a06b64ddea631f0867e2cd24d0de5329 292416.pdf 3a74cb73c4925ef17d51b3ce9158c2cc1dc3e3c6 292416.pdf c33b9906c99c7a948863937434975482721229744a32445b7b92f837417b0c98 292416.pdf Title: Fine Control of In Vivo Magnetic Hyperthermia Using Iron Oxide Nanoparticles with Different Coatings and Degree of Aggregation Subject: The clinical implementation of magnetic hyperthermia has experienced little progress since the first clinical trial was completed in 2005. Some of the hurdles to overcome are the reliable production of magnetic nanoparticles with controlled properties and the control of the temperature at the target tissue in vivo. Here, forty samples of iron oxide superparamagnetic nanoparticles were prepared by similar methods and thoroughly characterized in terms of size, aggregation degree, and heating response. Selected samples were intratumorally administered in animals with subcutaneous xenografts of human pancreatic cancer. In vivo experiments showed that it is possible to control the rise in temperature by modulating the field intensity during in vivo magnetic hyperthermia protocols. The procedure does not require sophisticated materials and it can be easily implemented by researchers or practitioners working in magnetic hyperthermia therapies. Keywords: magnetic hyperthermia; cancer; nanoparticles; controlled heat in vivo Author: Yurena Luengo, Zamira V. Díaz-Riascos, David García-Soriano, Francisco J. Teran, Emilio J. Artés-Ibáñez, Oihane Ibarrola, Álvaro Somoza, Rodolfo Miranda, Simó Schwartz, Jr., Ibane Abasolo and Gorka Salas Creator: LaTeX with hyperref Producer: pdfTeX-1.40.21 CreationDate: Fri Jul 22 10:35:58 2022 CEST ModDate: Fri Jul 22 10:54:27 2022 CEST Custom Metadata: no Metadata Stream: no Tagged: no UserProperties: no Suspects: no Form: none JavaScript: no Pages: 15 Encrypted: no Page size: 595.276 x 841.89 pts (A4) Page rot: 0 File size: 2750458 bytes Optimized: no PDF version: 1.7 name type encoding emb sub uni object ID ------------------------------------ ----------------- ---------------- --- --- --- --------- MOCBVF+VnURWPalladioL Type 1 Custom yes yes no 10 0 LOTYYI+URWPalladioL-Bold Type 1 Custom yes yes no 15 0 BXDIPV+URWPalladioL-Roma Type 1 Custom yes yes no 20 0 SFVLQC+URWPalladioL-Ital Type 1 Custom yes yes no 24 0 FFAYKL+VnURWPalladioL-Bold Type 1 Custom yes yes no 28 0 IAFAOG+TimesNewRomanPS-BoldItalicMT Type 1C WinAnsi yes yes no 47 0 KVEXVN+CMSY10 Type 1 Builtin yes yes no 75 0 OQIVQT+EURM10 Type 1 Builtin yes yes no 82 0 CIDFont+F3 CID TrueType Identity-H yes no yes 94 0 CIDFont+F1 CID TrueType Identity-H yes no yes 102 0 CIDFont+F2 CID TrueType Identity-H yes no yes 110 0 CIDFont+F3 CID TrueType Identity-H yes no yes 124 0 CIDFont+F1 CID TrueType Identity-H yes no yes 132 0 CIDFont+F2 CID TrueType Identity-H yes no yes 140 0 CIDFont+F3 CID TrueType Identity-H yes no yes 157 0 CIDFont+F1 CID TrueType Identity-H yes no yes 165 0 CIDFont+F2 CID TrueType Identity-H yes no yes 173 0 CIDFont+F3 CID TrueType Identity-H yes no yes 187 0 CIDFont+F1 CID TrueType Identity-H yes no yes 195 0 CIDFont+F2 CID TrueType Identity-H yes no yes 203 0 CIDFont+F3 CID TrueType Identity-H yes no yes 217 0 CIDFont+F1 CID TrueType Identity-H yes no yes 225 0 CIDFont+F2 CID TrueType Identity-H yes no yes 233 0 CIDFont+F3 CID TrueType Identity-H yes no yes 247 0 CIDFont+F1 CID TrueType Identity-H yes no yes 255 0 CIDFont+F2 CID TrueType Identity-H yes no yes 263 0 Jhove (Rel. 1.28.0, 2023-05-18) Date: 2024-05-31 02:36:54 CEST RepresentationInformation: 292416.pdf ReportingModule: PDF-hul, Rel. 1.12.4 (2023-03-16) LastModified: 2024-05-30 10:56:51 CEST Size: 2750458 Format: PDF Version: 1.7 Status: Well-Formed and valid SignatureMatches: PDF-hul MIMEtype: application/pdf PDFMetadata: Objects: 434 FreeObjects: 1 IncrementalUpdates: 0 DocumentCatalog: PageLayout: SinglePage PageMode: UseNone Outlines: Item: Title: Introduction Destination: section.1 Item: Title: Materials and Methods Destination: section.2 Children: Item: Title: Preparation and Characterization of the Nanoparticles Destination: subsection.2.1 Item: Title: Animal Experimentation Destination: subsection.2.2 Item: Title: Results and Discussion Destination: section.3 Children: Item: Title: Characterization of the Nanoparticles Destination: subsection.3.1 Children: Item: Title: Characterization of Uncoated Nanoparticles (NPs) Destination: subsubsection.3.1.1 Item: Title: Characterization of Dextran and Starch Coated NPs Destination: subsubsection.3.1.2 Item: Title: Influence of Aggregation on the SAR Values Destination: subsubsection.3.1.3 Item: Title: In Vivo Hyperthermia Experiments Destination: subsection.3.2 Item: Title: Conclusions Destination: section.4 Item: Title: References Destination: section.4 Info: Title: Fine Control of In Vivo Magnetic Hyperthermia Using Iron Oxide Nanoparticles with Different Coatings and Degree of Aggregation Author: Yurena Luengo, Zamira V. Díaz-Riascos, David García-Soriano, Francisco J. Teran, Emilio J. Artés-Ibáñez, Oihane Ibarrola, Álvaro Somoza, Rodolfo Miranda, Simó Schwartz, Jr., Ibane Abasolo and Gorka Salas Subject: The clinical implementation of magnetic hyperthermia has experienced little progress since the first clinical trial was completed in 2005. Some of the hurdles to overcome are the reliable production of magnetic nanoparticles with controlled properties and the control of the temperature at the target tissue in vivo. Here, forty samples of iron oxide superparamagnetic nanoparticles were prepared by similar methods and thoroughly characterized in terms of size, aggregation degree, and heating response. Selected samples were intratumorally administered in animals with subcutaneous xenografts of human pancreatic cancer. In vivo experiments showed that it is possible to control the rise in temperature by modulating the field intensity during in vivo magnetic hyperthermia protocols. The procedure does not require sophisticated materials and it can be easily implemented by researchers or practitioners working in magnetic hyperthermia therapies. Keywords: magnetic hyperthermia; cancer; nanoparticles; controlled heat in vivo Creator: LaTeX with hyperref Producer: pdfTeX-1.40.21 CreationDate: Fri Jul 22 10:35:58 CEST 2022 ModDate: Fri Jul 22 10:54:27 CEST 2022 ID: 0x7f94ffc680b007abbd464a258defe5ef, 0x7f94ffc680b007abbd464a258defe5ef Filters: FilterPipeline: FlateDecode Fonts: Type1: Font: BaseFont: OQIVQT+EURM10 FontSubset: true FirstChar: 13 LastChar: 22 FontDescriptor: FontName: OQIVQT+EURM10 Flags: Symbolic FontBBox: -32, -243, 1060, 720 FontFile: true Font: BaseFont: BXDIPV+URWPalladioL-Roma FontSubset: true FirstChar: 2 LastChar: 252 FontDescriptor: FontName: BXDIPV+URWPalladioL-Roma Flags: Symbolic FontBBox: -166, -283, 1021, 943 FontFile: true EncodingDictionary: Differences: true Font: BaseFont: SFVLQC+URWPalladioL-Ital FontSubset: true FirstChar: 44 LastChar: 122 FontDescriptor: FontName: SFVLQC+URWPalladioL-Ital Flags: Symbolic FontBBox: -170, -305, 1010, 941 FontFile: true EncodingDictionary: Differences: true Font: BaseFont: MOCBVF+VnURWPalladioL FontSubset: true FirstChar: 129 LastChar: 243 FontDescriptor: FontName: MOCBVF+VnURWPalladioL Flags: Symbolic FontBBox: -166, -283, 1021, 943 FontFile: true EncodingDictionary: Differences: true Font: BaseFont: KVEXVN+CMSY10 FontSubset: true FirstChar: 0 LastChar: 20 FontDescriptor: FontName: KVEXVN+CMSY10 Flags: Symbolic FontBBox: -29, -960, 1116, 775 FontFile: true Font: BaseFont: FFAYKL+VnURWPalladioL-Bold FontSubset: true FirstChar: 129 LastChar: 226 FontDescriptor: FontName: FFAYKL+VnURWPalladioL-Bold Flags: Symbolic FontBBox: -152, -301, 1000, 935 FontFile: true EncodingDictionary: Differences: true Font: BaseFont: LOTYYI+URWPalladioL-Bold FontSubset: true FirstChar: 3 LastChar: 241 FontDescriptor: FontName: LOTYYI+URWPalladioL-Bold Flags: Symbolic FontBBox: -152, -301, 1000, 935 FontFile: true EncodingDictionary: Differences: true Pages: Page: Sequence: 1 Annotations: Annotation: Subtype: Link Rect: 64, 323, 152, 332 Annotation: Subtype: Link Rect: 34, 311, 109, 320 Annotation: Subtype: Link Rect: 34, 164, 96, 186 Annotation: Subtype: Link Rect: 126, 90, 154, 99 Annotation: Subtype: Link Rect: 34, 78, 147, 87 Annotation: Subtype: Link Rect: 34, 756, 168, 792 Annotation: Subtype: Link Rect: 515, 756, 560, 792 Annotation: Subtype: Link Rect: 449, 642, 460, 655 Annotation: Subtype: Link Rect: 226, 629, 237, 643 Annotation: Subtype: Link Rect: 323, 629, 334, 643 Annotation: Subtype: Link Rect: 531, 629, 542, 643 Annotation: Subtype: Link Rect: 127, 619, 138, 630 Annotation: Subtype: Link Rect: 234, 619, 245, 630 Annotation: Subtype: Link Rect: 204, 197, 211, 206 ActionDest: 276 Annotation: Subtype: Link Rect: 442, 172, 449, 181 ActionDest: 276 Annotation: Subtype: Link Rect: 452, 134, 459, 143 ActionDest: 276 Annotation: Subtype: Link Rect: 483, 84, 490, 93 ActionDest: 276 Annotation: Subtype: Link Rect: 135, 17, 311, 28 Annotation: Subtype: Link Rect: 385, 17, 560, 28 Annotation: Subtype: Link Rect: 34, 444, 87, 463 Page: Sequence: 2 Annotations: Annotation: Subtype: Link Rect: 471, 723, 478, 732 ActionDest: 276 Annotation: Subtype: Link Rect: 478, 723, 485, 732 ActionDest: 276 Annotation: Subtype: Link Rect: 215, 673, 222, 682 ActionDest: 276 Annotation: Subtype: Link Rect: 356, 622, 363, 631 ActionDest: 276 Annotation: Subtype: Link Rect: 366, 622, 378, 631 ActionDest: 276 Annotation: Subtype: Link Rect: 532, 572, 544, 581 ActionDest: 276 Annotation: Subtype: Link Rect: 544, 572, 556, 581 ActionDest: 276 Annotation: Subtype: Link Rect: 487, 522, 499, 531 ActionDest: 279 Annotation: Subtype: Link Rect: 531, 497, 543, 506 ActionDest: 276 Annotation: Subtype: Link Rect: 544, 497, 556, 506 ActionDest: 279 Annotation: Subtype: Link Rect: 344, 447, 356, 456 ActionDest: 279 Annotation: Subtype: Link Rect: 203, 334, 215, 343 ActionDest: 279 Annotation: Subtype: Link Rect: 215, 334, 227, 343 ActionDest: 279 Annotation: Subtype: Link Rect: 271, 258, 283, 267 ActionDest: 279 Annotation: Subtype: Link Rect: 283, 258, 295, 267 ActionDest: 279 Annotation: Subtype: Link Rect: 210, 170, 222, 180 ActionDest: 279 Annotation: Subtype: Link Rect: 225, 171, 237, 180 ActionDest: 279 Annotation: Subtype: Link Rect: 203, 108, 215, 117 ActionDest: 279 Annotation: Subtype: Link Rect: 476, 70, 488, 79 ActionDest: 279 Page: Sequence: 3 Annotations: Annotation: Subtype: Link Rect: 512, 723, 524, 732 ActionDest: 276 Annotation: Subtype: Link Rect: 313, 445, 325, 455 ActionDest: 279 Annotation: Subtype: Link Rect: 301, 408, 313, 417 ActionDest: 279 Annotation: Subtype: Link Rect: 313, 408, 325, 417 ActionDest: 279 Annotation: Subtype: Link Rect: 236, 169, 248, 178 ActionDest: 279 Page: Sequence: 4 Annotations: Annotation: Subtype: Link Rect: 187, 733, 516, 745 Annotation: Subtype: Link Rect: 321, 396, 333, 406 ActionDest: 279 Annotation: Subtype: Link Rect: 341, 279, 348, 291 ActionDest: 89 Annotation: Subtype: Link Rect: 394, 279, 401, 291 ActionDest: 86 Page: Sequence: 5 Annotations: Annotation: Subtype: Link Rect: 245, 192, 257, 201 ActionDest: 279 Annotation: Subtype: Link Rect: 531, 104, 543, 113 ActionDest: 279 Annotation: Subtype: Link Rect: 544, 104, 556, 113 ActionDest: 279 Page: Sequence: 6 Annotations: Annotation: Subtype: Link Rect: 376, 720, 383, 732 ActionDest: 119 Annotation: Subtype: Link Rect: 311, 708, 318, 720 ActionDest: 119 Annotation: Subtype: Link Rect: 413, 708, 420, 720 ActionDest: 119 Annotation: Subtype: Link Rect: 454, 122, 466, 131 ActionDest: 276 Annotation: Subtype: Link Rect: 286, 94, 293, 106 ActionDest: 119 Page: Sequence: 7 Annotations: Annotation: Subtype: Link Rect: 247, 597, 259, 606 ActionDest: 279 Annotation: Subtype: Link Rect: 259, 597, 271, 606 ActionDest: 279 Annotation: Subtype: Link Rect: 196, 469, 203, 481 ActionDest: 152 Annotation: Subtype: Link Rect: 352, 446, 359, 455 ActionDest: 276 Annotation: Subtype: Link Rect: 360, 446, 372, 455 ActionDest: 276 Annotation: Subtype: Link Rect: 403, 431, 410, 443 ActionDest: 152 Annotation: Subtype: Link Rect: 440, 356, 447, 368 ActionDest: 152 Annotation: Subtype: Link Rect: 529, 321, 541, 330 ActionDest: 279 Annotation: Subtype: Link Rect: 544, 321, 556, 330 ActionDest: 282 Annotation: Subtype: Link Rect: 482, 296, 489, 305 ActionDest: 276 Annotation: Subtype: Link Rect: 199, 255, 206, 267 ActionDest: 152 Annotation: Subtype: Link Rect: 544, 195, 556, 204 ActionDest: 282 Annotation: Subtype: Link Rect: 396, 133, 408, 142 ActionDest: 282 Page: Sequence: 8 Annotations: Annotation: Subtype: Link Rect: 391, 166, 398, 178 ActionDest: 182 Page: Sequence: 9 Annotations: Annotation: Subtype: Link Rect: 479, 316, 487, 328 ActionDest: 212 Annotation: Subtype: Link Rect: 226, 216, 233, 228 ActionDest: 212 Page: Sequence: 10 Annotations: Annotation: Subtype: Link Rect: 218, 347, 225, 359 ActionDest: 242 Annotation: Subtype: Link Rect: 532, 297, 539, 309 ActionDest: 242 Annotation: Subtype: Link Rect: 335, 259, 342, 273 ActionDest: 242 Annotation: Subtype: Link Rect: 331, 237, 343, 246 ActionDest: 276 Annotation: Subtype: Link Rect: 533, 209, 539, 223 ActionDest: 242 Annotation: Subtype: Link Rect: 401, 184, 408, 197 ActionDest: 242 Annotation: Subtype: Link Rect: 458, 146, 465, 158 ActionDest: 242 Annotation: Subtype: Link Rect: 199, 134, 206, 146 ActionDest: 242 Page: Sequence: 11 Page: Sequence: 12 Annotations: Annotation: Subtype: Link Rect: 409, 607, 416, 619 ActionDest: 242 Annotation: Subtype: Link Rect: 525, 607, 532, 619 ActionDest: 242 Annotation: Subtype: Link Rect: 501, 510, 513, 519 ActionDest: 276 Annotation: Subtype: Link Rect: 536, 119, 560, 130 Annotation: Subtype: Link Rect: 165, 107, 414, 118 Page: Sequence: 13 Annotations: Annotation: Subtype: Link Rect: 267, 492, 561, 503 Annotation: Subtype: Link Rect: 262, 347, 299, 358 Annotation: Subtype: Link Rect: 306, 347, 342, 358 Annotation: Subtype: Link Rect: 248, 324, 286, 335 Annotation: Subtype: Link Rect: 59, 302, 96, 312 Annotation: Subtype: Link Rect: 352, 278, 390, 289 Annotation: Subtype: Link Rect: 473, 255, 510, 266 Annotation: Subtype: Link Rect: 150, 198, 187, 209 Annotation: Subtype: Link Rect: 193, 198, 229, 209 Annotation: Subtype: Link Rect: 59, 164, 96, 174 Annotation: Subtype: Link Rect: 59, 129, 96, 140 Annotation: Subtype: Link Rect: 419, 106, 456, 117 Annotation: Subtype: Link Rect: 316, 83, 354, 94 Annotation: Subtype: Link Rect: 188, 60, 226, 70 Page: Sequence: 14 Annotations: Annotation: Subtype: Link Rect: 405, 722, 443, 733 Annotation: Subtype: Link Rect: 449, 722, 485, 733 Annotation: Subtype: Link Rect: 450, 710, 488, 721 Annotation: Subtype: Link Rect: 494, 710, 530, 721 Annotation: Subtype: Link Rect: 95, 676, 133, 687 Annotation: Subtype: Link Rect: 139, 676, 175, 687 Annotation: Subtype: Link Rect: 59, 642, 96, 652 Annotation: Subtype: Link Rect: 158, 607, 195, 617 Annotation: Subtype: Link Rect: 241, 583, 279, 594 Annotation: Subtype: Link Rect: 439, 560, 477, 571 Annotation: Subtype: Link Rect: 187, 527, 225, 537 Annotation: Subtype: Link Rect: 477, 503, 514, 514 Annotation: Subtype: Link Rect: 407, 468, 445, 479 Annotation: Subtype: Link Rect: 451, 468, 487, 479 Annotation: Subtype: Link Rect: 149, 435, 186, 445 Annotation: Subtype: Link Rect: 192, 435, 228, 445 Annotation: Subtype: Link Rect: 520, 411, 557, 422 Annotation: Subtype: Link Rect: 135, 377, 173, 387 Annotation: Subtype: Link Rect: 179, 377, 215, 387 Annotation: Subtype: Link Rect: 59, 354, 96, 364 Annotation: Subtype: Link Rect: 144, 331, 182, 341 Annotation: Subtype: Link Rect: 486, 307, 524, 318 Annotation: Subtype: Link Rect: 104, 274, 142, 284 Annotation: Subtype: Link Rect: 95, 251, 132, 261 Annotation: Subtype: Link Rect: 378, 204, 416, 215 Annotation: Subtype: Link Rect: 380, 181, 417, 192 Annotation: Subtype: Link Rect: 423, 181, 459, 192 Annotation: Subtype: Link Rect: 154, 146, 191, 157 Annotation: Subtype: Link Rect: 104, 113, 142, 123 Annotation: Subtype: Link Rect: 153, 78, 191, 88 Page: Sequence: 15 Annotations: Annotation: Subtype: Link Rect: 425, 722, 463, 733 Annotation: Subtype: Link Rect: 479, 699, 517, 710 Annotation: Subtype: Link Rect: 161, 665, 199, 675 Checksum: 926d3a3c Type: CRC32 Checksum: a06b64ddea631f0867e2cd24d0de5329 Type: MD5 Checksum: 3a74cb73c4925ef17d51b3ce9158c2cc1dc3e3c6 Type: SHA-1 Checksum: c33b9906c99c7a948863937434975482721229744a32445b7b92f837417b0c98 Type: SHA-256