4c563e4c703df2d7f4f0bdb150cba508 pmc_35203514.pdf b75018f48271f1313d63ae042ee94ca0fcfa899e pmc_35203514.pdf 248dc802ab1b72df2f60575d22f24503b575c93d7aa88020ce6dba2bdb8395ec pmc_35203514.pdf Title: Growth Factors Do Not Improve Muscle Function in Young or Adult mdx Mice Subject: Muscular dystrophies constitute a broad group of genetic disorders leading to muscle wasting. We have previously demonstrated that treating a muscular atrophy mouse model with growth factors resulted in increased muscle mass. In the present study, we treated the Duchenne mouse model mdx for 12 weeks with myogenic growth factors peri- and post-onset of muscular degeneration to explore the effects in the oxidative muscle soleus and the glycolytic muscle extensor digitorum longus (EDL). We found no overall beneficial effect in the peri-onset group at the conclusion of the study. In the post-onset group, the functional improvement by means of electrophysiological examinations ex vivo was mostly confined to the soleus. EDL benefitted from the treatment on a molecular level but did not improve functionally. Histopathology revealed signs of inflammation at the end of treatment. In conclusion, the growth factor cocktail failed to improve the mdx on a functional level. Keywords: mdx; DMD; muscular regeneration; hepatocyte growth factor; leukemia inhibitory factor Author: Tue L. Nielsen, Tessa M. Hornsyld, Tomàs Pinós, Camilla Brolin, John Vissing and Thomas O. Krag Creator: LaTeX with hyperref Producer: pdfTeX-1.40.21 CreationDate: Fri Jan 28 08:42:36 2022 CET ModDate: Fri Jan 28 09:05:17 2022 CET Tagged: no UserProperties: no Suspects: no Form: none JavaScript: no Pages: 16 Encrypted: no Page size: 595.276 x 841.89 pts (A4) Page rot: 0 File size: 3016665 bytes Optimized: no PDF version: 1.7 name type encoding emb sub uni object ID ------------------------------------ ----------------- ---------------- --- --- --- --------- UEHGBQ+VnURWPalladioL Type 1 Custom yes yes no 10 0 KRLPDM+URWPalladioL-Bold Type 1 Custom yes yes no 15 0 CPWOLP+URWPalladioL-Roma Type 1 Custom yes yes no 20 0 BAVCYR+URWPalladioL-Ital Type 1 Custom yes yes no 24 0 GRKUTL+URWPalladioL-BoldItal Type 1 Custom yes yes no 28 0 CVSFSJ+VnURWPalladioL-Bold Type 1 Custom yes yes no 32 0 MCBCCI+SourceSansRoman-Regular Type 1C Custom yes yes no 40 0 MCBCCM+SourceSansRoman.613wght Type 1C Custom yes yes no 44 0 MCBCCO+SourceSansRoman-Bold Type 1C Custom yes yes no 48 0 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Histochemical Analysis Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@5ed828d Item: Title: Immunohistochemistry Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@50d0686 Item: Title: Statistical Analysis Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@7a3d45bd Item: Title: Results Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@1e7c7811 Children: Item: Title: Body Mass Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@77ec78b9 Item: Title: Muscle Morphology Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@1a3869f4 Item: Title: The Effect of Treatment on Muscle Mass across Hind-Limb Muscles and Effect on Fiber-Type Composition Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@a38d7a3 Item: Title: Physiological Studies Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@77f99a05 Item: Title: Expression of Markers of Cell Division and Myogenic Signaling Proteins Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@63440df3 Item: Title: Histochemical Analysis of General Histology, Fibrosis, and Satellite-Cell Activation Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@3aeaafa6 Item: Title: Discussion Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@76a3e297 Item: Title: References Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@4d3167f4 Info: Title: Growth Factors Do Not Improve Muscle Function in Young or Adult mdx Mice Author: Tue L. Nielsen, Tessa M. Hornsyld, Tomàs Pinós, Camilla Brolin, John Vissing and Thomas O. Krag Subject: Muscular dystrophies constitute a broad group of genetic disorders leading to muscle wasting. We have previously demonstrated that treating a muscular atrophy mouse model with growth factors resulted in increased muscle mass. In the present study, we treated the Duchenne mouse model mdx for 12 weeks with myogenic growth factors peri- and post-onset of muscular degeneration to explore the effects in the oxidative muscle soleus and the glycolytic muscle extensor digitorum longus (EDL). We found no overall beneficial effect in the peri-onset group at the conclusion of the study. In the post-onset group, the functional improvement by means of electrophysiological examinations ex vivo was mostly confined to the soleus. EDL benefitted from the treatment on a molecular level but did not improve functionally. Histopathology revealed signs of inflammation at the end of treatment. In conclusion, the growth factor cocktail failed to improve the mdx on a functional level. 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