44aff9056d98f5ca54afc9e77bca418b pmc_35215062.pdf 5578eca8bc7e2504825f93c6b292ee769028c51b pmc_35215062.pdf 16e9f9c8b8c57977f0cbd0adeb88ba11e1b4c74e8c054d50cfc91707424950e2 pmc_35215062.pdf Title: Exploratory Study of the Frequency of Detection and Tissue Distribution of Porcine Circovirus 3 (PCV-3) in Pig Fetuses at Different Gestational Ages Subject: Porcine circovirus 3 (PCV-3) has been associated with several pig diseases. Despite the pathogenicity of this virus has not been completely clarified, reproductive disorders are consistently associated with its infection. The aim of the present work was to analyze the presence of PCV-3 DNA in tissues from pig fetuses from different gestational timepoints. The fetuses were obtained either from farms with no reproductive problems (NRP, n = 249; all of them from the last third of gestation) or from a slaughterhouse (S, n = 51; 49 of the second-third of gestation and 2 from the third one). Tissues collected included brain, heart, lung, kidney, and/or spleen. Overall, the frequency of detection of PCV-3 was significantly higher in fetuses from the last third of the gestation (69/251, 27.5%) when compared to those from the second-third (5/49, 10.2%), although the viral loads were not significantly different. Moreover, the frequency of detection in NRP fetuses (69/249, 27.7%) was significantly higher than in S ones (5/51, 9.8%). Furthermore, PCV-3 DNA was detected in all tissue types analyzed. In conclusion, the present study demonstrates a higher frequency of PCV-3 DNA detection in fetuses from late periods of the gestation and highlights wide organ distributions of the virus in pig fetuses. Keywords: porcine circovirus 3 (PCV-3); reproductive failure; aborted fetuses; stillborn; quantitative PCR; subclinical infection Author: Albert Ruiz, Viviane Saporiti, Eva Huerta, Mònica Balasch, Joaquim Segalés and Marina Sibila Creator: LaTeX with hyperref Producer: pdfTeX-1.40.21 CreationDate: Thu Jan 20 07:15:22 2022 CET ModDate: Thu Jan 20 07:20:18 2022 CET Custom Metadata: no Metadata Stream: no Tagged: no UserProperties: no Suspects: no Form: none JavaScript: no Pages: 7 Encrypted: no Page size: 595.276 x 841.89 pts (A4) Page rot: 0 File size: 266681 bytes Optimized: no PDF version: 1.7 name type encoding emb sub uni object ID ------------------------------------ ----------------- ---------------- --- --- --- --------- XHDYZV+VnURWPalladioL Type 1 Custom yes yes no 10 0 XBSGCT+URWPalladioL-Bold Type 1 Custom yes yes no 15 0 HZMMKE+URWPalladioL-Roma Type 1 Custom yes yes no 20 0 IJFBLV+URWPalladioL-Ital Type 1 Custom yes yes no 24 0 SYTRHT+URWPalladioL-BoldItal Type 1 Custom yes yes no 28 0 WEQVKZ+VnURWPalladioL-Bold Type 1 Custom yes yes no 32 0 MCBCCI+SourceSansRoman-Regular Type 1C Custom yes yes no 40 0 MCBCCM+SourceSansRoman.613wght Type 1C Custom yes yes no 44 0 MCBCCO+SourceSansRoman-Bold Type 1C Custom yes yes no 48 0 UXHOAG+TimesNewRomanPS-BoldItalicMT Type 1C WinAnsi yes yes no 61 0 NJAOJC+CMSY10 Type 1 Builtin yes yes no 83 0 OVNEXW+EURM10 Type 1 Builtin yes yes no 93 0 Jhove (Rel. 1.28.0, 2023-05-18) Date: 2024-05-07 04:34:33 CEST RepresentationInformation: pmc_35215062.pdf ReportingModule: PDF-hul, Rel. 1.12.4 (2023-03-16) LastModified: 2022-03-06 18:37:16 CET Size: 266681 Format: PDF Version: 1.7 Status: Well-Formed and valid SignatureMatches: PDF-hul MIMEtype: application/pdf PDFMetadata: Objects: 242 FreeObjects: 1 IncrementalUpdates: 0 DocumentCatalog: PageLayout: SinglePage PageMode: UseNone Outlines: Item: Title: Introduction Destination: section.1 Item: Title: Results Destination: section.2 Children: Item: Title: Proportion of PCV-3 qPCR Positive Fetuses from Different Gestational Ages Destination: subsection.2.1 Item: Title: Proportion of PCV-3 Positive Fetuses from Different Origins Destination: subsection.2.2 Item: Title: PCV-3 Detection in Fetal Tissues Destination: subsection.2.3 Item: Title: Discussion Destination: section.3 Item: Title: Materials and Methods Destination: section.4 Item: Title: Conclusions Destination: section.5 Item: Title: References Destination: section.5 Info: Title: Exploratory Study of the Frequency of Detection and Tissue Distribution of Porcine Circovirus 3 (PCV-3) in Pig Fetuses at Different Gestational Ages Author: Albert Ruiz, Viviane Saporiti, Eva Huerta, Mònica Balasch, Joaquim Segalés and Marina Sibila Subject: Porcine circovirus 3 (PCV-3) has been associated with several pig diseases. Despite the pathogenicity of this virus has not been completely clarified, reproductive disorders are consistently associated with its infection. The aim of the present work was to analyze the presence of PCV-3 DNA in tissues from pig fetuses from different gestational timepoints. The fetuses were obtained either from farms with no reproductive problems (NRP, n = 249; all of them from the last third of gestation) or from a slaughterhouse (S, n = 51; 49 of the second-third of gestation and 2 from the third one). Tissues collected included brain, heart, lung, kidney, and/or spleen. Overall, the frequency of detection of PCV-3 was significantly higher in fetuses from the last third of the gestation (69/251, 27.5%) when compared to those from the second-third (5/49, 10.2%), although the viral loads were not significantly different. Moreover, the frequency of detection in NRP fetuses (69/249, 27.7%) was significantly higher than in S ones (5/51, 9.8%). Furthermore, PCV-3 DNA was detected in all tissue types analyzed. In conclusion, the present study demonstrates a higher frequency of PCV-3 DNA detection in fetuses from late periods of the gestation and highlights wide organ distributions of the virus in pig fetuses. 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