b518536537ff209664a181fbc8c4afa9 292662.pdf 15c47e8b0602514959c257832ada4a42b97ff457 292662.pdf 53e8bbcca978eb961708e49a4f3e4246b00466da05b761881b4bd8aa5138d986 292662.pdf Title: Vascular Calcification and the Gut and Blood Microbiome in Chronic Kidney Disease Patients on Peritoneal Dialysis: A Pilot Study Subject: Vascular calcification (VC) is a frequent condition in chronic kidney disease (CKD) and a well-established risk factor for the development of cardiovascular disease (CVD). Gut dysbiosis may contribute to CVD and inflammation in CKD patients. Nonetheless, the role of gut and blood microbiomes in CKD-associated VC remains unknown. Therefore, this pilot study aimed to explore the link between gut and blood microbiomes and VC in CKD patients on peritoneal dialysis (CKD-PD). Our results showed relative changes in specific taxa between CKD-PD patients with and without VC, namely Coprobacter, Coprococcus 3, Lactobacillus, and Eubacterium eligens group in the gut, and Cutibacterium, Pajaroellobacter, Devosia, Hyphomicrobium, and Pelomonas in the blood. An association between VC and all-cause mortality risk in CKD-PD patients was also observed, and patients with higher mortality risk corroborate the changes of Eubacterium eligens in the gut and Devosia genus in the blood. Although we did not find differences in uremic toxins, intestinal translocation markers, and inflammatory parameters among CKD-PD patients with and without VC, soluble CD14 (sCD14), a nonspecific marker of monocyte activation, positively correlated with VC severity. Therefore, gut Eubacterium eligens group, blood Devosia, and circulating sCD14 should be further explored as biomarkers for VC, CVD, and mortality risk in CKD. Keywords: chronic kidney disease; vascular calcification; gut microbiome; blood microbiome; mortality risk; sCD14 Author: Ana Merino-Ribas, Ricardo Araujo, Luciano Pereira, Joana Campos, Luísa Barreiros, Marcela A. Segundo, Nádia Silva, Carolina F. F. A. Costa, Janete Quelhas-Santos, Fábio Trindade, Inês Falcão-Pires, Ines Alencastre, Ioana Bancu Dumitrescu and Benedita Sampaio-Maia Creator: LaTeX with hyperref Producer: pdfTeX-1.40.21 CreationDate: Tue Jun 21 14:29:08 2022 CEST ModDate: Tue Jun 21 15:31:28 2022 CEST Custom Metadata: no Metadata Stream: no Tagged: no UserProperties: no Suspects: no Form: none JavaScript: no Pages: 16 Encrypted: no Page size: 595.276 x 841.89 pts (A4) Page rot: 0 File size: 2302489 bytes Optimized: no PDF version: 1.7 name type encoding emb sub uni object ID ------------------------------------ ----------------- ---------------- --- --- --- --------- YUOZDY+VnURWPalladioL Type 1 Custom yes yes no 10 0 SKRKQW+URWPalladioL-Bold Type 1 Custom yes yes no 15 0 GNEQDE+URWPalladioL-Roma Type 1 Custom yes yes no 20 0 EAYMLL+URWPalladioL-Ital Type 1 Custom yes yes no 24 0 DHGNHP+VnURWPalladioL-Bold Type 1 Custom yes yes no 28 0 IVVSWS+TimesNewRomanPS-BoldItalicMT Type 1C WinAnsi yes yes no 48 0 VVSYYB+EURM10 Type 1 Builtin yes yes no 78 0 NSABJD+CMSY10 Type 1 Builtin yes yes no 85 0 QLBIEB+URWPalladioL-BoldItal Type 1 Custom yes yes no 98 0 Jhove (Rel. 1.28.0, 2023-05-18) Date: 2024-05-31 02:36:48 CEST RepresentationInformation: 292662.pdf ReportingModule: PDF-hul, Rel. 1.12.4 (2023-03-16) LastModified: 2024-05-30 17:05:01 CEST Size: 2302489 Format: PDF Version: 1.7 Status: Well-Formed and valid SignatureMatches: PDF-hul MIMEtype: application/pdf PDFMetadata: Objects: 379 FreeObjects: 1 IncrementalUpdates: 0 DocumentCatalog: PageLayout: SinglePage PageMode: UseNone Outlines: Item: Title: Introduction Destination: section.1 Item: Title: Materials and Methods Destination: section.2 Children: Item: Title: Study Design, Subjects, and Sample Collection Destination: subsection.2.1 Item: Title: Sample Processing and Microbiome Analysis Destination: subsection.2.2 Item: Title: 16S rRNA Gene Sequence Analysis Destination: subsection.2.3 Item: Title: Biochemical Analysis Destination: subsection.2.4 Item: Title: Statistics Destination: subsection.2.5 Item: Title: Results Destination: section.3 Item: Title: Discussion Destination: section.4 Item: Title: Conclusions Destination: section.5 Item: Title: References Destination: figure.caption.6 Info: Title: Vascular Calcification and the Gut and Blood Microbiome in Chronic Kidney Disease Patients on Peritoneal Dialysis: A Pilot Study Author: Ana Merino-Ribas, Ricardo Araujo, Luciano Pereira, Joana Campos, Luísa Barreiros, Marcela A. Segundo, Nádia Silva, Carolina F. F. A. Costa, Janete Quelhas-Santos, Fábio Trindade, Inês Falcão-Pires, Ines Alencastre, Ioana Bancu Dumitrescu and Benedita Sampaio-Maia Subject: Vascular calcification (VC) is a frequent condition in chronic kidney disease (CKD) and a well-established risk factor for the development of cardiovascular disease (CVD). Gut dysbiosis may contribute to CVD and inflammation in CKD patients. Nonetheless, the role of gut and blood microbiomes in CKD-associated VC remains unknown. Therefore, this pilot study aimed to explore the link between gut and blood microbiomes and VC in CKD patients on peritoneal dialysis (CKD-PD). Our results showed relative changes in specific taxa between CKD-PD patients with and without VC, namely Coprobacter, Coprococcus 3, Lactobacillus, and Eubacterium eligens group in the gut, and Cutibacterium, Pajaroellobacter, Devosia, Hyphomicrobium, and Pelomonas in the blood. An association between VC and all-cause mortality risk in CKD-PD patients was also observed, and patients with higher mortality risk corroborate the changes of Eubacterium eligens in the gut and Devosia genus in the blood. Although we did not find differences in uremic toxins, intestinal translocation markers, and inflammatory parameters among CKD-PD patients with and without VC, soluble CD14 (sCD14), a nonspecific marker of monocyte activation, positively correlated with VC severity. Therefore, gut Eubacterium eligens group, blood Devosia, and circulating sCD14 should be further explored as biomarkers for VC, CVD, and mortality risk in CKD. 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