2ff4a76bda9c833763c4749805f60ec2 290603.pdf 50a1a79a57cc18be0efe3d000e71d77a216d9616 290603.pdf b943d3b037965621722930cfe4cef3b63efe166397b1cd9b218fa5c557726585 290603.pdf Title: Physical Activity and Sedentary Time in Pregnancy: An Exploratory Study on Oxidative Stress Markers in the Placenta of Women with Obesity Subject: Regular moderate-to-vigorous physical activity (MVPA) and reduced sedentary time (ST) improve maternal glucose metabolism in pregnancy. More MVPA and less ST outside pregnancy increase antioxidant capacity, hence, are beneficial in preventing oxidative stress. The placenta is the first line of defense for the fetus from an adverse maternal environment, including oxidative stress. However, effects of MVPA and ST on oxidative stress markers in the placenta are unknown. The purpose of this study was to assess the association of MVPA and ST in pregnancy with oxidative stress markers in placentas of overweight/obese women (BMI 29 kg/m2). MVPA and ST were objectively measured with accelerometers at <20 weeks, 24–27 and 35–37 weeks of gestation. Using linear Bayesian multilevel models, the associations of MVPA and ST (mean and changes) with mRNA expression of a panel of 11 oxidative stress related markers were assessed in 96 women. MVPA was negatively correlated with HSP70 mRNA expression in a sex-independent manner and with GCLM expression only in placentas of female fetuses. ST was positively associated with HO-1 mRNA expression in placentas of male neonates. None of the other markers were associated with MVPA or ST. We speculate that increasing MVPA and reducing ST attenuates the oxidative stress state in placentas of obese pregnant women. Keywords: obesity; placenta; oxidative stress; physical activity; sedentary behavior Author: Saghi Zafaranieh, Anna M. Dieberger, Barbara Leopold-Posch, Berthold Huppertz, Sebastian Granitzer, Markus Hengstschläger, Claudia Gundacker, Gernot Desoye, Mireille N. M. van Poppel and DALI Core Investigator Group Creator: LaTeX with hyperref Producer: pdfTeX-1.40.21 CreationDate: Thu May 5 05:59:04 2022 CEST ModDate: Thu May 5 06:03:05 2022 CEST Custom Metadata: no Metadata Stream: no Tagged: no UserProperties: no Suspects: no Form: none JavaScript: no Pages: 13 Encrypted: no Page size: 595.276 x 841.89 pts (A4) Page rot: 0 File size: 1343806 bytes Optimized: no PDF version: 1.7 name type encoding emb sub uni object ID ------------------------------------ ----------------- ---------------- --- --- --- --------- EWRXSA+URWPalladioL-Bold Type 1 Custom yes yes no 10 0 AZBAUJ+URWPalladioL-Roma Type 1 Custom yes yes no 15 0 XRHXJH+URWPalladioL-Ital Type 1 Custom yes yes no 19 0 CRNDTI+CMSY10 Type 1 Builtin yes yes no 23 0 EBFBTV+TimesNewRomanPS-BoldItalicMT Type 1C WinAnsi yes yes no 41 0 QPZFHW+EURM10 Type 1 Builtin yes yes no 69 0 LCNPCA+PalatinoLinotype,Bold TrueType WinAnsi yes yes no 85 0 LCNPCC+PalatinoLinotype TrueType WinAnsi yes yes no 88 0 LCNPDE+PalatinoLinotype,Italic TrueType WinAnsi yes yes no 91 0 LCNPCA+PalatinoLinotype,Bold TrueType WinAnsi yes yes no 105 0 LCNPCB+PalatinoLinotype CID TrueType Identity-H yes yes yes 108 0 LCNPCC+PalatinoLinotype TrueType WinAnsi yes yes no 114 0 LCNPDE+PalatinoLinotype,Italic TrueType WinAnsi yes yes no 117 0 HYYHEA+CMBSY10 Type 1 Builtin yes yes no 125 0 LCNPBP+PalatinoLinotype,Bold CID TrueType Identity-H yes yes yes 145 0 LCNPCA+PalatinoLinotype,Bold TrueType WinAnsi yes yes no 151 0 LCNPCB+PalatinoLinotype CID TrueType Identity-H yes yes yes 154 0 LCNPCC+PalatinoLinotype TrueType WinAnsi yes yes no 160 0 LCNPDE+PalatinoLinotype,Italic TrueType WinAnsi yes yes no 163 0 BOMHEM+VnURWPalladioL Type 1 Custom yes yes no 179 0 Jhove (Rel. 1.28.0, 2023-05-18) Date: 2024-05-10 02:42:54 CEST RepresentationInformation: 290603.pdf ReportingModule: PDF-hul, Rel. 1.12.4 (2023-03-16) LastModified: 2024-05-09 12:47:50 CEST Size: 1343806 Format: PDF Version: 1.7 Status: Well-Formed and valid SignatureMatches: PDF-hul MIMEtype: application/pdf PDFMetadata: Objects: 378 FreeObjects: 1 IncrementalUpdates: 0 DocumentCatalog: PageLayout: SinglePage PageMode: UseNone Outlines: Item: Title: Introduction Destination: section.1 Item: Title: Materials and Methods Destination: section.2 Children: Item: Title: DALI Study Destination: subsection.2.1 Item: Title: Data Collection Destination: subsection.2.2 Item: Title: Physical Activity and Sedentary Time Destination: subsection.2.3 Item: Title: Placental Tissue Collection and RNA Isolation Destination: subsection.2.4 Item: Title: Statistical Methods Destination: subsection.2.5 Item: Title: Results Destination: section.3 Children: Item: Title: Physical Activity and Sedentary Time throughout Pregnancy Destination: subsection.3.1 Item: Title: Association of MVPA and ST with Oxidative Markers in the Placenta Destination: subsection.3.2 Item: Title: Sex-Specific Associations of MVPA and ST with Oxidative Markers in the Placenta Destination: subsection.3.3 Item: Title: Discussion Destination: section.4 Item: Title: Conclusions Destination: section.5 Item: Title: References Destination: section.5 Info: Title: Physical Activity and Sedentary Time in Pregnancy: An Exploratory Study on Oxidative Stress Markers in the Placenta of Women with Obesity Author: Saghi Zafaranieh, Anna M. Dieberger, Barbara Leopold-Posch, Berthold Huppertz, Sebastian Granitzer, Markus Hengstschläger, Claudia Gundacker, Gernot Desoye, Mireille N. M. van Poppel and DALI Core Investigator Group Subject: Regular moderate-to-vigorous physical activity (MVPA) and reduced sedentary time (ST) improve maternal glucose metabolism in pregnancy. More MVPA and less ST outside pregnancy increase antioxidant capacity, hence, are beneficial in preventing oxidative stress. The placenta is the first line of defense for the fetus from an adverse maternal environment, including oxidative stress. However, effects of MVPA and ST on oxidative stress markers in the placenta are unknown. The purpose of this study was to assess the association of MVPA and ST in pregnancy with oxidative stress markers in placentas of overweight/obese women (BMI 29 kg/m2). MVPA and ST were objectively measured with accelerometers at <20 weeks, 24 27 and 35 37 weeks of gestation. Using linear Bayesian multilevel models, the associations of MVPA and ST (mean and changes) with mRNA expression of a panel of 11 oxidative stress related markers were assessed in 96 women. MVPA was negatively correlated with HSP70 mRNA expression in a sex-independent manner and with GCLM expression only in placentas of female fetuses. ST was positively associated with HO-1 mRNA expression in placentas of male neonates. None of the other markers were associated with MVPA or ST. We speculate that increasing MVPA and reducing ST attenuates the oxidative stress state in placentas of obese pregnant women. 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