bd37131b48e01d2f77d762f69febf3b7 blsf-19-00014.pdf 4d20c059c959e4119db8dc92604fb0cb616f1e44 blsf-19-00014.pdf a948c261e2e072cbb8897bf0fe3d66d8e5fe1ad60442cba90d25dba6c66855d7 blsf-19-00014.pdf Title: Intrinsic and Extrinsic Factors in the Bizarre Patterns Elicited in the Open-Field Test in Male and Female Mice with Normal and AD-Pathological Aging under Social or Isolated Housing Conditions "2279 Subject: Bizarre behaviors (BB) are elicited in animals during stressful conditions and represent behavioral markers of disease/sickness relevant to animal welfare. These behaviors are disregarded in most experimental screenings due to their low incidence and short duration, despite their translational value mimicking disruptive behaviors associated with neurological/psychiatric diseases. However, we previously reported experimental conditions with conspicuous BB incidence: (1) 3xTg-AD mice for Alzheimer’s disease vs. wild-types; (2) the open-field test (OF) vs. other tests; (3) +6 months of age; (4) female sex; (5) older age (+13 months) male 3xTg-AD mice also exhibit more circling behavior (BB-CB) than control mice; (6) early neonatal handling reduces BB; whereas (7) BB are enhanced by d-galactose-induced accelerated aging. In the present work, we studied the BB patterns elicited in the OF using a longitudinal design with sixty-six 13-month-old male/female mice with normal/AD-pathological aging under social/isolated housing conditions, and aimed to explore the effects of intrinsic (genotype, sex) and extrinsic (environment) factors (n = 7–8/group). BB were recorded in 39% of animals. Sex, re-testing at an older age, and genotype factors differentiated two BB patterns: higher circling behavior (BB-CB, n = 12) in wild-types and males, but higher backward movements (BB-BM, n = 14) in 3xTg-AD, female, and older age mice. Isolation increased the incidence of freezing, mostly in 3xTg-AD mice, and exerted a modulatory role in BB, but interaction effects with other factors led to a residual significance. The results point to BB-BM as the most pervasive BB pattern in this animal model, which is also sensitive to the progress of aging/disease. Keywords: bizarre behaviors; animal welfare; Alzheimer’s disease; aging; social isolation;gender perspective Author: Lydia Giménez-Llort and Miguel Llauradó-Sánchez Creator: LaTeX with hyperref Producer: pdfTeX-1.40.21 CreationDate: Fri Jan 6 08:48:03 2023 CET ModDate: Fri Jan 6 08:50:49 2023 CET Custom Metadata: no Metadata Stream: no Tagged: no UserProperties: no Suspects: no Form: none JavaScript: no Pages: 2 Encrypted: no Page size: 595.276 x 841.89 pts (A4) Page rot: 0 File size: 205982 bytes Optimized: no PDF version: 1.7 name type encoding emb sub uni object ID ------------------------------------ ----------------- ---------------- --- --- --- --------- ZZTQUO+VnURWPalladioL-Bold Type 1 Custom yes yes no 10 0 QKZKVH+VnURWPalladioL Type 1 Custom yes yes no 15 0 NHGSRP+URWPalladioL-Bold Type 1 Custom yes yes no 19 0 NEJSNJ+URWPalladioL-Roma Type 1 Custom yes yes no 24 0 TZMXPD+URWPalladioL-Ital Type 1 Custom yes yes no 28 0 Jhove (Rel. 1.28.0, 2023-05-18) Date: 2025-02-21 02:11:29 CET RepresentationInformation: blsf-19-00014.pdf ReportingModule: PDF-hul, Rel. 1.12.4 (2023-03-16) LastModified: 2025-02-20 07:46:53 CET Size: 205982 Format: PDF Version: 1.7 Status: Well-Formed and valid SignatureMatches: PDF-hul MIMEtype: application/pdf PDFMetadata: Objects: 85 FreeObjects: 1 IncrementalUpdates: 0 DocumentCatalog: PageLayout: SinglePage PageMode: UseNone Info: Title: Intrinsic and Extrinsic Factors in the Bizarre Patterns Elicited in the Open-Field Test in Male and Female Mice with Normal and AD-Pathological Aging under Social or Isolated Housing Conditions "2279 Author: Lydia Giménez-Llort and Miguel Llauradó-Sánchez Subject: Bizarre behaviors (BB) are elicited in animals during stressful conditions and represent behavioral markers of disease/sickness relevant to animal welfare. These behaviors are disregarded in most experimental screenings due to their low incidence and short duration, despite their translational value mimicking disruptive behaviors associated with neurological/psychiatric diseases. However, we previously reported experimental conditions with conspicuous BB incidence: (1) 3xTg-AD mice for Alzheimer’s disease vs. wild-types; (2) the open-field test (OF) vs. other tests; (3) +6 months of age; (4) female sex; (5) older age (+13 months) male 3xTg-AD mice also exhibit more circling behavior (BB-CB) than control mice; (6) early neonatal handling reduces BB; whereas (7) BB are enhanced by d-galactose-induced accelerated aging. In the present work, we studied the BB patterns elicited in the OF using a longitudinal design with sixty-six 13-month-old male/female mice with normal/AD-pathological aging under social/isolated housing conditions, and aimed to explore the effects of intrinsic (genotype, sex) and extrinsic (environment) factors (n = 7 8/group). BB were recorded in 39% of animals. Sex, re-testing at an older age, and genotype factors differentiated two BB patterns: higher circling behavior (BB-CB, n = 12) in wild-types and males, but higher backward movements (BB-BM, n = 14) in 3xTg-AD, female, and older age mice. Isolation increased the incidence of freezing, mostly in 3xTg-AD mice, and exerted a modulatory role in BB, but interaction effects with other factors led to a residual significance. The results point to BB-BM as the most pervasive BB pattern in this animal model, which is also sensitive to the progress of aging/disease. 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