25f97abfaeab339933c6315d8c33c5ab pmc_35163449.pdf fd8daf0a21bf58d714f0377360f22629506d37e2 pmc_35163449.pdf ed22064002880c7efb252a06cd874cc4b3f9830e64a77cf9e57c36913fd913e8 pmc_35163449.pdf Title: Anti-Inflammatory Effect of an O-2-Substituted (1-3)–D-Glucan Produced by Pediococcus parvulus 2.6 in a Caco-2 PMA-THP-1 Co-Culture Model Subject: Bacterial -glucans are exopolysaccharides (EPSs), which can protect bacteria or cooperate in biofilm formation or in bacterial cell adhesion. Pediococcus parvulus 2.6 is a lactic acid bacterium that produces an O-2-substituted (1-3)–D-glucan. The structural similarity of this EPS to active compounds such as laminarin, together with its ability to modulate the immune system and to adhere in vitro to human enterocytes, led us to investigate, in comparison with laminarin, its potential as an immunomodulator of in vitro co-cultured Caco-2 and PMA-THP-1 cells. O-2-substituted (1-3)–D-glucan synthesized by the GTF glycosyl transferase of Pediococcus parvulus 2.6 or that by Lactococcus lactis NZ9000[pGTF] were purified and used in this study. The XTT tests revealed that all -glucans were non-toxic for both cell lines and activated PMA-THP-1 cells’ metabolisms. The O-2-substituted (1-3)–D-glucan modulated production and expression of IL-8 and the IL-10 in Caco-2 and PMA-THP-1 cells. Laminarin also modulated cytokine production by diminishing TNF- in Caco-2 cells and IL-8 in PMA-THP-1. All these features could be considered with the aim to produce function foods, supplemented with laminarin or with another novel -glucan-producing strain, in order to ameliorate an individual’s immune system response toward pathogens or to control mild side effects in remission patients affected by inflammatory bowel diseases. Keywords: (1-3)–D-glucan; bacterial exopolysaccharides; immunomodulators; Pediococcus parvulus; post-biotic; anti-inflammatory Author: Sara Notararigo, Encarnación Varela, Anna Otal, María Antolín, Francisco Guarner and Paloma López Creator: LaTeX with hyperref Producer: pdfTeX-1.40.21 CreationDate: Sat Jan 29 05:17:36 2022 CET ModDate: Sat Jan 29 05:32:51 2022 CET Tagged: no UserProperties: no Suspects: no Form: none JavaScript: no Pages: 20 Encrypted: no Page size: 595.276 x 841.89 pts (A4) Page rot: 0 File size: 1492997 bytes Optimized: no PDF version: 1.7 name type encoding emb sub uni object ID ------------------------------------ ----------------- ---------------- --- --- --- --------- ZYYJXI+EURB10 Type 1 Builtin yes yes no 10 0 YWWVQQ+VnURWPalladioL-Bold Type 1 Custom yes yes no 14 0 MOCBVF+VnURWPalladioL Type 1 Custom yes yes no 19 0 VVSYYB+EURM10 Type 1 Builtin yes yes no 23 0 LAQTUQ+URWPalladioL-Bold Type 1 Custom yes yes no 27 0 HFVBNF+URWPalladioL-Roma Type 1 Custom yes 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edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@12f41634 Item: Title: Effects of (1-3) D-Glucans on the Viability of PMA-THP-1 Cells Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@13c27452 Item: Title: Establishment of a Co-Culture Model with Caco-2 and PMA-THP-1 Cells under LPS Stimulation Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@262b2c86 Item: Title: Immunomodulation of O-2-Substituted (1-3) D-Glucan Produced by P. parvulus 2.6 on Cytokine Production in the Co-Cultured Model of Gut Mucosa Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@371a67ec Item: Title: Effect of O-2-Substituted (1-3) D-Glucan Produced by P. parvulus 2.6 on Gene Expression Profiles Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@5ed828d Item: Title: Materials and Methods Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@50d0686 Children: Item: Title: Cell Cultures Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@7a3d45bd Item: Title: EPS P and EPS L Production and Purification Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@1e7c7811 Item: Title: Cytotoxic Assay with XTT Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@77ec78b9 Item: Title: Co-Cultured In Vitro Gut Model of Caco-2 and PMA-THP-1 Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@1a3869f4 Item: Title: Determination of Cytokine Levels Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@a38d7a3 Item: Title: Total RNA Extraction and Analysis Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@77f99a05 Item: Title: Synthesis of cDNA from Total RNA Preparations Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@63440df3 Item: Title: Analysis of Gene Expression by Quantitative PCR Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@3aeaafa6 Item: Title: Statistical Analyses Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@76a3e297 Item: Title: Conclusions Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@4d3167f4 Item: Title: References Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@ed9d034 Info: Title: Anti-Inflammatory Effect of an O-2-Substituted (1-3) D-Glucan Produced by Pediococcus parvulus 2.6 in a Caco-2 PMA-THP-1 Co-Culture Model Author: Sara Notararigo, Encarnación Varela, Anna Otal, María Antolín, Francisco Guarner and Paloma López Subject: Bacterial -glucans are exopolysaccharides (EPSs), which can protect bacteria or cooperate in biofilm formation or in bacterial cell adhesion. Pediococcus parvulus 2.6 is a lactic acid bacterium that produces an O-2-substituted (1-3) D-glucan. The structural similarity of this EPS to active compounds such as laminarin, together with its ability to modulate the immune system and to adhere in vitro to human enterocytes, led us to investigate, in comparison with laminarin, its potential as an immunomodulator of in vitro co-cultured Caco-2 and PMA-THP-1 cells. O-2-substituted (1-3) D-glucan synthesized by the GTF glycosyl transferase of Pediococcus parvulus 2.6 or that by Lactococcus lactis NZ9000[pGTF] were purified and used in this study. The XTT tests revealed that all -glucans were non-toxic for both cell lines and activated PMA-THP-1 cells’ metabolisms. The O-2-substituted (1-3) D-glucan modulated production and expression of IL-8 and the IL-10 in Caco-2 and PMA-THP-1 cells. Laminarin also modulated cytokine production by diminishing TNF- in Caco-2 cells and IL-8 in PMA-THP-1. All these features could be considered with the aim to produce function foods, supplemented with laminarin or with another novel -glucan-producing strain, in order to ameliorate an individual’s immune system response toward pathogens or to control mild side effects in remission patients affected by inflammatory bowel diseases. 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