fdb93245ff6229a8db367051133e9fca pmc_35200320.pdf 90117d8b76955d7cf5103f7bd509f9e4eb1ac4ec pmc_35200320.pdf 9c8156d9f8b844928d161024098365ab04e81a5faccbf1e29b8bc92adf567103 pmc_35200320.pdf Title: A Sensitive Aptasensor Using Biotin-Streptavidin System for Patulin Detection in Apple Juice Subject: Patulin contamination in fruits, vegetables, and their products is considered a serious health risk factor for food safety and human health. Thus, a rapid, simple detection method for patulin is becoming important, which could provide a tool for routine screening and food surveys. The objective of this study was to develop a sensitive aptamer-based lateral flow assay (FLA) using Streptavidin functionalized gold nanoparticles for sensitive patulin detection. An excellent dynamic range for patulin detection was obtained (2.7~139.8 ng/mL in the buffer and 7.07~359.5 ng/mL in the sample) with no affinity for other mycotoxins such as zearalenone (ZEN), ochratoxin A (OTA), aflatoxin B1 (AFB1), citrinin or tenuazonic acid (TEA). The limit of detection was 0.19 ng/mL in the buffer and 0.36 ng/mL in the real sample. The recoveries were 83.3% to 107.1%, with a satisfactory RSD value from 6.5% to 7.5%. Hence the established LFA could be used as a rapid, simple, on-site screening tool for PAT determination in apple juice. Keywords: patulin; aptamer; lateral flow assay; on-site; apple juice Author: Xiaoqian Tang, Qi Zhang, Maria Isabel Pividori, Zhaowei Zhang, Jean-Louis Marty and Gaëlle Catanante Creator: LaTeX with hyperref Producer: pdfTeX-1.40.21 CreationDate: Tue Jan 25 04:44:13 2022 CET ModDate: Tue Jan 25 04:55:11 2022 CET Tagged: no UserProperties: no Suspects: no Form: none JavaScript: no Pages: 12 Encrypted: no Page size: 595.276 x 841.89 pts (A4) Page rot: 0 File size: 1094295 bytes Optimized: no PDF version: 1.7 name type encoding emb sub uni object ID ------------------------------------ ----------------- ---------------- --- --- --- --------- DDMOHK+VnURWPalladioL Type 1 Custom yes yes no 10 0 SCIVND+URWPalladioL-Bold Type 1 Custom yes yes no 15 0 NFTAVO+URWPalladioL-Roma Type 1 Custom yes yes no 20 0 KWQYTF+URWPalladioL-Ital Type 1 Custom yes yes no 24 0 MCBCCI+SourceSansRoman-Regular Type 1C Custom yes yes no 32 0 MCBCCM+SourceSansRoman.613wght Type 1C Custom yes yes no 36 0 MCBCCO+SourceSansRoman-Bold Type 1C Custom yes yes no 40 0 YHYESE+TimesNewRomanPS-BoldItalicMT Type 1C WinAnsi yes yes no 52 0 RGUFCP+CMSY10 Type 1 Builtin yes yes no 74 0 OVNEXW+EURM10 Type 1 Builtin yes yes no 78 0 GQNMRP+CMEX10 Type 1 Builtin yes yes no 91 0 JHYTSG+CMR10 Type 1 Builtin yes yes no 95 0 IDBPHL+PalatinoLinotype,Bold TrueType WinAnsi yes yes no 102 0 IDBPIM+PalatinoLinotype CID TrueType Identity-H yes yes yes 105 0 IDBPIN+PalatinoLinotype TrueType WinAnsi yes yes no 111 0 IDBPIP+PalatinoLinotype,Italic TrueType WinAnsi yes yes no 114 0 IDCBDH+TimesNewRoman CID TrueType Identity-H yes yes yes 117 0 IDCCLO+CambriaMath CID TrueType Identity-H yes yes yes 123 0 IDBPHL+PalatinoLinotype,Bold TrueType WinAnsi yes yes no 141 0 IDBPIN+PalatinoLinotype TrueType WinAnsi yes yes no 144 0 IDBPIP+PalatinoLinotype,Italic TrueType WinAnsi yes yes no 147 0 IDCBDH+TimesNewRoman CID TrueType Identity-H yes yes yes 150 0 Jhove (Rel. 1.22.1, 2019-04-17) Date: 2022-07-02 02:27:42 CEST RepresentationInformation: pmc_35200320.pdf ReportingModule: PDF-hul, Rel. 1.12.1 (2019-04-17) LastModified: 2022-02-27 18:36:12 CET Size: 1094295 Format: PDF Version: 1.7 Status: Well-Formed and valid SignatureMatches: PDF-hul MIMEtype: application/pdf PDFMetadata: Objects: 316 FreeObjects: 1 IncrementalUpdates: 0 DocumentCatalog: PageLayout: SinglePage PageMode: UseNone Outlines: Item: Title: Introduction Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@4923ab24 Item: Title: Experimental Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@44c8afef Children: Item: Title: Reagents, Materials, and Equipment Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@7b69c6ba Item: Title: Construction of the Lateral Flow Test Strips Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@46daef40 Item: Title: Aptamers and DNA Probes Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@12f41634 Item: Title: Developed Lateral Flow Test Strips for Patulin Detection Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@13c27452 Item: Title: Sample Preparation Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@262b2c86 Item: Title: Results and Discussion Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@371a67ec Children: Item: Title: Principle of Lateral Flow Strips Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@5ed828d Item: Title: Comparison and Selection of Aptamer Sequences and Competition Modes Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@50d0686 Item: Title: Comparison of Detection Methods Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@7a3d45bd Item: Title: Optimization of BIO-Aptamer and DIG-DNA Probe Ratio Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@1e7c7811 Item: Title: Optimization of Probe Concentration Immobilized on the Membrane Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@77ec78b9 Item: Title: Optimization of BSA and Cation Concentration in the Running Buffer Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@1a3869f4 Item: Title: Specificity and Sensitivity of LFA for Patulin Detection Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@a38d7a3 Item: Title: Validation of the LFA Method Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@77f99a05 Item: Title: Conclusions Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@63440df3 Item: Title: References Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@3aeaafa6 Info: Title: A Sensitive Aptasensor Using Biotin-Streptavidin System for Patulin Detection in Apple Juice Author: Xiaoqian Tang, Qi Zhang, Maria Isabel Pividori, Zhaowei Zhang, Jean-Louis Marty and Gaëlle Catanante Subject: Patulin contamination in fruits, vegetables, and their products is considered a serious health risk factor for food safety and human health. Thus, a rapid, simple detection method for patulin is becoming important, which could provide a tool for routine screening and food surveys. The objective of this study was to develop a sensitive aptamer-based lateral flow assay (FLA) using Streptavidin functionalized gold nanoparticles for sensitive patulin detection. An excellent dynamic range for patulin detection was obtained (2.7~139.8 ng/mL in the buffer and 7.07~359.5 ng/mL in the sample) with no affinity for other mycotoxins such as zearalenone (ZEN), ochratoxin A (OTA), aflatoxin B1 (AFB1), citrinin or tenuazonic acid (TEA). The limit of detection was 0.19 ng/mL in the buffer and 0.36 ng/mL in the real sample. The recoveries were 83.3% to 107.1%, with a satisfactory RSD value from 6.5% to 7.5%. Hence the established LFA could be used as a rapid, simple, on-site screening tool for PAT determination in apple juice. 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