3a72916a6f7a128fe7528d138dab5c9a pmc_35051003.pdf 45702f0458c0b0026f596ccf93fa90d6c3d2a24e pmc_35051003.pdf 5eadbfbbc3de4ff655ce2c870a1e62dea03073978ece83028b2d2f776c9c8a07 pmc_35051003.pdf Title: Effect of Diet Supplementation with the Mycotoxin Binder Montmorillonite on the Bioavailability of Vitamins in Dairy Cows Subject: The objective of this study was to determine the effect of the mycotoxin binder montmorillonite (MMT) supplemented in the diet of dairy cows on the bioavailability of vitamins A, D, E, B1 and B6. Six multiparous Holstein-Friesian cows were used in a crossover design with two periods. Treatments were a control diet with or without MMT. Vitamins were infused individually into the abomasum through the ruminal cannula. Blood samples were collected from the jugular vein at 0, 1, 2, 3, 4, 6, 9, 12, 24 and 48 h after the administration of each vitamin. Results showed that vitamin A reached maximal concentration (Tmax) at 5.3 h after dosing, the maximal concentration (Cmax) was 1.2 times higher than the basal concentration (Cbasal), and the area under the curve (AUC) was 739 arbitrary units. Vitamin B6 reached the Tmax at 13 h after dosing, the Cmax was 1.4 times higher than the Cbasal, and the AUC was 222 arbitrary units. No differences were observed in Cbasal, Tmax, Cmax and AUC of vitamin A and B6 between control vs. MMT-supplemented cows. Plasma concentrations of vitamins D, E and B1 had no concentration peaks, and were not affected by MMT addition. The lack of a response suggests that their plasma concentration may be tightly regulated. Results of this study do not show evidence that MMT affects the bioavailability of vitamins A and B6 in vivo. Keywords: mycotoxin binder; adsorption; bioavailability; vitamins; binding capacity Author: Abdelhacib Kihal, Cristina Marquès, María Rodríguez-Prado, Eduard Jose-Cunilleras and Sergio Calsamiglia Creator: LaTeX with hyperref Producer: pdfTeX-1.40.21 CreationDate: Sat Jan 1 07:13:04 2022 CET ModDate: Sat Jan 1 07:22:04 2022 CET Tagged: no UserProperties: no Suspects: no Form: none JavaScript: no Pages: 8 Encrypted: no Page size: 595.276 x 841.89 pts (A4) Page rot: 0 File size: 926984 bytes Optimized: no PDF version: 1.7 name type encoding emb sub uni object ID ------------------------------------ ----------------- ---------------- --- --- --- --------- OYGWHX+VnURWPalladioL Type 1 Custom yes yes no 10 0 RSLQKK+URWPalladioL-Bold Type 1 Custom yes yes no 15 0 AMTGYV+URWPalladioL-Roma Type 1 Custom yes yes no 20 0 VRVZYC+URWPalladioL-Ital Type 1 Custom yes yes no 24 0 LMOTSQ+VnURWPalladioL-Bold Type 1 Custom yes yes no 28 0 MCBCCI+SourceSansRoman-Regular Type 1C Custom yes yes no 36 0 MCBCCM+SourceSansRoman.613wght Type 1C Custom yes yes no 40 0 MCBCCO+SourceSansRoman-Bold Type 1C Custom yes yes no 44 0 DVMWWR+TimesNewRomanPS-BoldItalicMT Type 1C WinAnsi yes yes no 57 0 MLYEVU+MSAM10 Type 1 Builtin yes yes no 79 0 PQVFRT+CMSY10 Type 1 Builtin yes yes no 83 0 SQADDB+EURM10 Type 1 Builtin yes yes no 87 0 DJBNAE+PalatinoLinotype,Bold TrueType WinAnsi yes yes no 98 0 DJBNAF+PalatinoLinotype CID TrueType Identity-H yes yes yes 101 0 DJBNAG+PalatinoLinotype TrueType WinAnsi yes yes no 107 0 DJBNAI+PalatinoLinotype,Italic TrueType WinAnsi yes yes no 110 0 DJBOFB+TimesNewRoman CID TrueType Identity-H yes yes yes 113 0 Jhove (Rel. 1.22.1, 2019-04-17) Date: 2022-04-29 02:25:23 CEST RepresentationInformation: pmc_35051003.pdf ReportingModule: PDF-hul, Rel. 1.12.1 (2019-04-17) LastModified: 2022-01-24 03:33:45 CET Size: 926984 Format: PDF Version: 1.7 Status: Well-Formed and valid SignatureMatches: PDF-hul MIMEtype: application/pdf PDFMetadata: Objects: 253 FreeObjects: 1 IncrementalUpdates: 0 DocumentCatalog: PageLayout: SinglePage PageMode: UseNone Outlines: Item: Title: Introduction Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@7b69c6ba Item: Title: Results Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@46daef40 Item: Title: Discussion Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@12f41634 Item: Title: Conclusions Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@13c27452 Item: Title: Materials and Methods Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@262b2c86 Children: Item: Title: Animals and Diet Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@371a67ec Item: Title: Experimental Design and Sample Collection Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@5ed828d Item: Title: Curve Fitting and Statistical Analysis Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@50d0686 Item: Title: References Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@7a3d45bd Info: Title: Effect of Diet Supplementation with the Mycotoxin Binder Montmorillonite on the Bioavailability of Vitamins in Dairy Cows Author: Abdelhacib Kihal, Cristina Marquès, María Rodríguez-Prado, Eduard Jose-Cunilleras and Sergio Calsamiglia Subject: The objective of this study was to determine the effect of the mycotoxin binder montmorillonite (MMT) supplemented in the diet of dairy cows on the bioavailability of vitamins A, D, E, B1 and B6. Six multiparous Holstein-Friesian cows were used in a crossover design with two periods. Treatments were a control diet with or without MMT. Vitamins were infused individually into the abomasum through the ruminal cannula. Blood samples were collected from the jugular vein at 0, 1, 2, 3, 4, 6, 9, 12, 24 and 48 h after the administration of each vitamin. Results showed that vitamin A reached maximal concentration (Tmax) at 5.3 h after dosing, the maximal concentration (Cmax) was 1.2 times higher than the basal concentration (Cbasal), and the area under the curve (AUC) was 739 arbitrary units. Vitamin B6 reached the Tmax at 13 h after dosing, the Cmax was 1.4 times higher than the Cbasal, and the AUC was 222 arbitrary units. No differences were observed in Cbasal, Tmax, Cmax and AUC of vitamin A and B6 between control vs. MMT-supplemented cows. Plasma concentrations of vitamins D, E and B1 had no concentration peaks, and were not affected by MMT addition. The lack of a response suggests that their plasma concentration may be tightly regulated. Results of this study do not show evidence that MMT affects the bioavailability of vitamins A and B6 in vivo. 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