12f5b136c94a6c4d82d2b7a46af1ebc9 pmc_35056584.pdf 3e21189815ade82fb7aea816dfbc50dd36380c80 pmc_35056584.pdf 28edd09e9e158307d7b52c5cc630a73ebd9b7ad437a6efb12f4d74aa92527b45 pmc_35056584.pdf Title: Experimental Mycobacterium microti Infection in Bank Voles (Myodes glareolus) Subject: Voles are maintenance hosts of Mycobacterium microti. In line with the goal to eradicate tuberculosis (TB) in livestock, the role of this mycobacteria needs to be assessed since it might interfere with current M. bovis/M. caprae surveillance strategies. To better understand the pathogenesis of TB in voles, an experimental infection model was set up to reproduce M. microti infection in laboratory Bank voles (Myodes glareolus). Two infection routes (intragastric and intraperitoneal) and doses (105 and 106 CFU/0.1 mL) were assessed. Voles were culled at different post-infection time points. Serology, histopathology, acid-fast bacilli staining, qPCR, and mycobacterial culture from tissues were performed. In addition, qPCR from feces and oral swabs were conducted to assess bacterial shedding. The model allowed us to faithfully reproduce the disease phenotype described in free-ranging voles and characterize the pathogenesis of the infection. Most animals showed multifocal and diffuse granulomatous lesions in the liver and spleen, respectively. Less frequently, granulomas were observed in lungs, lymph nodes, muscles, and salivary gland. Mycobacterial DNA was detected in feces from a few animals but not in oral swabs. However, one contact uninfected vole seroconverted and showed incipient TB compatible lesions, suggesting horizontal transmission between voles. Keywords: voles; wild rodents; tuberculosis; Mycobacterium microti; experimental infection Author: Enric Vidal, Judit Burgaya, Lorraine Michelet, Claudia Arrieta-Villegas, Guillermo Cantero, Krystel de Cruz, Jennifer Tambosco, Michelle Di Bari, Nonno Romolo, Maria Laura Boschiroli and Bernat Pérez de Val Creator: LaTeX with hyperref Producer: pdfTeX-1.40.21 CreationDate: Mon Jan 10 11:49:18 2022 CET ModDate: Mon Jan 10 11:56:33 2022 CET Tagged: no UserProperties: no Suspects: no Form: none JavaScript: no Pages: 15 Encrypted: no Page size: 595.276 x 841.89 pts (A4) Page rot: 0 File size: 4968381 bytes Optimized: no PDF version: 1.7 name type encoding emb sub uni object ID ------------------------------------ ----------------- ---------------- --- --- --- --------- OGLZWV+VnURWPalladioL Type 1 Custom yes yes no 10 0 BRQXFQ+URWPalladioL-Bold Type 1 Custom yes yes no 15 0 OCOULR+URWPalladioL-Roma Type 1 Custom yes yes no 20 0 WAPHYM+URWPalladioL-Ital Type 1 Custom yes yes no 24 0 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EBHOFG+PalatinoLinotype,Bold TrueType WinAnsi yes yes no 153 0 EBHOFI+PalatinoLinotype TrueType WinAnsi yes yes no 156 0 EBHOGJ+PalatinoLinotype,Italic CID TrueType Identity-H yes yes yes 159 0 EBHOGK+PalatinoLinotype,Italic TrueType WinAnsi yes yes no 165 0 EBHOFG+PalatinoLinotype,Bold TrueType WinAnsi yes yes no 175 0 EBHOFI+PalatinoLinotype TrueType WinAnsi yes yes no 178 0 EBHOGJ+PalatinoLinotype,Italic CID TrueType Identity-H yes yes yes 181 0 EBHOGK+PalatinoLinotype,Italic TrueType WinAnsi yes yes no 187 0 EBHOFG+PalatinoLinotype,Bold TrueType WinAnsi yes yes no 195 0 EBHOFI+PalatinoLinotype TrueType WinAnsi yes yes no 198 0 EBHOGJ+PalatinoLinotype,Italic CID TrueType Identity-H yes yes yes 201 0 EBHOGK+PalatinoLinotype,Italic TrueType WinAnsi yes yes no 207 0 EBHOFG+PalatinoLinotype,Bold TrueType WinAnsi yes yes no 218 0 EBHOFI+PalatinoLinotype TrueType WinAnsi yes yes no 221 0 EBHOGK+PalatinoLinotype,Italic TrueType WinAnsi yes yes no 224 0 Jhove (Rel. 1.22.1, 2019-04-17) Date: 2022-07-08 02:31:50 CEST RepresentationInformation: pmc_35056584.pdf ReportingModule: PDF-hul, Rel. 1.12.1 (2019-04-17) LastModified: 2022-01-31 03:35:48 CET Size: 4968381 Format: PDF Version: 1.7 Status: Well-Formed and valid SignatureMatches: PDF-hul MIMEtype: application/pdf PDFMetadata: Objects: 392 FreeObjects: 1 IncrementalUpdates: 0 DocumentCatalog: PageLayout: SinglePage PageMode: UseNone Outlines: Item: Title: Introduction Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@4923ab24 Item: Title: Materials and Methods Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@44c8afef Children: Item: Title: Experimental Animals and Housing Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@7b69c6ba Item: Title: Study Design and Experimental Infection Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@46daef40 Children: Item: Title: Experiment 1 Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@12f41634 Item: Title: Experiment 2 Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@13c27452 Item: Title: Ethics Statement Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@262b2c86 Item: Title: Postmortem Examination and Pathological Evaluation Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@371a67ec Item: Title: Mycobacterial Cultures Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@5ed828d Item: Title: Mycobacterial Load Assessment by qPCR Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@50d0686 Item: Title: Mycobacterial Excretion Assessment by qPCR Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@7a3d45bd Item: Title: Serology Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@1e7c7811 Item: Title: Data Analysis Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@77ec78b9 Item: Title: Results Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@1a3869f4 Children: Item: Title: Comparison of Infection Routes Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@a38d7a3 Item: Title: Comparison of IP Dosages Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@77f99a05 Item: Title: Disease Progression Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@63440df3 Item: Title: Horizontal Transmission Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@3aeaafa6 Item: Title: Discussion Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@76a3e297 Item: Title: Conclusions Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@4d3167f4 Item: Title: References Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@ed9d034 Info: Title: Experimental Mycobacterium microti Infection in Bank Voles (Myodes glareolus) Author: Enric Vidal, Judit Burgaya, Lorraine Michelet, Claudia Arrieta-Villegas, Guillermo Cantero, Krystel de Cruz, Jennifer Tambosco, Michelle Di Bari, Nonno Romolo, Maria Laura Boschiroli and Bernat Pérez de Val Subject: Voles are maintenance hosts of Mycobacterium microti. In line with the goal to eradicate tuberculosis (TB) in livestock, the role of this mycobacteria needs to be assessed since it might interfere with current M. bovis/M. caprae surveillance strategies. To better understand the pathogenesis of TB in voles, an experimental infection model was set up to reproduce M. microti infection in laboratory Bank voles (Myodes glareolus). Two infection routes (intragastric and intraperitoneal) and doses (105 and 106 CFU/0.1 mL) were assessed. Voles were culled at different post-infection time points. Serology, histopathology, acid-fast bacilli staining, qPCR, and mycobacterial culture from tissues were performed. In addition, qPCR from feces and oral swabs were conducted to assess bacterial shedding. The model allowed us to faithfully reproduce the disease phenotype described in free-ranging voles and characterize the pathogenesis of the infection. Most animals showed multifocal and diffuse granulomatous lesions in the liver and spleen, respectively. Less frequently, granulomas were observed in lungs, lymph nodes, muscles, and salivary gland. Mycobacterial DNA was detected in feces from a few animals but not in oral swabs. However, one contact uninfected vole seroconverted and showed incipient TB compatible lesions, suggesting horizontal transmission between voles. 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