7b555b386dd377cbafafc1220ac75a63 284193.pdf 629dd20967b5bc142a2bf6721b9dff8e6f07ccae 284193.pdf 0a205a843e12f38d82b59fedb83f77349233c25877e89f843b78bc8b150318d9 284193.pdf Title: Semaphorin-3F/Neuropilin-2 Transcriptional Expression as a Predictive Biomarker of Occult Lymph Node Metastasesin HNSCC Subject: The expression of the semaphorin-3F (SEMA3F) and neuropilin-2 (NRP2) is involved in the regulation of lymphangiogenesis. The present study analyzes the relationship between the transcriptional expression of the SEMA3F-NRP2 genes and the presence of occult lymph node metastases in patients with cN0 head and neck squamous cell carcinomas. We analyzed the transcriptional expression of SEMA3F and NRP2 in a cohort of 53 patients with cN0 squamous cell carcinoma treated with an elective neck dissection. Occult lymph node metastases were found in 37.7% of the patients. Patients with occult lymph node metastases (cN0/pN+) had significantly lower SEMA3F expression values than patients without lymph node involvement (cN0/pN0). Considering the expression of the SEMA3F-NRP2 genes, patients were classified into two groups according to the risk of occult nodal metastasis: Group 1 (n = 34), high SEMA3F/low NRP2 expression, with a low risk of occult nodal involvement (14.7% cN0/pN+); Group 2 (n = 19), low SEMA3F or high SEMA3F/high NRP2 expression, with a high risk of occult nodal involvement (78.9% cN0/pN+). Multivariate analysis showed that patients in Group 2 had a 26.2 higher risk of lymph node involvement than patients in Group 1. There was a significant relationship between the transcriptional expression values of the SEMA3F-NRP2 genes and the risk of occult nodal metastases. Keywords: semaphorin-3F; neuropilin-2; occult lymph node metastases; elective neck dissection; head and neck squamous cell carcinoma; HNSCC; lymphangiogenesis Author: Carla Meler-Claramonte, Francesc Xavier Avilés-Jurado, Isabel Vilaseca, Ximena Terra, Paloma Bragado, Gemma Fuster, Xavier León Vintró and Mercedes Camacho Creator: LaTeX with hyperref Producer: pdfTeX-1.40.21 CreationDate: Sat May 7 09:44:54 2022 CEST ModDate: Sat May 7 09:52:49 2022 CEST Tagged: no UserProperties: no Suspects: no Form: none JavaScript: no Pages: 12 Encrypted: no Page size: 595.276 x 841.89 pts (A4) Page rot: 0 File size: 686863 bytes Optimized: no PDF version: 1.7 name type encoding emb sub uni object ID ------------------------------------ ----------------- ---------------- --- --- --- --------- SXQPWE+VnURWPalladioL Type 1 Custom yes yes no 10 0 QCAVEE+URWPalladioL-Bold Type 1 Custom yes yes no 15 0 DSGKXT+URWPalladioL-Roma Type 1 Custom yes yes no 20 0 TCRAQO+URWPalladioL-Ital Type 1 Custom yes yes no 24 0 HJOMHV+VnURWPalladioL-Bold Type 1 Custom yes yes no 28 0 LCJEVJ+TimesNewRomanPS-BoldItalicMT Type 1C WinAnsi yes yes no 47 0 MOSBKF+URWPalladioL-BoldItal Type 1 Custom yes yes no 72 0 AHSHLD+CMSY10 Type 1 Builtin yes yes no 79 0 BCDEEE+PalatinoLinotype-Bold TrueType WinAnsi yes yes no 94 0 BCDFEE+PalatinoLinotype-Roman TrueType WinAnsi yes yes no 98 0 BCDGEE+PalatinoLinotype-Italic TrueType WinAnsi yes yes no 102 0 BCDHEE+PalatinoLinotype-Roman CID TrueType Identity-H yes yes yes 106 0 BCDJEE+PalatinoLinotype-BoldItalic TrueType WinAnsi yes yes no 113 0 Jhove (Rel. 1.28.0, 2023-05-18) Date: 2023-12-01 02:34:49 CET RepresentationInformation: 284193.pdf ReportingModule: PDF-hul, Rel. 1.12.4 (2023-03-16) LastModified: 2023-11-30 15:38:03 CET Size: 686863 Format: PDF Version: 1.7 Status: Well-Formed and valid SignatureMatches: PDF-hul MIMEtype: application/pdf PDFMetadata: Objects: 288 FreeObjects: 1 IncrementalUpdates: 0 DocumentCatalog: PageLayout: SinglePage PageMode: UseNone Outlines: Item: Title: Introduction Destination: section.1 Item: Title: Materials and Methods Destination: section.2 Children: Item: Title: Patients Destination: subsection.2.1 Item: Title: Transcriptional Analysis Destination: subsection.2.2 Item: Title: External Validation Sutyd: The Cancer Genome Atlas Database Destination: subsection.2.3 Item: Title: Statistical Analyses Destination: subsection.2.4 Item: Title: Results Destination: section.3 Children: Item: Title: SEMA3F/NRP2 Expression According to Clinical Variables Destination: subsection.3.1 Item: Title: Classification Depending on the SEMA3F/NRP2 Expression Destination: subsection.3.2 Item: Title: Results of the External Validation Study with the TCGA Data Destination: subsection.3.3 Item: Title: Discussion Destination: section.4 Item: Title: Conclusions Destination: section.5 Item: Title: References Destination: section.5 Info: Title: Semaphorin-3F/Neuropilin-2 Transcriptional Expression as a Predictive Biomarker of Occult Lymph Node Metastasesin HNSCC Author: Carla Meler-Claramonte, Francesc Xavier Avilés-Jurado, Isabel Vilaseca, Ximena Terra, Paloma Bragado, Gemma Fuster, Xavier León Vintró and Mercedes Camacho Subject: The expression of the semaphorin-3F (SEMA3F) and neuropilin-2 (NRP2) is involved in the regulation of lymphangiogenesis. The present study analyzes the relationship between the transcriptional expression of the SEMA3F-NRP2 genes and the presence of occult lymph node metastases in patients with cN0 head and neck squamous cell carcinomas. We analyzed the transcriptional expression of SEMA3F and NRP2 in a cohort of 53 patients with cN0 squamous cell carcinoma treated with an elective neck dissection. Occult lymph node metastases were found in 37.7% of the patients. Patients with occult lymph node metastases (cN0/pN+) had significantly lower SEMA3F expression values than patients without lymph node involvement (cN0/pN0). Considering the expression of the SEMA3F-NRP2 genes, patients were classified into two groups according to the risk of occult nodal metastasis: Group 1 (n = 34), high SEMA3F/low NRP2 expression, with a low risk of occult nodal involvement (14.7% cN0/pN+); Group 2 (n = 19), low SEMA3F or high SEMA3F/high NRP2 expression, with a high risk of occult nodal involvement (78.9% cN0/pN+). Multivariate analysis showed that patients in Group 2 had a 26.2 higher risk of lymph node involvement than patients in Group 1. There was a significant relationship between the transcriptional expression values of the SEMA3F-NRP2 genes and the risk of occult nodal metastases. Keywords: semaphorin-3F; neuropilin-2; occult lymph node metastases; elective neck dissection; head and neck squamous cell carcinoma; HNSCC; lymphangiogenesis Creator: LaTeX with hyperref Producer: pdfTeX-1.40.21 CreationDate: Sat May 07 09:44:54 CEST 2022 ModDate: Sat May 07 09:52:49 CEST 2022 ID: 0xf152fe12edf6ce7655617866b8c210ba, 0xf152fe12edf6ce7655617866b8c210ba Filters: FilterPipeline: FlateDecode Fonts: Type1: Font: BaseFont: DSGKXT+URWPalladioL-Roma FontSubset: true FirstChar: 2 LastChar: 252 FontDescriptor: FontName: DSGKXT+URWPalladioL-Roma Flags: Symbolic FontBBox: -166, -283, 1021, 943 FontFile: true EncodingDictionary: Differences: true Font: BaseFont: TCRAQO+URWPalladioL-Ital FontSubset: true FirstChar: 2 LastChar: 122 FontDescriptor: FontName: TCRAQO+URWPalladioL-Ital Flags: Symbolic FontBBox: -170, -305, 1010, 941 FontFile: true EncodingDictionary: Differences: true Font: BaseFont: MOSBKF+URWPalladioL-BoldItal FontSubset: true FirstChar: 78 LastChar: 112 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