no 28 0 LJEZBI+CMSY10 Type 1 Builtin yes yes no 32 0 HGMNRX+TimesNewRomanPS-BoldItalicMT Type 1C WinAnsi yes yes no 50 0 ZYYJXI+EURB10 Type 1 Builtin yes yes no 90 0 Jhove (Rel. 1.22.1, 2019-04-17) Date: 2023-11-11 02:32:44 CET RepresentationInformation: 284173.pdf ReportingModule: PDF-hul, Rel. 1.12.1 (2019-04-17) LastModified: 2023-11-10 12:15:36 CET Size: 584651 Format: PDF Version: 1.7 Status: Well-Formed and valid SignatureMatches: PDF-hul MIMEtype: application/pdf PDFMetadata: Objects: 321 FreeObjects: 1 IncrementalUpdates: 0 DocumentCatalog: PageLayout: SinglePage PageMode: UseNone Outlines: Item: Title: Introduction Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@4d3167f4 Item: Title: Materials and Methods Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@ed9d034 Children: Item: Title: Study Population Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@6121c9d6 Item: Title: Data Collection and Assessments Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@87f383f Item: Title: Measurements Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@4eb7f003 Item: Title: Statistical Analysis Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@eafc191 Item: Title: Results Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@612fc6eb Item: Title: Discussion Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@1060b431 Item: Title: Conclusions Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@612679d6 Item: Title: References Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@11758f2a Info: Title: The Weak Relationship between Vitamin D Compounds and Glucose Homeostasis Measures in Pregnant Women with Obesity: An Exploratory Sub-Analysis of the DALI Study Author: Lilian Cristina Mendoza, Jürgen Harreiter, Gernot Desoye, David Simmons, Juan M. Adelantado, Alexandra Kautzky-Willer, Agnieszka Zawiejska, Ewa Wender-Ozegowska, Annunziata Lapolla, Maria G. Dalfra, Alessandra Bertolotto, Roland Devlieger, Fidelma Dunne, Elisabeth R. Mathiesen, Peter Damm, Lisse Lotte Andersen, Dorte Moller Jensen, David Hill, Mireille Nicoline Maria van Poppel and Rosa Corcoy Subject: Studies on the relationship between vitamin D (VitD) and glucose homeostasis usually consider either total VitD or 25OHD3 but not 25OHD2 and epimers. We aimed to evaluate the cross-sectional association of VitD compounds with glucose homeostasis measurements in pregnant women with overweight/obesity participating in the Vitamin D And Lifestyle Intervention for Gestational Diabetes Mellitus Prevention study. Methods: The analysis included 912 women. Inclusion criteria: <20 weeks gestation, body mass index 29 kg/m2 and information on exposure and outcome variables at baseline. Measurements: A 75 g OGTT at <20, 24 28 and 35 37 weeks gestation (except if previous diabetes diagnosis). Exposure variables: 25OHD2, 25OHD3 and C3-epimer. Outcome variables: fasting and post-challenge insulin sensitivity and secretion indices, corresponding disposition indices (DI), plasma glucose at fasting and 1 and 2 h, hyperglycemia in pregnancy (HiP). Statistics: Multivariate regression analyses with adjustment. Results: Baseline VitD sufficiency was 66.3%. Overall, VitD compounds did not show strong associations with any glucose homeostasis measures. 25OHD3 showed direct significant associations with: FPG at <20 and 24 28 weeks (standardized coefficient () 0.124, p = 0.030 and 0.111, p = 0.026 respectively), 2 h plasma glucose at 24 28 weeks ( 0.120, p = 0.018), and insulin sensitivity (1/HOMA-IR, 0.127, p = 0.027) at 35 37 weeks; it showed an inverse association with fasting DI (QUCKI*HOMA-) at <20 and 24 28 weeks ( -0.124, p = 0.045 and -0.148, p = 0.004 respectively). 25OHD2 showed direct associations with post-challenge insulin sensitivity (Matsuda, 0.149, p = 0.048) at 24 28 weeks) and post-challenge DI (Matsuda*Stumvoll phase 1) at 24 28 and 35 37 weeks ( 0.168, p = 0.030, 0.239, p = 0.006). No significant association with C3-epimer was observed at any time period. Conclusions: In these women with average baseline VitD in sufficiency range, VitD compounds did not show clear beneficial associations with glucose homeostasis measures. Keywords: vitamin D compounds; 25OHD2; 25OHD3; C3-epimer; glucose homeostasis; pregnancy; obesity Creator: LaTeX with hyperref Producer: pdfTeX-1.40.21 CreationDate: Tue Aug 09 14:27:29 CEST 2022 ModDate: Tue Aug 09 14:34:51 CEST 2022 ID: 0xbaa74c40bd42f7ba58367f78a151fc73, 0xbaa74c40bd42f7ba58367f78a151fc73 Filters: FilterPipeline: FlateDecode Fonts: Type1: Font: BaseFont: LJEZBI+CMSY10 FontSubset: true FirstChar: 0 LastChar: 21 FontDescriptor: FontName: LJEZBI+CMSY10 Flags: Symbolic FontBBox: -29, -960, 1116, 775 FontFile: true Font: BaseFont: TAPTQP+URWPalladioL-Bold FontSubset: true FirstChar: 2 LastChar: 252 FontDescriptor: FontName: TAPTQP+URWPalladioL-Bold Flags: Symbolic FontBBox: -152, -301, 1000, 935 FontFile: true EncodingDictionary: Differences: true Font: BaseFont: WKASHO+URWPalladioL-Roma FontSubset: true FirstChar: 2 LastChar: 248 FontDescriptor: FontName: WKASHO+URWPalladioL-Roma Flags: Symbolic FontBBox: -166, -283, 1021, 943 FontFile: true EncodingDictionary: 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