d64dcd5320a408cf9d159b1a36b690aa pmc_35161583.pdf 1e563e3b2c4aa0f9f47e956b4b6fd45b7d50bbf7 pmc_35161583.pdf 0fe53d475e9f8c56cdcfe2b1f11283fdcc4609a74ad43f0e1c8ecbe7118b68bd pmc_35161583.pdf Title: Quantitative GC–TCD Measurements of Major Flatus Components: A Preliminary Analysis of the Diet Effect Subject: The impact of diet and digestive disorders in flatus composition remains largely unexplored. This is partially due to the lack of standardized sampling collection methods, and the easy atmospheric contamination. This paper describes a method to quantitatively determine the major gases in flatus and their application in a nutritional intervention. We describe how to direct sample flatus into Tedlar bags, and simultaneous analysis by gas chromatography–thermal conductivity detection (GC–TCD). Results are analyzed by univariate hypothesis testing and by multilevel principal component analysis. The reported methodology allows simultaneous determination of the five major gases with root mean measurement errors of 0.8% for oxygen (O2), 0.9% for nitrogen (N2), 0.14% for carbon dioxide (CO2), 0.11% for methane (CH4), and 0.26% for hydrogen (H2). The atmospheric contamination was limited to 0.86 (95% CI: [0.7–1.0])% for oxygen and 3.4 (95% CI: [1.4–5.3])% for nitrogen. As an illustration, the method has been successfully applied to measure the response to a nutritional intervention in a reduced crossover study in healthy subjects. Keywords: major flatus gas components; diet effect on flatus; multilevel principal component analysis; rectal gas collection Author: Rafael Freire, Marianela Mego, Luciana Fontes Oliveira, Silvia Mas, Fernando Azpiroz, Santiago Marco and Antonio Pardo Creator: LaTeX with hyperref Producer: pdfTeX-1.40.21 CreationDate: Sat Jan 22 11:13:12 2022 CET ModDate: Sat Jan 22 11:17:18 2022 CET Tagged: no UserProperties: no Suspects: no Form: none JavaScript: no Pages: 14 Encrypted: no Page size: 595.276 x 841.89 pts (A4) Page rot: 0 File size: 1390747 bytes Optimized: no PDF version: 1.7 name type encoding emb sub uni object ID ------------------------------------ ----------------- ---------------- --- --- --- --------- XBENPW+VnURWPalladioL Type 1 Custom yes yes no 10 0 NPBLIR+URWPalladioL-Bold Type 1 Custom yes yes no 15 0 RZZWXO+URWPalladioL-Roma Type 1 Custom yes yes no 20 0 KSCNCE+URWPalladioL-Ital Type 1 Custom yes yes no 24 0 MCBCCI+SourceSansRoman-Regular Type 1C Custom yes yes no 32 0 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Type 1 Builtin yes yes no 171 0 FNTSBS+PalatinoLinotype-Bold CID TrueType Identity-H yes yes yes 177 0 FNTSBS+PalatinoLinotype-Roman CID TrueType Identity-H yes yes yes 183 0 FNTSBS+PalatinoLinotype-Italic CID TrueType Identity-H yes yes yes 189 0 FNTSBS+PalatinoLinotype-Bold CID TrueType Identity-H yes yes yes 201 0 FNTSBS+PalatinoLinotype-Roman CID TrueType Identity-H yes yes yes 207 0 FNTSBS+PalatinoLinotype-Italic CID TrueType Identity-H yes yes yes 213 0 Jhove (Rel. 1.22.1, 2019-04-17) Date: 2023-06-10 02:39:20 CEST RepresentationInformation: pmc_35161583.pdf ReportingModule: PDF-hul, Rel. 1.12.1 (2019-04-17) LastModified: 2022-02-27 18:42:17 CET Size: 1390747 Format: PDF Version: 1.7 Status: Well-Formed and valid SignatureMatches: PDF-hul MIMEtype: application/pdf PDFMetadata: Objects: 428 FreeObjects: 1 IncrementalUpdates: 0 DocumentCatalog: PageLayout: SinglePage PageMode: UseNone Outlines: Item: Title: Introduction Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@44c8afef Item: Title: Materials and Methods Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@7b69c6ba Children: Item: Title: Participants Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@46daef40 Item: Title: Expèrimental Design Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@12f41634 Item: Title: Procedure Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@13c27452 Children: Item: Title: Dietary Interventions Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@262b2c86 Item: Title: Anal Gas Collection Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@371a67ec Item: Title: Chromatographic Protocol Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@5ed828d Item: Title: Standards for the Calibration Curve of the GC Instrument Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@50d0686 Item: Title: Data Processing Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@7a3d45bd Item: Title: Results and Discussion Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@1e7c7811 Children: Item: Title: Air Contamination Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@77ec78b9 Item: Title: Univariate Analysis According to Diet Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@1a3869f4 Item: Title: Multivariate Analysis on the Gas Composition of Flatus According to Diet Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@a38d7a3 Item: Title: Limitations of the Study and Future Research Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@77f99a05 Item: Title: Conclusions Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@63440df3 Item: Title: References Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@3aeaafa6 Info: Title: Quantitative GC TCD Measurements of Major Flatus Components: A Preliminary Analysis of the Diet Effect Author: Rafael Freire, Marianela Mego, Luciana Fontes Oliveira, Silvia Mas, Fernando Azpiroz, Santiago Marco and Antonio Pardo Subject: The impact of diet and digestive disorders in flatus composition remains largely unexplored. This is partially due to the lack of standardized sampling collection methods, and the easy atmospheric contamination. This paper describes a method to quantitatively determine the major gases in flatus and their application in a nutritional intervention. We describe how to direct sample flatus into Tedlar bags, and simultaneous analysis by gas chromatography thermal conductivity detection (GC TCD). Results are analyzed by univariate hypothesis testing and by multilevel principal component analysis. The reported methodology allows simultaneous determination of the five major gases with root mean measurement errors of 0.8% for oxygen (O2), 0.9% for nitrogen (N2), 0.14% for carbon dioxide (CO2), 0.11% for methane (CH4), and 0.26% for hydrogen (H2). The atmospheric contamination was limited to 0.86 (95% CI: [0.7 1.0])% for oxygen and 3.4 (95% CI: [1.4 5.3])% for nitrogen. As an illustration, the method has been successfully applied to measure the response to a nutritional intervention in a reduced crossover study in healthy subjects. 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